ATE460670T1 - Reagenzien sowie ein verfahren zur sättigungsmarkierung von proteinen - Google Patents
Reagenzien sowie ein verfahren zur sättigungsmarkierung von proteinenInfo
- Publication number
- ATE460670T1 ATE460670T1 AT02747568T AT02747568T ATE460670T1 AT E460670 T1 ATE460670 T1 AT E460670T1 AT 02747568 T AT02747568 T AT 02747568T AT 02747568 T AT02747568 T AT 02747568T AT E460670 T1 ATE460670 T1 AT E460670T1
- Authority
- AT
- Austria
- Prior art keywords
- protein
- dye
- labeled
- matched
- group
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 7
- 108090000623 proteins and genes Proteins 0.000 title abstract 7
- 238000002372 labelling Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- 235000018102 proteins Nutrition 0.000 abstract 6
- 239000000975 dye Substances 0.000 abstract 4
- 239000007850 fluorescent dye Substances 0.000 abstract 3
- 229910052739 hydrogen Inorganic materials 0.000 abstract 2
- 235000001014 amino acid Nutrition 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- JXLHNMVSKXFWAO-UHFFFAOYSA-N azane;7-fluoro-2,1,3-benzoxadiazole-4-sulfonic acid Chemical group N.OS(=O)(=O)C1=CC=C(F)C2=NON=C12 JXLHNMVSKXFWAO-UHFFFAOYSA-N 0.000 abstract 1
- 125000004432 carbon atom Chemical group C* 0.000 abstract 1
- 235000018417 cysteine Nutrition 0.000 abstract 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 abstract 1
- 239000001257 hydrogen Substances 0.000 abstract 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 abstract 1
- 125000005647 linker group Chemical group 0.000 abstract 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 abstract 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-N sulfonic acid Chemical group OS(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/13—Labelling of peptides
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0033—Blends of pigments; Mixtured crystals; Solid solutions
- C09B67/0034—Mixtures of two or more pigments or dyes of the same type
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6806—Determination of free amino acids
- G01N33/6812—Assays for specific amino acids
- G01N33/6815—Assays for specific amino acids containing sulfur, e.g. cysteine, cystine, methionine, homocysteine
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Urology & Nephrology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Food Science & Technology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Plural Heterocyclic Compounds (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Peptides Or Proteins (AREA)
- Indole Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/GB2002/003142 WO2004005933A1 (en) | 2002-07-08 | 2002-07-08 | Reagents and a method for saturation labelling of proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE460670T1 true ATE460670T1 (de) | 2010-03-15 |
Family
ID=30011660
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02747568T ATE460670T1 (de) | 2002-07-08 | 2002-07-08 | Reagenzien sowie ein verfahren zur sättigungsmarkierung von proteinen |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7511155B2 (de) |
| EP (1) | EP1520176B1 (de) |
| JP (1) | JP4318180B2 (de) |
| CN (1) | CN1307263C (de) |
| AT (1) | ATE460670T1 (de) |
| AU (1) | AU2002317958B2 (de) |
| CA (1) | CA2491692C (de) |
| DE (1) | DE60235652D1 (de) |
| ES (1) | ES2341946T3 (de) |
| WO (1) | WO2004005933A1 (de) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE112005003114T5 (de) * | 2004-12-06 | 2008-05-08 | Katayanagi Institute, Hachioji | Verfahren zum Trennen von Protein, Verfahren zum Färben von Protein und flüssiges Proteinfärbemittel sowie Proteinfärbekit zur Verwendung bei diesen Verfahren |
| US20110186434A1 (en) * | 2008-07-23 | 2011-08-04 | Leibniz Institute of Plant Biochemistry | Qualitative and/or quantitative determination of a proteinaceous molecule in a plurality of samples |
| JP5213967B2 (ja) * | 2008-12-02 | 2013-06-19 | 独立行政法人科学技術振興機構 | 芳香族スルホン酸化合物を用いた新規ＣｌｅａｒＮａｔｉｖｅ電気泳動法 |
| US8729276B2 (en) | 2009-04-17 | 2014-05-20 | Korea Institute Of Science And Technology | Cyanine compound for labeling biomolecule and preparation method thereof |
| SG191371A1 (en) * | 2010-12-28 | 2013-08-30 | Chugai Pharmaceutical Co Ltd | Animal cell culturing method |
| CN103113283A (zh) * | 2013-01-22 | 2013-05-22 | 天津理工大学 | 一种含给电子基团半菁蓝素温敏分子光学探针的制备方法 |
| CN103113282A (zh) * | 2013-01-22 | 2013-05-22 | 天津理工大学 | 一种含吸电子基团半菁蓝素温敏分子光学探针的制备方法 |
| DE102022131450A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131444A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren zum Identifizieren von Analyten in einer Bildfolge |
| DE102022131446A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131445A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131449A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131451A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Bestimmen einer Signal-Zusammensetzung von Signalfolgen einer Bildfolge |
