JP2006525019A5 - - Google Patents

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Publication number
JP2006525019A5
JP2006525019A5 JP2006509532A JP2006509532A JP2006525019A5 JP 2006525019 A5 JP2006525019 A5 JP 2006525019A5 JP 2006509532 A JP2006509532 A JP 2006509532A JP 2006509532 A JP2006509532 A JP 2006509532A JP 2006525019 A5 JP2006525019 A5 JP 2006525019A5
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JP
Japan
Prior art keywords
sperm
dye
stained
hoechst
measured
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
JP2006509532A
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English (en)
Japanese (ja)
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JP2006525019A (ja
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Publication date
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Priority claimed from PCT/US2004/009903 external-priority patent/WO2004087177A1/en
Publication of JP2006525019A publication Critical patent/JP2006525019A/ja
Publication of JP2006525019A5 publication Critical patent/JP2006525019A5/ja
Withdrawn legal-status Critical Current

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JP2006509532A 2003-03-28 2004-03-29 精子の染色方法 Withdrawn JP2006525019A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45873103P 2003-03-28 2003-03-28
PCT/US2004/009903 WO2004087177A1 (en) 2003-03-28 2004-03-29 Process for the staining of sperm

Publications (2)

Publication Number Publication Date
JP2006525019A JP2006525019A (ja) 2006-11-09
JP2006525019A5 true JP2006525019A5 (https=) 2007-05-24

Family

ID=33131822

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2006509532A Withdrawn JP2006525019A (ja) 2003-03-28 2004-03-29 精子の染色方法

Country Status (14)

Country Link
US (1) US7335507B2 (https=)
EP (1) EP1608382B1 (https=)
JP (1) JP2006525019A (https=)
CN (1) CN100379429C (https=)
AT (1) ATE369867T1 (https=)
AU (1) AU2004226343B2 (https=)
BR (1) BRPI0408852A (https=)
CA (1) CA2519989A1 (https=)
DE (1) DE602004008241T2 (https=)
MX (1) MXPA05010478A (https=)
NZ (1) NZ542688A (https=)
PL (1) PL1608382T3 (https=)
WO (1) WO2004087177A1 (https=)
ZA (1) ZA200507823B (https=)

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IL152714A (en) 2000-05-09 2014-03-31 Xy Llc High-purity spermatozoa populations carrying chromosome-x and chromosome-y
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US20040031071A1 (en) * 2000-10-05 2004-02-12 Xy, Inc. System of hysteroscopic insemination of mares
WO2002043486A1 (en) 2000-11-29 2002-06-06 Xy, Inc. System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
JP4595067B2 (ja) 2002-08-01 2010-12-08 エックスワイ，エルエルシー 低圧精子細胞分離システム
CA2534394C (en) 2002-08-15 2013-01-08 Xy, Inc. High resolution flow cytometer
US7169548B2 (en) * 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
BRPI0408857B1 (pt) 2003-03-28 2018-09-11 Inguran Llc aparelho, métodos e processos para separar partículas e para prover esperma de animal separado por sexo
WO2004087177A1 (en) 2003-03-28 2004-10-14 Monsanto Technology Llc Process for the staining of sperm
ES2541121T3 (es) 2003-05-15 2015-07-16 Xy, Llc Clasificación eficiente de células haploides por sistemas de citometría de flujo
DK2801363T3 (en) * 2004-03-29 2018-05-28 Inguran Llc PROCEDURE FOR STORING SORTED SPERMATOZOES
DE602005018907D1 (de) * 2004-03-29 2010-03-04 Inguran Llc Verwendung einer oxidations-/reduktionsreaktionen intrazellulär und/oder extrazellulär regulierenden zusammensetzung in einem anfärbe- oder sortierungsverfahren für spermatozoen
CA2574499C (en) * 2004-07-22 2016-11-29 Monsanto Technology Llc Process for enriching a population of sperm cells
US7355696B2 (en) * 2005-02-01 2008-04-08 Arryx, Inc Method and apparatus for sorting cells
US7618770B2 (en) 2005-07-29 2009-11-17 Xy, Inc. Methods and apparatus for reducing protein content in sperm cell extenders
KR100949923B1 (ko) * 2008-04-21 2010-03-30 중앙대학교 산학협력단 번식능력이 불량한 수퇘지 선별법
US20110236923A1 (en) * 2010-03-23 2011-09-29 Genetics & Ivf Institute Method for staining and sorting of a small volume of sperm
CA2794934A1 (en) * 2010-04-01 2011-10-06 Inguran, Llc Methods and systems for reducing dna fragmentation in a processed sperm sample
CN101793641B (zh) * 2010-04-14 2011-09-28 内蒙古蒙牛繁育生物技术股份有限公司 一种用于奶牛精子分离的荧光染色方法
CN102095675B (zh) * 2010-11-23 2012-05-23 内蒙古赛科星繁育生物技术股份有限公司 混合奶牛性控冻精产品鉴定和检测方法
US9781919B2 (en) 2011-06-01 2017-10-10 Inguran, Llc Compositions and methods for improving the quality of processed sperm
WO2012167151A1 (en) 2011-06-01 2012-12-06 Inguran, Llc Compositions and methods for improving the quality of processed sperm
US9140688B2 (en) 2011-09-30 2015-09-22 Inguran, Llc Sperm staining and sorting methods
US9433195B2 (en) 2012-06-06 2016-09-06 Inguran, Llc Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells
US9888990B2 (en) 2012-06-06 2018-02-13 Inguran, Llc Methods for use of sex sorted semen to improve genetic management in swine
US10620213B2 (en) 2012-10-05 2020-04-14 Inguran, Llc High pressure sperm sorting and flow cytometer methods
CA2886782C (en) 2012-10-05 2020-03-10 Inguran, Llc High efficiency methods of sex sorting sperm
CN103335934A (zh) * 2013-06-28 2013-10-02 浙江星博生物科技有限公司 一种基于流式细胞术的精子活率检测试剂
CN103344543B (zh) * 2013-06-28 2016-07-06 浙江星博生物科技股份有限公司 一种基于流式细胞术的精子线粒体膜电位检测试剂
CN103555662B (zh) * 2013-10-31 2015-09-16 大连金弘基种畜有限公司 将四溴化1,1,2,2-四-[4-(2-三乙基乙氧基胺基)]苯乙烯应用于精子分离
CN104749144A (zh) * 2013-12-31 2015-07-01 深圳迈瑞生物医疗电子股份有限公司 血细胞检测试剂及血细胞处理方法和识别方法
US11413358B2 (en) 2018-04-09 2022-08-16 Inguran, Llc Sperm nuclei and methods of their manufacture and use
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CN116348063A (zh) * 2020-10-15 2023-06-27 Abs全球公司 猪的选性精液和其使用方法

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