JP2006510348A - 蛍光消光脱離基を用いる化学ライゲーションの検出 - Google Patents

蛍光消光脱離基を用いる化学ライゲーションの検出 Download PDF

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JP2006510348A
JP2006510348A JP2004523488A JP2004523488A JP2006510348A JP 2006510348 A JP2006510348 A JP 2006510348A JP 2004523488 A JP2004523488 A JP 2004523488A JP 2004523488 A JP2004523488 A JP 2004523488A JP 2006510348 A JP2006510348 A JP 2006510348A
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nucleic acid
group
acid probe
fluorescence
phosphor
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JP2006510348A5 (enExample
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エリック・ティ・クール
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Leland Stanford Junior University
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Leland Stanford Junior University
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2004523488A 2002-07-18 2003-07-17 蛍光消光脱離基を用いる化学ライゲーションの検出 Pending JP2006510348A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39677402P 2002-07-18 2002-07-18
PCT/US2003/022320 WO2004010101A2 (en) 2002-07-18 2003-07-17 Detection of chemical ligation using fluorescence quenching leaving groups

Publications (2)

Publication Number Publication Date
JP2006510348A true JP2006510348A (ja) 2006-03-30
JP2006510348A5 JP2006510348A5 (enExample) 2008-08-14

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Family Applications (1)

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JP2004523488A Pending JP2006510348A (ja) 2002-07-18 2003-07-17 蛍光消光脱離基を用いる化学ライゲーションの検出

Country Status (6)

Country Link
US (1) US7749699B2 (enExample)
EP (1) EP1543156A4 (enExample)
JP (1) JP2006510348A (enExample)
AU (1) AU2003256587A1 (enExample)
CA (1) CA2492048A1 (enExample)
WO (1) WO2004010101A2 (enExample)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7033753B1 (en) 1999-01-15 2006-04-25 University Of Rochester Compositions and methods for nonenzymatic ligation of oligonucleotides and detection of genetic polymorphisms
US7745614B2 (en) 2004-09-03 2010-06-29 The Board Of Trustees Of The Leland Stanford Junior University Universal linker compositions for the release or transfer of chemical agents from a polynucleotide
CA2610027A1 (en) * 2005-05-26 2006-11-30 Ensemble Discovery Corporation Biodetection by nucleic acid-templated chemistry
EP2677039B8 (en) * 2006-05-10 2022-10-05 DxTerity Diagnostics Incorporated Detection of nucleic acid targets using chemically reactive oligonucleotide probes
JP2008259453A (ja) * 2007-04-12 2008-10-30 Toyobo Co Ltd 核酸の検出方法
KR102231531B1 (ko) 2009-02-13 2021-03-23 엑스-켐, 인크. Ｄｎａ―코딩된 라이브러리의 생성 및 스크리닝 방법
DK2414544T3 (da) 2009-04-01 2014-06-16 Dxterity Diagnostics Inc Probe-amplifikations-afhængig kemisk ligering
WO2012077819A1 (ja) 2010-12-10 2012-06-14 国立大学法人 東京工業大学 標的核酸の検出方法及びキット
WO2012142003A2 (en) 2011-04-15 2012-10-18 Life Technologies Corporation Chemical ligation
CN106434871B (zh) 2011-05-17 2020-08-18 德克斯特里蒂诊断公司 用于检测目标核酸的方法与组合物
ES2675111T3 (es) 2011-09-07 2018-07-06 X-Chem, Inc. Métodos para etiquetar bibliotecas con codificación de ADN
CN104685115A (zh) 2012-07-13 2015-06-03 X-化学有限公司 具有聚合酶不可读的编码性寡核苷酸连接的dna编码文库
MX371428B (es) 2013-08-19 2020-01-30 Singular Bio Inc Ensayos para la deteccion de una sola molecula y uso de los mismos.
JP6739339B2 (ja) 2014-01-15 2020-08-12 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ カバーされた配列変換ｄｎａおよび検出方法
EP3154696B1 (en) 2014-06-10 2020-04-08 DxTerity Diagnostics Incorporated Devices and methods for collecting and stabilizing biological samples
JP6773651B2 (ja) * 2014-10-14 2020-10-21 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ ポリｄｎａスペーサー配列を有する配列変換およびシグナル増幅ｄｎａならびにそれらを用いた検出方法
US10604790B2 (en) 2014-12-24 2020-03-31 Abbott Laboratories Sequence conversion and signal amplifier DNA cascade reactions and detection methods using same
AU2016219943A1 (en) 2015-02-18 2017-10-12 Singular Bio, Inc. Assays for single molecule detection and use thereof
EP3350350B1 (en) * 2015-11-19 2021-04-14 Pacific Biosciences of California, Inc. Compounds and systems for improving signal detection

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5476925A (en) 1993-02-01 1995-12-19 Northwestern University Oligodeoxyribonucleotides including 3'-aminonucleoside-phosphoramidate linkages and terminal 3'-amino groups
WO1994024143A1 (en) 1993-04-12 1994-10-27 Northwestern University Method of forming oligonucleotides
US5681943A (en) 1993-04-12 1997-10-28 Northwestern University Method for covalently linking adjacent oligonucleotides
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
EP0826066B1 (en) * 1995-05-05 2000-09-13 The Perkin-Elmer Corporation Methods and reagents for combined pcr amplification and hybridization probing assay
US6080868A (en) * 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
US5952202A (en) * 1998-03-26 1999-09-14 The Perkin Elmer Corporation Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification

Also Published As

Publication number Publication date
WO2004010101A3 (en) 2004-09-02
CA2492048A1 (en) 2004-01-29
US7749699B2 (en) 2010-07-06
AU2003256587A8 (en) 2004-02-09
AU2003256587A1 (en) 2004-02-09
EP1543156A2 (en) 2005-06-22
WO2004010101A2 (en) 2004-01-29
EP1543156A4 (en) 2005-12-21
US20040259102A1 (en) 2004-12-23

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