CA2468411A1 - Method for detecting hybridization events in nucleic acids - Google Patents
Method for detecting hybridization events in nucleic acids Download PDFInfo
- Publication number
- CA2468411A1 CA2468411A1 CA002468411A CA2468411A CA2468411A1 CA 2468411 A1 CA2468411 A1 CA 2468411A1 CA 002468411 A CA002468411 A CA 002468411A CA 2468411 A CA2468411 A CA 2468411A CA 2468411 A1 CA2468411 A1 CA 2468411A1
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acid
- nucleic acids
- probe
- probe nucleic
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 198
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 182
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 182
- 238000009396 hybridization Methods 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 40
- 239000000523 sample Substances 0.000 claims abstract description 163
- 108020004414 DNA Proteins 0.000 claims abstract description 72
- 239000007790 solid phase Substances 0.000 claims abstract description 22
- 238000003776 cleavage reaction Methods 0.000 claims abstract description 16
- 230000007017 scission Effects 0.000 claims abstract description 16
- 230000000295 complement effect Effects 0.000 claims abstract description 14
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 13
- 108091008146 restriction endonucleases Proteins 0.000 claims description 24
- 230000027455 binding Effects 0.000 claims description 11
- 101710163270 Nuclease Proteins 0.000 abstract description 21
- 239000003550 marker Substances 0.000 abstract 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 22
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 16
- 230000015572 biosynthetic process Effects 0.000 description 13
- 239000000243 solution Substances 0.000 description 13
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 11
- 239000007787 solid Substances 0.000 description 11
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 10
- 229920001213 Polysorbate 20 Polymers 0.000 description 10
- 238000010521 absorption reaction Methods 0.000 description 10
- 238000005516 engineering process Methods 0.000 description 10
- 239000011159 matrix material Substances 0.000 description 10
- 238000002493 microarray Methods 0.000 description 10
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 10
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 10
- 239000002773 nucleotide Substances 0.000 description 9
- 238000001514 detection method Methods 0.000 description 8
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 8
- 238000002372 labelling Methods 0.000 description 8
- 239000011780 sodium chloride Substances 0.000 description 8
- 238000003786 synthesis reaction Methods 0.000 description 8
- 108091034117 Oligonucleotide Proteins 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- 239000011534 wash buffer Substances 0.000 description 7
- 238000005406 washing Methods 0.000 description 7
- -1 Cy5 Chemical compound 0.000 description 6
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 6
- 239000002253 acid Substances 0.000 description 6
- 238000003491 array Methods 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 108020004999 messenger RNA Proteins 0.000 description 6
- 125000003729 nucleotide group Chemical group 0.000 description 6
- 239000011535 reaction buffer Substances 0.000 description 6
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 5
- 150000007513 acids Chemical class 0.000 description 5
- 229960002685 biotin Drugs 0.000 description 5
- 235000020958 biotin Nutrition 0.000 description 5
- 239000011616 biotin Substances 0.000 description 5
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 5
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 5
- 230000005284 excitation Effects 0.000 description 5
- 238000002844 melting Methods 0.000 description 5
- 230000008018 melting Effects 0.000 description 5
- 239000001226 triphosphate Substances 0.000 description 5
- 235000011178 triphosphate Nutrition 0.000 description 5
- 241000972773 Aulopiformes Species 0.000 description 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 4
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 4
- 108010042407 Endonucleases Proteins 0.000 description 4
- 102000004533 Endonucleases Human genes 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 239000007983 Tris buffer Substances 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 238000010369 molecular cloning Methods 0.000 description 4
- 235000019515 salmon Nutrition 0.000 description 4
- 125000006850 spacer group Chemical group 0.000 description 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 4
- 101001007348 Arachis hypogaea Galactose-binding lectin Proteins 0.000 description 3
- 239000003298 DNA probe Substances 0.000 description 3
- 108091027305 Heteroduplex Proteins 0.000 description 3
- 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 3
- 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 238000010195 expression analysis Methods 0.000 description 3
