JP2003525627A5 - - Google Patents
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- Publication number
- JP2003525627A5 JP2003525627A5 JP2001565862A JP2001565862A JP2003525627A5 JP 2003525627 A5 JP2003525627 A5 JP 2003525627A5 JP 2001565862 A JP2001565862 A JP 2001565862A JP 2001565862 A JP2001565862 A JP 2001565862A JP 2003525627 A5 JP2003525627 A5 JP 2003525627A5
- Authority
- JP
- Japan
- Prior art keywords
- rna polymerase
- mutation
- mutated
- amino acid
- amplification reaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000035772 mutation Effects 0.000 description 12
- 101710137500 T7 RNA polymerase Proteins 0.000 description 8
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 4
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 102100034343 Integrase Human genes 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 125000001500 prolyl group Chemical group [H]N1C([H])(C(=O)[*])C([H])([H])C([H])([H])C1([H])[H] 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP00200787.0 | 2000-03-07 | ||
| EP00200787 | 2000-03-07 | ||
| PCT/EP2001/002327 WO2001066705A1 (en) | 2000-03-07 | 2001-03-01 | Rna polymerase mutants with increased thermostability |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2003525627A JP2003525627A (ja) | 2003-09-02 |
| JP2003525627A5 true JP2003525627A5 (https=) | 2008-04-17 |
| JP4832694B2 JP4832694B2 (ja) | 2011-12-07 |
Family
ID=8171152
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001565862A Expired - Lifetime JP4832694B2 (ja) | 2000-03-07 | 2001-03-01 | 増加した熱安定性を有するｒｎａポリメラーゼ変異体 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US7507567B2 (https=) |
| EP (1) | EP1261696B1 (https=) |
| JP (1) | JP4832694B2 (https=) |
| AT (1) | ATE294855T1 (https=) |
| AU (1) | AU2001237425A1 (https=) |
| CA (1) | CA2400049C (https=) |
| DE (1) | DE60110564T2 (https=) |
| ES (1) | ES2242733T3 (https=) |
| WO (1) | WO2001066705A1 (https=) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009136153A (ja) * | 2007-12-03 | 2009-06-25 | Tosoh Corp | 変異型ｒｎａポリメラーゼ |
| WO2009113718A1 (ja) * | 2008-03-11 | 2009-09-17 | 東ソー株式会社 | 機能改善したｒｎａポリメラーゼ変異体 |
| JP5625908B2 (ja) * | 2008-08-08 | 2014-11-19 | 東ソー株式会社 | 機能改善したｒｎａポリメラーゼ変異体 |
| US9193959B2 (en) | 2010-04-16 | 2015-11-24 | Roche Diagnostics Operations, Inc. | T7 RNA polymerase variants with enhanced thermostability |
| EP2611922A1 (en) | 2010-08-31 | 2013-07-10 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways through protease manipulation |
| US9062292B2 (en) | 2010-09-13 | 2015-06-23 | Enzo Life Sciences Inc. | Mutant T7 polymerases |
| FR2981088B1 (fr) | 2011-10-06 | 2013-11-29 | Biomerieux Sa | Arn polymerases mutees |
| US9045740B2 (en) * | 2012-02-24 | 2015-06-02 | University Of Massachusetts | Modified T7-related RNA polymerases and methods of use thereof |
| AU2014306074B2 (en) | 2013-08-05 | 2018-08-30 | Greenlight Biosciences, Inc. | Engineered proteins with a protease cleavage site |
| WO2015024017A2 (en) * | 2013-08-16 | 2015-02-19 | President And Fellows Of Harvard College | Rna polymerase, methods of purification and methods of use |
