GEP20074083B - Absolute quantitation of nucleic acids by rt-pcr - Google Patents
Absolute quantitation of nucleic acids by rt-pcrInfo
- Publication number
- GEP20074083B GEP20074083B GEAP20038842A GEAP2003008842A GEP20074083B GE P20074083 B GEP20074083 B GE P20074083B GE AP20038842 A GEAP20038842 A GE AP20038842A GE AP2003008842 A GEAP2003008842 A GE AP2003008842A GE P20074083 B GEP20074083 B GE P20074083B
- Authority
- GE
- Georgia
- Prior art keywords
- crna
- pcr
- absolute quantitation
- nucleic acids
- amplicon
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 238000000034 method Methods 0.000 abstract 3
- 108091093088 Amplicon Proteins 0.000 abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- 241000182988 Assa Species 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000003757 reverse transcription PCR Methods 0.000 abstract 1
- 230000002194 synthesizing effect Effects 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/294,781 US20040096829A1 (en) | 2002-11-14 | 2002-11-14 | Absolute quantitation of nucleic acids by RT-PCR |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GEP20074083B true GEP20074083B (en) | 2007-04-10 |
Family
ID=32297041
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GEAP20038842A GEP20074083B (en) | 2002-11-14 | 2003-11-14 | Absolute quantitation of nucleic acids by rt-pcr |
Country Status (18)
| Country | Link |
|---|---|
| US (2) | US20040096829A1 (enExample) |
| EP (1) | EP1578378A4 (enExample) |
| JP (1) | JP2006511213A (enExample) |
| KR (1) | KR20050074620A (enExample) |
| CN (1) | CN1759190A (enExample) |
| AU (1) | AU2003297278A1 (enExample) |
| BR (1) | BR0316336A (enExample) |
| CA (1) | CA2505992A1 (enExample) |
| EA (1) | EA008742B1 (enExample) |
| GE (1) | GEP20074083B (enExample) |
| IS (1) | IS7853A (enExample) |
| MX (1) | MXPA05005168A (enExample) |
| NO (1) | NO20052862L (enExample) |
| NZ (1) | NZ540595A (enExample) |
| PL (1) | PL377027A1 (enExample) |
| RS (1) | RS20050365A (enExample) |
| WO (1) | WO2004045522A2 (enExample) |
| ZA (1) | ZA200504282B (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007059423A2 (en) * | 2005-11-14 | 2007-05-24 | Siemens Healthcare Diagnostics Inc. | Planar waveguide detection chips and chambers for performing multiplex pcr assays |
| CN100582241C (zh) * | 2007-10-18 | 2010-01-20 | 昆明云大生化科技有限责任公司 | 一种可制作标准曲线的临床定量检测基因芯片的制备方法 |
| US11091803B2 (en) * | 2014-04-14 | 2021-08-17 | W2 Biosolutions, Llc | Nucleic acid quantification method |
| TW201703406A (zh) * | 2015-04-14 | 2017-01-16 | 電源整合有限責任公司 | 切換裝置及功率模組 |
| CN109988846B (zh) * | 2019-03-11 | 2023-05-26 | 浙江省淡水水产研究所 | 一种适于红螯螯虾性腺组织mRNA石蜡切片原位杂交的方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1340807C (en) * | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| US5219727A (en) * | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
| US5543509A (en) * | 1992-08-14 | 1996-08-06 | The United States Of America As Represented By The Department Of Health And Human Services | Method for quantifying laminin and β-actin messenger RNA |
| ZA936015B (en) * | 1992-08-24 | 1994-03-10 | Akzo Nv | Elimination of false negatives in nuleic acid detection. |
| US6093542A (en) * | 1998-01-09 | 2000-07-25 | Akzo Nobel N.V. | Isothermal transcription based amplification assay for the detection and quantitation of macrophage derived chemokine RNA |
| US6365346B1 (en) * | 1998-02-18 | 2002-04-02 | Dade Behring Inc. | Quantitative determination of nucleic acid amplification products |
| EP1055734B1 (en) * | 1999-05-24 | 2004-10-13 | Tosoh Corporation | Method for assaying ribonucleic acid |
| AU1601801A (en) * | 1999-11-16 | 2001-05-30 | Eric M. Eastman | Methods of preparing crna |
-
2002
- 2002-11-14 US US10/294,781 patent/US20040096829A1/en not_active Abandoned
-
2003
- 2003-05-20 IS IS7853A patent/IS7853A/is unknown
- 2003-11-14 MX MXPA05005168A patent/MXPA05005168A/es unknown
- 2003-11-14 US US10/534,689 patent/US20060149484A1/en not_active Abandoned
- 2003-11-14 GE GEAP20038842A patent/GEP20074083B/en unknown
- 2003-11-14 JP JP2004553727A patent/JP2006511213A/ja active Pending
- 2003-11-14 CN CNA2003801075043A patent/CN1759190A/zh active Pending
- 2003-11-14 CA CA002505992A patent/CA2505992A1/en not_active Abandoned
- 2003-11-14 EA EA200500818A patent/EA008742B1/ru not_active IP Right Cessation
- 2003-11-14 EP EP03811624A patent/EP1578378A4/en not_active Withdrawn
- 2003-11-14 AU AU2003297278A patent/AU2003297278A1/en not_active Abandoned
- 2003-11-14 WO PCT/US2003/036522 patent/WO2004045522A2/en not_active Ceased
- 2003-11-14 KR KR1020057008627A patent/KR20050074620A/ko not_active Withdrawn
- 2003-11-14 BR BR0316336-9A patent/BR0316336A/pt not_active IP Right Cessation
- 2003-11-14 PL PL377027A patent/PL377027A1/pl not_active Application Discontinuation
- 2003-11-14 NZ NZ540595A patent/NZ540595A/en unknown
- 2003-11-14 RS YUP-2005/0365A patent/RS20050365A/sr unknown
-
2005
- 2005-05-25 ZA ZA200504282A patent/ZA200504282B/en unknown
- 2005-06-13 NO NO20052862A patent/NO20052862L/no not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| CA2505992A1 (en) | 2004-06-03 |
| ZA200504282B (en) | 2006-08-30 |
| KR20050074620A (ko) | 2005-07-18 |
| WO2004045522A2 (en) | 2004-06-03 |
| PL377027A1 (pl) | 2006-01-23 |
| JP2006511213A (ja) | 2006-04-06 |
| EP1578378A4 (en) | 2007-09-05 |
| CN1759190A (zh) | 2006-04-12 |
| NZ540595A (en) | 2006-12-22 |
| NO20052862D0 (no) | 2005-06-13 |
| AU2003297278A1 (en) | 2004-06-15 |
| IS7853A (is) | 2003-05-20 |
| BR0316336A (pt) | 2005-09-27 |
| WO2004045522A3 (en) | 2005-12-08 |
| US20040096829A1 (en) | 2004-05-20 |
| US20060149484A1 (en) | 2006-07-06 |
| NO20052862L (no) | 2005-08-15 |
| EA008742B1 (ru) | 2007-08-31 |
| RS20050365A (sr) | 2007-08-03 |
| MXPA05005168A (es) | 2005-11-17 |
| EP1578378A2 (en) | 2005-09-28 |
| EA200500818A1 (ru) | 2006-02-24 |
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