JP2003526361A - Ｈｃｖｎｓ２／３フラグメント及びその使用 - Google Patents

Ｈｃｖｎｓ２／３フラグメント及びその使用

Info

Publication number
JP2003526361A
JP2003526361A JP2001567302A JP2001567302A JP2003526361A JP 2003526361 A JP2003526361 A JP 2003526361A JP 2001567302 A JP2001567302 A JP 2001567302A JP 2001567302 A JP2001567302 A JP 2001567302A JP 2003526361 A JP2003526361 A JP 2003526361A
Authority
JP
Japan
Prior art keywords
hcv
protease
polypeptide
activity
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
JP2001567302A
Other languages
English (en)
Japanese (ja)
Other versions
JP2003526361A5 (enExample
Inventor
シユタインクーラー，クリスチヤン
パツラオーロ，ミケーレ
ラーム，アルミン
Original Assignee
イステイチユート・デイ・リチエルケ・デイ・ビオロジア・モレコラーレ・ピ・アンジエレツテイ・エツセ・ピー・アー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by イステイチユート・デイ・リチエルケ・デイ・ビオロジア・モレコラーレ・ピ・アンジエレツテイ・エツセ・ピー・アー filed Critical イステイチユート・デイ・リチエルケ・デイ・ビオロジア・モレコラーレ・ピ・アンジエレツテイ・エツセ・ピー・アー
Publication of JP2003526361A publication Critical patent/JP2003526361A/ja
Publication of JP2003526361A5 publication Critical patent/JP2003526361A5/ja
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Hydrogenated Pyridines (AREA)
  • Detergent Compositions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2001567302A 2000-03-17 2001-03-14 Ｈｃｖｎｓ２／３フラグメント及びその使用 Withdrawn JP2003526361A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0006537.5 2000-03-17
GBGB0006537.5A GB0006537D0 (en) 2000-03-17 2000-03-17 Assays and screening methods
PCT/IB2001/000527 WO2001068818A2 (en) 2000-03-17 2001-03-14 Hcv ns2/3 fragments and uses thereof

Publications (2)

Publication Number Publication Date
JP2003526361A true JP2003526361A (ja) 2003-09-09
JP2003526361A5 JP2003526361A5 (enExample) 2008-03-13

Family

ID=9887885

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2001567302A Withdrawn JP2003526361A (ja) 2000-03-17 2001-03-14 Ｈｃｖｎｓ２／３フラグメント及びその使用

Country Status (10)

Country Link
US (1) US7078215B2 (enExample)
EP (1) EP1278867B1 (enExample)
JP (1) JP2003526361A (enExample)
AT (1) ATE315093T1 (enExample)
AU (1) AU4269401A (enExample)
CA (1) CA2403405A1 (enExample)
DE (1) DE60116474T2 (enExample)
ES (1) ES2254380T3 (enExample)
GB (1) GB0006537D0 (enExample)
WO (1) WO2001068818A2 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007537150A (ja) * 2004-01-19 2007-12-20 アレス トレーディング ソシエテ アノニム 細菌で発現したタンパク質の精製方法
JP2009513113A (ja) * 2005-10-28 2009-04-02 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング Ｃ型肝炎ウイルスｎｓ２／３活性のアッセイ法
JP2013526284A (ja) * 2010-05-17 2013-06-24 クレティス・アクチェンゲゼルシャフト 身体試料を溶解するための汎用溶解バッファーおよび加工方法

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3878132B2 (ja) 2000-12-15 2007-02-07 ベーリンガー インゲルハイム （カナダ） リミテッド 精製活性型ｈｃｖｎｓ２／３プロテアーゼ
US20090233266A1 (en) * 2005-03-10 2009-09-17 The Rockefeller University Structure of the hepatitis c virus ns2 protein

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5888762A (en) 1990-06-05 1999-03-30 Centre National De La Recherche Scientifique (Cnrs) Neurotropic growth factors comprising a homeobox peptide
IT1277914B1 (it) 1995-08-22 1997-11-12 Angeletti P Ist Richerche Bio Procedimento per produrre - in forma pura e in quantita' elevate - polipeptidi con l'attivita' proteolitica della proteasi ns3 di hcv, e
US5837464A (en) * 1996-01-29 1998-11-17 Virologic, Inc. Compositions and methods for determining anti-viral drug susceptibility and resistance and anti-viral drug screening
US6242187B1 (en) * 1996-01-29 2001-06-05 Virologic, Inc. Compositions and methods for determining anti-viral drug susceptibility and resistance and anti-viral drug screening
US6280940B1 (en) * 1998-08-05 2001-08-28 Agouron Pharmaceuticals, Inc. Reporter gene system for use in cell-based assessment of inhibitors of the Hepatitis C virus protease
CA2383411A1 (en) * 1999-08-30 2001-03-08 Paul L. Darke Hepatitis c virus replication inhibitors
JP3878132B2 (ja) * 2000-12-15 2007-02-07 ベーリンガー インゲルハイム （カナダ） リミテッド 精製活性型ｈｃｖｎｓ２／３プロテアーゼ

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007537150A (ja) * 2004-01-19 2007-12-20 アレス トレーディング ソシエテ アノニム 細菌で発現したタンパク質の精製方法
JP4865569B2 (ja) * 2004-01-19 2012-02-01 アレス トレーディング ソシエテ アノニム 細菌で発現したタンパク質の精製方法
JP2009513113A (ja) * 2005-10-28 2009-04-02 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング Ｃ型肝炎ウイルスｎｓ２／３活性のアッセイ法
JP2013526284A (ja) * 2010-05-17 2013-06-24 クレティス・アクチェンゲゼルシャフト 身体試料を溶解するための汎用溶解バッファーおよび加工方法
US9598721B2 (en) 2010-05-17 2017-03-21 Curetis Gmbh Universally applicable lysis buffer and processing methods for the lysis of bodily samples

Also Published As

Publication number Publication date
ATE315093T1 (de) 2006-02-15
US7078215B2 (en) 2006-07-18
WO2001068818A2 (en) 2001-09-20
DE60116474D1 (de) 2006-03-30
GB0006537D0 (en) 2000-05-10
DE60116474T2 (de) 2006-09-07
CA2403405A1 (en) 2001-09-20
US20040054134A1 (en) 2004-03-18
AU4269401A (en) 2001-09-24
ES2254380T3 (es) 2006-06-16
EP1278867B1 (en) 2006-01-04
WO2001068818A3 (en) 2002-03-21
EP1278867A2 (en) 2003-01-29

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