JP2003530859A - 固定化酵素基材を使用した細菌およびバクテリオファージの検出 - Google Patents
固定化酵素基材を使用した細菌およびバクテリオファージの検出Info
- Publication number
- JP2003530859A JP2003530859A JP2001577511A JP2001577511A JP2003530859A JP 2003530859 A JP2003530859 A JP 2003530859A JP 2001577511 A JP2001577511 A JP 2001577511A JP 2001577511 A JP2001577511 A JP 2001577511A JP 2003530859 A JP2003530859 A JP 2003530859A
- Authority
- JP
- Japan
- Prior art keywords
- phage
- reaction mixture
- enzyme substrate
- bacteriophage
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 239000000758 substrate Substances 0.000 title claims abstract description 98
- 241000894006 Bacteria Species 0.000 title claims abstract description 74
- 241001515965 unidentified phage Species 0.000 title claims abstract description 71
- 108010093096 Immobilized Enzymes Proteins 0.000 title claims abstract description 47
- 238000001514 detection method Methods 0.000 title description 10
- 238000000034 method Methods 0.000 claims abstract description 53
- 102000004190 Enzymes Human genes 0.000 claims description 89
- 108090000790 Enzymes Proteins 0.000 claims description 89
- 239000011541 reaction mixture Substances 0.000 claims description 56
- 230000001580 bacterial effect Effects 0.000 claims description 34
- 239000007787 solid Substances 0.000 claims description 23
- PBCQXKZFPXBUSL-UHFFFAOYSA-N 2-(2-oxochromen-4-yl)acetic acid Chemical group C1=CC=CC2=C1OC(=O)C=C2CC(=O)O PBCQXKZFPXBUSL-UHFFFAOYSA-N 0.000 claims description 20
- 239000011324 bead Substances 0.000 claims description 20
- 210000002443 helper t lymphocyte Anatomy 0.000 claims description 19
- 238000012258 culturing Methods 0.000 claims description 16
- 238000012544 monitoring process Methods 0.000 claims description 12
- 239000003443 antiviral agent Substances 0.000 claims description 9
- 241000607598 Vibrio Species 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 6
- 230000003993 interaction Effects 0.000 claims description 6
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 4
- 241000588807 Bordetella Species 0.000 claims description 4
- 241000589562 Brucella Species 0.000 claims description 4
- 241000589876 Campylobacter Species 0.000 claims description 4
- 241000606161 Chlamydia Species 0.000 claims description 4
- 241000588881 Chromobacterium Species 0.000 claims description 4
- 241000193403 Clostridium Species 0.000 claims description 4
- 241000186216 Corynebacterium Species 0.000 claims description 4
- 241000588914 Enterobacter Species 0.000 claims description 4
- 241000588722 Escherichia Species 0.000 claims description 4
- 241000606790 Haemophilus Species 0.000 claims description 4
- 241000588748 Klebsiella Species 0.000 claims description 4
- 241000186781 Listeria Species 0.000 claims description 4
- 241000186359 Mycobacterium Species 0.000 claims description 4
- 241000204031 Mycoplasma Species 0.000 claims description 4
- 241000588653 Neisseria Species 0.000 claims description 4
- 241000588769 Proteus <enterobacteria> Species 0.000 claims description 4
- 241000588768 Providencia Species 0.000 claims description 4
- 241000589516 Pseudomonas Species 0.000 claims description 4
- 241000607142 Salmonella Species 0.000 claims description 4
- 241000607768 Shigella Species 0.000 claims description 4
- 241000607734 Yersinia <bacteria> Species 0.000 claims description 4
- 241000607720 Serratia Species 0.000 claims description 3
- 241000193998 Streptococcus pneumoniae Species 0.000 claims description 3
- 230000002934 lysing effect Effects 0.000 claims description 3
- 229940031000 streptococcus pneumoniae Drugs 0.000 claims description 3
- 241000191940 Staphylococcus Species 0.000 claims description 2
- 241000194017 Streptococcus Species 0.000 claims description 2
- 238000004020 luminiscence type Methods 0.000 claims 2
