JP2006525019A - 精子の染色方法 - Google Patents
精子の染色方法 Download PDFInfo
- Publication number
- JP2006525019A JP2006525019A JP2006509532A JP2006509532A JP2006525019A JP 2006525019 A JP2006525019 A JP 2006525019A JP 2006509532 A JP2006509532 A JP 2006509532A JP 2006509532 A JP2006509532 A JP 2006509532A JP 2006525019 A JP2006525019 A JP 2006525019A
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- JP
- Japan
- Prior art keywords
- sperm
- dye
- concentration
- staining
- mixture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000007447 staining method Methods 0.000 title description 2
- 238000010186 staining Methods 0.000 claims abstract description 84
- 238000000034 method Methods 0.000 claims abstract description 63
- 239000000975 dye Substances 0.000 claims description 114
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- PRDFBSVERLRRMY-UHFFFAOYSA-N 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole Chemical compound C1=CC(OCC)=CC=C1C1=NC2=CC=C(C=3NC4=CC(=CC=C4N=3)N3CCN(C)CC3)C=C2N1 PRDFBSVERLRRMY-UHFFFAOYSA-N 0.000 claims description 58
- 230000004899 motility Effects 0.000 claims description 52
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- 238000000684 flow cytometry Methods 0.000 abstract description 8
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
- C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Developmental Biology & Embryology (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Endocrinology (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Reproductive Health (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US45873103P | 2003-03-28 | 2003-03-28 | |
| PCT/US2004/009903 WO2004087177A1 (en) | 2003-03-28 | 2004-03-29 | Process for the staining of sperm |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2006525019A true JP2006525019A (ja) | 2006-11-09 |
| JP2006525019A5 JP2006525019A5 (https=) | 2007-05-24 |
Family
ID=33131822
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2006509532A Withdrawn JP2006525019A (ja) | 2003-03-28 | 2004-03-29 | 精子の染色方法 |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US7335507B2 (https=) |
| EP (1) | EP1608382B1 (https=) |
| JP (1) | JP2006525019A (https=) |
| CN (1) | CN100379429C (https=) |
| AT (1) | ATE369867T1 (https=) |
| AU (1) | AU2004226343B2 (https=) |
| BR (1) | BRPI0408852A (https=) |
| CA (1) | CA2519989A1 (https=) |
| DE (1) | DE602004008241T2 (https=) |
| MX (1) | MXPA05010478A (https=) |
| NZ (1) | NZ542688A (https=) |
| PL (1) | PL1608382T3 (https=) |
| WO (1) | WO2004087177A1 (https=) |
| ZA (1) | ZA200507823B (https=) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007531880A (ja) * | 2004-03-29 | 2007-11-08 | モンサント テクノロジー エルエルシー | 染色または仕分け方法において細胞内でおよび／または細胞外で酸化／還元反応を制御する組成物の使用 |
| JP2023547605A (ja) * | 2020-10-15 | 2023-11-13 | エービーエス グローバル インコーポレイテッド | ブタ雌雄鑑別精液及び使用方法 |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2264427B1 (en) | 1997-01-31 | 2017-05-03 | Xy, Llc | Optical apparatus with focussing reflector for converging radiation onto a flow of particles, and related method of analysis |
| US6071689A (en) | 1997-12-31 | 2000-06-06 | Xy, Inc. | System for improving yield of sexed embryos in mammals |
| US6149867A (en) * | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
| PT1917974E (pt) | 1998-07-30 | 2011-02-22 | Xy Llc | Sistema de inseminação artificial não cirúrgica em equinos |
| US7208265B1 (en) | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
| IL152714A (en) | 2000-05-09 | 2014-03-31 | Xy Llc | High-purity spermatozoa populations carrying chromosome-x and chromosome-y |
| CN1633259A (zh) * | 2000-06-12 | 2005-06-29 | Xy公司 | 利用分离的带x－染色体和带y－染色体的精子群的综合兽群管理系统 |
| US20040031071A1 (en) * | 2000-10-05 | 2004-02-12 | Xy, Inc. | System of hysteroscopic insemination of mares |
| WO2002043486A1 (en) | 2000-11-29 | 2002-06-06 | Xy, Inc. | System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations |
| US7713687B2 (en) | 2000-11-29 | 2010-05-11 | Xy, Inc. | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
| US8486618B2 (en) | 2002-08-01 | 2013-07-16 | Xy, Llc | Heterogeneous inseminate system |
