CA2400049C - Rna polymerase mutants with increased thermostability - Google Patents

Rna polymerase mutants with increased thermostability Download PDF

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Publication number
CA2400049C
CA2400049C CA2400049A CA2400049A CA2400049C CA 2400049 C CA2400049 C CA 2400049C CA 2400049 A CA2400049 A CA 2400049A CA 2400049 A CA2400049 A CA 2400049A CA 2400049 C CA2400049 C CA 2400049C
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CA
Canada
Prior art keywords
rna polymerase
rna
mutated
gene
mutant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2400049A
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English (en)
French (fr)
Other versions
CA2400049A1 (en
Inventor
Akio Sugiyama
Yoshiaki Nishiya
Bunsei Kawakami
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Biomerieux BV
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Biomerieux BV
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Application filed by Biomerieux BV filed Critical Biomerieux BV
Publication of CA2400049A1 publication Critical patent/CA2400049A1/en
Application granted granted Critical
Publication of CA2400049C publication Critical patent/CA2400049C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Heat Sensitive Colour Forming Recording (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
CA2400049A 2000-03-07 2001-03-01 Rna polymerase mutants with increased thermostability Expired - Lifetime CA2400049C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP00200787.0 2000-03-07
EP00200787 2000-03-07
PCT/EP2001/002327 WO2001066705A1 (en) 2000-03-07 2001-03-01 Rna polymerase mutants with increased thermostability

Publications (2)

Publication Number Publication Date
CA2400049A1 CA2400049A1 (en) 2001-09-13
CA2400049C true CA2400049C (en) 2010-10-05

Family

ID=8171152

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2400049A Expired - Lifetime CA2400049C (en) 2000-03-07 2001-03-01 Rna polymerase mutants with increased thermostability

Country Status (9)

Country Link
US (1) US7507567B2 (https=)
EP (1) EP1261696B1 (https=)
JP (1) JP4832694B2 (https=)
AT (1) ATE294855T1 (https=)
AU (1) AU2001237425A1 (https=)
CA (1) CA2400049C (https=)
DE (1) DE60110564T2 (https=)
ES (1) ES2242733T3 (https=)
WO (1) WO2001066705A1 (https=)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009136153A (ja) * 2007-12-03 2009-06-25 Tosoh Corp 変異型ｒｎａポリメラーゼ
WO2009113718A1 (ja) * 2008-03-11 2009-09-17 東ソー株式会社 機能改善したｒｎａポリメラーゼ変異体
CN102177236B (zh) * 2008-08-08 2013-11-06 东曹株式会社 功能改善的rna聚合酶突变体
US9193959B2 (en) * 2010-04-16 2015-11-24 Roche Diagnostics Operations, Inc. T7 RNA polymerase variants with enhanced thermostability
CA2809284C (en) 2010-08-31 2021-02-23 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
US9062292B2 (en) 2010-09-13 2015-06-23 Enzo Life Sciences Inc. Mutant T7 polymerases
FR2981088B1 (fr) 2011-10-06 2013-11-29 Biomerieux Sa Arn polymerases mutees
US9045740B2 (en) * 2012-02-24 2015-06-02 University Of Massachusetts Modified T7-related RNA polymerases and methods of use thereof
BR112016002494A2 (pt) 2013-08-05 2017-09-05 Greenlight Biosciences Inc Proteí-nas construídas com um sítio de clivagem de protease, ácido nucléico, vetor, célula e processo de engenharia de uma proteína recombinante e de um grande número de variantes de ácidos nucleicos que codificam proteínas recombinantes
WO2015024017A2 (en) * 2013-08-16 2015-02-19 President And Fellows Of Harvard College Rna polymerase, methods of purification and methods of use
SG11201607720XA (en) * 2014-03-20 2016-10-28 Univ Texas T7 rna polymerase variants with expanded substrate range and enhanced transcriptional yield
KR20180002636A (ko) 2015-03-30 2018-01-08 그린라이트 바이오사이언시스, 아이엔씨. 리보핵산의 무세포 생산
US20190017100A1 (en) * 2015-07-01 2019-01-17 Curevac Ag Method for analysis of an rna molecule
EP3882343A3 (en) 2016-01-13 2021-12-08 New England Biolabs, Inc. Thermostable variants of t7 rna polymerase
CR20180525A (es) 2016-04-06 2019-02-14 Greenlight Biosciences Inc Producción de ácido ribonucleico libre de células
US10034951B1 (en) 2017-06-21 2018-07-31 New England Biolabs, Inc. Use of thermostable RNA polymerases to produce RNAs having reduced immunogenicity
US10793841B2 (en) 2017-06-30 2020-10-06 Codexis, Inc. T7 RNA polymerase variants
CN111417724A (zh) 2017-06-30 2020-07-14 科德克希思公司 T7 rna聚合酶变体
BR112020007068A2 (pt) 2017-10-11 2020-10-06 Greenlight Biosciences, Inc. métodos e composições para produção de nucleosídeo trifosfato e ácido ribonucleico
JP2021520815A (ja) * 2018-04-10 2021-08-26 グリーンライト バイオサイエンシーズ インコーポレーテッドＧｒｅｅｎｌｉｇｈｔ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． Ｔ７ｒｎａポリメラーゼバリアント
EP3976770A1 (en) * 2019-05-24 2022-04-06 Primordial Genetics Inc. Methods and compositions for manufacturing polynucleotides
CN112980811B (zh) * 2021-03-06 2022-02-25 苏州瀚源新酶生物科技有限公司 Rna聚合酶突变体及其应用、重组载体及其制备方法和应用、重组工程菌及其应用
EP4108770A1 (en) 2021-06-24 2022-12-28 Aptamimetics GmbH Age-selex method for aptamer identification and use
WO2025127053A1 (ja) * 2023-12-12 2025-06-19 タカラバイオ株式会社 Ｒｎａポリメラーゼ変異体とｒｎａ合成

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4952496A (en) 1984-03-30 1990-08-28 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5385834A (en) 1993-08-13 1995-01-31 Georgia Tech Research Corporation Mutant T7 RNA polymerase GP1(lys222) exhibiting altered promoter recognition
FR2761695B1 (fr) 1997-04-04 1999-09-24 Bio Merieux Arn polymerase fonctionnant preferentiellement sur matrice d'arn, sa preparation et son utilisation
JP3172710B2 (ja) * 1997-07-07 2001-06-04 理化学研究所 Ｒｎａポリメラーゼ
CA2353792C (en) 1998-12-11 2007-11-27 Akzo Nobel N.V. Mutants of rna polymerases with increased stability
JP4399684B2 (ja) * 1999-08-17 2010-01-20 東洋紡績株式会社 改良されたｒｎａポリメラーゼ

Also Published As

Publication number Publication date
EP1261696A1 (en) 2002-12-04
JP2003525627A (ja) 2003-09-02
ES2242733T3 (es) 2005-11-16
US7507567B2 (en) 2009-03-24
CA2400049A1 (en) 2001-09-13
WO2001066705A1 (en) 2001-09-13
US20030175738A1 (en) 2003-09-18
ATE294855T1 (de) 2005-05-15
DE60110564T2 (de) 2006-01-19
JP4832694B2 (ja) 2011-12-07
DE60110564D1 (en) 2005-06-09
EP1261696B1 (en) 2005-05-04
AU2001237425A1 (en) 2001-09-17

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