JP2006511213A5 - - Google Patents
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- Publication number
- JP2006511213A5 JP2006511213A5 JP2004553727A JP2004553727A JP2006511213A5 JP 2006511213 A5 JP2006511213 A5 JP 2006511213A5 JP 2004553727 A JP2004553727 A JP 2004553727A JP 2004553727 A JP2004553727 A JP 2004553727A JP 2006511213 A5 JP2006511213 A5 JP 2006511213A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleotides
- pcr
- amplicon
- crna
- synthetic oligonucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108091034117 Oligonucleotide Proteins 0.000 claims description 43
- 238000000034 method Methods 0.000 claims description 42
- 239000002773 nucleotide Substances 0.000 claims description 37
- 125000003729 nucleotide group Chemical group 0.000 claims description 37
- 108091093088 Amplicon Proteins 0.000 claims description 32
- 238000013518 transcription Methods 0.000 claims description 24
- 230000035897 transcription Effects 0.000 claims description 24
- 238000000338 in vitro Methods 0.000 claims description 21
- 239000002299 complementary DNA Substances 0.000 claims description 19
- 238000011002 quantification Methods 0.000 claims description 15
- 238000003757 reverse transcription PCR Methods 0.000 claims description 12
- 108020004394 Complementary RNA Proteins 0.000 claims description 11
- 239000003184 complementary RNA Substances 0.000 claims description 11
- 238000010839 reverse transcription Methods 0.000 claims description 10
- 108020005029 5' Flanking Region Proteins 0.000 claims description 9
- 238000012360 testing method Methods 0.000 claims description 9
- 238000013207 serial dilution Methods 0.000 claims description 7
- 108020005065 3' Flanking Region Proteins 0.000 claims description 6
- 150000007523 nucleic acids Chemical class 0.000 claims description 6
- 230000002194 synthesizing effect Effects 0.000 claims description 6
- 108020004707 nucleic acids Proteins 0.000 claims description 4
- 102000039446 nucleic acids Human genes 0.000 claims description 4
- 241001515965 unidentified phage Species 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 description 26
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 25
- 239000000523 sample Substances 0.000 description 19
- 238000003786 synthesis reaction Methods 0.000 description 11
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 10
- 230000015572 biosynthetic process Effects 0.000 description 10
- 108020004999 messenger RNA Proteins 0.000 description 8
- 230000014509 gene expression Effects 0.000 description 7
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 6
- 238000003753 real-time PCR Methods 0.000 description 6
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 5
- 230000003321 amplification Effects 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 238000003199 nucleic acid amplification method Methods 0.000 description 5
- 238000013461 design Methods 0.000 description 4
- 239000013612 plasmid Substances 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 238000010804 cDNA synthesis Methods 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 210000002216 heart Anatomy 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 210000001541 thymus gland Anatomy 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 108010017826 DNA Polymerase I Proteins 0.000 description 1
- 102000004594 DNA Polymerase I Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108060002716 Exonuclease Proteins 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 230000006819 RNA synthesis Effects 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000006872 enzymatic polymerization reaction Methods 0.000 description 1
- 102000013165 exonuclease Human genes 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000012340 reverse transcriptase PCR Methods 0.000 description 1
- 238000004007 reversed phase HPLC Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical group CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/294,781 US20040096829A1 (en) | 2002-11-14 | 2002-11-14 | Absolute quantitation of nucleic acids by RT-PCR |
