JP2005511004A5 - - Google Patents

Info

Publication number

JP2005511004A5

JP2005511004A5 JP2003508717A JP2003508717A JP2005511004A5 JP 2005511004 A5 JP2005511004 A5 JP 2005511004A5 JP 2003508717 A JP2003508717 A JP 2003508717A JP 2003508717 A JP2003508717 A JP 2003508717A JP 2005511004 A5 JP2005511004 A5 JP 2005511004A5

Authority

JP

Japan

Prior art keywords

seq

oligonucleotide

sequence

nucleic acid

primer

Prior art date

2002-06-28

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Pending

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• 239000002773 nucleotide Substances 0.000 claims description 33
• 125000003729 nucleotide group Chemical group 0.000 claims description 29
• 239000000523 sample Substances 0.000 claims description 15
• 230000000692 anti-sense effect Effects 0.000 claims description 10
• 241000702617 Human parvovirus B19 Species 0.000 claims description 9
• 238000003556 assay Methods 0.000 claims description 4
• 230000003321 amplification Effects 0.000 claims description 3
• 238000003199 nucleic acid amplification method Methods 0.000 claims description 3
• 108091034117 Oligonucleotide Proteins 0.000 claims 48
• 238000000034 method Methods 0.000 claims 26
• 108020004707 nucleic acids Proteins 0.000 claims 18
• 102000039446 nucleic acids Human genes 0.000 claims 18
• 150000007523 nucleic acids Chemical class 0.000 claims 18
• JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 10
• 239000012472 biological sample Substances 0.000 claims 9
• 239000011324 bead Substances 0.000 claims 5
• BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 claims 4
• 239000007787 solid Substances 0.000 claims 4
• WGTODYJZXSJIAG-UHFFFAOYSA-N tetramethylrhodamine chloride Chemical compound [Cl-].C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C(O)=O WGTODYJZXSJIAG-UHFFFAOYSA-N 0.000 claims 4
• 101710163270 Nuclease Proteins 0.000 claims 3
• 108091033319 polynucleotide Proteins 0.000 claims 3
• 102000040430 polynucleotide Human genes 0.000 claims 3
• 239000002157 polynucleotide Substances 0.000 claims 3
• 241000484121 Human parvovirus Species 0.000 claims 2
• 108091028043 Nucleic acid sequence Proteins 0.000 claims 2
• 108091081021 Sense strand Proteins 0.000 claims 2
• 230000000295 complement effect Effects 0.000 claims 2
• 239000007850 fluorescent dye Substances 0.000 claims 2
• 229920001519 homopolymer Polymers 0.000 claims 2
• 230000001404 mediated effect Effects 0.000 claims 1
• 238000003757 reverse transcription PCR Methods 0.000 claims 1
• 238000013518 transcription Methods 0.000 claims 1
• 230000035897 transcription Effects 0.000 claims 1
• 102000004190 Enzymes Human genes 0.000 description 2
• 108090000790 Enzymes Proteins 0.000 description 2
• 239000012634 fragment Substances 0.000 description 2
• 239000013612 plasmid Substances 0.000 description 2
• 238000012408 PCR amplification Methods 0.000 description 1
• MGPYJVWEJNTXLC-UHFFFAOYSA-N [6-[6-[2-cyanoethoxy-[di(propan-2-yl)amino]phosphanyl]oxyhexylcarbamoyl]-6'-(2,2-dimethylpropanoyloxy)-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl] 2,2-dimethylpropanoate Chemical group C12=CC=C(OC(=O)C(C)(C)C)C=C2OC2=CC(OC(=O)C(C)(C)C)=CC=C2C11OC(=O)C2=CC=C(C(=O)NCCCCCCOP(N(C(C)C)C(C)C)OCCC#N)C=C21 MGPYJVWEJNTXLC-UHFFFAOYSA-N 0.000 description 1
• PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
• 239000010931 gold Substances 0.000 description 1
• 229910052737 gold Inorganic materials 0.000 description 1
• 239000011541 reaction mixture Substances 0.000 description 1
• 230000035945 sensitivity Effects 0.000 description 1
• 238000013207 serial dilution Methods 0.000 description 1