- 238000007747 plating Methods 0.000 claims 2
- 229940088598 enzyme Drugs 0.000 description 77
- 239000000969 carrier Substances 0.000 description 17
- 210000004027 cell Anatomy 0.000 description 17
- 241000588724 Escherichia coli Species 0.000 description 13
- IEJPPSMHUUQABK-UHFFFAOYSA-N 2,4-diphenyl-4h-1,3-oxazol-5-one Chemical group O=C1OC(C=2C=CC=CC=2)=NC1C1=CC=CC=C1 IEJPPSMHUUQABK-UHFFFAOYSA-N 0.000 description 12
- 238000003556 assay Methods 0.000 description 10
- 108010005774 beta-Galactosidase Proteins 0.000 description 8
- 238000005259 measurement Methods 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 239000000203 mixture Substances 0.000 description 7
- 239000000872 buffer Substances 0.000 description 6
- 230000008859 change Effects 0.000 description 6
- 125000000524 functional group Chemical group 0.000 description 6
- 125000006850 spacer group Chemical group 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 230000003321 amplification Effects 0.000 description 5
- 238000003199 nucleic acid amplification method Methods 0.000 description 5
- 239000008188 pellet Substances 0.000 description 5
- 239000011148 porous material Substances 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 102000004316 Oxidoreductases Human genes 0.000 description 4
- 108090000854 Oxidoreductases Proteins 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Substances CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-FPRJBGLDSA-N beta-D-galactose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-FPRJBGLDSA-N 0.000 description 4
- 230000009089 cytolysis Effects 0.000 description 4
- 239000000835 fiber Substances 0.000 description 4
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 230000007505 plaque formation Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 125000003277 amino group Chemical group 0.000 description 3
- 102000005936 beta-Galactosidase Human genes 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000007822 coupling agent Substances 0.000 description 3
- 230000000593 degrading effect Effects 0.000 description 3
- 239000000975 dye Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- NAQMVNRVTILPCV-UHFFFAOYSA-N hexane-1,6-diamine Chemical compound NCCCCCCN NAQMVNRVTILPCV-UHFFFAOYSA-N 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- NPZTUJOABDZTLV-UHFFFAOYSA-N hydroxybenzotriazole Substances O=C1C=CC=C2NNN=C12 NPZTUJOABDZTLV-UHFFFAOYSA-N 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- ASOKPJOREAFHNY-UHFFFAOYSA-N 1-Hydroxybenzotriazole Chemical compound C1=CC=C2N(O)N=NC2=C1 ASOKPJOREAFHNY-UHFFFAOYSA-N 0.000 description 2
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 2
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 description 2
- 102000053187 Glucuronidase Human genes 0.000 description 2
- 108010060309 Glucuronidase Proteins 0.000 description 2
- 229920002907 Guar gum Polymers 0.000 description 2
- 102000004157 Hydrolases Human genes 0.000 description 2
- 108090000604 Hydrolases Proteins 0.000 description 2
- 102000004195 Isomerases Human genes 0.000 description 2
- 108090000769 Isomerases Proteins 0.000 description 2
- 102000004317 Lyases Human genes 0.000 description 2
- 108090000856 Lyases Proteins 0.000 description 2
- 102000016943 Muramidase Human genes 0.000 description 2
- 108010014251 Muramidase Proteins 0.000 description 2
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 description 2
- 102000004357 Transferases Human genes 0.000 description 2
- 108090000992 Transferases Proteins 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000000840 anti-viral effect Effects 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 239000000665 guar gum Substances 0.000 description 2
- 235000010417 guar gum Nutrition 0.000 description 2
