JP2003342120A - Skin care preparation - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a skin care preparation which gives a high moisture- retaining effect and synergistically improving effects for skin-aged symptoms such as wrinkles and for skin roughness. <P>SOLUTION: This skin care preparation contains the extract of Chamaenerion angustifolium (L.) Scop. or Epilobium angustifolium L., and one or more conventional cell activators selected from various galenicals, plant extracts, ceramide, yeast extracts, nucleic acids, and hydroxyfatty acids. The skin care preparation gives the excellent moisture-retaining effect and the synergistically improved effects for the skin-aged symptoms such as the wrinkles and for the skin roughness. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention has a high moisturizing effect,
The present invention relates to a skin external preparation which synergistically improves the effects of improving skin aging symptoms such as wrinkles and the effect of improving rough skin. More specifically, the present invention relates to an external preparation for skin, which is prepared by using an extract of a Japanese algae and one or more kinds selected from cell activating agents in combination.

[0002]

2. Description of the Related Art Conventionally, due to aging, exposure to ultraviolet rays, etc., the stratum corneum of the skin, epidermal cells and dermal cells are damaged, the number of cells in the epidermis is reduced, the metabolism of epidermal cells is reduced, and the skin turns. It is known that the overspeed becomes slow, the hyaluronic acid is reduced in the dermis, and collagen, elastin and the like are denatured, causing skin aging and rough skin symptoms such as wrinkle formation and elasticity reduction. In order to prevent or improve these processes, many skin external preparations have been proposed. Recently, reflecting the nature-oriented and plant-oriented, or in the plant extract components, components that suppress the decomposition of collagen and elastin, which is a constituent of the dermal matrix, hyaluronic acid, etc., or promote their production,
The search for components that activate epidermal cells and dermal fibroblasts is being actively conducted.

For example, as a component for activating epidermal cells, a parsley extract and a beech tree extract are used in combination (Japanese Patent Application Laid-Open No. H11 (1999) -111945).
-335257) and the like are disclosed. In addition, loquat extract (Japanese Patent Publication No. 5-17206) and α-hydroxyacetic acid (Japanese Patent Laid-Open No. 5-206206) are used as agents for activating or proliferating fibroblasts.
112422), sterol ester of α-hydroxy acid (JP-A-8-104632), 6-benzylaminopurine (JP-A-7-233037), specific ribonuclease (JP-A-7-309778), L-lysyl-L-glycyl-
L-histidine (JP-A-7-316192), milk-derived fibroblast growth factor (JP-A-8-119867), oxidized coenzyme A (JP-A-8-175961), almond, dandelion, Sambucus nigra, senkyu, senburi, soakuhakuhi. , Tounin, carrot, hops, syringa, yokinin (JP-A-10-36279), a silicic acid-related substance (JP-A-7-188036) as a collagen metabolism-improving agent, and glycine, proline, alanine as a collagen synthesis promoter. Combination system (JP-A-7-
194375) is a hyaluronic acid production promoter,
Low molecular weight fraction of bovine serum having a molecular weight of 5,000 or less (JP-A-8-239404), yeast extract (JP-A-8-1639)
83) and the like are disclosed.

However, among the above-mentioned components having a cell activating effect, the action and effect are insufficient, and the stability is poor, so that it is effective when contained in the skin external preparation base. Some have had to contain significant amounts to be effective. In addition, it has unfavorable side effects or irritation, adversely affects the stability of the formulation, is difficult to mix with external preparations in terms of odor or color, and it is difficult to maintain a certain action and quality. There were many things.

[0005]

Therefore, in the present invention, it is an object of the present invention to obtain a skin external preparation having a high moisturizing effect and synergistically improving the skin aging symptoms such as wrinkles and the rough skin improving effect. did.

[0006]

[Means for Solving the Problems] Various investigations were carried out to solve the above problems.
Excellent moisturizing effect by including one or more kinds selected from commonly used cell activating agents such as plant extract, ceramide, yeast extract, nucleic acids and hydroxy fatty acids in combination in the external preparation for skin. It was found that the effect of improving skin aging symptoms such as wrinkles and the effect of improving rough skin can be obtained synergistically, and the present invention has been completed.

[0007] It should be noted, Koukaishi is a kind of herbal medicine, onagraceae (Onagraceae) of the plant Yanagiran (Ch
amaenerion angustifolium
(L.) Scop. Or Epilobium
angustifolium L. ) Based on the whole plant. Its leaves contain tannin, ursolic acid, oleanolic acid, etc. and are known to have anti-inflammatory effects.

The cell activating agent has been blended in an external preparation for skin for a long time in anticipation of the effect of improving rough skin, and the present invention obtained by using a Koukai extract and a cell activating agent in combination. The effect of has never been suggested.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION In the present invention, it is convenient to use a commercially available crude drug "Koukaishi". In addition, one or more species selected from each part such as the flower, fruit, stem, leaf, root, etc. of the willow plant, which is the basic plant of Koukaishi, or whole grass can be used, and in that case, it is extracted as raw Although it may be subjected to an operation, in consideration of extraction efficiency, it is preferable to perform extraction after performing processing such as shredding, drying and crushing. The extraction is performed by immersing it in an extraction solvent. It may be stirred or homogenized in an extraction solvent in order to improve the extraction efficiency.
As the extraction temperature, it is appropriate to set the temperature to about 5 ° C. to a temperature not higher than the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is suitable to be about 4 hours to 14 days.

As the extraction solvent, in addition to water, lower alcohols such as methanol, ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, diethyl ether and dipropyl. Polar organic solvents such as ethers such as ethers, esters such as ethyl acetate and butyl acetate, ketones such as acetone and ethyl methyl ketone are preferably used, and one or more kinds thereof are selected and used. Alternatively, physiological saline, phosphate buffer, phosphate buffered saline, etc. may be used.

The extraction solvent is not particularly limited as long as it is the above solvent, but a polar solvent is preferable, and further, ethanol,
One or two or more kinds of mixed solvents selected from 1,3-butylene glycol and water are preferable, and among them, a 50% by volume aqueous ethanol solution is most preferable as the extraction solvent.

The extract of Koukaishi from the above-mentioned solvent can be used as it is, but the concentrated and dried product is redissolved in water or a polar solvent, or decolorized, deodorized and deodorized within a range not impairing its action. It may be used after purification treatment or fractionation treatment of salt or the like. Also, for storage,
It is preferable to freeze-dry after the purification treatment and dissolve in a solvent before use. It can also be encapsulated in vesicles such as liposomes or microcapsules.

The amount of the above-mentioned Koukai extract to be added to the skin external preparation is generally 0.001 to 10% by weight, preferably 0.1 to 5% by weight, although it depends on the extraction method.

In the present invention, one or more kinds selected from the cell activating agents are used in combination with the above-mentioned extract of Boletus cinnamomea, to be contained in the skin external preparation base.

Examples of the cell activating agent include ceramides and ceramide-like structural substances, yeast extracts and yeast culture supernatants, crude drug extracts of cucumber, Eucommia ulmoides, litchi and amanin, plants of the genus Asparagus , avocado ( Persea americana Mill.), aloe (aloe) plants of the genus, apricot (Prunus armeniaca L. var. ansu Maxi
m.), Ginkgo biloba L., Fauna
s japonica Maxim.), garlic ( Allium sativum)
L. f. Pekinense Makino), Panax ginseng ( Panax gi )
nseng CAMeyer), chamomile ( Matricaria chamomilla )
L.), Seiyo and Cucumber ( Cucumis sativus L.),
Calendula arvensis L., ghetto ( Alp
inia speciosa (Wendl.) K. Schum.), shiitake ( Len
tinus edodes Sing.), Cinnamon (Kiwi) ( Acti
nidia chinensis Planch.), Horsetail ( Equisetum arven )
se L.), garlic ( Allium sativum L.), yarrow ( Achillea aplina L .; Achillea
sibilica Ledeb.), assembly (Swertia japonica Maki
no), Stephania cepharantha Hay
ata), chisha (lettuce) ( Lactuca sativa L.), swallow moto ( Clintonia udensis Trautv. et Mey .; Clin
tonia borealis ), Capsicum annuum
L.), Houttuynia cordata Thunb., Horse chestnut ( Aesculus turbinata Blume), Grape ( Vitis vi
nifera L.), beech ( Fagus crenata Blume), loofah ( Luffa cylindrica M.Roemen), safflower ( Carthamus )
tinctorius L.), Mannenrou ( Rosmarinus officina )
lis L.), tangerine ( Citrus ) plant, Sapind ( Sapind)
us mukurossi Gaertn.), purple ( Lithospermum off )
icinale L. var. erythrorhizon Maxim.), Salixae plant, Eucalyptus plant, Lilium plant, Eriophorum
L.) Extracts of genus plants, hydroxy fatty acids and their salts and derivatives, nucleic acids and related substances, proteins extracted from eggshell membranes, sericin and its hydrolysates, lactic acid bacteria extracts, bifidobacteria extracts, algae extracts things, bittern components, vitamin a, vitamin B _2, vitamin B ₆ such, nicotinic acid and its salts and derivatives, ergocalciferol, cholecalciferol shea vitamin D such as fellows Le, vitamins H such, mammals such as human and bovine Animal spleen extracts, basic and acidic fibroblast growth factors, and epidermal growth factor are exemplified as preferable ones, and one or more kinds of them are selected and used.

