CN100379429C - 用于精子染色的方法 - Google Patents

用于精子染色的方法 Download PDF

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Publication number
CN100379429C
CN100379429C CNB200480014519XA CN200480014519A CN100379429C CN 100379429 C CN100379429 C CN 100379429C CN B200480014519X A CNB200480014519X A CN B200480014519XA CN 200480014519 A CN200480014519 A CN 200480014519A CN 100379429 C CN100379429 C CN 100379429C
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sperm
dye
concentration
temperature
mixture
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CN1795004A (zh
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M·安扎尔
C·L·路德维格
J·A·格雷厄姆
J·A·格伦策尔
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Inguran LLC
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Monsanto Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
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  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
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  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Endocrinology (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Reproductive Health (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
CNB200480014519XA 2003-03-28 2004-03-29 用于精子染色的方法 Expired - Lifetime CN100379429C (zh)

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US45873103P 2003-03-28 2003-03-28
US60/458,731 2003-03-28

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CN1795004A CN1795004A (zh) 2006-06-28
CN100379429C true CN100379429C (zh) 2008-04-09

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US (1) US7335507B2 (https=)
EP (1) EP1608382B1 (https=)
JP (1) JP2006525019A (https=)
CN (1) CN100379429C (https=)
AT (1) ATE369867T1 (https=)
AU (1) AU2004226343B2 (https=)
BR (1) BRPI0408852A (https=)
CA (1) CA2519989A1 (https=)
DE (1) DE602004008241T2 (https=)
MX (1) MXPA05010478A (https=)
NZ (1) NZ542688A (https=)
PL (1) PL1608382T3 (https=)
WO (1) WO2004087177A1 (https=)
ZA (1) ZA200507823B (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103555662A (zh) * 2013-10-31 2014-02-05 大连金弘基种畜有限公司 将四溴化1,1,2,2-四-[4-(2-三乙基乙氧基胺基)]苯乙烯应用于精子分离

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US6149867A (en) * 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
PT1917974E (pt) 1998-07-30 2011-02-22 Xy Llc Sistema de inseminação artificial não cirúrgica em equinos
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
IL152714A (en) 2000-05-09 2014-03-31 Xy Llc High-purity spermatozoa populations carrying chromosome-x and chromosome-y
CN1633259A (zh) * 2000-06-12 2005-06-29 Xy公司 利用分离的带x－染色体和带y－染色体的精子群的综合兽群管理系统
US20040031071A1 (en) * 2000-10-05 2004-02-12 Xy, Inc. System of hysteroscopic insemination of mares
WO2002043486A1 (en) 2000-11-29 2002-06-06 Xy, Inc. System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
JP4595067B2 (ja) 2002-08-01 2010-12-08 エックスワイ，エルエルシー 低圧精子細胞分離システム
CA2534394C (en) 2002-08-15 2013-01-08 Xy, Inc. High resolution flow cytometer
US7169548B2 (en) * 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
BRPI0408857B1 (pt) 2003-03-28 2018-09-11 Inguran Llc aparelho, métodos e processos para separar partículas e para prover esperma de animal separado por sexo
WO2004087177A1 (en) 2003-03-28 2004-10-14 Monsanto Technology Llc Process for the staining of sperm
ES2541121T3 (es) 2003-05-15 2015-07-16 Xy, Llc Clasificación eficiente de células haploides por sistemas de citometría de flujo
DK2801363T3 (en) * 2004-03-29 2018-05-28 Inguran Llc PROCEDURE FOR STORING SORTED SPERMATOZOES
DE602005018907D1 (de) * 2004-03-29 2010-03-04 Inguran Llc Verwendung einer oxidations-/reduktionsreaktionen intrazellulär und/oder extrazellulär regulierenden zusammensetzung in einem anfärbe- oder sortierungsverfahren für spermatozoen
CA2574499C (en) * 2004-07-22 2016-11-29 Monsanto Technology Llc Process for enriching a population of sperm cells
US7355696B2 (en) * 2005-02-01 2008-04-08 Arryx, Inc Method and apparatus for sorting cells
US7618770B2 (en) 2005-07-29 2009-11-17 Xy, Inc. Methods and apparatus for reducing protein content in sperm cell extenders
KR100949923B1 (ko) * 2008-04-21 2010-03-30 중앙대학교 산학협력단 번식능력이 불량한 수퇘지 선별법
US20110236923A1 (en) * 2010-03-23 2011-09-29 Genetics & Ivf Institute Method for staining and sorting of a small volume of sperm
CA2794934A1 (en) * 2010-04-01 2011-10-06 Inguran, Llc Methods and systems for reducing dna fragmentation in a processed sperm sample
CN101793641B (zh) * 2010-04-14 2011-09-28 内蒙古蒙牛繁育生物技术股份有限公司 一种用于奶牛精子分离的荧光染色方法
CN102095675B (zh) * 2010-11-23 2012-05-23 内蒙古赛科星繁育生物技术股份有限公司 混合奶牛性控冻精产品鉴定和检测方法
US9781919B2 (en) 2011-06-01 2017-10-10 Inguran, Llc Compositions and methods for improving the quality of processed sperm
WO2012167151A1 (en) 2011-06-01 2012-12-06 Inguran, Llc Compositions and methods for improving the quality of processed sperm
US9140688B2 (en) 2011-09-30 2015-09-22 Inguran, Llc Sperm staining and sorting methods
US9433195B2 (en) 2012-06-06 2016-09-06 Inguran, Llc Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells
US9888990B2 (en) 2012-06-06 2018-02-13 Inguran, Llc Methods for use of sex sorted semen to improve genetic management in swine
US10620213B2 (en) 2012-10-05 2020-04-14 Inguran, Llc High pressure sperm sorting and flow cytometer methods
CA2886782C (en) 2012-10-05 2020-03-10 Inguran, Llc High efficiency methods of sex sorting sperm
CN103335934A (zh) * 2013-06-28 2013-10-02 浙江星博生物科技有限公司 一种基于流式细胞术的精子活率检测试剂
CN103344543B (zh) * 2013-06-28 2016-07-06 浙江星博生物科技股份有限公司 一种基于流式细胞术的精子线粒体膜电位检测试剂
CN104749144A (zh) * 2013-12-31 2015-07-01 深圳迈瑞生物医疗电子股份有限公司 血细胞检测试剂及血细胞处理方法和识别方法
US11413358B2 (en) 2018-04-09 2022-08-16 Inguran, Llc Sperm nuclei and methods of their manufacture and use
US10603075B1 (en) 2018-11-30 2020-03-31 Ohana Biosciences, Inc. Compositions and methods for enhancing sperm function
CN116348063A (zh) * 2020-10-15 2023-06-27 Abs全球公司 猪的选性精液和其使用方法