- 238000003500 gene array Methods 0.000 description 3
- 239000011859 microparticle Substances 0.000 description 3
- 239000002105 nanoparticle Substances 0.000 description 3
- 238000007639 printing Methods 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 239000012089 stop solution Substances 0.000 description 3
- 230000002194 synthesizing effect Effects 0.000 description 3
- 238000013518 transcription Methods 0.000 description 3
- 230000035897 transcription Effects 0.000 description 3
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 238000000018 DNA microarray Methods 0.000 description 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
- 102100031780 Endonuclease Human genes 0.000 description 2
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 2
- 108010090804 Streptavidin Proteins 0.000 description 2
- 239000007984 Tris EDTA buffer Substances 0.000 description 2
- 125000003277 amino group Chemical group 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000008033 biological extinction Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 238000003759 clinical diagnosis Methods 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 125000000524 functional group Chemical group 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000000329 molecular dynamics simulation Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 description 1
- 102000013563 Acid Phosphatase Human genes 0.000 description 1
- 108010051457 Acid Phosphatase Proteins 0.000 description 1
- 108010017826 DNA Polymerase I Proteins 0.000 description 1
- 102000004594 DNA Polymerase I Human genes 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 108060002716 Exonuclease Proteins 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 241000192041 Micrococcus Species 0.000 description 1
- 108010086093 Mung Bean Nuclease Proteins 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 101710149004 Nuclease P1 Proteins 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 108020002230 Pancreatic Ribonuclease Proteins 0.000 description 1
- 102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 108010004729 Phycoerythrin Proteins 0.000 description 1
- 108010065868 RNA polymerase SP6 Proteins 0.000 description 1
- 108091028733 RNTP Proteins 0.000 description 1
- 108010046983 Ribonuclease T1 Proteins 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 101710137500 T7 RNA polymerase Proteins 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- 108010028263 bacteriophage T3 RNA polymerase Proteins 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001218 confocal laser scanning microscopy Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000000295 emission spectrum Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000695 excitation spectrum Methods 0.000 description 1
- 102000013165 exonuclease Human genes 0.000 description 1
- 108010092809 exonuclease Bal 31 Proteins 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000001215 fluorescent labelling Methods 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 230000000155 isotopic effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- WLHQHAUOOXYABV-UHFFFAOYSA-N lornoxicam Chemical compound OC=1C=2SC(Cl)=CC=2S(=O)(=O)N(C)C=1C(=O)NC1=CC=CC=N1 WLHQHAUOOXYABV-UHFFFAOYSA-N 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 229940046166 oligodeoxynucleotide Drugs 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 238000002515 oligonucleotide synthesis Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 108010068698 spleen exonuclease Proteins 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 230000004304 visual acuity Effects 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10158516.0 | 2001-11-29 | ||
| DE10158516A DE10158516A1 (de) | 2001-11-29 | 2001-11-29 | Verfahren zum Nachweis von Hybridisierungsereignissen in Nukleinsäuren |
| PCT/EP2002/013215 WO2003046215A1 (de) | 2001-11-29 | 2002-11-25 | Verfahren zum nachweis von hybridisierungsereignissen in nukleinsäuren |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2468411A1 true CA2468411A1 (en) | 2003-06-05 |
Family
ID=7707346
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002468411A Abandoned CA2468411A1 (en) | 2001-11-29 | 2002-11-25 | Method for detecting hybridization events in nucleic acids |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20060057569A1 (enExample) |
| EP (1) | EP1453975B1 (enExample) |
| JP (1) | JP2005536983A (enExample) |
| CN (1) | CN1610757A (enExample) |
| AT (1) | ATE335853T1 (enExample) |
| AU (1) | AU2002352121A1 (enExample) |
| CA (1) | CA2468411A1 (enExample) |
| DE (2) | DE10158516A1 (enExample) |
| WO (1) | WO2003046215A1 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9063156B2 (en) * | 2009-06-12 | 2015-06-23 | Pacific Biosciences Of California, Inc. | Real-time analytical methods and systems |