| PCT/US2003/036522 WO2004045522A2 (en) | 2002-11-14 | 2003-11-14 | Absolute quantitation of nucleic acids by rt-pcr |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2006511213A JP2006511213A (ja) | 2006-04-06 |
| JP2006511213A5 true JP2006511213A5 (enExample) | 2007-01-11 |
Family
ID=32297041
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004553727A Pending JP2006511213A (ja) | 2002-11-14 | 2003-11-14 | Ｒｔ−ｐｃｒによる核酸の絶対定量 |
Country Status (18)
| Country | Link |
|---|---|
| US (2) | US20040096829A1 (enExample) |
| EP (1) | EP1578378A4 (enExample) |
| JP (1) | JP2006511213A (enExample) |
| KR (1) | KR20050074620A (enExample) |
| CN (1) | CN1759190A (enExample) |
| AU (1) | AU2003297278A1 (enExample) |
| BR (1) | BR0316336A (enExample) |
| CA (1) | CA2505992A1 (enExample) |
| EA (1) | EA008742B1 (enExample) |
| GE (1) | GEP20074083B (enExample) |
| IS (1) | IS7853A (enExample) |
| MX (1) | MXPA05005168A (enExample) |
| NO (1) | NO20052862L (enExample) |
| NZ (1) | NZ540595A (enExample) |
| PL (1) | PL377027A1 (enExample) |
| RS (1) | RS20050365A (enExample) |
| WO (1) | WO2004045522A2 (enExample) |
| ZA (1) | ZA200504282B (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090075273A1 (en) * | 2005-11-14 | 2009-03-19 | Siemens Healthcare Diagnostics Inc. | Planar Waveguide Detection Chips and Chambers for Performing Multiple PCR Assays |
| CN100582241C (zh) * | 2007-10-18 | 2010-01-20 | 昆明云大生化科技有限责任公司 | 一种可制作标准曲线的临床定量检测基因芯片的制备方法 |
| US11091803B2 (en) * | 2014-04-14 | 2021-08-17 | W2 Biosolutions, Llc | Nucleic acid quantification method |
| TW201703406A (zh) * | 2015-04-14 | 2017-01-16 | 電源整合有限責任公司 | 切換裝置及功率模組 |
| CN109988846B (zh) * | 2019-03-11 | 2023-05-26 | 浙江省淡水水产研究所 | 一种适于红螯螯虾性腺组织mRNA石蜡切片原位杂交的方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1340807C (en) * | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| US5219727A (en) * | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
| US5543509A (en) * | 1992-08-14 | 1996-08-06 | The United States Of America As Represented By The Department Of Health And Human Services | Method for quantifying laminin and β-actin messenger RNA |
| ZA936015B (en) * | 1992-08-24 | 1994-03-10 | Akzo Nv | Elimination of false negatives in nuleic acid detection. |
| US6093542A (en) * | 1998-01-09 | 2000-07-25 | Akzo Nobel N.V. | Isothermal transcription based amplification assay for the detection and quantitation of macrophage derived chemokine RNA |
| US6365346B1 (en) * | 1998-02-18 | 2002-04-02 | Dade Behring Inc. | Quantitative determination of nucleic acid amplification products |
| DE60014762T2 (de) * | 1999-05-24 | 2005-10-13 | Tosoh Corp., Shinnanyo | Methode zum Nachweis von Ribonukleinsäuren |
| AU1601801A (en) * | 1999-11-16 | 2001-05-30 | Eric M. Eastman | Methods of preparing crna |
-
2002
- 2002-11-14 US US10/294,781 patent/US20040096829A1/en not_active Abandoned
-
2003
- 2003-05-20 IS IS7853A patent/IS7853A/is unknown
- 2003-11-14 MX MXPA05005168A patent/MXPA05005168A/es unknown
- 2003-11-14 BR BR0316336-9A patent/BR0316336A/pt not_active IP Right Cessation
- 2003-11-14 EA EA200500818A patent/EA008742B1/ru not_active IP Right Cessation
- 2003-11-14 EP EP03811624A patent/EP1578378A4/en not_active Withdrawn
- 2003-11-14 CA CA002505992A patent/CA2505992A1/en not_active Abandoned
- 2003-11-14 AU AU2003297278A patent/AU2003297278A1/en not_active Abandoned
- 2003-11-14 KR KR1020057008627A patent/KR20050074620A/ko not_active Withdrawn
- 2003-11-14 PL PL377027A patent/PL377027A1/pl not_active Application Discontinuation
- 2003-11-14 WO PCT/US2003/036522 patent/WO2004045522A2/en not_active Ceased
- 2003-11-14 GE GEAP20038842A patent/GEP20074083B/en unknown
- 2003-11-14 CN CNA2003801075043A patent/CN1759190A/zh active Pending
- 2003-11-14 US US10/534,689 patent/US20060149484A1/en not_active Abandoned
- 2003-11-14 JP JP2004553727A patent/JP2006511213A/ja active Pending
- 2003-11-14 RS YUP-2005/0365A patent/RS20050365A/sr unknown
- 2003-11-14 NZ NZ540595A patent/NZ540595A/en unknown
-
2005
- 2005-05-25 ZA ZA200504282A patent/ZA200504282B/en unknown
- 2005-06-13 NO NO20052862A patent/NO20052862L/no not_active Application Discontinuation
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