AU2001237425A1 - Rna polymerase mutants with increased thermostability - Google Patents

Rna polymerase mutants with increased thermostability

Info

Publication number
AU2001237425A1
AU2001237425A1 AU2001237425A AU3742501A AU2001237425A1 AU 2001237425 A1 AU2001237425 A1 AU 2001237425A1 AU 2001237425 A AU2001237425 A AU 2001237425A AU 3742501 A AU3742501 A AU 3742501A AU 2001237425 A1 AU2001237425 A1 AU 2001237425A1
Authority
AU
Australia
Prior art keywords
rna polymerase
increased thermostability
polymerase mutants
rna polymerases
bacteriophages
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001237425A
Other languages
English (en)
Inventor
Bunsei Kawakami
Yoshiaki Nishiya
Akio Sugiyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Akzo Nobel NV
Original Assignee
Akzo Nobel NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Akzo Nobel NV filed Critical Akzo Nobel NV
Publication of AU2001237425A1 publication Critical patent/AU2001237425A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Heat Sensitive Colour Forming Recording (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
AU2001237425A 2000-03-07 2001-03-01 Rna polymerase mutants with increased thermostability Abandoned AU2001237425A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP00200787 2000-03-07
EP00200787 2000-03-07
PCT/EP2001/002327 WO2001066705A1 (en) 2000-03-07 2001-03-01 Rna polymerase mutants with increased thermostability

Publications (1)

Publication Number Publication Date
AU2001237425A1 true AU2001237425A1 (en) 2001-09-17

Family

ID=8171152

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001237425A Abandoned AU2001237425A1 (en) 2000-03-07 2001-03-01 Rna polymerase mutants with increased thermostability

Country Status (9)

Country Link
US (1) US7507567B2 (https=)
EP (1) EP1261696B1 (https=)
JP (1) JP4832694B2 (https=)
AT (1) ATE294855T1 (https=)
AU (1) AU2001237425A1 (https=)
CA (1) CA2400049C (https=)
DE (1) DE60110564T2 (https=)
ES (1) ES2242733T3 (https=)
WO (1) WO2001066705A1 (https=)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009136153A (ja) * 2007-12-03 2009-06-25 Tosoh Corp 変異型ｒｎａポリメラーゼ
WO2009113718A1 (ja) * 2008-03-11 2009-09-17 東ソー株式会社 機能改善したｒｎａポリメラーゼ変異体
CN102177236B (zh) * 2008-08-08 2013-11-06 东曹株式会社 功能改善的rna聚合酶突变体
US9193959B2 (en) * 2010-04-16 2015-11-24 Roche Diagnostics Operations, Inc. T7 RNA polymerase variants with enhanced thermostability
CA2809284C (en) 2010-08-31 2021-02-23 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
US9062292B2 (en) 2010-09-13 2015-06-23 Enzo Life Sciences Inc. Mutant T7 polymerases
FR2981088B1 (fr) 2011-10-06 2013-11-29 Biomerieux Sa Arn polymerases mutees
US9045740B2 (en) * 2012-02-24 2015-06-02 University Of Massachusetts Modified T7-related RNA polymerases and methods of use thereof
BR112016002494A2 (pt) 2013-08-05 2017-09-05 Greenlight Biosciences Inc Proteí-nas construídas com um sítio de clivagem de protease, ácido nucléico, vetor, célula e processo de engenharia de uma proteína recombinante e de um grande número de variantes de ácidos nucleicos que codificam proteínas recombinantes
WO2015024017A2 (en) * 2013-08-16 2015-02-19 President And Fellows Of Harvard College Rna polymerase, methods of purification and methods of use
SG11201607720XA (en) * 2014-03-20 2016-10-28 Univ Texas T7 rna polymerase variants with expanded substrate range and enhanced transcriptional yield
KR20180002636A (ko) 2015-03-30 2018-01-08 그린라이트 바이오사이언시스, 아이엔씨. 리보핵산의 무세포 생산
US20190017100A1 (en) * 2015-07-01 2019-01-17 Curevac Ag Method for analysis of an rna molecule
EP3882343A3 (en) 2016-01-13 2021-12-08 New England Biolabs, Inc. Thermostable variants of t7 rna polymerase
CR20180525A (es) 2016-04-06 2019-02-14 Greenlight Biosciences Inc Producción de ácido ribonucleico libre de células
US10034951B1 (en) 2017-06-21 2018-07-31 New England Biolabs, Inc. Use of thermostable RNA polymerases to produce RNAs having reduced immunogenicity
US10793841B2 (en) 2017-06-30 2020-10-06 Codexis, Inc. T7 RNA polymerase variants
CN111417724A (zh) 2017-06-30 2020-07-14 科德克希思公司 T7 rna聚合酶变体
BR112020007068A2 (pt) 2017-10-11 2020-10-06 Greenlight Biosciences, Inc. métodos e composições para produção de nucleosídeo trifosfato e ácido ribonucleico
JP2021520815A (ja) * 2018-04-10 2021-08-26 グリーンライト バイオサイエンシーズ インコーポレーテッドＧｒｅｅｎｌｉｇｈｔ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． Ｔ７ｒｎａポリメラーゼバリアント
EP3976770A1 (en) * 2019-05-24 2022-04-06 Primordial Genetics Inc. Methods and compositions for manufacturing polynucleotides
CN112980811B (zh) * 2021-03-06 2022-02-25 苏州瀚源新酶生物科技有限公司 Rna聚合酶突变体及其应用、重组载体及其制备方法和应用、重组工程菌及其应用
EP4108770A1 (en) 2021-06-24 2022-12-28 Aptamimetics GmbH Age-selex method for aptamer identification and use
WO2025127053A1 (ja) * 2023-12-12 2025-06-19 タカラバイオ株式会社 Ｒｎａポリメラーゼ変異体とｒｎａ合成

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4952496A (en) 1984-03-30 1990-08-28 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5385834A (en) 1993-08-13 1995-01-31 Georgia Tech Research Corporation Mutant T7 RNA polymerase GP1(lys222) exhibiting altered promoter recognition
FR2761695B1 (fr) 1997-04-04 1999-09-24 Bio Merieux Arn polymerase fonctionnant preferentiellement sur matrice d'arn, sa preparation et son utilisation
JP3172710B2 (ja) * 1997-07-07 2001-06-04 理化学研究所 Ｒｎａポリメラーゼ
CA2353792C (en) 1998-12-11 2007-11-27 Akzo Nobel N.V. Mutants of rna polymerases with increased stability
JP4399684B2 (ja) * 1999-08-17 2010-01-20 東洋紡績株式会社 改良されたｒｎａポリメラーゼ

Also Published As

Publication number Publication date
EP1261696A1 (en) 2002-12-04
JP2003525627A (ja) 2003-09-02
ES2242733T3 (es) 2005-11-16
CA2400049C (en) 2010-10-05
US7507567B2 (en) 2009-03-24
CA2400049A1 (en) 2001-09-13
WO2001066705A1 (en) 2001-09-13
US20030175738A1 (en) 2003-09-18
ATE294855T1 (de) 2005-05-15
DE60110564T2 (de) 2006-01-19
JP4832694B2 (ja) 2011-12-07
DE60110564D1 (en) 2005-06-09
EP1261696B1 (en) 2005-05-04

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