DE60235652D1 - Reagenzien sowie ein verfahren zur sättigungsmarkierung von proteinen - Google Patents
Reagenzien sowie ein verfahren zur sättigungsmarkierung von proteinenInfo
- Publication number
- DE60235652D1 DE60235652D1 DE60235652T DE60235652T DE60235652D1 DE 60235652 D1 DE60235652 D1 DE 60235652D1 DE 60235652 T DE60235652 T DE 60235652T DE 60235652 T DE60235652 T DE 60235652T DE 60235652 D1 DE60235652 D1 DE 60235652D1
- Authority
- DE
- Germany
- Prior art keywords
- protein
- dye
- labeled
- matched
- group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 7
- 108090000623 proteins and genes Proteins 0.000 title abstract 7
- 238000000034 method Methods 0.000 title abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- 238000009738 saturating Methods 0.000 title 1
- 235000018102 proteins Nutrition 0.000 abstract 6
- 239000000975 dye Substances 0.000 abstract 4
- 239000007850 fluorescent dye Substances 0.000 abstract 3
- 229910052739 hydrogen Inorganic materials 0.000 abstract 2
- 235000001014 amino acid Nutrition 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- JXLHNMVSKXFWAO-UHFFFAOYSA-N azane;7-fluoro-2,1,3-benzoxadiazole-4-sulfonic acid Chemical group N.OS(=O)(=O)C1=CC=C(F)C2=NON=C12 JXLHNMVSKXFWAO-UHFFFAOYSA-N 0.000 abstract 1
- 125000004432 carbon atom Chemical group C* 0.000 abstract 1
- 235000018417 cysteine Nutrition 0.000 abstract 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 abstract 1
- 239000001257 hydrogen Substances 0.000 abstract 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 125000005647 linker group Chemical group 0.000 abstract 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 abstract 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-N sulfonic acid Chemical group OS(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/13—Labelling of peptides
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
- C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0033—Blends of pigments; Mixtured crystals; Solid solutions
- C09B67/0034—Mixtures of two or more pigments or dyes of the same type
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6806—Determination of free amino acids
- G01N33/6812—Assays for specific amino acids
- G01N33/6815—Assays for specific amino acids containing sulfur, e.g. cysteine, cystine, methionine, homocysteine
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Plural Heterocyclic Compounds (AREA)
- Peptides Or Proteins (AREA)
- Indole Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/GB2002/003142 WO2004005933A1 (en) | 2002-07-08 | 2002-07-08 | Reagents and a method for saturation labelling of proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60235652D1 true DE60235652D1 (de) | 2010-04-22 |
Family
ID=30011660
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60235652T Expired - Lifetime DE60235652D1 (de) | 2002-07-08 | 2002-07-08 | Reagenzien sowie ein verfahren zur sättigungsmarkierung von proteinen |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7511155B2 (enExample) |
| EP (1) | EP1520176B1 (enExample) |
| JP (1) | JP4318180B2 (enExample) |
| CN (1) | CN1307263C (enExample) |
| AT (1) | ATE460670T1 (enExample) |
| AU (1) | AU2002317958B2 (enExample) |
| CA (1) | CA2491692C (enExample) |
| DE (1) | DE60235652D1 (enExample) |
| ES (1) | ES2341946T3 (enExample) |