- 229960002154 guar gum Drugs 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 229960000274 lysozyme Drugs 0.000 description 2
- 239000004325 lysozyme Substances 0.000 description 2
- 235000010335 lysozyme Nutrition 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 230000001293 nucleolytic effect Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000013307 optical fiber Substances 0.000 description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 230000003595 spectral effect Effects 0.000 description 2
- 238000007655 standard test method Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 125000003396 thiol group Chemical group [H]S* 0.000 description 2
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 1
- WYTZZXDRDKSJID-UHFFFAOYSA-N (3-aminopropyl)triethoxysilane Chemical compound CCO[Si](OCC)(OCC)CCCN WYTZZXDRDKSJID-UHFFFAOYSA-N 0.000 description 1
- PJUPKRYGDFTMTM-UHFFFAOYSA-N 1-hydroxybenzotriazole;hydrate Chemical compound O.C1=CC=C2N(O)N=NC2=C1 PJUPKRYGDFTMTM-UHFFFAOYSA-N 0.000 description 1
- VQKDJSXHVSAIAR-UHFFFAOYSA-N 1h-imidazol-2-yl carbamate Chemical class NC(=O)OC1=NC=CN1 VQKDJSXHVSAIAR-UHFFFAOYSA-N 0.000 description 1
- PQZLOWONDMXBIN-UHFFFAOYSA-N 2-(1-adamantyl)oxirane Chemical class C1OC1C1(C2)CC(C3)CC2CC3C1 PQZLOWONDMXBIN-UHFFFAOYSA-N 0.000 description 1
- BNHPMQBVNXMPDU-UHFFFAOYSA-N 2-(7-hydroxy-2-oxochromen-4-yl)acetic acid Chemical compound C1=C(O)C=CC2=C1OC(=O)C=C2CC(=O)O BNHPMQBVNXMPDU-UHFFFAOYSA-N 0.000 description 1
- GOLORTLGFDVFDW-UHFFFAOYSA-N 3-(1h-benzimidazol-2-yl)-7-(diethylamino)chromen-2-one Chemical compound C1=CC=C2NC(C3=CC4=CC=C(C=C4OC3=O)N(CC)CC)=NC2=C1 GOLORTLGFDVFDW-UHFFFAOYSA-N 0.000 description 1
- FPQQSJJWHUJYPU-UHFFFAOYSA-N 3-(dimethylamino)propyliminomethylidene-ethylazanium;chloride Chemical compound Cl.CCN=C=NCCCN(C)C FPQQSJJWHUJYPU-UHFFFAOYSA-N 0.000 description 1
- OBJOZRVSMLPASY-UHFFFAOYSA-N 8-hydroxypyrene-1,3,6-trisulfonic acid Chemical compound C1=C2C(O)=CC(S(O)(=O)=O)=C(C=C3)C2=C2C3=C(S(O)(=O)=O)C=C(S(O)(=O)=O)C2=C1 OBJOZRVSMLPASY-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 241000935254 Enterobacteria phage RB33 Species 0.000 description 1
- 108010093031 Galactosidases Proteins 0.000 description 1
- 102000002464 Galactosidases Human genes 0.000 description 1
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical class [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 229920000161 Locust bean gum Polymers 0.000 description 1
- 239000006137 Luria-Bertani broth Substances 0.000 description 1
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical class ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 1
- 108010058846 Ovalbumin Proteins 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 125000005282 allenyl group Chemical group 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O ammonium group Chemical group [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229910010293 ceramic material Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 125000000753 cycloalkyl group Chemical group 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000014670 detection of bacterium Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 150000004985 diamines Chemical class 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 125000001033 ether group Chemical group 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 238000002189 fluorescence spectrum Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 125000005842 heteroatom Chemical group 0.000 description 1
- 239000012510 hollow fiber Substances 0.000 description 1
- 229920001477 hydrophilic polymer Polymers 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- PCKPVGOLPKLUHR-UHFFFAOYSA-N indoxyl Chemical group C1=CC=C2C(O)=CNC2=C1 PCKPVGOLPKLUHR-UHFFFAOYSA-N 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- -1 iodoacetyl groups Chemical group 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000000711 locust bean gum Substances 0.000 description 1
- 235000010420 locust bean gum Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- UPSFMJHZUCSEHU-JYGUBCOQSA-N n-[(2s,3r,4r,5s,6r)-2-[(2r,3s,4r,5r,6s)-5-acetamido-4-hydroxy-2-(hydroxymethyl)-6-(4-methyl-2-oxochromen-7-yl)oxyoxan-3-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)oxan-3-yl]acetamide Chemical compound CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H]1[C@H](O)[C@@H](NC(C)=O)[C@H](OC=2C=C3OC(=O)C=C(C)C3=CC=2)O[C@@H]1CO UPSFMJHZUCSEHU-JYGUBCOQSA-N 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000012038 nucleophile Substances 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 239000001575 punica granatum l. bark extract Substances 0.000 description 1
- 238000012207 quantitative assay Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 125000006413 ring segment Chemical group 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000007086 side reaction Methods 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 125000001273 sulfonato group Chemical group [O-]S(*)(=O)=O 0.000 description 1
- 125000000472 sulfonyl group Chemical group *S(*)(=O)=O 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000000230 xanthan gum Substances 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
- 235000010493 xanthan gum Nutrition 0.000 description 1
- 229940082509 xanthan gum Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
- C12Q2334/20—Coumarin derivatives
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/548,157 US6436661B1 (en) | 2000-04-13 | 2000-04-13 | Bacteria and bacteriophage detection using immobilized enzyme substrates |
| US09/548,157 | 2000-04-13 | ||
| PCT/US2000/022277 WO2001079528A1 (en) | 2000-04-13 | 2000-08-14 | Bacteria and bacteriophage detection using immobilized enzyme substrates |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2003530859A true JP2003530859A (ja) | 2003-10-21 |
| JP2003530859A5 JP2003530859A5 (enExample) | 2007-08-30 |
Family
ID=24187649
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001577511A Withdrawn JP2003530859A (ja) | 2000-04-13 | 2000-08-14 | 固定化酵素基材を使用した細菌およびバクテリオファージの検出 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US6436661B1 (enExample) |
| EP (1) | EP1272658B1 (enExample) |
| JP (1) | JP2003530859A (enExample) |
| AT (1) | ATE263253T1 (enExample) |
| AU (1) | AU2000269056A1 (enExample) |
| CA (1) | CA2405732A1 (enExample) |
| DE (1) | DE60009575T2 (enExample) |
| WO (1) | WO2001079528A1 (enExample) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012044957A (ja) * | 2010-08-30 | 2012-03-08 | Mitsubishi Electric Corp | 浮遊ウイルス不活化評価方法およびその装置 |
| JP2015535683A (ja) * | 2012-09-19 | 2015-12-17 | ユニバーサル バイオセンサーズ ピーティーワイ リミテッドUniversal Biosensors Pty Limited | 酵素検出のためのシステム及び方法 |
| KR102065620B1 (ko) * | 2019-01-21 | 2020-01-13 | 주식회사 마이크로진 | 박테리오파지를 이용한 식중독 세균 검출 키트 및 이를 이용한 검출방법 |
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6436661B1 (en) * | 2000-04-13 | 2002-08-20 | 3M Innovative Properties Company | Bacteria and bacteriophage detection using immobilized enzyme substrates |
| ES2263598T3 (es) * | 2001-02-01 | 2006-12-16 | Profos Ag | Deteccion e identificacion de grupos de bacterias. |
| US20020192676A1 (en) * | 2001-06-18 | 2002-12-19 | Madonna Angelo J. | Method for determining if a type of bacteria is present in a mixture |
| CA2353307A1 (fr) * | 2001-07-13 | 2003-01-13 | Carmen Parent | Appareil et procede pour le traitement des effluents gazeux |
| CA2474159A1 (en) * | 2002-01-23 | 2003-05-21 | Carl R. Merril | Method for determining sensitivity to a bacteriophage |