Ceramide is an N-acyl derivative of sphingosine or a similar base thereof. In addition to natural ceramide,
Phytosphigocin-containing ceramide, N- (hexadecyloxyhydroxypropyl) -N-hydroxyethylhexadecanamide, N-acylsulfigocin-containing ceramide, synthetic ceramides such as sphingomyelin and phytosphingosine, and ceramide-like compounds is there. Further, examples of the ceramide derivative include glucosylceramide, galactosylceramide, and the like, and these ceramide derivatives can also be produced by using a generally known method.

In the present invention, generally referred to as type 1
~ Extracted from natural products such as ceramides having various structures such as type 6, for example, N-stearoyl phytosphingosine (Ceramide III manufactured by Cosmo Farm), hydroxystearyl phytosphingosine (ceramide VI manufactured by Cosmo Farm), and ceramides derived from rice bran A thing can also be used.

These ceramides and ceramide-like structural substances may be used either individually or in combination of two or more, preferably 0.0001 to 20% by weight in the total composition, particularly 0.01 to 20% by weight. It is preferable to add 10% by weight.

As the yeast extract, an extract of yeast with a polar solvent, a yeast which has been lysed by autolysis, acid hydrolysis, enzymatic decomposition, or the like, or which has been filtered, or the lysate that has been dried, and then a polar solvent can be used. The extracted one can be used. Extract the Eremascus ( Eremascus )
Genus, Endomyces ( Endomyces ) and other yeast belonging to the family Endomycetaceae ( Endomycetaceae ), Schizosaccharomyces ( Schizosaccharomyces ), Nadsonia ( Nadsoni )
a) the genus, Sakkaromikodesu (Saccharomycodes) genus, Hanseniasupora (Hanseniaspora) genus, Wicker Hami A (Wickerhamia) genus Saccharomyces (Saccharomyces)
Sp., Kluyveromyces (Kluyveromyces) genus, Rodderomisesu (Lodderomyces) genus, Wingea (Wingea) genus,
Endomikopushisu (Endomycopsis) genus Pichia (Pichi
a) sp., Hansenula (Hansenula) spp., Pakisoren (Pach
ysolen) genus, Shiteromisesu (Citeromyces) genus Debaryomyces (Debaryomyces) genus Schwanniomyces (Sc
hwanniomyces) genus, Dekkera (Dekkera) genus Saccharomyces Romi Kopu cis (Saccharomycopsis) genus, Ripomisesu (Lipo
myces ) Saccharomycetacea ( Saccharomycetacea
e ) Yeast belonging to the family, Spermophthora
Genus, Eremoteshiumu (Eremothecium) genus, Kureburoteshiumu (Crebrothecium) genus, Ashubuya (Ashbya) genus,
Nematosupora (Nemat ospora) genus, meth Gerhard Nico Weir (Metschnikowia) genus, flag Diaz box (Coccidias
cus ) spermophthoraceae ( Spermophthoraceae )
Ascomycete yeast such as yeast belonging to the family is preferably used.

As the extraction solvent, in addition to water, lower alcohols such as methanol, ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, diethyl ether and dipropyl. It is possible to use ethers such as ether, esters such as ethyl acetate and butyl acetate, and polar organic solvents such as ketones such as acetone and ethyl methyl ketone.
One kind or two or more kinds are selected and used. Also, saline
Phosphate buffer, phosphate buffered saline, etc. may be used. The yeast may be freeze-dried and / or pulverized before being subjected to extraction, or may be homogenized in an extraction solvent or ultrasonically disrupted. It is also possible to irradiate the medium with ultraviolet rays to obtain an extract. The extraction temperature is suitably about 0 ° C. to a temperature not higher than the boiling point of the extraction solvent. The extraction time varies depending on the type of the extraction solvent and the extraction temperature, but it is suitable to be about 1 hour to 5 days.

The yeast culture supernatant is cultured at 15 ° C. to 28 ° C. for about 3 days to 4 weeks using a yeast culture medium such as MY medium, then the culture supernatant is collected and filtered through a membrane filter. Get it.

The extract or culture supernatant of the above-mentioned polar solvent of yeast can be contained in the external preparation for skin according to the present invention as it is, but it can be concentrated and dried, or the concentrated and dried solid can be mixed with water or It may be used after being redissolved in a polar solvent, or subjected to a purification treatment such as decolorization, deodorization, desalting or the like or a dispersion treatment within a range that does not impair the physiological action. Also for storage,
It is also possible to freeze-dry after the purification treatment and dissolve in a solvent before use. It can also be contained in a vesicle such as a liposome or a microcapsule.

The yeast extract or culture supernatant may be prepared as described above, or may be commercially available as "yeast extract" for pharmaceuticals and cosmetics. In the present invention, one or more selected from these yeast extract and yeast culture supernatant are used.
The content per total amount of the external preparation for skin is not particularly limited, but is preferably about 0.0001 to 10.0% by weight.

[0024] The herbal Kakkonekisu (Puerariae Radix) is a perennial plant belonging to the legume family (Leguminosae) kudzu (Puera
ria lobata Ohwi) root excluding the pericardium, made in Japan,
You can use Korean and Chinese square brackets, board brackets and powder brackets.

The herb Medicinal herb is parasitic on the insects of the order Lepidoptera and Coleoptera or its larvae to form a sclerotia in the body, and in the summer the offspring formed on the body surface of the insect or its larvae. It is an entity, Cordyceps sinensis , and semi-bamboo Cordycep s sobo
lifera B.), Pleurotus ostreatus ( Cordyceps militaris Lin)
k), Mimitake mushroom ( Cordyceps nutans Pat.) and the like can be used.

The herbal medicine Ganoderma lucidum ( Ganoderm)
Ganod ( Ganod ), a basidiomycete belonging to Ataceae
erma lucidum (Leyss. ex Fr.) Karst) is a dried fruiting body, and white sardine lacquer , Kazuno lacquer, etc. can be used.

The herbal medicine Alinin ( Lini Semen ) is an annual herb of Linaceae, Linum usitatis.
imum L.) seeds.

[0028] asparagus (Asparagus) plant of the genus is a perennial belonging to the Liliaceae (Liliaceae), which is MotoHara plant of herbal "Tenmondou" Kusasugikazura (Asparagus co
chinchinensis Merrill) In addition, willow batten Monde cormorant (Asparagus falcatus L.), asparagus asparagoides (Aspar
agus medeoloides Thunb.), Tachiten Mondow ( Aspar
agus myriocladus Hort.), Dutch Pheasant Beetle ( Aspa
ragus officinalis L.) etc. are illustrated. Flowers, leaves, stems,
Although various parts such as roots and fruits can be used, it is preferable to use roots or stems and foliar branches.

Avocado ( Persea americana Mill.)
A tree belonging to the Lauraceae family, which can use various parts such as flowers, leaves, branch trunks, and fruits, but preferably fruits.

The plant of the genus Aloe belongs to the family Liliac.
eae ) is a woody succulent plant and is a herbal medicine "Aloe" ( Alo
e ) Used as a base plant. Aloe Ferokusu (Aloe ferox Mill.), Aloe Africana (Aloe a
fricana Mill.), Aloe spicata Bake
r), Aloe succotrina Lam.,
Aloe plicatilis Mill.,
Aloe bainesii Th. Dyer., Aloe marlothii Bgr., Aloe
Perry ( Aloe perryi Baker), Aloe Vera ( Aloe ve)
ra L.), Aloe barbadensis Mil
l.) and Kidachi Aloe ( Aloe arborescens Mill., Aloe
arborescens Mill. var. natalensis Berg.) and the like, and each part of leaves, flower stems, flowers and the like and the whole plant can be used, but it is preferable to use the leaf part.

Apricot ( Prunus armeniaca L. var. Ansu
Maxim.) Is a deciduous small tree belonging to the family Rosaceae , and can use various parts such as flowers, leaves, branches, fruits, and seeds, but it is preferable to use fruits and seeds.

Ginkgo biloba L. is a dioecious deciduous tree belonging to the Ginkgoaceae family.
Each part of leaves, branches, bark, flowers, seeds, and the whole tree can be used, but it is preferable to use leaves or seeds.

The dog beech ( Fagus japonica Maxim.) And the beech ( Fagus crenata Blume) belong to the family Beech ( Fagaceae ).
In the deciduous tree belonging to the above category, leaves, branches, bark, flowers, fruits and the like, and whole trees can be used, but it is preferable to use leaves or bark or shoots.

Allium sativum L. f. Pek
inense Makino) and garlic ( Allium sativu)
m L.) is a perennial plant belonging to the lily family ( Liliaceae ) and is a basic plant of the herbal medicine “Taisan ( Allii Bulbus )”.
Although various parts such as leaves, stems, and stems can be used, it is preferable to use stems.

Panax ginseng CA Me
yer) is a perennial belonging to Araliaceae ,
It is the base plant of the herbal medicine " Ginseng Radix ". Various parts such as flowers, stems, leaves, roots can be used,
It is preferable to use roots.

[0036] Chamomile ( Matricaria chamomilla L.)
Is an annual or perennial plant belonging to the family Asteraceae ( Compositae ), and is a basic plant of the herbal medicine "Chamomillae Flos ". Each part of leaves, stems, roots, head flowers and the like and the whole plant can be used, but it is preferable to use head flowers.

Cucumber ( Cucumis sativus L.) is an annual vine plant belonging to the family Cucurbitaceae and has leaves, stems,
Although it is possible to use each part of flowers, fruits, etc. and whole grass,
Preference is given to using fruits.

Calendula arvensis L.
Is a first-year or second-year herb belonging to the family Compositae , and can be used for each part of leaves, stems, flowers, roots, and the whole plant, but it is preferable to use flowers.

The ghetto ( Alpinia) used in the present invention
speciosa (Wendl.) K. Schum.) is a ginger family ( Zing
As a perennial plant belonging to iberaceae ), each part of leaves, stems, flowers, roots and the like can be used, but it is preferable to use leaves or flowers.

Lentinus edodes Sing.
Mushrooms belonging to the Agaricaceae family,
Stems and whole can be used.

Cinnamon Pear (Kiwi) ( Actinidia chin
ensis Planch.) is an evergreen vine plant belonging to the Actinidiaceae family, and its various parts such as flowers, leaves, stems and fruits can be used, but it is preferable to use fruits.

Horsetail ( Equisetum arvense L.) is a perennial fern belonging to the family Equisetaceae , and can be used for each part of rhizome, spores, vegetative stem, and whole plant, but whole plant can be used. preferable.

Achillea aplina
L.; Achillea sibilica Ledeb.) Is an Asteraceae ( Composi
Tae ) is a perennial plant that can be used in various parts such as flowers, leaves and stems, but it is preferable to use whole plants.

Assembly ( Swirtia japonica Makino)
Is a perennial plant belonging to the Gentianaceae family and is the base plant of the herbal medicine " Sewrtiae Herba ".
Although various parts such as flowers, leaves and stems can be used, it is preferable to use whole grass.

[0045] Stephania cepharan
tha Hayata) is a perennial vine plant belonging to the family Menispermaceae , and can be used for each part of leaves, flowers, stems, roots, seeds, etc., but it is preferable to use roots.

Chisha (lettuce) ( Lactuca sativa L.)
Is a perennial herb belonging to the family Asteraceae ( Compositae ).
Although various parts such as leaves and stems can be used, it is preferable to use leaves.

Swallow Omoto ( Clintonia udensis Traut
v. et Mey.; Clintonia borealis ) is a lily family ( Lilia
ceae ) is a perennial herb that is the base plant of the herbal medicine " Raikou Nana". Each part such as flower, leaf, stem can be used,
It is preferable to use leaves.

Capsicum annuum L. is a shrub-like woody perennial plant belonging to the Solanaceae family, and is the base plant of the herb " Capsici Fructus ". Although various parts such as flowers, leaves, stems and fruits can be used, it is preferable to use fruits.

Houttuynia cordata Thunb.
Is a perennial plant belonging to the family Saururaceae and is the base plant of the herbal medicine " Houttuyniae Herba ". Each part such as flower, leaf, stem can be used,
It is preferable to use whole plants before flowering.

Grape ( Vitis vinifera L.) is a deciduous vine plant belonging to the family Vitaceae , and includes flowers, leaves,
Although various parts such as stems, fruits and seeds can be used, it is preferable to use seeds.

Luffa cylindrica M. Roemen
Is an annual vine plant belonging to the family Cucurbitaceae, which can use flowers, leaves, stems, fruits, fruit juices, sap, etc., but preferably stems or fruits, fruit juices, sap .

Safflower ( Carthamus tinctorius L.)
Is an annual or perennial plant belonging to the family Asteraceae ( Compositae ), and is a basic plant of the herbal medicine " Carhami Flos ". Although various parts such as flowers, leaves, stems and seeds can be used, it is preferable to use flowers.

Mannenrou ( Rosmarinus officinalis
L.) is an evergreen shrub belonging to Labiatae and has leaves,
Each part of the branch, bark, flower, etc. and the whole tree can be used, but it is preferable to use leaves.

Citrus plants belong to the Rutaceae family ( Ru
taceae ) is an evergreen fruit tree that belongs to the lime ( Citrus auranti
folia Swingle), Daidai ( Citrus aurantium L.),
Bergamot ( Citrus bergamia Risso et Poit.), Lemon ( Citrus limon Burm.), Natsumikan ( Citrus nat )
sudaidai Hayata), sweet orange ( Citrus sinens
is Osbeck), Navel Orange ( Citrus sinensis Osb
eck var. brasiliensis Tanaka), Cabos ( Citrus sph )
aerocarpa Hort. ex Tanaka), Unshu mikan ( Cit
rus unshiu Marcovitch) and the like are exemplified as preferable ones. Although various parts such as flowers, leaves, stems, fruits, and peels can be used, it is preferable to use leaves, fruits, or peels.

Mukuroji ( Sapindus mukurossi Gaertn.)
Is a deciduous tree belonging to the family Sapindaceae ,
Although various parts such as flowers, leaves, branch trunks, bark, and fruits can be used, it is preferable to use pericarp or bark.

Purple ( Lithospermum officinale L. v
ar. erythrorhizon Maxim.) is a member of the purple family ( Boragina
ceae ) is a perennial herb belonging to the herbal medicine "Sikon ( Lithospermi
Radix ) ”is the basic plant. Although various parts such as flowers, leaves, stems, and roots can be used, it is preferable to use roots.

The Salixae plants include Chosenia arbutifolia ( Chosenia arbutifolia).
(Pallas) A. Skvortz; Chosenia bracteosa Nakai),
Populus sieboldi Miq., Populus sieboldi Miq., Populus maxmowiczii Henry, Populus alba L., Populus alto L., Populus deltoides Marsh Populus monilifera
Ait .; Populus angulata Aiv.), Populu
s euphratica Oliv.), European Populus ( Popu)
lus nigra L.), Populus nigra va
r. italica Koehne), European aspen ( Populus t
remula ), Salix acutifolia, Salix acutifolia
L.), willow ( Salix alba L .; Salix aurea
Salisb.), Weeping willow ( Salix babylon )
ica L.), Wild Willow ( Salix bakk )
o Kimura), Akameyanagi (Salix chaenomeloides Kimur
a), Naga bus sallow (Salixgilgiana Seemen.), Pussy Willow (Enokoroyanagi, a sallow) (Salix gracili
styla Miq.), Inukyanagi ( Salix integra Thun)
b.), Salix japonica Thunb., Salix japonica Thunb.
( Salix kinuyanagi Kimura), Koryanagi ( Salix koriya
nagi Kimura; Salix purpurea var. japonica Nakai),
Furisodeyanagi (Salix leucopithecia Kimura), down Liu willow (Salix matsudana Koidz.), Takamine Iva willow (Salix Nakamurana) (Salix nakamurana Koidz.),
Black willow ( Salix nigra ), purple willow ( Salix purp)
urea L.), Salix sac
halinensis Fr.Schm.), Miyanagi (Mine willow) ( S
alix reinii Fr.et Sav.), Salix sieboldi
ana Bl.), Salix viminalis L; S
alix longifolia Lam.), Fox willow
( Salix vulpina ), Salix vulpina
usu urbaniana ; Salixurbaniana ) and the like, and bark, leaves and female flowers are preferably used. Such Salixae plants contain a large amount of salicin, and this salicin may be used alone.

The Eucalyptus ( Eucalyptus ) genus plant is first described as Eucalyptus globulus Labill.
And related plants, such as the Myrtaceae family ( My
It is an evergreen tree belonging to rtaceae ) and is the base plant of the crude drug “Eucalyptus oil” ( Oleum Eucalypti ). The closely related plants, eucalyptus Poriburakutea (Eucalypt
us polybractea RT Baker), Eucalyptus dives Schauer. Others, lemon eucalyptus ( Eucalyptus citriodora Hoo
k.), Eucalyptus piperit
a Sm.), Eucalyptus gunn
ii Hook. fil.), eucalyptus Makarutsuri (Eucal
yptus macarthuri Decne. Et Maiden), Eucalyptus rostrata Schlecht.,
Eucalyptus Sutaigeriana (Eucalyptus staiger
iana F. v. M.) and the like can also be used. Each part of leaves, branches, bark, flowers, fruits and the like and whole trees can be used, but it is preferable to use leaves.

The lily ( Lilium ) genus plants include Yamali ( Lilium auratum Lindl.) And Madonna lily ( Lilium ).
candidum L.), Lilium concolor Salis
b.), Lilium formosanum Wallace,
Sasayuri (Lilium japonicum Thunb. Ex Houtt. ), Tiger Lilies (Lilium lancifolium Thunb.), Kooniyuri (L
ilium leichtlinii Hook. f. var.maximowiczii Bake
r), lily (Liliumlongiflorum Thunb.), Iwatoyuri (Lilium maculatum Thunb.), Lilium Medeoloides (L
ilium medeoloides A. Gray), legal lily ( Liliu
m regale Wils.), Lilium rebellum Bake
r), Lilium speciosum (Lilium speciosum Thunb.), and the like. These are perennials belonging to the family Liliaceae , and can be used for each part of leaves, stems, flowers, bulbs and the like, and it is preferable to use bulbs.