| DE102022131447A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131448A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102023122213A1 (de) * | 2023-08-18 | 2025-02-20 | Carl Zeiss Microscopy Gmbh | Verfahren zum Bereitstellen von Quelldaten zum Trainieren oder Validieren eines Verarbeitungsmodells zum Verarbeiten von Analyt-Bildfolgen |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5268486A (en) * | 1986-04-18 | 1993-12-07 | Carnegie-Mellon Unversity | Method for labeling and detecting materials employing arylsulfonate cyanine dyes |
| US6127134A (en) * | 1995-04-20 | 2000-10-03 | Carnegie Mellon University | Difference gel electrophoresis using matched multiple dyes |
| BR9814816A (pt) * | 1997-10-10 | 2004-06-22 | Cytovia Inc | Novas moléculas repórter fluorescentes e suas aplicações incluindo análises de caspases |
| EP1038938B1 (de) * | 1999-03-25 | 2004-05-26 | Nisshinbo Industries, Inc. | Carbodiimidverbindungen, die eine fluoreszierende Gruppe enthalten, deren Vorstufen und Verfahren zu deren Herstellung |
| US6114350A (en) * | 1999-04-19 | 2000-09-05 | Nen Life Science Products, Inc. | Cyanine dyes and synthesis methods thereof |
| AU2001294859A1 (en) * | 2000-09-29 | 2002-04-08 | Molecular Probes, Inc. | Modified carbocyanine dyes and their conjugates |
| JP2003048879A (ja) * | 2001-05-31 | 2003-02-21 | Nisshinbo Ind Inc | 蛍光性基含有カルボジイミド化合物及び該化合物の製造方法 |
| US8227621B2 (en) * | 2005-06-30 | 2012-07-24 | Li-Cor, Inc. | Cyanine dyes and methods of use |
-
2002
- 2002-07-08 EP EP02747568A patent/EP1520176B1/de not_active Expired - Lifetime
- 2002-07-08 US US10/519,433 patent/US7511155B2/en not_active Expired - Lifetime
- 2002-07-08 AT AT02747568T patent/ATE460670T1/de not_active IP Right Cessation
- 2002-07-08 CA CA2491692A patent/CA2491692C/en not_active Expired - Lifetime
- 2002-07-08 CN CNB02829498XA patent/CN1307263C/zh not_active Expired - Lifetime
- 2002-07-08 AU AU2002317958A patent/AU2002317958B2/en not_active Expired
- 2002-07-08 DE DE60235652T patent/DE60235652D1/de not_active Expired - Lifetime
- 2002-07-08 JP JP2004518895A patent/JP4318180B2/ja not_active Expired - Lifetime
- 2002-07-08 ES ES02747568T patent/ES2341946T3/es not_active Expired - Lifetime
- 2002-07-08 WO PCT/GB2002/003142 patent/WO2004005933A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP1520176A1 (de) | 2005-04-06 |
| DE60235652D1 (de) | 2010-04-22 |
| CN1650168A (zh) | 2005-08-03 |
| AU2002317958A1 (en) | 2004-01-23 |
| CA2491692A1 (en) | 2004-01-15 |
| CN1307263C (zh) | 2007-03-28 |
| US20050233462A1 (en) | 2005-10-20 |
| US7511155B2 (en) | 2009-03-31 |
| ES2341946T3 (es) | 2010-06-30 |
| CA2491692C (en) | 2010-12-07 |
| AU2002317958B2 (en) | 2007-07-12 |
| EP1520176B1 (de) | 2010-03-10 |
| JP4318180B2 (ja) | 2009-08-19 |
| JP2005532543A (ja) | 2005-10-27 |
| WO2004005933A1 (en) | 2004-01-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE460670T1 (de) | Reagenzien sowie ein verfahren zur sättigungsmarkierung von proteinen | |
| DK1141399T3 (da) | Sekventeringsfremgangsmåde under anvendelse af forstörrelsesmærker | |
| DE50012021D1 (de) | Nucleinsauremoleküle mit spezifischer erkennung von nativem prpsc, herstellung und verwendung | |
| CO4810232A1 (es) | Citoquinas de mamiferos y sus secuencias codificadoras | |
| DE69942451D1 (de) | Methode zum schnellen identifizieren von kleinen organischen liganden | |
| NO20001052L (no) | 1C, 15N, 2H merkede proteiner for NMR-strukturbestemmelser og deres fremstilling | |
| ATE541829T1 (de) | Mit stabilen isotopen markierte aminosäure, verfahren zu deren einschleusung in ein zielprotein, verfahren zur nmr-strukturanalyse von protein | |
| IL162568A0 (en) | fluorescent protein from aequorea coerulscens and uses thereof | |
| CU23302A1 (es) | Medio de cultivo selectivo para el aislamiento y detecciã"n de especies del gã0/00nero streptococcus | |
| ATE483161T1 (de) | Zur vereinfachung komplexer peptidgemische geeignete verfahren und kits | |
| ATE532793T1 (de) | Verfahren zur herstellung von molekülen mit verminderter immunogenität | |
| DE69734586D1 (de) | Retinoid-metabolisierendes protein | |
| JP2005189232A (ja) | 複合体混合物中のタンパク質の同定と定量的分析のための選択的ペプチド単離法 | |
| SE0300586D0 (sv) | Diagnosis of autism | |
| EP1437338A4 (de) | Leuchtverbindungen und markierungsreagentien für die diese verwendet werden | |
| NZ596259A (en) | Biologically produced cyclic affinity tags comprising cyclized streptavidin binding sequence | |
| Rohlfing et al. | Evolutionary processes possibly limiting the kinds of amino acids in protein to twenty: a review | |
| ATE187162T1 (de) | Verfahren zur herstellung von alpha,omega- diaminosäuren abgeleiteten harnstoffen | |
| PT95374A (pt) | Processo para a preparacao de anticorpos contra sequencias de aminoacidos altamente conservadas de substancias imunogenicas bem como de meios de ensaio imunologico que contenham esses anticorpos | |
| EP1398378A4 (de) | In neuroblastom isolierte nukleinsäuren | |
| ECSP982602A (es) | Citoquinas de mamiferos, reactivos relacionados | |
| RU94002991A (ru) | Способ идентификации мумиеподобных веществ | |
| Wilchek et al. | A. Zamir, I. Ginzburg, N. Sonenberg, R. Miskin | |
| ATE291641T1 (de) | Verfahren zur erstellung von profilen der differentialen proteinexpression | |
| DE60000965D1 (de) | Pseudopeptid, verfahren zur herstellung, reagenz und verwendungen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |