JP2005532543A5 - - Google Patents

Info

Publication number

JP2005532543A5

JP2005532543A5 JP2004518895A JP2004518895A JP2005532543A5 JP 2005532543 A5 JP2005532543 A5 JP 2005532543A5 JP 2004518895 A JP2004518895 A JP 2004518895A JP 2004518895 A JP2004518895 A JP 2004518895A JP 2005532543 A5 JP2005532543 A5 JP 2005532543A5

Authority

JP

Japan

Prior art keywords

dihydro

group

dimethyl

dyes

ethyl

Prior art date

2002-07-08

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 238000000034 method Methods 0.000 claims description 15
• 239000007850 fluorescent dye Substances 0.000 claims description 14
• 235000018102 proteins Nutrition 0.000 claims description 14
• 102000004169 proteins and genes Human genes 0.000 claims description 14
• 108090000623 proteins and genes Proteins 0.000 claims description 14
• 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 claims description 5
• 239000000975 dye Substances 0.000 claims 23
• 150000001875 compounds Chemical class 0.000 claims 7
• -1 6-{[2- (2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl) ethyl] amino} -6-oxohexyl Chemical group 0.000 claims 5
• 238000002372 labelling Methods 0.000 claims 5
• 150000003839 salts Chemical class 0.000 claims 5
• UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims 4
• 125000004432 carbon atom Chemical group C* 0.000 claims 4
• 229910052739 hydrogen Inorganic materials 0.000 claims 4
• 239000001257 hydrogen Substances 0.000 claims 4
• 125000005439 maleimidyl group Chemical group C1(C=CC(N1*)=O)=O 0.000 claims 4
• 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims 4
• BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical group [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 claims 4
• 125000000542 sulfonic acid group Chemical group 0.000 claims 4
• PGLTVOMIXTUURA-UHFFFAOYSA-N iodoacetamide Chemical group NC(=O)CI PGLTVOMIXTUURA-UHFFFAOYSA-N 0.000 claims 3
• YZCIACABIXJAFH-UHFFFAOYSA-N CC1(C)C2=CC(S([O-])(=O)=O)=CC=C2N(CC)\C1=C\C=C\C(C(C1=CC=CC=C11)(C)C)=[N+]1CCCCCC(=O)NCCN1C(=O)C=CC1=O Chemical compound CC1(C)C2=CC(S([O-])(=O)=O)=CC=C2N(CC)\C1=C\C=C\C(C(C1=CC=CC=C11)(C)C)=[N+]1CCCCCC(=O)NCCN1C(=O)C=CC1=O YZCIACABIXJAFH-UHFFFAOYSA-N 0.000 claims 2
• FKLJPTJMIBLJAV-UHFFFAOYSA-N Compound IV Chemical compound O1N=C(C)C=C1CCCCCCCOC1=CC=C(C=2OCCN=2)C=C1 FKLJPTJMIBLJAV-UHFFFAOYSA-N 0.000 claims 2
• 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims 2
• 239000007788 liquid Substances 0.000 claims 2
• 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 2