Citations (2)

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WO2002041906A2 (en) * 2000-11-22 2002-05-30 Pharmacia Corporation Methods and apparatus for producing gender enriched sperm

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US4267268A (en) 1979-03-12 1981-05-12 Nelson Jr Robert A Spermatozoa extenders
US4362246A (en) 1980-07-14 1982-12-07 Adair Edwin Lloyd Method of treating collected mammal semen and separating sperm into X Y components
GB2145112B (en) 1983-04-27 1987-02-18 Milk Marketing Board Sorting living spermatozoa
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CA2304197C (en) 1997-09-22 2009-02-03 University Of Guelph Reduction of sperm sensitivity to chilling
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103555662A (zh) * 2013-10-31 2014-02-05 大连金弘基种畜有限公司 将四溴化1,1,2,2-四-[4-(2-三乙基乙氧基胺基)]苯乙烯应用于精子分离
CN103555662B (zh) * 2013-10-31 2015-09-16 大连金弘基种畜有限公司 将四溴化1,1,2,2-四-[4-(2-三乙基乙氧基胺基)]苯乙烯应用于精子分离

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EP1608382A1 (en) 2005-12-28
AU2004226343A1 (en) 2004-10-14
ZA200507823B (en) 2008-03-26
BRPI0408852A (pt) 2006-04-04
US7335507B2 (en) 2008-02-26
US20050003472A1 (en) 2005-01-06
ATE369867T1 (de) 2007-09-15
CA2519989A1 (en) 2004-10-14
CN1795004A (zh) 2006-06-28
EP1608382B1 (en) 2007-08-15
MXPA05010478A (es) 2005-12-15
JP2006525019A (ja) 2006-11-09
PL1608382T3 (pl) 2008-03-31
NZ542688A (en) 2008-06-30
DE602004008241D1 (de) 2007-09-27
DE602004008241T2 (de) 2008-05-08
WO2004087177A1 (en) 2004-10-14
AU2004226343B2 (en) 2008-10-09

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