| WO (1) | WO2004005933A1 (enExample) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006062222A1 (ja) * | 2004-12-06 | 2006-06-15 | National Institute Of Advanced Industrial Science And Technology | タンパク質の分離方法及び染色方法、並びにこれらの方法に用いるタンパク質染色液及びタンパク質染色用キット |
| US20110186434A1 (en) * | 2008-07-23 | 2011-08-04 | Leibniz Institute of Plant Biochemistry | Qualitative and/or quantitative determination of a proteinaceous molecule in a plurality of samples |
| US20110226622A1 (en) * | 2008-12-02 | 2011-09-22 | Japan Science And Technology Agency | Novel clear native electrophoresis method utilizing aromatic sulfonic acid compound |
| KR101149535B1 (ko) | 2009-04-17 | 2012-05-25 | 한국과학기술연구원 | 생체 분자 표지를 위한 신규 시아닌 화합물 및 그 제조방법 |
| US20130295613A1 (en) * | 2010-12-28 | 2013-11-07 | Chugai Seiyaku Kabushiki Kaisha | Animal cell culturing method |
| CN103113282A (zh) * | 2013-01-22 | 2013-05-22 | 天津理工大学 | 一种含吸电子基团半菁蓝素温敏分子光学探针的制备方法 |
| CN103113283A (zh) * | 2013-01-22 | 2013-05-22 | 天津理工大学 | 一种含给电子基团半菁蓝素温敏分子光学探针的制备方法 |
| DE102022131445A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131447A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131448A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131449A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131450A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102022131444A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren zum Identifizieren von Analyten in einer Bildfolge |
| DE102022131451A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Bestimmen einer Signal-Zusammensetzung von Signalfolgen einer Bildfolge |
| DE102022131446A1 (de) * | 2022-11-28 | 2024-05-29 | Carl Zeiss Microscopy Gmbh | Verfahren und Vorrichtung zum Aufbereiten von Daten zum Identifizieren von Analyten |
| DE102023122213A1 (de) * | 2023-08-18 | 2025-02-20 | Carl Zeiss Microscopy Gmbh | Verfahren zum Bereitstellen von Quelldaten zum Trainieren oder Validieren eines Verarbeitungsmodells zum Verarbeiten von Analyt-Bildfolgen |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5268486A (en) * | 1986-04-18 | 1993-12-07 | Carnegie-Mellon Unversity | Method for labeling and detecting materials employing arylsulfonate cyanine dyes |
| US6127134A (en) * | 1995-04-20 | 2000-10-03 | Carnegie Mellon University | Difference gel electrophoresis using matched multiple dyes |
| BR9814816A (pt) * | 1997-10-10 | 2004-06-22 | Cytovia Inc | Novas moléculas repórter fluorescentes e suas aplicações incluindo análises de caspases |
| DE60010956T2 (de) * | 1999-03-25 | 2005-06-16 | Nisshinbo Industries, Inc. | Carbodiimidverbindungen, die eine fluoreszierende Gruppe enthalten, deren Vorstufen und Verfahren zu deren Herstellung |
| US6114350A (en) * | 1999-04-19 | 2000-09-05 | Nen Life Science Products, Inc. | Cyanine dyes and synthesis methods thereof |
| WO2002026891A1 (en) * | 2000-09-29 | 2002-04-04 | Molecular Probes, Inc. | Modified carbocyanine dyes and their conjugates |
| JP2003048879A (ja) * | 2001-05-31 | 2003-02-21 | Nisshinbo Ind Inc | 蛍光性基含有カルボジイミド化合物及び該化合物の製造方法 |
| US8227621B2 (en) * | 2005-06-30 | 2012-07-24 | Li-Cor, Inc. | Cyanine dyes and methods of use |
-
2002
- 2002-07-08 CN CNB02829498XA patent/CN1307263C/zh not_active Expired - Lifetime
- 2002-07-08 AT AT02747568T patent/ATE460670T1/de not_active IP Right Cessation
- 2002-07-08 US US10/519,433 patent/US7511155B2/en not_active Expired - Lifetime
- 2002-07-08 JP JP2004518895A patent/JP4318180B2/ja not_active Expired - Lifetime