• FCSXRQTZLPYGNV-UHFFFAOYSA-N (2E)-2-[(E)-3-[1-[6-[2-(2,5-dioxopyrrol-1-yl)ethylamino]-6-oxohexyl]-3,3-dimethylindol-1-ium-2-yl]prop-2-enylidene]-3,3-dimethyl-1-propylindole-5-sulfonate Chemical compound CC1(C)C2=CC(S([O-])(=O)=O)=CC=C2N(CCC)\C1=C\C=C\C(C(C1=CC=CC=C11)(C)C)=[N+]1CCCCCC(=O)NCCN1C(=O)C=CC1=O FCSXRQTZLPYGNV-UHFFFAOYSA-N 0.000 claims 1
• HTSGKJQDMSTCGS-UHFFFAOYSA-N 1,4-bis(4-chlorophenyl)-2-(4-methylphenyl)sulfonylbutane-1,4-dione Chemical compound C1=CC(C)=CC=C1S(=O)(=O)C(C(=O)C=1C=CC(Cl)=CC=1)CC(=O)C1=CC=C(Cl)C=C1 HTSGKJQDMSTCGS-UHFFFAOYSA-N 0.000 claims 1
• FCSKOFQQCWLGMV-UHFFFAOYSA-N 5-{5-[2-chloro-4-(4,5-dihydro-1,3-oxazol-2-yl)phenoxy]pentyl}-3-methylisoxazole Chemical compound O1N=C(C)C=C1CCCCCOC1=CC=C(C=2OCCN=2)C=C1Cl FCSKOFQQCWLGMV-UHFFFAOYSA-N 0.000 claims 1
• LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims 1
• CBEPIBCXDUBBDV-UHFFFAOYSA-N CC1(C)C2=CC(S([O-])(=O)=O)=CC=C2N(CC)\C1=C\C=C\C(C(C1=CC=CC=C11)(C)C)=[N+]1CCCCCC(=O)NCCCN1C(=O)C=CC1=O Chemical compound CC1(C)C2=CC(S([O-])(=O)=O)=CC=C2N(CC)\C1=C\C=C\C(C(C1=CC=CC=C11)(C)C)=[N+]1CCCCCC(=O)NCCCN1C(=O)C=CC1=O CBEPIBCXDUBBDV-UHFFFAOYSA-N 0.000 claims 1
• SOPHKGJHIXGYRN-UHFFFAOYSA-N CCCC(N1C2=CC=CC=C2C(C)(C)/C\1=C\C=C\C1=[N+](CCCCCC(NCCCN(C(C=C2)=O)C2=O)=O)C2=CC=CC=C2C1(C)C)S([O-])(=O)=O Chemical compound CCCC(N1C2=CC=CC=C2C(C)(C)/C\1=C\C=C\C1=[N+](CCCCCC(NCCCN(C(C=C2)=O)C2=O)=O)C2=CC=CC=C2C1(C)C)S([O-])(=O)=O SOPHKGJHIXGYRN-UHFFFAOYSA-N 0.000 claims 1
• LQTBNHOBNNVRAN-UHFFFAOYSA-N CCCC(N1C2=CC=CC=C2C(C)(C)/C\1=C\C=C\C1=[N+](CCCCCC(NCCN(C(C=C2)=O)C2=O)=O)C2=CC=CC=C2C1(C)C)S([O-])(=O)=O Chemical compound CCCC(N1C2=CC=CC=C2C(C)(C)/C\1=C\C=C\C1=[N+](CCCCCC(NCCN(C(C=C2)=O)C2=O)=O)C2=CC=CC=C2C1(C)C)S([O-])(=O)=O LQTBNHOBNNVRAN-UHFFFAOYSA-N 0.000 claims 1
• 239000003638 chemical reducing agent Substances 0.000 claims 1
• NLFBCYMMUAKCPC-KQQUZDAGSA-N ethyl (e)-3-[3-amino-2-cyano-1-[(e)-3-ethoxy-3-oxoprop-1-enyl]sulfanyl-3-oxoprop-1-enyl]sulfanylprop-2-enoate Chemical compound CCOC(=O)\C=C\SC(=C(C#N)C(N)=O)S\C=C\C(=O)OCC NLFBCYMMUAKCPC-KQQUZDAGSA-N 0.000 claims 1
• 125000005647 linker group Chemical group 0.000 claims 1
• 239000000203 mixture Substances 0.000 claims 1
• 125000001273 sulfonato group Chemical group [O-]S(*)(=O)=O 0.000 claims 1
• ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 claims 1
• 0 *C1C([N+](*)[O-])=C1 Chemical compound *C1C([N+](*)[O-])=C1 0.000 description 4
• 125000000539 amino acid group Chemical group 0.000 description 2
• 235000001014 amino acid Nutrition 0.000 description 1
• 150000001413 amino acids Chemical class 0.000 description 1
• 238000009738 saturating Methods 0.000 description 1
• 125000003396 thiol group Chemical class [H]S* 0.000 description 1