JP2005536983A - 核酸におけるハイブリダイゼーション事象を検出するための方法 - Google Patents

核酸におけるハイブリダイゼーション事象を検出するための方法 Download PDF

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JP2005536983A
JP2005536983A JP2003547646A JP2003547646A JP2005536983A JP 2005536983 A JP2005536983 A JP 2005536983A JP 2003547646 A JP2003547646 A JP 2003547646A JP 2003547646 A JP2003547646 A JP 2003547646A JP 2005536983 A JP2005536983 A JP 2005536983A
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nucleic acid
probe
probe nucleic
hybridization
dna
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JP2005536983A5 (enExample
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チャーレ，クリストフ
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フォーカスゲノミクス ゲーエムベーハー
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2003547646A 2001-11-29 2002-11-25 核酸におけるハイブリダイゼーション事象を検出するための方法 Ceased JP2005536983A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10158516A DE10158516A1 (de) 2001-11-29 2001-11-29 Verfahren zum Nachweis von Hybridisierungsereignissen in Nukleinsäuren
PCT/EP2002/013215 WO2003046215A1 (de) 2001-11-29 2002-11-25 Verfahren zum nachweis von hybridisierungsereignissen in nukleinsäuren

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JP2005536983A true JP2005536983A (ja) 2005-12-08
JP2005536983A5 JP2005536983A5 (enExample) 2006-01-26

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JP2003547646A Ceased JP2005536983A (ja) 2001-11-29 2002-11-25 核酸におけるハイブリダイゼーション事象を検出するための方法

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US (1) US20060057569A1 (enExample)
EP (1) EP1453975B1 (enExample)
JP (1) JP2005536983A (enExample)
CN (1) CN1610757A (enExample)
AT (1) ATE335853T1 (enExample)
AU (1) AU2002352121A1 (enExample)
CA (1) CA2468411A1 (enExample)
DE (2) DE10158516A1 (enExample)
WO (1) WO2003046215A1 (enExample)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018518967A (ja) * 2015-06-24 2018-07-19 デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド ヌクレアーゼを使用する野生型ｄｎａの選択的分解および突然変異体対立遺伝子の濃縮
JP2019154396A (ja) * 2018-03-16 2019-09-19 株式会社リコー 核酸検出方法、並びに、それに用いる標的核酸検出用プローブ、及び標的核酸検出用デバイス
US11130992B2 (en) 2011-03-31 2021-09-28 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable multiplex COLD-PCR
US11174510B2 (en) 2010-03-08 2021-11-16 Dana-Farber Cancer Institute, Inc. Full COLD-PCR enrichment with reference blocking sequence
US11371090B2 (en) 2016-12-12 2022-06-28 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010144150A2 (en) 2009-06-12 2010-12-16 Pacific Biosciences Of California, Inc. Real-time analytical methods and systems
EP3491147B1 (en) * 2016-08-01 2020-06-17 H. Hoffnabb-La Roche Ag Methods for removal of adaptor dimers from nucleic acid sequencing preparations
JP7035972B2 (ja) * 2018-11-09 2022-03-15 横河電機株式会社 核酸配列計測用デバイス
US20220372560A1 (en) * 2019-10-30 2022-11-24 Takeda Pharmaceutical Company Limited Methods for detecting oligonucleotides
CN114280128B (zh) * 2021-12-24 2022-11-18 清华大学 双标记gFET的制备及其在miRNA检测中的应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5543292A (en) * 1992-06-16 1996-08-06 Hitachi, Ltd. Process for the measurement of nucleic acids
US5858659A (en) * 1995-11-29 1999-01-12 Affymetrix, Inc. Polymorphism detection
CA2170264A1 (en) * 1993-09-10 1995-03-16 Michael W. Konrad Optical detection of position of oligonucleotides on large dna molecules
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
AU2001292794A1 (en) * 2000-09-19 2002-04-02 Atom Sciences, Inc. Detection of unlabeled hybridized dna and rna using restriction enzyme digestion

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11174510B2 (en) 2010-03-08 2021-11-16 Dana-Farber Cancer Institute, Inc. Full COLD-PCR enrichment with reference blocking sequence
US11130992B2 (en) 2011-03-31 2021-09-28 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable multiplex COLD-PCR
JP2018518967A (ja) * 2015-06-24 2018-07-19 デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド ヌクレアーゼを使用する野生型ｄｎａの選択的分解および突然変異体対立遺伝子の濃縮
US11725230B2 (en) 2015-06-24 2023-08-15 Dana-Farber Cancer Institute, Inc. Selective degradation of wild-type DNA and enrichment of mutant alleles using nuclease
US11371090B2 (en) 2016-12-12 2022-06-28 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection
JP2019154396A (ja) * 2018-03-16 2019-09-19 株式会社リコー 核酸検出方法、並びに、それに用いる標的核酸検出用プローブ、及び標的核酸検出用デバイス

Also Published As

Publication number Publication date
DE10158516A1 (de) 2003-06-12
CN1610757A (zh) 2005-04-27
DE50207820D1 (de) 2006-09-21
AU2002352121A1 (en) 2003-06-10
US20060057569A1 (en) 2006-03-16
WO2003046215A1 (de) 2003-06-05
ATE335853T1 (de) 2006-09-15
EP1453975B1 (de) 2006-08-09
CA2468411A1 (en) 2003-06-05
EP1453975A1 (de) 2004-09-08

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