| US8216780B2 (en) * | 2002-04-12 | 2012-07-10 | Microphage (Tm) Incorporated | Method for enhanced sensitivity in bacteriophage-based diagnostic assays |
| US20050003346A1 (en) * | 2002-04-12 | 2005-01-06 | Colorado School Of Mines | Apparatus and method for detecting microscopic living organisms using bacteriophage |
| US7166425B2 (en) * | 2002-04-12 | 2007-01-23 | Colorado School Of Mines | Method for detecting low concentrations of a target bacterium that uses phages to infect target bacterial cells |
| EP1556502A4 (en) * | 2002-10-15 | 2006-07-19 | Univ Minnesota | ASSAYS FOR THE DETECTION AND QUANTIFICATION OF BACTERIAL OR VIRAL DISEASES AND POLLUTION |
| EP1613965A2 (en) | 2003-04-10 | 2006-01-11 | Kent J. Voorhees | Apparatus and method for detecting microscopic living organisms using bacteriophage |
| US20090286225A1 (en) * | 2004-02-13 | 2009-11-19 | Microphagetm Incorporated | Method and apparatus for bacteriophage-based diagnostic assays |
| US20050250096A1 (en) * | 2004-02-13 | 2005-11-10 | Microphage, Incorporated | Microorganism detection using bacteriophage amplification |
| CA2586299C (en) | 2004-11-01 | 2014-10-28 | James W. Stave | Method for detecting or enriching a target microorganism upon lysis of non-target bacteria with bacteriophage |
| US20110097702A1 (en) * | 2005-03-31 | 2011-04-28 | Voorhees Kent J | Methods and compositions for in situ detection of microorganisms on a surface |
| WO2006105414A2 (en) * | 2005-03-31 | 2006-10-05 | Colorado School Of Mines | Apparatus and method for detecting microscopic organisms using microphage |
| US20070178450A1 (en) * | 2006-01-27 | 2007-08-02 | Microphage (Tm) Incorporation | Method and apparatus for determining level of microorganisms using bacteriophage |
| WO2008063838A2 (en) * | 2006-10-31 | 2008-05-29 | Microphage Incorporated | Method and apparatus for enhanced bacteriophage-based diagnostic assays by selective inhibition of potential cross-reactive organisms |
| US9376704B2 (en) | 2007-02-12 | 2016-06-28 | Charm Sciences, Inc. | Test kit and method for detecting bacteriophage |
| WO2008143722A2 (en) | 2007-02-12 | 2008-11-27 | Charm Sciences, Inc. | Test device, method and kit for phage detection |
| CA2690809A1 (en) * | 2007-06-15 | 2008-12-24 | Microphage Incorporated | Method of detection of microorganisms with enhanced bacteriophage amplification |
| US8697434B2 (en) * | 2008-01-11 | 2014-04-15 | Colorado School Of Mines | Detection of phage amplification by SERS nanoparticles |
| US9441204B2 (en) * | 2008-04-03 | 2016-09-13 | Colorado School Of Mines | Compositions and methods for detecting Yersinia pestis bacteria |
| WO2011042901A2 (en) | 2009-10-07 | 2011-04-14 | The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization, (A.R.O.), Volcani Center | Brucella phage polynucleotides and uses thereof |
| GB2477752A (en) * | 2010-02-11 | 2011-08-17 | Arab Biotechnology Company | Detection of bacteria |
| WO2012158041A1 (en) | 2011-05-18 | 2012-11-22 | Rna Holding B.V. | Diagnostic methods and kits for determining the presence of a microorganism in a sample |
| US9433239B2 (en) | 2013-03-15 | 2016-09-06 | Altria Client Services Llc | Bacteriophage and methods of making and using |
| EP3068894B1 (en) * | 2013-11-13 | 2019-04-17 | Universitat de Barcelona | Method for detecting bacteriolytic conditions in a sample |
| WO2016123427A1 (en) | 2015-01-29 | 2016-08-04 | Altria Client Services Llc | Bacteriophage and methods of using |
| US10357056B2 (en) | 2015-01-29 | 2019-07-23 | Altria Client Services Llc | Endolysin from bacteriophage against Geobacillus and methods of using |
| DE102015202353B3 (de) * | 2015-02-04 | 2016-03-10 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Vorrichtung und verfahren zur erfassung der resistenz von bakterien gegenüber einem zu analysierenden wirkstoff unter verwendung eines mikrofluidikchips |
