CA2536066A1 - Methods and kits for purifying his-tagged proteins - Google Patents
Methods and kits for purifying his-tagged proteins Download PDFInfo
- Publication number
- CA2536066A1 CA2536066A1 CA002536066A CA2536066A CA2536066A1 CA 2536066 A1 CA2536066 A1 CA 2536066A1 CA 002536066 A CA002536066 A CA 002536066A CA 2536066 A CA2536066 A CA 2536066A CA 2536066 A1 CA2536066 A1 CA 2536066A1
- Authority
- CA
- Canada
- Prior art keywords
- tagged
- protein
- solid support
- proteins
- zinc
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J45/00—Ion-exchange in which a complex or a chelate is formed; Use of material as complex or chelate forming ion-exchangers; Treatment of material for improving the complex or chelate forming ion-exchange properties
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Detergent Compositions (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US50254403P | 2003-09-12 | 2003-09-12 | |
| US60/502,544 | 2003-09-12 | ||
| US10/840,408 US7115397B2 (en) | 2003-09-12 | 2004-05-06 | Methods and kits for purifying his-tagged proteins |
| US10/840,408 | 2004-05-06 | ||
| PCT/US2004/029580 WO2005035092A1 (en) | 2003-09-12 | 2004-09-10 | Methods and kits for purifying his-tagged proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2536066A1 true CA2536066A1 (en) | 2005-04-21 |
Family
ID=34278838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002536066A Abandoned CA2536066A1 (en) | 2003-09-12 | 2004-09-10 | Methods and kits for purifying his-tagged proteins |
Country Status (8)
| Country | Link |
|---|---|
| US (4) | US7115397B2 (enExample) |
| EP (1) | EP1660206B1 (enExample) |
| JP (1) | JP2007505134A (enExample) |
| AT (1) | ATE403480T1 (enExample) |
| AU (1) | AU2004279361A1 (enExample) |
| CA (1) | CA2536066A1 (enExample) |
| DE (1) | DE602004015618D1 (enExample) |
| WO (1) | WO2005035092A1 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2005203321A1 (en) * | 2004-08-03 | 2006-02-23 | Axis-Shield Diagnostics Limited | Assay |
| US20070092943A1 (en) * | 2005-09-28 | 2007-04-26 | The University Of Chicago | Versatile vectors for expression of foreign proteins in photosynthetic bacteria |
| JP5656339B2 (ja) * | 2007-03-28 | 2015-01-21 | Jsr株式会社 | タンパク質固定化担体およびその製造方法 |
| US8815533B2 (en) | 2010-01-30 | 2014-08-26 | University Of Rochester | Method and system for purifying and quantitating proteins using heme fusion tags |
| US8796419B2 (en) | 2010-05-19 | 2014-08-05 | Hoffmann-La Roche Inc. | Hydrophobic interaction chromatography method |
| CN109569546B (zh) * | 2018-07-05 | 2021-05-18 | 华侨大学 | 一种用于组氨酸标签蛋白纯化的螯合金属磁性纳米颗粒的制备方法及应用 |
| US12392795B2 (en) | 2020-03-09 | 2025-08-19 | Idexx Laboratories, Inc. | Matrix and associated sample or mixing cup used for removing components of a liquid sample |
| US12366506B2 (en) | 2020-03-09 | 2025-07-22 | Idexx Laboratories, Inc. | Method for removing interfering components of a liquid sample prior to dispensing same on a chemical reagent test slide |
| CN119178879A (zh) * | 2023-06-21 | 2024-12-24 | 广东菲鹏生物有限公司 | 降低背景信号的方法 |
| WO2025028658A1 (ja) * | 2023-08-03 | 2025-02-06 | 国立大学法人東京農工大学 | 免疫増強剤及び免疫増強方法並びにタンパク質の凝集体及び／又は会合体の製造方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5840851A (en) | 1993-07-23 | 1998-11-24 | Plomer; J. Jeffrey | Purification of hemoglobin |
| US5962641A (en) | 1996-08-16 | 1999-10-05 | Clontech Laboratories, Inc. | Method for purification of recombinant proteins |
| WO1999057992A1 (en) | 1998-05-14 | 1999-11-18 | Clontech Laboratories, Inc. | Compositions and methods for protein purification based on a metal ion affinity site |
| US6232083B1 (en) * | 1999-03-12 | 2001-05-15 | The Research Foundation Of State University Of New York | Methods and compositions for screening cloned proteins |
| ATE320443T1 (de) * | 1999-07-15 | 2006-04-15 | Qiagen Gmbh | Verfahren zur trennung von teilchenförmigen substraten aus einer lösung indem man den teilchenverlust minimiert |
| WO2002037100A2 (en) | 2000-10-30 | 2002-05-10 | Lytton Simon D | Novel applications of nickel nitrilotriacetic acid (ni-nta) resin: hemeprotein removal, recovery, and purification from biological samples |
| CA2440530A1 (en) * | 2002-09-11 | 2004-03-11 | Henkel Kommanditgesellschaft Auf Aktien | Coating removal compositions |
| EP1581337A4 (en) | 2002-10-18 | 2008-05-07 | Promega Corp | COMPOSITION FOR DISCONNECTING MOLECULES |
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2004
- 2004-05-06 US US10/840,408 patent/US7115397B2/en not_active Expired - Fee Related
- 2004-09-10 WO PCT/US2004/029580 patent/WO2005035092A1/en not_active Ceased
- 2004-09-10 CA CA002536066A patent/CA2536066A1/en not_active Abandoned
- 2004-09-10 EP EP04788679A patent/EP1660206B1/en not_active Expired - Lifetime
- 2004-09-10 JP JP2006526315A patent/JP2007505134A/ja active Pending
- 2004-09-10 AT AT04788679T patent/ATE403480T1/de not_active IP Right Cessation
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2006
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| US7115397B2 (en) | 2006-10-03 |
| DE602004015618D1 (de) | 2008-09-18 |
| US20050209129A1 (en) | 2005-09-22 |
| AU2004279361A1 (en) | 2005-04-21 |
| EP1660206B1 (en) | 2008-08-06 |
| US20050059809A1 (en) | 2005-03-17 |
| JP2007505134A (ja) | 2007-03-08 |
| EP1660206A1 (en) | 2006-05-31 |
| ATE403480T1 (de) | 2008-08-15 |
| US20050209444A1 (en) | 2005-09-22 |
| US20060281906A1 (en) | 2006-12-14 |
| US7339036B2 (en) | 2008-03-04 |
| US7150973B2 (en) | 2006-12-19 |
| WO2005035092A1 (en) | 2005-04-21 |
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