| EP2545189B1 (en) | 2010-03-08 | 2018-01-10 | Dana-Farber Cancer Institute, Inc. | Full cold-pcr enrichment with reference blocking sequence |
| US11130992B2 (en) | 2011-03-31 | 2021-09-28 | Dana-Farber Cancer Institute, Inc. | Methods and compositions to enable multiplex COLD-PCR |
| JP6941568B2 (ja) * | 2015-06-24 | 2021-09-29 | デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド | ヌクレアーゼを使用する野生型ｄｎａの選択的分解および突然変異体対立遺伝子の濃縮 |
| JP7096812B2 (ja) * | 2016-08-01 | 2022-07-06 | エフ．ホフマン－ラ ロシュ アーゲー | 核酸配列決定調製物からアダプター二量体を除去する方法 |
| WO2018111835A1 (en) | 2016-12-12 | 2018-06-21 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection |
| JP2019154396A (ja) * | 2018-03-16 | 2019-09-19 | 株式会社リコー | 核酸検出方法、並びに、それに用いる標的核酸検出用プローブ、及び標的核酸検出用デバイス |
| JP7035972B2 (ja) * | 2018-11-09 | 2022-03-15 | 横河電機株式会社 | 核酸配列計測用デバイス |
| WO2021087164A1 (en) * | 2019-10-30 | 2021-05-06 | Shire Human Genetic Therapies, Inc. | Methods for detecting oligonucleotides |
| CN114280128B (zh) * | 2021-12-24 | 2022-11-18 | 清华大学 | 双标记gFET的制备及其在miRNA检测中的应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5543292A (en) * | 1992-06-16 | 1996-08-06 | Hitachi, Ltd. | Process for the measurement of nucleic acids |
| US5858659A (en) * | 1995-11-29 | 1999-01-12 | Affymetrix, Inc. | Polymorphism detection |
| CA2170264A1 (en) * | 1993-09-10 | 1995-03-16 | Michael W. Konrad | Optical detection of position of oligonucleotides on large dna molecules |
| US5925517A (en) * | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| WO2002024955A2 (en) * | 2000-09-19 | 2002-03-28 | Atom Sciences, Inc. | Detection of unlabeled hybridized dna and rna using restriction enzyme digestion |
-
2001
- 2001-11-29 DE DE10158516A patent/DE10158516A1/de not_active Withdrawn
-
2002
- 2002-11-25 AU AU2002352121A patent/AU2002352121A1/en not_active Abandoned
- 2002-11-25 EP EP02787794A patent/EP1453975B1/de not_active Expired - Lifetime
- 2002-11-25 AT AT02787794T patent/ATE335853T1/de not_active IP Right Cessation
- 2002-11-25 US US10/497,130 patent/US20060057569A1/en not_active Abandoned
- 2002-11-25 CA CA002468411A patent/CA2468411A1/en not_active Abandoned
- 2002-11-25 DE DE50207820T patent/DE50207820D1/de not_active Expired - Fee Related
- 2002-11-25 JP JP2003547646A patent/JP2005536983A/ja not_active Ceased
- 2002-11-25 WO PCT/EP2002/013215 patent/WO2003046215A1/de not_active Ceased
- 2002-11-25 CN CNA028265599A patent/CN1610757A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20060057569A1 (en) | 2006-03-16 |
| CN1610757A (zh) | 2005-04-27 |
| WO2003046215A1 (de) | 2003-06-05 |
| DE10158516A1 (de) | 2003-06-12 |
| EP1453975A1 (de) | 2004-09-08 |
| ATE335853T1 (de) | 2006-09-15 |
| JP2005536983A (ja) | 2005-12-08 |
| EP1453975B1 (de) | 2006-08-09 |
| DE50207820D1 (de) | 2006-09-21 |
| AU2002352121A1 (en) | 2003-06-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Fang et al. | Peer reviewed: molecular beacons: novel fluorescent probes | |
| US6579680B2 (en) | Method for label-free detection of hybridized DNA targets | |
| Tan et al. | Molecular beacons: a novel DNA probe for nucleic acid and protein studies | |
| EP2711434B1 (en) | Multiplex detection of nucleic acids | |
| EP1319179B1 (en) | Methods for detecting and assaying nucleic acid sequences | |
| US20120172246A1 (en) | Detection of Nucleic Acids | |
| CA2378569A1 (en) | Method for relative quantization of methylation of cytosin-type bases in dna samples | |
| CN110050072B (zh) | 用于标记寡核苷酸探针的方法 | |
| WO2003054223A2 (en) | Tripartite molecular beacons | |
| US6316608B1 (en) | Combined polynucleotide sequence as discrete assay endpoints | |
| CA2400680A1 (en) | Methods for assay and detection on a microarray | |
| WO2004097048A1 (en) | Process for assay of nucleic acid by competitive hybridization using a dna microarray | |
| CA2468411A1 (en) | Method for detecting hybridization events in nucleic acids | |
| US20070172841A1 (en) | Probe/target stabilization with add-in oligo | |
| US20040009514A1 (en) | Assembly for label-free detection of hybridized nucleic targets | |
| CA2389268A1 (en) | Normalization controls and duplex probes for quantitative hybridization reactions | |
| US20120141986A1 (en) | Multivalent substrate elements for detection of nucleic acid sequences | |
| US7754475B2 (en) | Nucleic acid probes and microarrays for analysis of polynucleotides | |
| US20050074781A1 (en) | Nucleic acid braided J-probes | |
| WO2008048310A2 (en) | Bimolecular constructs | |
| US20090011514A1 (en) | Dna Crosslinking for Primer Extension Assays | |
| WO2005003373A2 (en) | Fluorogenic nucleic acid probes including lna for methods to detect and/or quantify nucleic acid analytes | |
| KR20090116475A (ko) | 마이크로어레이에 고정되어 있는 프로브 핵산 서열의정확도를 분석하는 방법 | |
| Vasantgadkar | A novel method for detecting DNA | |
| Wang et al. | Improvement of Microarray Technologies for Detecting Single Nucleotide Mismatch |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |