CN1307263C - 用于饱和标记蛋白质的荧光染料、方法及试剂盒 - Google Patents

用于饱和标记蛋白质的荧光染料、方法及试剂盒 Download PDF

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Publication number
CN1307263C
CN1307263C CNB02829498XA CN02829498A CN1307263C CN 1307263 C CN1307263 C CN 1307263C CN B02829498X A CNB02829498X A CN B02829498XA CN 02829498 A CN02829498 A CN 02829498A CN 1307263 C CN1307263 C CN 1307263C
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CN
China
Prior art keywords
group
dihydro
dimethyl
indoles
dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CNB02829498XA
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English (en)
Chinese (zh)
Other versions
CN1650168A (zh
Inventor
K·威廉斯
T·斯通
A·C·辛蒙兹
A·C·斯维特
S·J·福勒
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions Germany GmbH
Cytiva Europe GmbH
Original Assignee
GE Healthcare UK Ltd
Amersham Biosciences UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare UK Ltd, Amersham Biosciences UK Ltd filed Critical GE Healthcare UK Ltd
Publication of CN1650168A publication Critical patent/CN1650168A/zh
Application granted granted Critical
Publication of CN1307263C publication Critical patent/CN1307263C/zh
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
    • C09B67/0033—Blends of pigments; Mixtured crystals; Solid solutions
    • C09B67/0034—Mixtures of two or more pigments or dyes of the same type
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6806—Determination of free amino acids
    • G01N33/6812—Assays for specific amino acids
    • G01N33/6815—Assays for specific amino acids containing sulfur, e.g. cysteine, cystine, methionine, homocysteine

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Indole Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
CNB02829498XA 2002-07-08 2002-07-08 用于饱和标记蛋白质的荧光染料、方法及试剂盒 Expired - Lifetime CN1307263C (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/GB2002/003142 WO2004005933A1 (en) 2002-07-08 2002-07-08 Reagents and a method for saturation labelling of proteins

Publications (2)

Publication Number Publication Date
CN1650168A CN1650168A (zh) 2005-08-03
CN1307263C true CN1307263C (zh) 2007-03-28

Family

ID=30011660

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB02829498XA Expired - Lifetime CN1307263C (zh) 2002-07-08 2002-07-08 用于饱和标记蛋白质的荧光染料、方法及试剂盒

Country Status (10)

Country Link
US (1) US7511155B2 (enExample)
EP (1) EP1520176B1 (enExample)
JP (1) JP4318180B2 (enExample)
CN (1) CN1307263C (enExample)
AT (1) ATE460670T1 (enExample)
AU (1) AU2002317958B2 (enExample)
CA (1) CA2491692C (enExample)
DE (1) DE60235652D1 (enExample)
ES (1) ES2341946T3 (enExample)
WO (1) WO2004005933A1 (enExample)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102022131451A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Bestimmen einer Signal-Zusammensetzung von Signalfolgen einer Bildfolge
DE102022131444A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren zum Identifizieren von Analyten in einer Bildfolge
DE102022131445A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131447A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131446A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131449A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131448A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131450A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102023122213A1 (de) * 2023-08-18 2025-02-20 Carl Zeiss Microscopy Gmbh Verfahren zum Bereitstellen von Quelldaten zum Trainieren oder Validieren eines Verarbeitungsmodells zum Verarbeiten von Analyt-Bildfolgen

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006062222A1 (ja) * 2004-12-06 2006-06-15 National Institute Of Advanced Industrial Science And Technology タンパク質の分離方法及び染色方法、並びにこれらの方法に用いるタンパク質染色液及びタンパク質染色用キット
US20110186434A1 (en) * 2008-07-23 2011-08-04 Leibniz Institute of Plant Biochemistry Qualitative and/or quantitative determination of a proteinaceous molecule in a plurality of samples
US20110226622A1 (en) * 2008-12-02 2011-09-22 Japan Science And Technology Agency Novel clear native electrophoresis method utilizing aromatic sulfonic acid compound
KR101149535B1 (ko) 2009-04-17 2012-05-25 한국과학기술연구원 생체 분자 표지를 위한 신규 시아닌 화합물 및 그 제조방법
US20130295613A1 (en) * 2010-12-28 2013-11-07 Chugai Seiyaku Kabushiki Kaisha Animal cell culturing method
CN103113282A (zh) * 2013-01-22 2013-05-22 天津理工大学 一种含吸电子基团半菁蓝素温敏分子光学探针的制备方法
CN103113283A (zh) * 2013-01-22 2013-05-22 天津理工大学 一种含给电子基团半菁蓝素温敏分子光学探针的制备方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5268486A (en) * 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US6127134A (en) * 1995-04-20 2000-10-03 Carnegie Mellon University Difference gel electrophoresis using matched multiple dyes
CN1281346A (zh) * 1997-10-10 2001-01-24 西托维亚公司 新型荧光报道分子及其应用,包括对胱天肽酶的测定

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60010956T2 (de) * 1999-03-25 2005-06-16 Nisshinbo Industries, Inc. Carbodiimidverbindungen, die eine fluoreszierende Gruppe enthalten, deren Vorstufen und Verfahren zu deren Herstellung
US6114350A (en) * 1999-04-19 2000-09-05 Nen Life Science Products, Inc. Cyanine dyes and synthesis methods thereof
WO2002026891A1 (en) * 2000-09-29 2002-04-04 Molecular Probes, Inc. Modified carbocyanine dyes and their conjugates
JP2003048879A (ja) * 2001-05-31 2003-02-21 Nisshinbo Ind Inc 蛍光性基含有カルボジイミド化合物及び該化合物の製造方法
US8227621B2 (en) * 2005-06-30 2012-07-24 Li-Cor, Inc. Cyanine dyes and methods of use

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5268486A (en) * 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US6127134A (en) * 1995-04-20 2000-10-03 Carnegie Mellon University Difference gel electrophoresis using matched multiple dyes
CN1281346A (zh) * 1997-10-10 2001-01-24 西托维亚公司 新型荧光报道分子及其应用,包括对胱天肽酶的测定

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CYTOMETRY ERNST L ET AL,3.10,SYANINE DYE LABELING REAGENTS FOR SULFHYDRL FROUPS 1989 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102022131451A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Bestimmen einer Signal-Zusammensetzung von Signalfolgen einer Bildfolge
DE102022131444A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren zum Identifizieren von Analyten in einer Bildfolge
DE102022131445A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131447A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131446A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131449A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131448A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102022131450A1 (de) * 2022-11-28 2024-05-29 Carl Zeiss Microscopy Gmbh Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten
DE102023122213A1 (de) * 2023-08-18 2025-02-20 Carl Zeiss Microscopy Gmbh Verfahren zum Bereitstellen von Quelldaten zum Trainieren oder Validieren eines Verarbeitungsmodells zum Verarbeiten von Analyt-Bildfolgen

Also Published As

Publication number Publication date
CA2491692A1 (en) 2004-01-15
CN1650168A (zh) 2005-08-03
AU2002317958B2 (en) 2007-07-12
ATE460670T1 (de) 2010-03-15
EP1520176A1 (en) 2005-04-06
WO2004005933A1 (en) 2004-01-15
JP4318180B2 (ja) 2009-08-19
US7511155B2 (en) 2009-03-31
EP1520176B1 (en) 2010-03-10
ES2341946T3 (es) 2010-06-30
AU2002317958A1 (en) 2004-01-23
CA2491692C (en) 2010-12-07
DE60235652D1 (de) 2010-04-22
US20050233462A1 (en) 2005-10-20
JP2005532543A (ja) 2005-10-27

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Effective date of registration: 20201014

Address after: Freiburg

Patentee after: GE Healthcare Europe Ltd.

Address before: British platinum County

Patentee before: GE HEALTHCARE UK Ltd.

Effective date of registration: 20201014

Address after: Dassel, Germany

Patentee after: Global life sciences solutions Germany Ltd.

Address before: Freiburg

Patentee before: GE Healthcare Europe Ltd.

CX01 Expiry of patent term
CX01 Expiry of patent term

Granted publication date: 20070328