CN1759190A - 通过rt－pcr对核酸的绝对定量 - Google Patents

通过rt－pcr对核酸的绝对定量 Download PDF

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Publication number
CN1759190A
CN1759190A CNA2003801075043A CN200380107504A CN1759190A CN 1759190 A CN1759190 A CN 1759190A CN A2003801075043 A CNA2003801075043 A CN A2003801075043A CN 200380107504 A CN200380107504 A CN 200380107504A CN 1759190 A CN1759190 A CN 1759190A
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CN
China
Prior art keywords
amplicon
crna
pcr
nucleotides
synthetic oligonucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CNA2003801075043A
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English (en)
Chinese (zh)
Inventor
诺曼德·E·阿莱尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biogen MA Inc
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Biogen Idec MA Inc
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Filing date
Publication date
Application filed by Biogen Idec MA Inc filed Critical Biogen Idec MA Inc
Publication of CN1759190A publication Critical patent/CN1759190A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CNA2003801075043A 2002-11-14 2003-11-14 通过rt－pcr对核酸的绝对定量 Pending CN1759190A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/294,781 US20040096829A1 (en) 2002-11-14 2002-11-14 Absolute quantitation of nucleic acids by RT-PCR
US10/294,781 2002-11-14

Publications (1)

Publication Number Publication Date
CN1759190A true CN1759190A (zh) 2006-04-12

Family

ID=32297041

Family Applications (1)

Application Number Title Priority Date Filing Date
CNA2003801075043A Pending CN1759190A (zh) 2002-11-14 2003-11-14 通过rt－pcr对核酸的绝对定量

Country Status (18)

Country Link
US (2) US20040096829A1 (enExample)
EP (1) EP1578378A4 (enExample)
JP (1) JP2006511213A (enExample)
KR (1) KR20050074620A (enExample)
CN (1) CN1759190A (enExample)
AU (1) AU2003297278A1 (enExample)
BR (1) BR0316336A (enExample)
CA (1) CA2505992A1 (enExample)
EA (1) EA008742B1 (enExample)
GE (1) GEP20074083B (enExample)
IS (1) IS7853A (enExample)
MX (1) MXPA05005168A (enExample)
NO (1) NO20052862L (enExample)
NZ (1) NZ540595A (enExample)
PL (1) PL377027A1 (enExample)
RS (1) RS20050365A (enExample)
WO (1) WO2004045522A2 (enExample)
ZA (1) ZA200504282B (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100582241C (zh) * 2007-10-18 2010-01-20 昆明云大生化科技有限责任公司 一种可制作标准曲线的临床定量检测基因芯片的制备方法
CN109988846A (zh) * 2019-03-11 2019-07-09 浙江省淡水水产研究所 一种适于红螯螯虾性腺组织mRNA石蜡切片原位杂交的方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1949152A4 (en) * 2005-11-14 2014-02-05 Bayer Ip Gmbh Planar waveguide detection chips and chambers for performing multiplex pcr assays
US11091803B2 (en) * 2014-04-14 2021-08-17 W2 Biosolutions, Llc Nucleic acid quantification method
TW201703406A (zh) * 2015-04-14 2017-01-16 電源整合有限責任公司 切換裝置及功率模組

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5219727A (en) * 1989-08-21 1993-06-15 Hoffmann-Laroche Inc. Quantitation of nucleic acids using the polymerase chain reaction
US5543509A (en) * 1992-08-14 1996-08-06 The United States Of America As Represented By The Department Of Health And Human Services Method for quantifying laminin and β-actin messenger RNA
ZA936015B (en) * 1992-08-24 1994-03-10 Akzo Nv Elimination of false negatives in nuleic acid detection.
US6093542A (en) * 1998-01-09 2000-07-25 Akzo Nobel N.V. Isothermal transcription based amplification assay for the detection and quantitation of macrophage derived chemokine RNA
US6365346B1 (en) * 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
US6541205B1 (en) * 1999-05-24 2003-04-01 Tosoh Corporation Method for assaying nucleic acid
AU1601801A (en) * 1999-11-16 2001-05-30 Eric M. Eastman Methods of preparing crna

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100582241C (zh) * 2007-10-18 2010-01-20 昆明云大生化科技有限责任公司 一种可制作标准曲线的临床定量检测基因芯片的制备方法
CN109988846A (zh) * 2019-03-11 2019-07-09 浙江省淡水水产研究所 一种适于红螯螯虾性腺组织mRNA石蜡切片原位杂交的方法
CN109988846B (zh) * 2019-03-11 2023-05-26 浙江省淡水水产研究所 一种适于红螯螯虾性腺组织mRNA石蜡切片原位杂交的方法

Also Published As

Publication number Publication date
EP1578378A4 (en) 2007-09-05
WO2004045522A3 (en) 2005-12-08
PL377027A1 (pl) 2006-01-23
CA2505992A1 (en) 2004-06-03
IS7853A (is) 2003-05-20
JP2006511213A (ja) 2006-04-06
EA200500818A1 (ru) 2006-02-24
EA008742B1 (ru) 2007-08-31
US20060149484A1 (en) 2006-07-06
NO20052862L (no) 2005-08-15
NZ540595A (en) 2006-12-22
ZA200504282B (en) 2006-08-30
RS20050365A (sr) 2007-08-03
NO20052862D0 (no) 2005-06-13
US20040096829A1 (en) 2004-05-20
EP1578378A2 (en) 2005-09-28
GEP20074083B (en) 2007-04-10
KR20050074620A (ko) 2005-07-18
BR0316336A (pt) 2005-09-27
WO2004045522A2 (en) 2004-06-03
AU2003297278A1 (en) 2004-06-15
MXPA05005168A (es) 2005-11-17

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