| US20190316169A1 (en) * | 2016-11-28 | 2019-10-17 | Sonanutech Inc. | Methods, compositions, and kits for detecting a cell in a sample |
| AU2018308944B2 (en) | 2017-07-23 | 2022-09-01 | Future Biosolutions Pty Ltd | Method for rapid detection and enumeration of viruses, bacteriophage and/or bacteria |
| WO2019099467A1 (en) * | 2017-11-14 | 2019-05-23 | Chapman University School Of Pharmacy (Cusp), Harry And Diane Rinker Health Science Campus | Rapid selective detection of bacteria |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4029597A (en) | 1969-03-14 | 1977-06-14 | E. Merck A. G. | Non-bleeding indicators and dyes therefor used in pH determination process |
| US4104126A (en) | 1976-01-29 | 1978-08-01 | Nichols Institute Of Endocrinology, Inc. | Non-isotopic substrate assay employing bacteriolysis products |
| US4565783A (en) | 1981-01-27 | 1986-01-21 | Minnesota Mining And Manufacturing Company | Dry culture media |
| US4861709A (en) | 1985-05-31 | 1989-08-29 | Technicon Research A.G. | Detection and/or identification of microorganisms in a test sample using bioluminescence or other exogenous genetically-introduced marker |
| US5238809A (en) | 1986-02-03 | 1993-08-24 | Avl Medical Instruments Ag | Method for optical determination of the catalytic enzyme activity and arrangement for implementing this method |
| US5292840A (en) | 1987-03-13 | 1994-03-08 | Minnesota Mining And Manufacturing Company | Polymeric supports |
| GB8724764D0 (en) | 1987-10-22 | 1987-11-25 | Alcan Int Ltd | Enzyme assay method |
| US5089413A (en) | 1989-05-19 | 1992-02-18 | Minnesota Mining And Manufacturing Company | Method and apparatus for culturing with microbiological dry culture medium |
| GB9017443D0 (en) * | 1990-08-09 | 1990-09-26 | Amersham Int Plc | Reporter bacteria for rapid microbial detection |
| GB2255561B (en) * | 1991-04-20 | 1995-06-21 | Agricultural & Food Res | Lysins from bacteriophages |
| US5384411A (en) | 1991-06-20 | 1995-01-24 | Hewlett-Packard Company | Immobilization of PH-sensitive dyes to solid supports |
| WO1993007483A1 (de) | 1991-09-30 | 1993-04-15 | Merck Patent Gmbh | SENSORMEMBRAN ZUR ANZEIGE DES pH-WERTES EINER PROBE, IHRE HERSTELLUNG UND VERWENDUNG |
| US5232838A (en) | 1991-12-09 | 1993-08-03 | Minnesota Mining And Manufacturing Company | Culture media device and method of use |
| US5888725A (en) * | 1992-09-22 | 1999-03-30 | The Secretary Of State For The Minister Of Agriculture Fisheries And Food In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern Ireland | Method for identifying target bacteria |
| US6180135B1 (en) * | 1994-08-11 | 2001-01-30 | The Regents Of The University Of California | Three-dimensional colorimetric assay assemblies |
| US5723308A (en) | 1993-05-14 | 1998-03-03 | Minnesota Mining And Manufacturing Company | Culture medium for rapid count of coliform bacteria |
| GB9317139D0 (en) | 1993-08-18 | 1993-10-06 | Miniser For Agriculture Fisher | Method and test kits for detection of bacteriophage |
| US5561097A (en) | 1994-04-28 | 1996-10-01 | Minnesota Mining And Manufacturing Company | Method of controlling density of ligand coupled onto supports and products produced therefrom |
| US5601998A (en) | 1994-08-18 | 1997-02-11 | Minnesota Mining & Mfg | Culture medium and device for detection and enumeration of enterobacteriaceae |
| GB9525661D0 (en) * | 1995-12-15 | 1996-02-14 | Biotec Diagnostics Limited | Method |
| DE19617338A1 (de) | 1996-04-30 | 1997-11-06 | Sendrowski Peter | Verfahren und Vorrichtung zur Identifikation von Mikroorganismen, Pilzen und Kleinstlebewesen mit Indikatorkugeln |