| JP4595067B2 (ja) | 2002-08-01 | 2010-12-08 | エックスワイ，エルエルシー | 低圧精子細胞分離システム |
| CA2534394C (en) | 2002-08-15 | 2013-01-08 | Xy, Inc. | High resolution flow cytometer |
| US7169548B2 (en) * | 2002-09-13 | 2007-01-30 | Xy, Inc. | Sperm cell processing and preservation systems |
| BRPI0408857B1 (pt) | 2003-03-28 | 2018-09-11 | Inguran Llc | aparelho, métodos e processos para separar partículas e para prover esperma de animal separado por sexo |
| WO2004087177A1 (en) | 2003-03-28 | 2004-10-14 | Monsanto Technology Llc | Process for the staining of sperm |
| ES2541121T3 (es) | 2003-05-15 | 2015-07-16 | Xy, Llc | Clasificación eficiente de células haploides por sistemas de citometría de flujo |
| DK2801363T3 (en) * | 2004-03-29 | 2018-05-28 | Inguran Llc | PROCEDURE FOR STORING SORTED SPERMATOZOES |
| CA2574499C (en) * | 2004-07-22 | 2016-11-29 | Monsanto Technology Llc | Process for enriching a population of sperm cells |
| US7355696B2 (en) * | 2005-02-01 | 2008-04-08 | Arryx, Inc | Method and apparatus for sorting cells |
| US7618770B2 (en) | 2005-07-29 | 2009-11-17 | Xy, Inc. | Methods and apparatus for reducing protein content in sperm cell extenders |
| KR100949923B1 (ko) * | 2008-04-21 | 2010-03-30 | 중앙대학교 산학협력단 | 번식능력이 불량한 수퇘지 선별법 |
| US20110236923A1 (en) * | 2010-03-23 | 2011-09-29 | Genetics & Ivf Institute | Method for staining and sorting of a small volume of sperm |
| CA2794934A1 (en) * | 2010-04-01 | 2011-10-06 | Inguran, Llc | Methods and systems for reducing dna fragmentation in a processed sperm sample |
| CN101793641B (zh) * | 2010-04-14 | 2011-09-28 | 内蒙古蒙牛繁育生物技术股份有限公司 | 一种用于奶牛精子分离的荧光染色方法 |
| CN102095675B (zh) * | 2010-11-23 | 2012-05-23 | 内蒙古赛科星繁育生物技术股份有限公司 | 混合奶牛性控冻精产品鉴定和检测方法 |
| US9781919B2 (en) | 2011-06-01 | 2017-10-10 | Inguran, Llc | Compositions and methods for improving the quality of processed sperm |
| WO2012167151A1 (en) | 2011-06-01 | 2012-12-06 | Inguran, Llc | Compositions and methods for improving the quality of processed sperm |
| US9140688B2 (en) | 2011-09-30 | 2015-09-22 | Inguran, Llc | Sperm staining and sorting methods |
| US9433195B2 (en) | 2012-06-06 | 2016-09-06 | Inguran, Llc | Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells |
| US9888990B2 (en) | 2012-06-06 | 2018-02-13 | Inguran, Llc | Methods for use of sex sorted semen to improve genetic management in swine |
| US10620213B2 (en) | 2012-10-05 | 2020-04-14 | Inguran, Llc | High pressure sperm sorting and flow cytometer methods |
| CA2886782C (en) | 2012-10-05 | 2020-03-10 | Inguran, Llc | High efficiency methods of sex sorting sperm |
| CN103335934A (zh) * | 2013-06-28 | 2013-10-02 | 浙江星博生物科技有限公司 | 一种基于流式细胞术的精子活率检测试剂 |
| CN103344543B (zh) * | 2013-06-28 | 2016-07-06 | 浙江星博生物科技股份有限公司 | 一种基于流式细胞术的精子线粒体膜电位检测试剂 |
| CN103555662B (zh) * | 2013-10-31 | 2015-09-16 | 大连金弘基种畜有限公司 | 将四溴化1,1,2,2-四-[4-(2-三乙基乙氧基胺基)]苯乙烯应用于精子分离 |
| CN104749144A (zh) * | 2013-12-31 | 2015-07-01 | 深圳迈瑞生物医疗电子股份有限公司 | 血细胞检测试剂及血细胞处理方法和识别方法 |
| US11413358B2 (en) | 2018-04-09 | 2022-08-16 | Inguran, Llc | Sperm nuclei and methods of their manufacture and use |
| US10603075B1 (en) | 2018-11-30 | 2020-03-31 | Ohana Biosciences, Inc. | Compositions and methods for enhancing sperm function |
Family Cites Families (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3005756A (en) | 1958-11-14 | 1961-10-24 | Noland L Van Demark | Diluter containing carbon dioxide for preserving semen |
| US4474875A (en) | 1969-04-10 | 1984-10-02 | Wallace Shrimpton | Method and means for controlling the sex of mammalian offspring and product therefor |
| US3791384A (en) | 1971-07-15 | 1974-02-12 | Schaumann H | Artificial insemination of sows |
| US4267268A (en) | 1979-03-12 | 1981-05-12 | Nelson Jr Robert A | Spermatozoa extenders |
| US4362246A (en) | 1980-07-14 | 1982-12-07 | Adair Edwin Lloyd | Method of treating collected mammal semen and separating sperm into X Y components |
| GB2145112B (en) | 1983-04-27 | 1987-02-18 | Milk Marketing Board | Sorting living spermatozoa |
| CA2055494C (en) * | 1989-05-10 | 2000-01-18 | Lawrence Arthur Johnson | Method to preselect the sex of offspring |