| KR20160132113A (ko) | 2014-03-20 | 2016-11-16 | 보드 오브 리전츠, 더 유니버시티 오브 텍사스 시스템 | 확장된 기질 범위 및 증진된 전사 수율을 갖는 t7 rna 폴리머라제 변이체 |
| BR112017020690A2 (pt) | 2015-03-30 | 2018-06-26 | Greenlight Biosciences Inc | produção livre de células de ácido ribonucleico |
| EP3317424B1 (en) * | 2015-07-01 | 2023-09-06 | CureVac Manufacturing GmbH | Method for analysis of an rna molecule |
| CN116144626A (zh) * | 2016-01-13 | 2023-05-23 | 新英格兰生物实验室公司 | T7 rna聚合酶的热稳定变体 |
| CA3020312A1 (en) | 2016-04-06 | 2017-10-12 | Greenlight Biosciences, Inc. | Cell-free production of ribonucleic acid |
| US10034951B1 (en) | 2017-06-21 | 2018-07-31 | New England Biolabs, Inc. | Use of thermostable RNA polymerases to produce RNAs having reduced immunogenicity |
| KR20250028535A (ko) | 2017-06-30 | 2025-02-28 | 코덱시스, 인코포레이티드 | T7 rna 폴리머라제 변이체 |
| EP3645712A4 (en) | 2017-06-30 | 2021-07-07 | Codexis, Inc. | T7 RNA POLYMERASE VARIANTS |
| KR102894284B1 (ko) | 2017-10-11 | 2025-12-03 | 그린라이트 바이오사이언시스, 아이엔씨. | 뉴클레오시드 트리포스페이트 및 리보핵산 생산을 위한 방법 및 조성물 |
| CN112218947A (zh) * | 2018-04-10 | 2021-01-12 | 绿光生物科技股份有限公司 | T7 rna聚合酶变体 |
| WO2020243026A1 (en) * | 2019-05-24 | 2020-12-03 | Primordial Genetics Inc. | Methods and compositions for manufacturing polynucleotides |
| CN112980811B (zh) * | 2021-03-06 | 2022-02-25 | 苏州瀚源新酶生物科技有限公司 | Rna聚合酶突变体及其应用、重组载体及其制备方法和应用、重组工程菌及其应用 |
| EP4108770A1 (en) | 2021-06-24 | 2022-12-28 | Aptamimetics GmbH | Age-selex method for aptamer identification and use |
| WO2025127053A1 (ja) * | 2023-12-12 | 2025-06-19 | タカラバイオ株式会社 | Ｒｎａポリメラーゼ変異体とｒｎａ合成 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4952496A (en) * | 1984-03-30 | 1990-08-28 | Associated Universities, Inc. | Cloning and expression of the gene for bacteriophage T7 RNA polymerase |
| CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| US5385834A (en) * | 1993-08-13 | 1995-01-31 | Georgia Tech Research Corporation | Mutant T7 RNA polymerase GP1(lys222) exhibiting altered promoter recognition |
| FR2761695B1 (fr) * | 1997-04-04 | 1999-09-24 | Bio Merieux | Arn polymerase fonctionnant preferentiellement sur matrice d'arn, sa preparation et son utilisation |
| JP3172710B2 (ja) * | 1997-07-07 | 2001-06-04 | 理化学研究所 | Ｒｎａポリメラーゼ |
| ATE266729T1 (de) * | 1998-12-11 | 2004-05-15 | Biomerieux Bv | Rna polymerase mutanten mit erhöhter stabilität |
| JP4399684B2 (ja) * | 1999-08-17 | 2010-01-20 | 東洋紡績株式会社 | 改良されたｒｎａポリメラーゼ |
-
2001
- 2001-03-01 CA CA2400049A patent/CA2400049C/en not_active Expired - Lifetime
- 2001-03-01 DE DE60110564T patent/DE60110564T2/de not_active Expired - Lifetime
- 2001-03-01 ES ES01909810T patent/ES2242733T3/es not_active Expired - Lifetime
- 2001-03-01 AU AU2001237425A patent/AU2001237425A1/en not_active Abandoned
- 2001-03-01 EP EP01909810A patent/EP1261696B1/en not_active Expired - Lifetime
- 2001-03-01 WO PCT/EP2001/002327 patent/WO2001066705A1/en not_active Ceased
- 2001-03-01 AT AT01909810T patent/ATE294855T1/de active
- 2001-03-01 JP JP2001565862A patent/JP4832694B2/ja not_active Expired - Lifetime
- 2001-03-01 US US10/220,908 patent/US7507567B2/en not_active Expired - Lifetime
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