Examples of plants belonging to the genus Watari ( Eriophorum L.) include herring ( Eriophorum gracil Koch subsp. Corean) .
um (Palla) T. Koyama; Eriophorum coreanum Pall
a), cotton boll ( Eriophorum vaginatum L. subsp. faur
iei (EG Camus) T. Koyama; Eriophorum faurei E.
G. Camus; Scirpus faurei (EG Camu) T. Koyam
a), Takanekurosuge (Eriophorum japonicum), Ezowatasuge (Eriophorum scheuchzerii var. tenuifoliu
m ), Chihuahua sedge ( Eriophorum scheuchzerii var. s .
cheuchzerii ) and the like. These are the perennial belonging to the Cyperaceae (Cyperaceae), flowers, leaves, stems,
Various parts such as roots, spikelets, and fluff can be used.

The above herbal medicines and plant extracts include water,
Lower alcohols such as methanol, ethanol, propanol, isopropanol, 1,3-butylene glycol, propylene glycol, dipropylene glycol,
Polyhydric alcohols such as glycerin, ethers such as diethyl ether and dipropyl ether, esters such as ethyl acetate and butyl acetate, polar organic solvents such as ketones such as acetone and ethyl methyl ketone, or physiological saline and phosphoric acid It can be obtained using a buffer solution, a phosphate buffered saline, or the like. Furthermore, a method of extracting using a distillation method such as steam distillation, a method of pressing a plant to obtain an extract, and the like are exemplified, and these methods are used alone or in combination of two or more to perform extraction. The extract may be re-dissolved in water or a polar solvent as it is, or may be concentrated and dried to dryness, or may be subjected to purification treatments such as decolorization, deodorization and desalting within a range not impairing physiological effects thereof, and column chromatography. It can be used after performing a fractionation process by the like. In addition, commercially available raw materials for pharmaceuticals and cosmetics from various companies can also be used. The content of the external preparation for skin is preferably 0.0001 to 10.0% by weight, although it varies depending on the method for preparing the extract.

The hydroxy fatty acid has 2 to 3 carbon atoms.
A linear or branched saturated or unsaturated fatty acid having a hydroxyl group at the 2-position or 3-position of 0 is preferably used. For example, 2-hydroxyethanoic acid (glycolic acid), 2-hydroxypropanoic acid (lactic acid), 2-methyl-2-hydroxypropanoic acid (methyllactic acid), 2-hydroxybutanoic acid, 2-hydroxypentanoic acid, 2-hydroxy. Hexanoic acid, 2-hydroxyheptanoic acid, 2-hydroxyoctanoic acid, 2-hydroxynonanoic acid, 2-hydroxydecanoic acid, 2-hydroxyundecanoic acid, 2-
Hydroxydodecanoic acid (2-hydroxylauric acid),
2-hydroxytetradecanoic acid (2-hydroxymyristic acid), 2-hydroxyhexadecanoic acid (2-hydroxypalmitic acid), 2-hydroxyoctadecanoic acid (2-hydroxystearic acid), 2-hydroxyeicosanoic acid (2-hydroxyaraquine) Acid), 2-hydroxytetracosanoic acid (cerebronic acid), 2-hydroxy-
9-undecenoic acid (2-hydroxy-9-undecylenic acid), 2-hydroxy-10-undecenoic acid (2-hydroxy-10-undecylenic acid), 2-hydroxy-c
is-15-tetracosenoic acid (2-hydroxynervonic acid), lanolin-derived long-chain 2-hydroxy fatty acid, 3-
Hydroxypropanoic acid, 2-methyl-3-hydroxypropanoic acid, 3-hydroxybutanoic acid, 3-hydroxypentanoic acid, 3-hydroxyhexanoic acid, 3-hydroxyheptanoic acid, 3-hydroxyoctanoic acid, 3-hydroxynonanoic acid, 3-hydroxydecanoic acid, 3-hydroxyundecanoic acid, 3-hydroxydodecanoic acid (3-hydroxylauric acid), 3-hydroxytetradecanoic acid (3
-Hydroxymyristic acid), 3-hydroxyhexadecanoic acid (3-hydroxypalmitic acid), 3-hydroxyoctadecanoic acid (3-hydroxystearic acid), 3
-Hydroxyeicosanoic acid (3-hydroxyarachidic acid), 3-hydroxytetracosanoic acid, 3-hydroxy-9-undecenoic acid (3-hydroxy-9-undecylenic acid), 3-hydroxy-10-undecenoic acid (3- Hydroxy-10-undecylenic acid), and 3-hydroxy-cis-15-tetracosenoic acid (3-hydroxynervonic acid), 2,3-dihydroxypropanoic acid (glyceric acid), erythronic acid, threonic acid, and the like 2,3, 2,3,4,5-tetrahydroxypentanoic acid, such as 4-trihydroxybutanoic acid, ribbon acid, arabinonic acid, xylonic acid, lyxonic acid, alloic acid, altronic acid, gluconic acid,
2,3,4,5,6-pentahydroxyhexanoic acid such as mannonic acid, gulonic acid, idonic acid, galactonic acid and talonic acid, 2,3 such as glucoheptonic acid and galactoheptonic acid,
4,5,6,7-Hexahydroxyheptanoic acid, hydroxymethanedicarboxylic acid (tartronic acid), 1-hydroxyethane-1,2-dicarboxylic acid (malic acid), 1,2-
1,2, such as dihydroxyethane-1,2-dicarboxylic acid (tartaric acid), 2-hydroxypropane-1,2,3-tricarboxylic acid (citric acid), glucaric acid and mutic acid
3,4-tetrahydroxybutane-1,4-dicarboxylic acid and the like can be mentioned. These hydroxy fatty acid salts include alkali metal salts such as sodium salts and potassium salts, alkaline earth metal salts such as magnesium salts and calcium salts,
Examples thereof include alkanolamine salts such as ammonium salts and triethanolamine salts, and basic amino acid salts such as D-, L- or DL-arginine, lysine and histidine.
Examples of these derivatives include sterol esters such as alkyl or alkenyl esters, phosphoric acid esters, sulfuric acid esters, phosphatidyl esters, cholesteryl esters, sitosteryl esters, stigmasteryl esters, and amides. In the present invention, one or more selected from these are used. The amount of the total amount of the external preparation for skin is not particularly limited,
It is suitable to set it to about 0.0001 to 10.0% by weight.

Nucleic acid and its related substances include deoxyribonucleic acid and ribonucleic acid obtained by extraction from various tissues of animals and plants, yeast, bacteria, ribonucleotides such as adenosine, guanosine, inosine, xanthosine, cytidine, uridine and ribothymidine. Deoxyribonucleosides such as nucleosides, deoxyadenosine, deoxyguanosine, deoxyinosine, deoxyxanthosine, deoxycytidine, deoxyuridine, thymidine, adenosine monophosphate, adenosine diphosphate, adenosine triphosphate, guanosine monophosphate, guanosine diphosphate Acid, guanosine triphosphate, inosine monophosphate, inosine diphosphate, inosine triphosphate, xanthosine monophosphate, xanthosine diphosphate, xanthosine triphosphate, cytidine monophosphate, cytidine diphosphate, cytidine triphosphate Ribonucleotides such as acid, uridine monophosphate, uridine diphosphate, ribothymidine monophosphate, ribothymidine diphosphate, ribothymidine triphosphate, deoxyadenosine monophosphate, deoxyadenosine diphosphate, deoxyadenosine triphosphate, deoxy Guanosine monophosphate, deoxyguanosine diphosphate, deoxyguanosine triphosphate, deoxyinosine monophosphate, deoxyinosine diphosphate, deoxyinosine triphosphate, deoxyxanthosine diphosphate, deoxyxanthosine diphosphate, deoxy Xanthosine triphosphate, deoxycytidine monophosphate, deoxycytidine diphosphate, deoxycytidine triphosphate, deoxyuridine monophosphate, deoxyuridine diphosphate, thymidine monophosphate, thymidine diphosphate,
Deoxyribonucleotides such as thymidine triphosphate, cyclic adenosine monophosphate, cyclic guanosine monophosphate, cyclic cytidine monophosphate, cyclic uridine monophosphate and other cyclic nucleotides, coenzyme A, nicotinamide adenine diphosphate Examples thereof include nucleotides, nicotinamide adenine dinucleotide phosphate, coenzymes such as flavin adenine dinucleotide, and salts thereof, and one or more of them are selected and used. The blending amount per total amount of the skin external preparation is not particularly limited, but it is suitable to be about 0.0001 to 10.0% by weight.

As the protein extracted from the eggshell membrane,
As the eggshell membrane, a membrane attached to the inside of the eggshell of birds such as chicken eggs and quail is used, and extracted from these with one or more of water and an organic solvent, or solubilization or treatment with acid, alkali or enzyme treatment. What is obtained by hydrolysis is used. A commercially available product may be used as a raw material for cosmetics. The blending amount per total amount of the skin external preparation is not particularly limited, but it is suitable to be about 0.0001 to 10.0% by weight.

As sericin and its hydrolyzate, those obtained by partially hydrolyzing sericin contained in silkworm cocoons or raw silk in an alkaline aqueous solution or by enzymatic treatment to be eluted can be used. The eluate is adjusted to pH 3 to 5 with an organic acid or an inorganic acid, or mixed with a water-soluble organic solvent such as methanol, ethanol or dioxane to precipitate a sericin hydrolyzate, which is filtered and dried to obtain a powder. It can also be used. Furthermore, sericin and its hydrolyzate used in the present invention have an average molecular weight of about 10,000.
What is -20,000, and what contains 20-40 mol% of serine is especially preferable. The blending amount per total amount of the skin external preparation is not particularly limited, but it is suitable to be about 0.0001 to 10.0% by weight.

The lactic acid bacterium extract and the bifidobacteria extract used in the present invention are not particularly limited as long as they can be used in ordinary skin external preparations. The total amount of the external preparation for skin is not particularly limited, but is 0.00
It is suitable to set the content to about 01 to 10.0% by weight.

The algae extract used in the present invention is not particularly limited as long as it is an algae extract that is used in ordinary skin external preparations. The algae for obtaining an extract, green algae, brown algae, alone one selected from red algae, or two or more are used, Yahazugusa genus among them (Di
ctyopteris ), Genus Honda Walla ( Sargassum ), Genus Fukulinami ( Dilophus ), Genus Fukuronori ( Colpomenia ),
Laminaria , Ceratodicty
on ), Rhododendron ( Asparagopsis ), Rhododendron ( Po
lysiphonia ), Salix genus ( Chondria ), Sozo ( La )
urencia ), Rattle ( Galaxaura ), Quitoceros
( Chaetoceros ), it is preferable to use an extract of one or more algae selected from the genus Pavlova .

Algae of the genus Dictyopteris are
Brown alga A kind of alga in the family Astragaliaceae.
The algae of the genus Narcissus include Dictyopter
is latiuscula ), Dwarf seaweed ( Dictyopteris undulat
a ), Herayahazu ( Dictyopteris prolifera ), Suzyahazu ( Dictyopteris plagiogramma ), Himeyahazu ( Dic
tyopteris repens ), Ezohahaz ( Dictyopteris divar )
icata ), Uraboshijahazu ( Dictyopteris polypodioide
s ) etc. are illustrated. Among these algae of the genus Astragalus, from the viewpoint of the dermal fibroblast activating action, an extract of Ulaboshihahazu ( Dictyopteris polypodioides ) is preferably used.

The algae of the genus Sargassum include Sargassum fulvellum , pea, Sargassum yendoi , and Sargassum pilu.
riferum ), Yatumatamoku ( Sargassum patens ), Akamoku ( Sargassum horneri ), Sawmillimoku ( Sargassum s )
erratifolium ), Scutellaria baicalensis ( Sargassum giga)
nteifolium), Yoremoku (Sargassum tortile), Yanagimoku (Oobamoku: Sargassum ringgoldianum), Nejimoku (Sargassum sagamianum), Hahakimoku (Sargas
sum kjellmanianum ), sea turtle ( Sargassum thunb )
ergii ), Fusijimoku ( Sargassum confusum ), Isomok ( Sargassum hemiphyllum ), Narasamo ( Sargassum )
nigrifolium), Togemoku (Sargassum micracanthu
m ), Tamanashimoku ( Sargassum nipponicum ), Jin Meso ( Sargassum vulgare ), Futaemoku (Hiragimoku: Sargassum duplicatum ), Ezononemoku ( Sargas )
sum yezoense ) etc. are illustrated. Among these algae of the genus Astragalus, in terms of the dermal fibroblast activating action, pea ( Sargassum yendoi ) and ezononejimoku ( Sargas )
sum yezoense ), and Yasumatamoku ( Sargassum paten)
It is preferable to use one or more extracts selected from s ).

Algae of the genus Dilophus are
Brown alga A kind of alga in the family Astragaliaceae.
The algae of the genus Fuchlinamidii include Fuchlinamidii ( Di
lophus okamurai , Dilophus marginata ) are exemplified.

The algae of the genus Colpomenia is one of the algae of the brown alga Cyamonolidae. The algae of the genus Fukuronori include the Fukuronori ( Colpomenia s
inuosa ) and cottonweed ( Colpomenia bullosa ). Among these algae, it is preferable to use the extract of Fukuronori ( Colpomenia sinuosa) from the viewpoint of dermal fibroblast activation activity.

The algae of the genus Laminaria is one of the algae of the family Astragalus of the order Astragalus. The algae Laminaria spp, Laminaria japonica (Laminaria japonica), Hosomekonbu (Laminaria religiosa), Mitsuishikonbu (Lamin
aria angusta ), Japanese kelp ( Laminaria cichorioid )
es ), Himekonbu ( Laminaria longipes ) and the like. Among these kelp algae, from the point of dermal fibroblast activating action, Laminaria religios ( Laminaria religios)
Preference is given to using the extract of a ).

The algae of the genus Ceratodictyon is one of the algae of the red alga Cepharidaceae Ogonoridae ,
An example is Kamensou ( Ceratodictyon spongiosum ).

The algae of the genus Asparagopsis are
Red alga A kind of algae of the family Kaedekenori,
Kagikenori ( Asparagopsis taxiformis ), Kaginori ( A
sparagopsis hamifera ) etc. are illustrated. Among these, from the viewpoint of dermal fibroblasts activation action, Kagikenori (As
It is preferable to use an extract of Paragopsis taxiformis ).

The algae of the genus Polysiphonia is one of the algae of the red alga, Cucurbitaceae, Fujimatsu family. The algae of the genus Astragalus are Polysiphonia mor.
rowii ), Dwarf Kennori ( Polysiphonia urceolat
a ), Yellow sedge ( Polysiphonia senticulosa ), Yellow squirrel ( Polysiphonia japonica ), Black sedge ( Polysiphonia forcipata ), Red sedge ( Polysi )
phonia crassa ) etc. are illustrated. Of these algae of the genus Astragalus, it is preferable to use the extract of Polysiphonia morrowii from the viewpoint of the dermal fibroblast activation activity.

The algae of the genus Chondria is one of the algae of the red alga, Aceraceae, Fujimatsu family. The algae of the genus Willowaceae include the Yuna ( Chondria crassicauli).
s ), willow nori ( Chondria dasyphylla ), akayuna ( Chondria atropurpurea ), mothleuna ( Chondria in
tricata ), hanayanagi ( Chondria armata ), crocodile ( Chondria ryukyuensis ), and the like. Among these algae of the genus Willowaceae, it is preferable to use the extract of Yuna ( Chondria crassicaulis ) from the viewpoint of dermal fibroblast activation.

The algae of the genus Laurencia is one of the algae of the red alga, Cucurbitaceae. The algae of the genus Sozo include Sozo sp. ( Laurencia sp.) And Kurosozo ( Laur
encia intermedia ), Mitsu de Sozo ( Laurencia okamura
i ), Sozonohana ( Laurenciagrevilleana ), Oosozo ( Laurencia glandulifera ), Honeysoso ( Laurencia pi )
nnata ), Cobusoso ( Laurencia undulata ) and the like. Among these algae of the genus Sozo, it is preferable to use an extract of Cobusozo ( Laurencia undulata ) from the viewpoint of dermal fibroblast activation action.

The algae of the genus Galaxaura is one of the algae of the red alga Rhinoceros order Rattleidae. The rattle algae are Galaxaura fastigiat.
a), Hiragaragara (Galaxaura falcata), beads rattle (Galaxaura obtusata) and the like. Among these algae, it is preferable to use an extract of the rattle rattle ( Galaxaura falcata ) from the viewpoint of dermal fibroblast activation activity.

The genus Chaetoceros and the genus Pavlova are Chaetoceracea.
e ) It is a kind of diatom belonging to the family, and it is particularly preferable to use Chaetoceros gracilis .

The site from which the extract is collected from algae is not particularly limited, but it is preferably extracted from whole algae or mekabu (spore leaves or adult leaves). In addition, algae are used as raw or dried. The extraction solvent is not particularly limited, and monohydric alcohols such as water, ethanol, methanol, isopropanol, isobutanol, n-hexanol, methylamyl alcohol, 2-ethylbutanol, n-octyl alcohol, glycerin, ethylene glycol, ethylene. Glycol monomethyl ether,
Propylene glycol, propylene glycol monomethyl ether, propylene glycol monoethyl ether, triethylene glycol, 1,3-butylene glycol, hexylene glycol and other polyhydric alcohols or derivatives thereof, acetone, methyl ethyl ketone, methyl isobutyl ketone, methyl-n-propyl Examples thereof include ketones such as ketones, esters such as ethyl acetate and isopropyl acetate, ethers such as diethyl ether and diisopropyl ether. Further, a polar solvent added with an inorganic salt such as phosphate buffered saline or a solvent added with a surfactant can be used, and there is no particular limitation. The amount of such algae extract blended per total amount of the skin external preparation is not particularly limited, but it is suitable to be about 0.0001 to 10.0% by weight.

Bittern is a residual salt remaining after concentrating seawater and precipitating sodium chloride. In addition to sodium chloride, potassium chloride, magnesium sulfate, magnesium bromide, magnesium chloride, sodium sulfate, potassium bromide, calcium chloride, etc. Is a mixture of. The bittern may be added either in a state of being dissolved in the aqueous phase component or in a crystalline state such as granular form. The blending amount per total amount of the skin external preparation is not particularly limited, but it is suitable to be about 0.0001 to 10.0% by weight.

As vitamins, vitamins A such as retinol, vitamin A oil, retinol acetate, vitamin B ₂ such as riboflavin and riboflavin butyrate, vitamin B ₆ such as pyridoxine hydrochloride, nicotinic acid, nicotinamide, nicotine Nicotinic acid and its salts and derivatives such as benzyl acidate, vitamin Ds such as ergocalciferol and cholecalciferol, and vitamin H such as biotin
And the like. The blending amount per total amount of the skin external preparation is not particularly limited, but it is suitable to be about 0.0001 to 10.0% by weight.

As the spleen extract, the spleen of a fresh mammal is extracted with water and deproteinized. The amount of the total amount of the external preparation for skin is not particularly limited,
It is suitable to set it to about 0.0001 to 1.0% by weight.

Fibroblast growth factor (FGF) and epidermal growth factor (EGF) are proteins that bind to tyrosine kinase type receptors and are involved in the growth promotion of dermal fibroblasts and epidermal cells, respectively. These are those isolated from humans or mammals. The blending amount per total amount of the skin external preparation is not particularly limited, but 0.0001 to
Appropriately about 1.0% by weight.

The external preparation for skin according to the present invention can be provided in various dosage forms such as lotions, emulsions, gels, creams, ointments, powders and granules. In addition, skin cosmetics such as lotion, emulsion, cream, beauty essence, pack,
Makeup base lotion, base cosmetics such as makeup base cream, foundation of each dosage form such as milky liquid, oily, solid, etc., makeup cosmetics such as eye color, cheek color, cleansing cream, cleansing lotion, cleansing foam , It can also be provided as a skin cleansing agent such as face wash soap and body shampoo, and a hair cosmetic such as hair shampoo, hair rinse and hair treatment.

The external preparation for skin according to the present invention includes oily ingredients, surfactants, humectants, pigments, ultraviolet absorbers, antioxidants, fragrances, antibacterial agents, in addition to the extract of Boletus and cell activating agent. It is possible to include general raw materials for medicines and cosmetics such as antifungal agents and physiologically active ingredients such as anti-inflammatory agents and whitening agents.

[0087]

EXAMPLES The features of the present invention will be described in detail with reference to Examples.

[Koukai extract 1] After crushing commercially available Koukai, 30 g of 50% by volume aqueous ethanol solution 3 was crushed.
It was immersed in 00 mL and left standing at 25 ° C. for 7 days. After that, the insoluble matter was removed by filtration, the filtrate was concentrated under reduced pressure, and further freeze-dried to obtain a Koukai extract 1.

[Koukai extract 2] The whole grass of Yanagiran is dried to obtain Koukai, which is crushed and then 50
0 g was immersed in 1,000 mL of a 50% by volume aqueous ethanol solution and allowed to stand at 25 ° C. for 7 days. After that, the insoluble matter was removed by filtration, and the filtrate was concentrated under reduced pressure to dryness to obtain an extract of Koukai extract 2.

Next, the cell activating agent contained in the external preparation for skin according to the present invention will be described.

[Yeast extract] Saccharomyces cerevisiae Meyer was cultured, and 500 g of cells were autolysed to obtain a lysate, which was filtered, concentrated and freeze-dried to obtain a yeast extract.

[Yeast Culture Supernatant] Saccharomyces cerevisiae Meyer was cultured, and the culture supernatant in a steady state was aseptically filtered with a membrane filter, and the filtrate was used as the yeast culture supernatant.

[Kakkon Extract] Dry powder of Kakkon 2
Soak 00g in 1 liter of ethanol and run at 20 ° C for 7
The mixture was allowed to stand for extraction for a day, and the extract was filtered, collected, concentrated, dried, and freeze-dried to give the titled Kacon extract.

[0094] [chamomile, Kidachiaroe, each extract of rosemary] chamomile (Matricaria chamomilla L.) flower head, Kidachiaroe (Aloe arborescens Mill. Var.
natalensis Berg.) leaf, mannen wax ( Rosmarinus
officinalis L.) leaves of 500 g each are dried and crushed, immersed in 1 liter of 50% by volume ethanol aqueous solution, allowed to stand at 20 ° C. for 7 days, then filtered to collect the filtrate, and extract each of the above-mentioned plants. It was a thing.

[ Ginkgo biloba] Ginkgo bil
(Oba L.) leaves (600 g) were dried, pulverized, immersed in 1 liter of 1,3-butylene glycol, and extracted by stirring at 25 ° C. for 3 days. The extract was filtered to collect the filtrate, which was used as the title plant extract.

[ Equisetum extract] Horsetail ( Equisetum arve
600 g of whole plant ( nse L.) was finely chopped , dipped in 1 liter of 50% by volume aqueous glycerin solution, and extracted by stirring at 20 ° C. for 5 days. The extract was filtered to collect the filtrate, which was used as the title plant extract.

[Safflower Extract] Safflower ( Carhamus
tinctorius L.) 1 kg of flowers are squeezed into a plate,
Dip in 1.5 liters of ethanol and stir at 15 ℃
It was left standing for a day for extraction. The extract was filtered to collect the filtrate, which was used as the title plant extract.

[Ginseng extract] 1 kg of dried root of Panax ginseng CA Meyer was added to 5
Immerse in 1.5 liters of 0% by weight ethanol, 15 ℃
The sample was allowed to stand for 10 days for extraction. The extract was filtered to collect the filtrate, which was used as the title plant extract.

[Beech extract] “GA” from GATEFOSSE
A commercially available raw material provided under the trade name of "TULINE RC" was used. This product is beech ( Fagus crenata Blume)
It is prepared by extracting with water from the young buds.

[Eggshell Membrane Extracted Protein] An egg shell membrane hydrolyzate commercially available from QP Corporation under the trade name of "EM Protein-L" was used.

[Deoxyribonucleic acid potassium salt] Commercially available organic synthetic chemicals, trade name "DNA-K" was used.

[Algae Extract] 1,3-butylene glycol 50 of the genus Rhizopus communis, the seaweed genus Seaweed, the seaweed genus Hijirimen, and the sea urchin genus Usbaaonori sold under the trade name of "Algerex S" by Ichimaru Falcos Co., Ltd. A wt% aqueous extract was used.

[Ceramide] A ceramide derived from rice bran, which is sold by Oryza Yuka Co., Ltd. under the trade name of “Oriza ceramide”, was used.

Next, the formulations of the examples of the external preparation for skin according to the present invention will be shown.

Examples 1 to 15 and Comparative Examples 1 to 3 Emulsion (1) Cetanol 1.00 (% by weight) (2) Beeswax 0.50 (3) Vaseline 2.00 (4) Squalane 6.00 (5) Dimethylpolysiloxane 2.00 (6) Polyoxyethylene (20E.O.) sorbitan monolaurate 1.00 (7) Glycerin monostearate 1.00 (8) Glycerin 4.00 ( 9) 1,3-Butylene glycol 4.00 (10) Methyl paraoxybenzoate 0.10 (11) Sperm extract shown in Table 1 0.05 (12) Purified water Amount based on 100 (13) Carboxyvinyl Polymer (1.0% aqueous solution) 10.00 (14) Potassium hydroxide (10.0% aqueous solution) 1.00 (15) Cell activating agent shown in Table 1 Amount shown in Table 1 Production method: (1) to (7) ) Oil phase components are mixed and melted by heating.
To 75 ° C. On the other hand, mix the water phase components (8) to (12).
In that case, melt to 75 ° C. Add the oil phase to this
After emulsifying, add (13) and homogenize with a homomixer.
, Then add (14) to thicken and cool,
Add (15) at 40 ° C. and mix.

[0106]

[Table 1]

Use tests were carried out on Examples 1 to 15 and Comparative Examples 1 to 3 of the present invention, and the moisture retention and
The effects of improving skin aging symptoms and skin roughness were evaluated.

First, regarding the moisturizing property, for each sample,
Twenty female panelists in their 20s to 50s were used in the blind for 2 weeks to evaluate the moist feeling. The evaluation was carried out in five grades of "very moist", "present", "somewhat", "somewhat", and "not".
The results are shown in Table 2 by the number of panelists given each evaluation.

[0109]

[Table 2]

As is clear from Table 2, the moisturizing properties of the groups used in each Example, in which the extract of Koukai extract and the cell activator were blended in combination, were highly evaluated. Good results were obtained even when compared with the groups used in Comparative Examples 2 and 3 in which they were blended.

Next, with respect to the improvement of skin aging symptoms, for each sample, each of 20 male and female panelists in their 50s to 60s in whom such symptoms were noticeably used, was blindly used for 1 month. The change in state was observed and evaluated. Skin wrinkles, texture,
The three items of the acupuncture were selected, and wrinkles and texture were observed by taking photographs and replicas of the skin surface, and the improvement status was evaluated. The skin elasticity was evaluated by measuring the skin elasticity. The improvement status was evaluated in three stages of “improvement”, “slight improvement” and “no change”, and Table 3 shows the number of panelists who obtained each evaluation.

[0112]

[Table 3]

As is clear from Table 3, wrinkles and skin elasticity tended to be improved in each of the groups used in the comparative examples which did not contain the cell activator. In the groups used in Examples compounded as described above, more panelists recognized a clear improvement than the corresponding groups used in Comparative Examples.

The improvement of the rough skin condition was evaluated by allowing 20 female panelists each having a rough skin condition to use each sample blindly for one month. The evaluation was made in five stages: "improved", "slightly improved", "no change", "slightly worse", and "worse". The results are shown in Table 4 by the number of panelists given each evaluation.

[0115]

[Table 4]

As is clear from Table 4, in any of the groups used in Examples of the present invention in which the extract of Koukai and the cell activating agent were used in combination, remarkable improvement in rough skin was observed in all cases, and after the end of the use test, , The skin condition was improved to almost good condition. On the other hand, in the comparative example use group, although there was a case in which the skin roughness was considerably improved, the degree thereof was smaller than that of the corresponding example use group.

As described above, the skin external preparations of the examples of the present invention have an excellent moisturizing effect as compared with the conventional comparative examples.
It had the effect of improving skin aging and rough skin.

Example 16 Lotion (1) Ethanol 20.0 (wt%) (2) Polyoxyethylene (60 E.O.) hydrogenated castor oil 1.0 (3) Methyl paraoxybenzoate 0.1 ( 4) Sperm extract 1 1.0 (5) 2-hydroxyacetic acid 0.5 (6) dipropylene glycol 5.0 (7) 1,3-butylene glycol 10.0 (8) purified water Total amount to 100 Quantitative manufacturing method: (2) and (3) are added to (1) and dissolved to obtain an alcohol phase. On the other hand, (4) to (7) are sequentially dissolved in (8) to form an aqueous phase. The alcohol phase is added to the aqueous phase, and the mixture is stirred and mixed.

Example 17 Emulsion (1) Cetanol 1.0 (wt%) (2) Beeswax 0.5 (3) Vaseline 2.0 (4) Squalane 6.0 (5) Dimethylpolysiloxane 2.0 (6) Polyoxyethylene (20 E.O.) sorbitan monostearate 1.0 (7) Glyceryl monostearate 1.0 (8) Glycerin 4.0 (9) 1,3-Butylene glycol 4.0 (10) Methyl paraoxybenzoate 0.1 (11) Sperm extract 2 0.5 (12) 2-Hydroxyacetic acid 0.2 (13) Purified water Amount based on 100 (14) Carboxyvinyl polymer (1. 0% by weight aqueous solution) 10.0 (15) potassium hydroxide (10.0% by weight aqueous solution) 1.0 (16) ethanol 5.0 Production method: Mix the oil phase components (1) to (7) and heat. 75 dissolved
℃. On the other hand, the water phase components (8) to (13) are mixed and dissolved to reach 75 ° C. After adding the oil phase to this and preliminarily emulsifying, add (14) and uniformly emulsify with a homomixer, then add (15) to thicken and cool, and cool at 40 ° C. (16) Add and mix.

[Example 18] Oil-in-water cream (1) Beeswax 6.00 (% by weight) (2) Cetanol 5.00 (3) Reduced lanolin 8.00 (4) Squalane 27.50 (5) Glycyrrhetin Acid stearyl 0.05 (6) Glyceryl fatty acid ester 4.00 (7) Lipophilic glyceryl monostearate 2.00 (8) Polyoxyethylene (20 E.O.) sorbitan monostearate 5.00 (9 ) Stearyl glycyrrhetinate 0.25 (10) Retinol 0.20 (11) Propylene glycol 5.00 (12) Methyl paraoxybenzoate 0.10 (13) Sperm extract 1 1.00 (14) Purified water Total 100 Manufacturing method: The oil phase components (1) to (10) are mixed and dissolved to 75 ° C. On the other hand, (11) to (14) are mixed, dissolved and heated to 75 ° C. Next, the oil phase component is added to this aqueous phase component to pre-emulsify it, then homogenize with a homomixer and cool.

[Example 19] Oil-in-water cream (1) Beeswax 6.0 (% by weight) (2) Cetanol 5.0 (3) Reduced lanolin 8.0 (4) Squalane 27.5 (5) Glyceryl Fatty acid ester 4.0 (6) Lipophilic glyceryl monostearate 2.0 (7) Polyoxyethylene (20E.O.) sorbitan monolaurate 5.0 (8) Propylene glycol 5.0 (9) Paraoxy Methyl benzoate 0.1 (10) Koukai extract 1 0.3 (11) Purified water Amount based on 100 (12) Kujin extract 0.1 Production method: (1) to (7) oil phase components Mix and dissolve to 75 ° C. On the other hand, (9) and (10) are dissolved in (8), added to (11), mixed and dissolved, and heated to 75 ° C. Next, the oil phase component is added to the aqueous phase component to pre-emulsify it, then homogenize with a homomixer, and after cooling, (12) is added and mixed at 40 ° C.

[Example 20] Gel agent (1) Dipropylene glycol 10.0 (wt%) (2) Carboxyvinyl polymer 0.5 (3) Potassium hydroxide (10.0 wt% aqueous solution) 1.0 ( 4) Methyl paraoxybenzoate 0.1 (5) Sperm extract 2 0.2 (6) Manemow extract 0.3 (7) Purified water Amount with 100 as the total amount Manufacturing method: (7) to (1)-( 6) are added sequentially and dissolved uniformly.

[Example 21] Oil-in-water emulsion type ointment (1) White petrolatum 25.0 (% by weight) (2) Stearyl alcohol 25.0 (3) Glycerin 12.0 (4) Sodium lauryl sulfate 1.0 (5) Methyl paraoxybenzoate 0.1 (6) Purified water Amount based on total amount of 100 (7) Sperm extract 1 0.1 (8) Hydrolyzed sericin aqueous solution 1.0 Manufacturing method: (1) to (4) The oil phase components of are mixed and heated to dissolve uniformly, and the temperature is adjusted to 75 ° C. On the other hand, mix the water phase components (5) to (7),
Heat to 75 ° C. The oil phase component was gradually added to this water phase component while stirring to emulsify and cool the mixture.
Add (8) at ℃ and mix.

[Example 22] Liposome agent (1) Glycerin 2.0 (% by weight) (2) 1,3-butylene glycol 3.0 (3) Polyoxyethylene (25EO) oleyl ether 0.2 (4) Ethanol 10.0 (5) Methyl paraoxybenzoate 0.1 (6) Purified water Amount based on the total amount of 100 (7) Sperm beetle extract 2, Kacon extract-encapsulating liposomes 5.0 Preparation method: (5) to (4) Dissolve in, add to (6) together with (1) to (3), mix evenly, add (7) to this, and disperse. In addition, the (7) Koukai extract 2, Kakon extract-encapsulating liposomes were 50% by volume containing 2 1.0 (w / v)% of Koukon extract and 2.0 (w / v)% of Kakkon extract. 80 g of soybean lecithin was added to 100 mL of an aqueous ethanol solution, suspended at 55 ° C., and then sonicated to prepare liposomes, which were recovered by centrifugation.

[Example 23] Water-in-oil emollient cream (1) Liquid paraffin 30.0 (% by weight) (2) Microcrystalline wax 2.0 (3) Vaseline 5.0 (4) Diglyceryl dioleic acid Ester 5.0 (5) L-Glutamate sodium 1.6 (6) L-serine 0.4 (7) Propylene glycol 3.0 (8) Methyl paraoxybenzoate 0.1 (9) Sperm extract 10. 1 (10) bittern 1.5 (11) Purified water Amount based on 100 (12) Perfume 0.1 Manufacturing method: (5) and (6) are dissolved in a part of (11) to 50 ° C., Gradually add to (4) previously heated to 50 ° C. with stirring. These are mixed in advance and uniformly dispersed in (1) to (3) which are dissolved by heating at 70 ° C. Add (7) to (10) to (1
Add to the rest of 1), heat to 70 ° C, add with stirring, and emulsify with a homomixer. After cooling, (12) is added and mixed at 40 ° C.

[Example 24] Makeup base cream (1) Stearic acid 12.00 (% by weight) (2) Cetanol 2.00 (3) Glyceryl tri-2-ethylhexanoate 2.50 (4) Self-emulsification Type Glyceryl monostearate 2.00 (5) Propylene glycol 10.00 (6) Sperm extract 2 0.02 (7) Methyl paraoxybenzoate 0.10 (8) Lactic acid bacterium extract 0.05 (9) Water Potassium oxide 0.30 (10) Purified water Total amount 100 (11) Titanium oxide 2.00 (12) Red iron oxide 0.40 (13) Yellow iron oxide 0.10 (14) Perfume 0.10 Manufacturing method: ( The oil phase components of 1) to (4) are mixed and melted to 75 ° C. On the other hand, the components (5) to (10) are mixed, heated and dissolved, and the pigment components (11) to (13) are added thereto, and the components are uniformly dispersed with a homomixer to 75 ° C. Then, the oil phase component is added to the aqueous phase component to uniformly emulsify with a homomixer,
After cooling, (14) is added and mixed at 40 ° C.

Example 25 Milky liquid foundation (1) Stearic acid 2.00 (% by weight) (2) Squalane 5.00 (3) Octyldodecyl myristate 5.00 (4) Cetanol 1.00 (5) Decaglyceryl monoisopalmitic acid ester 9.00 (6) 1,3-butyleneglycol 6.00 (7) Methyl paraoxybenzoate 0.10 (8) Potassium hydroxide 0.08 (9) Purified water Total 100 Amount (10) Titanium oxide 9.00 (11) Talc 7.40 (12) Red iron oxide 0.50 (13) Yellow iron oxide 1.10 (14) Black iron oxide 0.10 (15) Sperm extract 1 0.02 (16) Kacon extract 0.05 (17) Perfume 0.15 Production method: The oil phase components (1) to (5) are mixed and dissolved to 75 ° C. On the other hand, the water phase components (6) to (9) are mixed, heated and dissolved, and the pigment components (10) to (14) are added thereto, and the mixture is uniformly dispersed with a homomixer to 75 ° C. Next, the oil phase component is added to the aqueous phase component, and the mixture is uniformly emulsified with a homomixer. After cooling, (15) to (17) are added and mixed at 40 ° C.

[Example 26] Hand cream (1) Cetanol 4.00 (% by weight) (2) Vaseline 2.00 (3) Liquid paraffin 10.00 (4) Glyceryl monostearate 1.50 (5) Polyoxyethylene (60 E.O.) glyceryl isostearic acid ester 2.50 (6) Tocopherol acetate 0.25 (7) Glycerin 20.00 (8) Methyl paraoxybenzoate 0.10 (9) Sperm extract 10. 05 (10) DNA-K 0.02 (11) Purified water Amount with 100 as the total amount Production method: Mix and dissolve the oil phase components (1) to (6) to 75 ° C. On the other hand, (8) and (9) are dissolved in (7) and (10) together with (11)
In addition, it mixes and melt | dissolves and makes it 75 degreeC. Next, the oil phase component is added to the aqueous phase component, and the mixture is uniformly emulsified with a homomixer and cooled.

Example 27 Pack (1) Purified Water Amount (% by Weight) Based on Total Amount (2) Polyvinyl Alcohol 12.5 (3) Ethanol 10.0 (4) Glycerin 5.0 (5) Polyethylene Glycol (average molecular weight 1540) 3.0 (6) Scallion extract 2 0.3 (7) Yeast culture supernatant 0.2 Production method: The components (2) to (7) are sequentially added to (1),
Mix, dissolve and homogenize.

[0130]   Example 28 Hair artic (1) Ethanol 50.0 (wt%) (2) Amount of purified water as 100 (3) Sodium citrate 0.1 (4) Scallop extract 1 0.2 Manufacturing method: Components (1) to (4) are mixed and uniformly dissolved.

Example 29 Hair Shampoo (1) Sodium Alkyl Ether Sulfate 18.0 (% by Weight) (2) Coconut Oil Fatty Acid Diethanolamide 2.0 (3) Propylene Glycol 2.0 (4) Sperm Extract 2 0.5 (5) Yeast extract 0.1 (6) Purified water Amount with 100 as the total amount Production method: The components (1) to (5) are sequentially added to (6),
Make it uniform.

[Example 30] Hair rinse (1) Cetanol 3.0 (wt%) (2) Stearyl trimethyl ammonium chloride 0.7 (3) Glycerin 3.0 (4) N-cocoyl-L-arginine ethyl ester -DL-pyrrolidone carboxylate 0.1 (5) Bifidobacteria extract 0.1 (6) Sperm extract 1 0.1 (7) Purified water Amount with 100 as the total amount Production method: (1) to (6) Add the ingredients in step (7) to
Mix.

Example 31 Liquid Body Shampoo (1) N-lauroyl-L-glutamic acid triethanolamine (30.0% by weight aqueous solution) 20.0 (% by weight) (2) N-lauroylmethyl taurine sodium (30) 0.0 wt% aqueous solution) 10.0 (3) triethanolamine laurate 10.0 (4) triethanolamine myristate 10.0 (5) lauroyl imidazolinium betaine 5.0 (6) lauroyl diethanolamide 5. 0 (7) Propylene glycol 7.0 (8) Coleoptera extract 2 0.5 (9) Chamomile extract 0.5 (10) Purified water Amount with 100 as the total amount Process: (1) to (9) components Are sequentially added to (10) and mixed.

Example 32 Face Wash Foam (1) Myristic Acid 18.0 (wt%) (2) Palmitic Acid 3.0 (3) Stearic Acid 7.0 (4) Mixed Fatty Acid Triglyceride 0.1 (5) Stearyl glycyrrhetinate 0.1 (6) Self-emulsifying glyceryl monostearate 3.0 (7) Purified water Amount based on 100 (8) Glycerin 17.0 (9) Potassium hydroxide 7.8 (10) Di Glycerin 3.0 (11) 1,3-butylene glycol 1.0 (12) N-stearolyl-L-glutamate disodium 1.0 (13) Methyl paraoxybenzoate 0.1 (14) Sperm extract 10. 2 (15) Kidachi aloe extract 0.6 Manufacturing method: The oil phase components of (1) to (6) are mixed and dissolved by heating to 70 ° C. The aqueous phase components (7) to (15) are mixed, dissolved and heated to 70 ° C. The above oil phase is gradually added to this aqueous phase component for saponification, and then cooled while mixing.

For Examples 1 to 32, 2
No change in condition was observed when stored at 5 ° C for 6 months, and no problem skin irritation reaction was observed in a 48-hour back occlusion patch test conducted by 30 male panelists.

[0136]

As described in detail above, according to the present invention, it is possible to obtain a skin external preparation having an excellent moisturizing effect and synergistically improving the skin aging symptoms such as wrinkles and the rough skin improving effect. did it.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61K 7/075 A61K 7/075 7/08 7/08 7/48 7/48 7/50 7/50 F Term (reference) 4C083 AA031 AA032 AA071 AA111 AA112 AA122 AB032 AB232 AB242 AB342 AB442 AC012 AC022 AC072 AC102 AC122 AC182 AC242 AC301 AC352 AC422 AC432 AC442 AC482 AC582 AC641 AC642 AC662 AC692 AD621 AD532 AD112 AD152 AD112 AD152 AD152 AD112 AD152 AD152 AD152 AD152 AD112 AD152 AD152 AD112 AD152 AD152 AD112 AD152 AD152 AD112 AD152 AD152 AD112 AD152 AD152 AD112 AD152 AD152 AD112 AD662 BB51 CC03 CC04 CC05 CC07 CC11 CC12 CC23 CC38 CC39 DD23 DD27 DD31 DD41 EE06 EE12 EE28

Claims (2)

[Claims] 1. A skin external preparation comprising a Kou-shiki extract and a cell activating agent. 2. A cell activator is ceramides and ceramide-like structure materials, yeast extract and yeast culture supernatant, pueraria root, Cordyceps sinensis, Ganoderma, the herbal extract of Amanin, asparagus (Asparagus) Genus, avocado ( Per
sea americana Mill.), aloe (Aloe) plants of the genus, apricot (Prunus armeniaca L. var. ansu Maxim .), ginkgo (Ginkgo biloba L.), Fagus japonica (Fagus japonica Max
im.), garlic ( Allium sativum L. f. pekinens
e Makino), Panax ginseng CAMeye
r), chamomile (Matricaria chamomilla L.), cucumber (Cucumis sativus L.), Calendula (Calendula ar
vensis L.), ghetto ( Alpinia speciosa (Wendl.)
K. Schum.), Shiitake mushroom ( Lentinus edodes Sing.),
Cinnamon (Kiwi) ( Actinidia chinensis Planc
h.), horsetail ( Equisetum arvense L.), garlic ( Allium sativum L.), yarrow ( A )
chillea aplina L .; Achillea sibilica Ledeb.), Senburi ( Swertiajaponica Makino), Stephania cepharantha Hayata, Lactuca sativa L., Clintonia
udensis Trautv. et Mey .; Clintonia borealis ),
Capsicum annuum L., Houttu
ynia cordata Thunb.), Horse chestnut ( Aesculus turbinat )
a Blume), grape ( Vitis vinifera L.), beech ( Fagu )
s crenata Blume), loofah ( Luffa cylindrica M.Roem
en), safflower ( Carthamus tinctorius L.), mannen wax ( Rosmarinus officinalis L.), mandarin orange ( Citru )
s ) genus plant, Sapindus mukurossi Gaert
n.), purple ( Lithospermum officinale L. var. er
ythrorhizon Maxim.), Salixae plants, Eucalyptus plants, Lilium plants, Eriophorum L. plant extracts, hydroxy fatty acids and their salts and derivatives, nucleic acids and their related substances, proteins extracted from the eggshell membrane, sericin and its hydrolysis product, lactobacillus extract, bifidobacteria extract, algae extract, bittern components, vitamin A, vitamin B _2, vitamin B ₆ such, nicotinic acid And salts and derivatives thereof, vitamin Ds such as ergocalciferol and cholecalciferol, vitamin Hs, spleen extract of mammals such as human and bovine, basic and acidic fibroblast growth factors, epidermal growth factor, The external preparation for skin according to claim 1, which is one or more selected from the following.