| US5658751A (en) | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
| US5798276A (en) | 1995-06-07 | 1998-08-25 | Molecular Probes, Inc. | Reactive derivatives of sulforhodamine 101 with enhanced hydrolytic stability |
| US6040139A (en) | 1995-09-19 | 2000-03-21 | Bova; G. Steven | Laser cell purification system |
| AU722176B2 (en) | 1995-11-09 | 2000-07-27 | Medical Research Council | Use of CYB medium for the transportation and storage of sperm |
| US5985216A (en) | 1997-07-24 | 1999-11-16 | The United States Of America, As Represented By The Secretary Of Agriculture | Flow cytometry nozzle for high efficiency cell sorting |
| CA2304197C (en) | 1997-09-22 | 2009-02-03 | University Of Guelph | Reduction of sperm sensitivity to chilling |
| US6071689A (en) | 1997-12-31 | 2000-06-06 | Xy, Inc. | System for improving yield of sexed embryos in mammals |
| US6149867A (en) | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
| US6642018B1 (en) | 1998-03-27 | 2003-11-04 | Oncosis Llc | Method for inducing a response in one or more targeted cells |
| US6309815B1 (en) | 1998-08-07 | 2001-10-30 | University Of Kansas Medical Center | Composition and method for preparation, storage and activation of large populations of immotile sperm |
| AU3146800A (en) | 1999-03-05 | 2000-09-28 | De Danske Kvaegavlsforeninger | Determination of sperm concentration and viability for artificial insemination |
| FR2793495B1 (fr) | 1999-05-14 | 2001-08-10 | Imv Technologies | Dilueur pour la cryoconservation de spermatozoides de bovins |
| US7208265B1 (en) | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
| US6263745B1 (en) | 1999-12-03 | 2001-07-24 | Xy, Inc. | Flow cytometer nozzle and flow cytometer sample handling methods |
| IT1317724B1 (it) | 2000-01-14 | 2003-07-15 | Istituto Sperimentale Italiano | Procedimento per la produzione di embrioni non umani di sessopredeterminato ad alto valore genetico. |
| IL152714A (en) | 2000-05-09 | 2014-03-31 | Xy Llc | High-purity spermatozoa populations carrying chromosome-x and chromosome-y |
| WO2002019594A2 (en) | 2000-08-02 | 2002-03-07 | Arizona Board Of Regents, Acting On Behalf Of Arizona State University | Scanning fluorescence lifetime microscope: directed evolution |
| CA2454340A1 (en) | 2000-11-22 | 2002-05-30 | Pharmacia Corporation | Methods and apparatus for producing gender enriched sperm |
| WO2002043486A1 (en) | 2000-11-29 | 2002-06-06 | Xy, Inc. | System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations |
| WO2002077011A2 (en) * | 2001-03-12 | 2002-10-03 | The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon | Oxidation-reduction sensitive green fluorescent protein variants |
| WO2004087177A1 (en) | 2003-03-28 | 2004-10-14 | Monsanto Technology Llc | Process for the staining of sperm |
| BRPI0408857B1 (pt) | 2003-03-28 | 2018-09-11 | Inguran Llc | aparelho, métodos e processos para separar partículas e para prover esperma de animal separado por sexo |
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2004
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- 2004-03-29 JP JP2006509532A patent/JP2006525019A/ja not_active Withdrawn
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007531880A (ja) * | 2004-03-29 | 2007-11-08 | モンサント テクノロジー エルエルシー | 染色または仕分け方法において細胞内でおよび／または細胞外で酸化／還元反応を制御する組成物の使用 |
| JP2023547605A (ja) * | 2020-10-15 | 2023-11-13 | エービーエス グローバル インコーポレイテッド | ブタ雌雄鑑別精液及び使用方法 |
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| EP1608382A1 (en) | 2005-12-28 |
| AU2004226343A1 (en) | 2004-10-14 |
| ZA200507823B (en) | 2008-03-26 |
| BRPI0408852A (pt) | 2006-04-04 |
| US7335507B2 (en) | 2008-02-26 |
| US20050003472A1 (en) | 2005-01-06 |
| ATE369867T1 (de) | 2007-09-15 |
| CA2519989A1 (en) | 2004-10-14 |
| CN1795004A (zh) | 2006-06-28 |
| EP1608382B1 (en) | 2007-08-15 |
| MXPA05010478A (es) | 2005-12-15 |
| PL1608382T3 (pl) | 2008-03-31 |
| CN100379429C (zh) | 2008-04-09 |
| NZ542688A (en) | 2008-06-30 |
| DE602004008241D1 (de) | 2007-09-27 |
| DE602004008241T2 (de) | 2008-05-08 |
| WO2004087177A1 (en) | 2004-10-14 |
| AU2004226343B2 (en) | 2008-10-09 |
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