| US5958675A (en) * | 1997-04-18 | 1999-09-28 | 3M Innovative Properties Company | Method for detecting bacteria using bacteriophage, contrast-coloring dye and precipitable dye |
| US5998593A (en) | 1998-03-10 | 1999-12-07 | Abbott Laboratories | Fluorescent enzyme substrates |
| US6251624B1 (en) * | 1999-03-12 | 2001-06-26 | Akzo Nobel N.V. | Apparatus and method for detecting, quantifying and characterizing microorganisms |
| US6436661B1 (en) * | 2000-04-13 | 2002-08-20 | 3M Innovative Properties Company | Bacteria and bacteriophage detection using immobilized enzyme substrates |
-
2000
- 2000-04-13 US US09/548,157 patent/US6436661B1/en not_active Expired - Lifetime
- 2000-08-14 AT AT00957436T patent/ATE263253T1/de not_active IP Right Cessation
- 2000-08-14 CA CA002405732A patent/CA2405732A1/en not_active Abandoned
- 2000-08-14 AU AU2000269056A patent/AU2000269056A1/en not_active Abandoned
- 2000-08-14 WO PCT/US2000/022277 patent/WO2001079528A1/en not_active Ceased
- 2000-08-14 DE DE60009575T patent/DE60009575T2/de not_active Expired - Fee Related
- 2000-08-14 JP JP2001577511A patent/JP2003530859A/ja not_active Withdrawn
- 2000-08-14 EP EP00957436A patent/EP1272658B1/en not_active Expired - Lifetime
-
2002
- 2002-05-30 US US10/160,594 patent/US6833251B2/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012044957A (ja) * | 2010-08-30 | 2012-03-08 | Mitsubishi Electric Corp | 浮遊ウイルス不活化評価方法およびその装置 |
| JP2015535683A (ja) * | 2012-09-19 | 2015-12-17 | ユニバーサル バイオセンサーズ ピーティーワイ リミテッドUniversal Biosensors Pty Limited | 酵素検出のためのシステム及び方法 |
| KR102065620B1 (ko) * | 2019-01-21 | 2020-01-13 | 주식회사 마이크로진 | 박테리오파지를 이용한 식중독 세균 검출 키트 및 이를 이용한 검출방법 |
Also Published As
| Publication number | Publication date |
|---|---|
| US6833251B2 (en) | 2004-12-21 |
| AU2000269056A1 (en) | 2001-10-30 |
| WO2001079528A1 (en) | 2001-10-25 |
| DE60009575D1 (de) | 2004-05-06 |
| EP1272658A1 (en) | 2003-01-08 |
| ATE263253T1 (de) | 2004-04-15 |
| CA2405732A1 (en) | 2001-10-25 |
| US20030017449A1 (en) | 2003-01-23 |
| DE60009575T2 (de) | 2004-12-30 |
| US6436661B1 (en) | 2002-08-20 |
| EP1272658B1 (en) | 2004-03-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2003530859A (ja) | 固定化酵素基材を使用した細菌およびバクテリオファージの検出 | |
| CA2380480C (en) | Detection and identification of bacterial strains | |
| JP4986178B2 (ja) | 膜上で生細胞を検出するための、nog、hmp、塩化ルビジウム及び/又は塩化リチウムを含む細胞透過処理のための組成物 | |
| EP1687439B1 (en) | Colorimetric substrates, colorimetric sensors, and methods of use | |
| JP5826031B2 (ja) | 黄色ブドウ球菌バクテリア用の反応培地 | |
| JP2011206065A (ja) | 微生物の検出方法 | |
| JP2002500892A (ja) | 抗生物質感受性試験 | |
| US5750357A (en) | Method of rapid analyte detection | |
| JP3733143B2 (ja) | ブドウ球菌を選択的に検出するための増殖培地 | |
| US11085065B2 (en) | Phenotypic engineering of spores | |
| CN101451159A (zh) | 含乙二胺四乙酸和聚乙烯亚胺的组合、能透化微生物细胞壁的新组合物 | |
| WO2007139601A2 (en) | Phenotypic engineering of spore | |
| US8377678B2 (en) | Methodology for detection, enumeration, propagation and manipulation of bacteriophages | |
| CN115725462B (zh) | 一株耐多粘菌素产超广谱β-内酰胺酶大肠杆菌EC382-2-2及其应用 | |
| WO2012154734A1 (en) | System for detecting and enumerating biological particles | |
| JP2004507252A (ja) | サンプル中の標的微生物検出のための組成物および方法 | |
| JP3964178B2 (ja) | メタロ−β−ラクタマーゼ産生菌の薬剤感受性試験方法 | |
| JP4510222B2 (ja) | 細菌の鑑別方法 | |
| JP4602318B2 (ja) | 微生物細胞壁の透過性を増大させるための組成物および膜上で前記微生物を検出するための方法 | |
| JP2023523627A (ja) | メチシリン耐性黄色ブドウ球菌を検出するための組成物、方法およびシステム | |
| CA1108516A (en) | Antibiotic detection method | |
| JP4495809B2 (ja) | 細菌の鑑別方法 | |
| EP1790734A1 (en) | Microbial assay | |
| JPH1132761A (ja) | 変異原性試験方法 | |
| JP2005511091A (ja) | 微生物を検出する方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20070703 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20070703 |
|
| A761 | Written withdrawal of application |
Free format text: JAPANESE INTERMEDIATE CODE: A761 Effective date: 20081022 |