JP2005278628A5 - - Google Patents

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JP2005278628A5
JP2005278628A5 JP2004227768A JP2004227768A JP2005278628A5 JP 2005278628 A5 JP2005278628 A5 JP 2005278628A5 JP 2004227768 A JP2004227768 A JP 2004227768A JP 2004227768 A JP2004227768 A JP 2004227768A JP 2005278628 A5 JP2005278628 A5 JP 2005278628A5
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Japan
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antibody
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substance
human
flow cytometry
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JP2004227768A
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JP4498054B2 (ja
JP2005278628A (ja
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Publication of JP2005278628A5 publication Critical patent/JP2005278628A5/ja
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Description

生体においてアレルギー等を誘発する物質(感作性物質)を評価する方法としては、実験動物に被験物質を適用し、そして該実験動物の皮膚などに生ずる反応を視察る方法が行われている。しかしながら、この方法は動物愛護等の見地から見直しがせまられている。
培養(インキュベーション)が終了した後のMIP-1を発現する細胞の表面に発現されたCD86分子の検出又は測定を併用する本発明の好適な態様において、CD86の検出又は測定方法は特に限定されないが、抗−ヒトCD86抗体を用いた免疫測定が好ましく、フローサイトメトリー法が特に好ましい。抗−ヒトCD86抗体はモノクローナル抗体でもポリクローナル抗体でもよく、CD86を表面に発現している細胞を免疫原として用いた常法に従って調製することができる。フローサイトメトリー法も常法に従って行うことができる。
JP2004227768A 2004-03-05 2004-08-04 感作性物質のインビトロ評価法 Expired - Fee Related JP4498054B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2004227768A JP4498054B2 (ja) 2004-03-05 2004-08-04 感作性物質のインビトロ評価法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2004062476 2004-03-05
JP2004227768A JP4498054B2 (ja) 2004-03-05 2004-08-04 感作性物質のインビトロ評価法

Publications (3)

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JP2005278628A JP2005278628A (ja) 2005-10-13
JP2005278628A5 true JP2005278628A5 (ja) 2007-03-29
JP4498054B2 JP4498054B2 (ja) 2010-07-07

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JP2004227768A Expired - Fee Related JP4498054B2 (ja) 2004-03-05 2004-08-04 感作性物質のインビトロ評価法

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JP (1) JP4498054B2 (ja)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4842105B2 (ja) * 2006-11-21 2011-12-21 株式会社 資生堂 光感作性、光毒性及び/又は感作性の評価方法
DE102007006736B4 (de) 2007-02-07 2012-01-12 Dagmar Briechle In-vitro Testkit zur tierversuchsfreien Bestimmung des sensibilisierenden Potentials einer Substanz
KR101349249B1 (ko) 2007-08-24 2014-01-10 (주)아모레퍼시픽 시험관내 피부 감작성 평가 방법

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4270702B2 (ja) * 2000-02-07 2009-06-03 株式会社資生堂 感作性物質のインビトロ評価法

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