JP2002526756A - 生細胞におけるタンパク質−タンパク質相互作用の測定方法 - Google Patents

生細胞におけるタンパク質−タンパク質相互作用の測定方法

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Publication number
JP2002526756A
JP2002526756A JP2000574129A JP2000574129A JP2002526756A JP 2002526756 A JP2002526756 A JP 2002526756A JP 2000574129 A JP2000574129 A JP 2000574129A JP 2000574129 A JP2000574129 A JP 2000574129A JP 2002526756 A JP2002526756 A JP 2002526756A
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JP
Japan
Prior art keywords
protein
nucleic acid
interest
cell
library
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
JP2000574129A
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English (en)
Japanese (ja)
Inventor
メイヤー，トバイアス
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Duke University
Original Assignee
Duke University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Duke University filed Critical Duke University
Publication of JP2002526756A publication Critical patent/JP2002526756A/ja
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2000574129A 1998-09-24 1999-08-24 生細胞におけるタンパク質−タンパク質相互作用の測定方法 Withdrawn JP2002526756A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10163798P 1998-09-24 1998-09-24
US60/101,637 1998-09-24
PCT/US1999/019118 WO2000017221A1 (fr) 1998-09-24 1999-08-24 Procede de mesure des interactions proteine-proteine dans des cellules vivantes

Publications (1)

Publication Number Publication Date
JP2002526756A true JP2002526756A (ja) 2002-08-20

Family

ID=22285675

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2000574129A Withdrawn JP2002526756A (ja) 1998-09-24 1999-08-24 生細胞におけるタンパク質−タンパク質相互作用の測定方法

Country Status (5)

Country Link
EP (1) EP1115734A4 (fr)
JP (1) JP2002526756A (fr)
AU (1) AU5578999A (fr)
CA (1) CA2345392A1 (fr)
WO (1) WO2000017221A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010115192A (ja) * 2008-11-12 2010-05-27 Korea Basic Science Inst 生体高分子物質の相互作用検出方法

Families Citing this family (18)

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WO2001000866A1 (fr) * 1999-06-26 2001-01-04 Odyssey Pharmaceuticals, Inc. Selection in vivo bibliotheque contre bibliotheque d'interactions proteine-proteine optimisees
JP2004502434A (ja) 2000-07-04 2004-01-29 バイオイメージ　エイ／エス 細胞成分間の相互作用に関する量的情報の抽出方法
AU2002362537A1 (en) * 2001-10-01 2003-04-14 Bioimage A/S A method of detecting intracellular protein-protein interactions using three heterologous conjugates
US20050214734A1 (en) * 2002-04-01 2005-09-29 Seishi Kato Cell population provided with identification codes and method of screening cell population
US7771952B2 (en) 2002-06-26 2010-08-10 Abott Laboratories Modulators and modulation of the interaction between RGM and Neogenin
JP2004085528A (ja) * 2002-07-05 2004-03-18 Kansai Tlo Kk 拡散定数変化による蛋白質の会合検出方法及び装置
EP1533619B1 (fr) 2003-11-20 2009-04-29 F. Hoffmann-Roche AG Marqueurs spécifiques du syndrome métabolique.
EP1928905B1 (fr) 2005-09-30 2015-04-15 AbbVie Deutschland GmbH & Co KG Domaines de liaison de proteines de la famille proteinique des molecules de guidage repulsif (rgm), fragments fonctionnels de ces domaines et leur utilisation
US8962803B2 (en) 2008-02-29 2015-02-24 AbbVie Deutschland GmbH & Co. KG Antibodies against the RGM A protein and uses thereof
MX2012006560A (es) 2009-12-08 2012-10-05 Abbott Gmbh & Co Kg Anticuerpos monoclonales contra la proteina rgm a para utilizarse en el tratamiento de degeneracion de capa de fibra de nervio retinal.
WO2011083147A1 (fr) 2010-01-08 2011-07-14 Cemm-Forschungsinstitut Für Molekulare Medizin Gmbh Inhibition de wave1 dans l'intervention médicale de maladies inflammatoires et/ou d'infections causées par un pathogène
US20130116304A1 (en) 2010-04-19 2013-05-09 Ernst R. Werner Tmem195 encodes for tetrahydrobiopterin-dependent alkylglycerol monooxygenase activity
US9494595B2 (en) 2011-12-05 2016-11-15 Medical & Biological Laboratories Co., Ltd. Method for detecting protein-protein interaction
IL297229A (en) 2012-01-27 2022-12-01 Abbvie Inc The composition and method for the diagnosis and treatment of diseases related to the degeneration of nerve cells
EP2823044B1 (fr) 2012-03-06 2017-12-06 VIB vzw Protéine de fusion span-kinase de membrane et utilisations associées
JPWO2015190529A1 (ja) 2014-06-10 2017-04-20 株式会社医学生物学研究所 タンパク質間相互作用の判定方法
US10591486B2 (en) 2014-07-23 2020-03-17 Institut National De La Sante Et De La Recherche Medicale (Inserm) Methods and tools for detecting interactions in eukaryotic cells using microtubule structures and dynamics
KR101823990B1 (ko) * 2016-09-07 2018-01-31 포항공과대학교 산학협력단 살아있는 세포에서 막 단백질 간의 상호작용을 정량적으로 측정하는 방법

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5804604A (en) * 1989-12-21 1998-09-08 Biogen, Inc. Tat-derived transport polypeptides and fusion proteins
US5427908A (en) * 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
US5871907A (en) * 1991-05-15 1999-02-16 Medical Research Council Methods for producing members of specific binding pairs
JPH08504091A (ja) * 1992-09-22 1996-05-07 メディカル・リサーチ・カウンシル 外部表面に非ウィルス性ポリペプチドを提示する組換えウィルス
AU4483096A (en) * 1995-01-31 1996-08-21 Novo Nordisk A/S A method of detecting biologically active substances
AU3801997A (en) * 1996-07-16 1998-02-09 Regents Of The University Of California, The Assays for protein kinases using fluorescent protein substrates
CA2196496A1 (fr) * 1997-01-31 1998-07-31 Stephen William Watson Michnick Epreuve de complementation de fragments de proteines pour la detection d'interactions entre proteines

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010115192A (ja) * 2008-11-12 2010-05-27 Korea Basic Science Inst 生体高分子物質の相互作用検出方法

Also Published As

Publication number Publication date
WO2000017221A9 (fr) 2000-06-29
CA2345392A1 (fr) 2000-03-30
EP1115734A4 (fr) 2004-07-21
EP1115734A1 (fr) 2001-07-18
WO2000017221A1 (fr) 2000-03-30
AU5578999A (en) 2000-04-10

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Legal Events

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A300 Withdrawal of application because of no request for examination

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Effective date: 20061107