- 2002-07-08 AU AU2002317958A patent/AU2002317958B2/en not_active Expired
- 2002-07-08 EP EP02747568A patent/EP1520176B1/en not_active Expired - Lifetime
- 2002-07-08 ES ES02747568T patent/ES2341946T3/es not_active Expired - Lifetime
- 2002-07-08 CA CA2491692A patent/CA2491692C/en not_active Expired - Lifetime
- 2002-07-08 WO PCT/GB2002/003142 patent/WO2004005933A1/en not_active Ceased
- 2002-07-08 DE DE60235652T patent/DE60235652D1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CA2491692A1 (en) | 2004-01-15 |
| CN1650168A (zh) | 2005-08-03 |
| AU2002317958B2 (en) | 2007-07-12 |
| ATE460670T1 (de) | 2010-03-15 |
| EP1520176A1 (en) | 2005-04-06 |
| WO2004005933A1 (en) | 2004-01-15 |
| JP4318180B2 (ja) | 2009-08-19 |
| US7511155B2 (en) | 2009-03-31 |
| EP1520176B1 (en) | 2010-03-10 |
| ES2341946T3 (es) | 2010-06-30 |
| AU2002317958A1 (en) | 2004-01-23 |
| CA2491692C (en) | 2010-12-07 |
| US20050233462A1 (en) | 2005-10-20 |
| CN1307263C (zh) | 2007-03-28 |
| JP2005532543A (ja) | 2005-10-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE60235652D1 (de) | Reagenzien sowie ein verfahren zur sättigungsmarkierung von proteinen | |
| DK1141399T3 (da) | Sekventeringsfremgangsmåde under anvendelse af forstörrelsesmærker | |
| DE69942451D1 (de) | Methode zum schnellen identifizieren von kleinen organischen liganden | |
| CO4810232A1 (es) | Citoquinas de mamiferos y sus secuencias codificadoras | |
| DE69701671D1 (de) | Verfahren und zusammenstellungen zur sequenzbestimmung von nukleinsäuremolekulen | |
| CY1111278T1 (el) | Συστημα ασφαλειας | |
| DE60213256D1 (de) | Verfahren zur bestimmung von mehrere analyten | |
| ATE541829T1 (de) | Mit stabilen isotopen markierte aminosäure, verfahren zu deren einschleusung in ein zielprotein, verfahren zur nmr-strukturanalyse von protein | |
| IL162568A0 (en) | fluorescent protein from aequorea coerulscens and uses thereof | |
| CU23302A1 (es) | Medio de cultivo selectivo para el aislamiento y detecciã"n de especies del gã0/00nero streptococcus | |
| ATE483161T1 (de) | Zur vereinfachung komplexer peptidgemische geeignete verfahren und kits | |
| DE69924147D1 (de) | Floureszenzintensitäts-test für eine protein- oder peptidbindung an nukleinsäuren | |
| DE60002989D1 (de) | Verfahren zur herstellung von morpholinonukleotiden und verwendung derselben zur analyse und markierung von nukleinsäuresequenzen | |
| EP1437338A4 (en) | ILLUMINATORS AND MARKING REAGENTS FOR WHICH THEY ARE USED | |
| DE69421849D1 (de) | Verfahren zur Herstellung von alpha,omega-Diaminosäuren abgeleiteten Harnstoffen | |
| DE69819538D1 (de) | Verfahren zur verbesserung der optischen reinheit von 2r-[1-hydroxy-1-trifluormethyl-3-cyclopropylpropyn-2-yl]-4-chloranilin | |
| PT95374A (pt) | Processo para a preparacao de anticorpos contra sequencias de aminoacidos altamente conservadas de substancias imunogenicas bem como de meios de ensaio imunologico que contenham esses anticorpos | |
| RU94002991A (ru) | Способ идентификации мумиеподобных веществ | |
| ECSP982602A (es) | Citoquinas de mamiferos, reactivos relacionados | |
| Wilchek et al. | A. Zamir, I. Ginzburg, N. Sonenberg, R. Miskin | |
| ATE291641T1 (de) | Verfahren zur erstellung von profilen der differentialen proteinexpression | |
| DE60000965D1 (de) | Pseudopeptid, verfahren zur herstellung, reagenz und verwendungen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |