EP1115734A4 - Procede de mesure des interactions proteine-proteine dans des cellules vivantes - Google Patents
Procede de mesure des interactions proteine-proteine dans des cellules vivantesInfo
- Publication number
- EP1115734A4 EP1115734A4 EP99942401A EP99942401A EP1115734A4 EP 1115734 A4 EP1115734 A4 EP 1115734A4 EP 99942401 A EP99942401 A EP 99942401A EP 99942401 A EP99942401 A EP 99942401A EP 1115734 A4 EP1115734 A4 EP 1115734A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- protein
- living cells
- measuring
- interactions
- protein interactions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10163798P | 1998-09-24 | 1998-09-24 | |
US101637P | 1998-09-24 | ||
PCT/US1999/019118 WO2000017221A1 (fr) | 1998-09-24 | 1999-08-24 | Procede de mesure des interactions proteine-proteine dans des cellules vivantes |
Publications (2)
Publication Number | Publication Date |
---|---|
EP1115734A1 EP1115734A1 (fr) | 2001-07-18 |
EP1115734A4 true EP1115734A4 (fr) | 2004-07-21 |
Family
ID=22285675
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP99942401A Withdrawn EP1115734A4 (fr) | 1998-09-24 | 1999-08-24 | Procede de mesure des interactions proteine-proteine dans des cellules vivantes |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1115734A4 (fr) |
JP (1) | JP2002526756A (fr) |
AU (1) | AU5578999A (fr) |
CA (1) | CA2345392A1 (fr) |
WO (1) | WO2000017221A1 (fr) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2003503067A (ja) * | 1999-06-26 | 2003-01-28 | オデュッセイ・ファーマシューティカルズ,インコーポレイテッド | 最適化された蛋白質−蛋白質相互作用のインビボライブラリー対ライブラリー選択 |
US7282347B2 (en) | 2000-07-04 | 2007-10-16 | Fisher Bioimage Aps | Method for extracting quantitative information relating to interactions between cellular components |
AU2002362537A1 (en) * | 2001-10-01 | 2003-04-14 | Bioimage A/S | A method of detecting intracellular protein-protein interactions using three heterologous conjugates |
WO2003083112A1 (fr) * | 2002-04-01 | 2003-10-09 | Japan Science And Technology Agency | Population de cellules pourvues de codes d'identification et procedes pour le criblage de population de cellules |
US7771952B2 (en) | 2002-06-26 | 2010-08-10 | Abott Laboratories | Modulators and modulation of the interaction between RGM and Neogenin |
JP2004085528A (ja) * | 2002-07-05 | 2004-03-18 | Kansai Tlo Kk | 拡散定数変化による蛋白質の会合検出方法及び装置 |
EP1533619B1 (fr) | 2003-11-20 | 2009-04-29 | F. Hoffmann-Roche AG | Marqueurs spécifiques du syndrome métabolique. |
JP2009510002A (ja) | 2005-09-30 | 2009-03-12 | アボット ゲゼルシャフト ミット ベシュレンクテル ハフツング ウント コンパニー コマンディトゲゼルシャフト | 反発誘導分子(rgm)タンパク質ファミリーのタンパク質の結合ドメイン、及びその機能的断片、及びそれらの使用 |
US8962803B2 (en) | 2008-02-29 | 2015-02-24 | AbbVie Deutschland GmbH & Co. KG | Antibodies against the RGM A protein and uses thereof |
KR100948767B1 (ko) * | 2008-11-12 | 2010-03-23 | 한국기초과학지원연구원 | 생체 고분자 물질의 상호작용 검출 방법 |
CA2780069C (fr) | 2009-12-08 | 2018-07-17 | Abbott Gmbh & Co. Kg | Anticorps monoclonaux contre la proteine rgm a destines a etre utilises dans le traitement de la degenerescence de la couche de fibres nerveuses retiniennes |
WO2011083147A1 (fr) | 2010-01-08 | 2011-07-14 | Cemm-Forschungsinstitut Für Molekulare Medizin Gmbh | Inhibition de wave1 dans l'intervention médicale de maladies inflammatoires et/ou d'infections causées par un pathogène |
WO2011131626A1 (fr) | 2010-04-19 | 2011-10-27 | Medizinische Universität Innsbruck | Tmem195 code pour l'activité alkylglycérol monooxygénase dépendante de la tétrahydrobioptérine |
US9494595B2 (en) | 2011-12-05 | 2016-11-15 | Medical & Biological Laboratories Co., Ltd. | Method for detecting protein-protein interaction |
MY194587A (en) | 2012-01-27 | 2022-12-05 | Abbvie Inc | Composition and method for the diagnosis and treatment of diseases associated with neurite degeneration |
CA2866097C (fr) | 2012-03-06 | 2021-02-02 | Vib Vzw | Proteine de fusion transmembranaire-kinase et les utilisations de celle-ci |
SG11201610359QA (en) | 2014-06-10 | 2017-01-27 | Med & Biological Lab Co Ltd | Method for determining a protein-protein interaction |
EP3172563B1 (fr) | 2014-07-23 | 2019-06-12 | Institut National de la Sante et de la Recherche Medicale (INSERM) | Procédés pour détecter des interactions dans des cellules eucaryotes au moyen de structures de microtubules et dynamiques |
KR101823990B1 (ko) * | 2016-09-07 | 2018-01-31 | 포항공과대학교 산학협력단 | 살아있는 세포에서 막 단백질 간의 상호작용을 정량적으로 측정하는 방법 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1996023898A1 (fr) * | 1995-01-31 | 1996-08-08 | Novo Nordisk A/S | Procede de detection de substances biologiquement actives |
WO1998002571A1 (fr) * | 1996-07-16 | 1998-01-22 | The Regents Of The University Of California | Dosages de proteines kinases faisant appel a des substrats proteiques fluorescents |
WO1998034120A1 (fr) * | 1997-01-31 | 1998-08-06 | Universite De Montreal | Analyses par complementation de fragments proteiques pour detecter des interactions biomoleculaires |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5804604A (en) * | 1989-12-21 | 1998-09-08 | Biogen, Inc. | Tat-derived transport polypeptides and fusion proteins |
US5427908A (en) * | 1990-05-01 | 1995-06-27 | Affymax Technologies N.V. | Recombinant library screening methods |
DE69230142T2 (de) * | 1991-05-15 | 2000-03-09 | Cambridge Antibody Technology Ltd. | Verfahren zur herstellung von spezifischen bindungspaargliedern |
ATE245703T1 (de) * | 1992-09-22 | 2003-08-15 | Biofocus Discovery Ltd | Rekombinante viren, die an ihrer äusseren oberfläche ein nichtvirales polypeptid präsentieren |
-
1999
- 1999-08-24 AU AU55789/99A patent/AU5578999A/en not_active Abandoned
- 1999-08-24 EP EP99942401A patent/EP1115734A4/fr not_active Withdrawn
- 1999-08-24 CA CA002345392A patent/CA2345392A1/fr not_active Abandoned
- 1999-08-24 WO PCT/US1999/019118 patent/WO2000017221A1/fr not_active Application Discontinuation
- 1999-08-24 JP JP2000574129A patent/JP2002526756A/ja not_active Withdrawn
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1996023898A1 (fr) * | 1995-01-31 | 1996-08-08 | Novo Nordisk A/S | Procede de detection de substances biologiquement actives |
WO1998002571A1 (fr) * | 1996-07-16 | 1998-01-22 | The Regents Of The University Of California | Dosages de proteines kinases faisant appel a des substrats proteiques fluorescents |
WO1998034120A1 (fr) * | 1997-01-31 | 1998-08-06 | Universite De Montreal | Analyses par complementation de fragments proteiques pour detecter des interactions biomoleculaires |
Non-Patent Citations (4)
Title |
---|
BRODER Y C ET AL: "The ras recruitment system, a novel approach to the study of protein-protein interactions", CURRENT BIOLOGY, CURRENT SCIENCE,, GB, vol. 8, no. 20, 8 October 1998 (1998-10-08), pages 1121 - 1124, XP002135902, ISSN: 0960-9822 * |
MAROUN M ET AL: "A NOVEL IN VIVO ASSAY FOR THE ANALYSIS OF PROTEIN-PROTEIN INTERACTION", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 27, no. 13, 1999, pages COMPLETE, XP000904543, ISSN: 0305-1048 * |
SAKAI ET AL: "Translocation of protein kinase C-gamma and epsilon - Direct visualization in living cells using fusion protein with green fluorescent protein", THE JOURNAL OF CELL BIOLOGY, ROCKEFELLER UNIVERSITY PRESS, US, 1997, XP002078902, ISSN: 0021-9525 * |
See also references of WO0017221A1 * |
Also Published As
Publication number | Publication date |
---|---|
WO2000017221A1 (fr) | 2000-03-30 |
CA2345392A1 (fr) | 2000-03-30 |
AU5578999A (en) | 2000-04-10 |
WO2000017221A9 (fr) | 2000-06-29 |
JP2002526756A (ja) | 2002-08-20 |
EP1115734A1 (fr) | 2001-07-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP1115734A4 (fr) | Procede de mesure des interactions proteine-proteine dans des cellules vivantes | |
IL142567A0 (en) | In situ method of analyzing cells | |
EP1033556A4 (fr) | Procede de mesure tridimensionnelle et instrument d'etude l'utilisant | |
GB9929381D0 (en) | A method of assessing the amount of nucleic acid in a sample | |
EP1212073A4 (fr) | Procede de mesure de l'activite du facteur coagulant | |
AU2142400A (en) | Novel ion-conducting materials suitable for use in electrochemical applications and methods related thereto | |
IL140851A0 (en) | In situ method of analyzing cells | |
NO20004856L (no) | Gelelektrolytt og gelelektrolyttcelle | |
EP1120846A4 (fr) | Cellule et procede d'inspection de cellule | |
AU5912099A (en) | System for measuring tissue size and marbling in an animal | |
GB2305247B (en) | Electrochemical measuring cell | |
EP1109931A4 (fr) | PROCEDE D'ETUDE D'INTERACTIONS DE PROTEINES $i(IN VIVO) | |
DE59610015D1 (de) | Elektrochemische Messzelle | |
NO20005635D0 (no) | Fremgangsmåte for fremstilling av elektrode og gelelektrolyttcelle | |
AU5070200A (en) | Method and device for measuring the degree of organization of water in human andanimal bodies | |
AU1409700A (en) | Method for counting living cells | |
GB2343518B (en) | Method of operating an electrochemical measurement cell | |
GB9413525D0 (en) | Improvements in or relating to electrochemical instruments | |
GB2299863B (en) | Electrochemical measuring cell | |
AU2869599A (en) | Cell analysis methods and apparatus | |
GB9900118D0 (en) | A method for determining the temperature of a single cell in a cell sample or tissue biopsy and methods of use thereof | |
AU5901501A (en) | Measurement of nutrient uptake in cells and methods based thereon | |
EP1151289A4 (fr) | Detecteur electrochimique de gaz et procede de detection de gaz | |
PL345518A1 (en) | Detection of apoptotic cells in living nematodes | |
EP1210451A4 (fr) | Methode rapide destinee a mesurer la concentration de glucides complexes dans le tissu mammalien |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 20010424 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE |
|
A4 | Supplementary search report drawn up and despatched |
Effective date: 20040609 |
|
RIC1 | Information provided on ipc code assigned before grant |
Ipc: 7G 01N 33/68 B Ipc: 7G 01N 33/50 B Ipc: 7C 12Q 1/68 B Ipc: 7C 07H 21/04 A |
|
17Q | First examination report despatched |
Effective date: 20050527 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 20051007 |