EP1115734A4 - Method of measuring protein-protein interactions in living cells - Google Patents

Method of measuring protein-protein interactions in living cells

Info

Publication number
EP1115734A4
EP1115734A4 EP99942401A EP99942401A EP1115734A4 EP 1115734 A4 EP1115734 A4 EP 1115734A4 EP 99942401 A EP99942401 A EP 99942401A EP 99942401 A EP99942401 A EP 99942401A EP 1115734 A4 EP1115734 A4 EP 1115734A4
Authority
EP
European Patent Office
Prior art keywords
protein
living cells
measuring
interactions
protein interactions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP99942401A
Other languages
German (de)
French (fr)
Other versions
EP1115734A1 (en
Inventor
Tobias Meyer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Duke University
Original Assignee
Duke University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Duke University filed Critical Duke University
Publication of EP1115734A1 publication Critical patent/EP1115734A1/en
Publication of EP1115734A4 publication Critical patent/EP1115734A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
EP99942401A 1998-09-24 1999-08-24 Method of measuring protein-protein interactions in living cells Withdrawn EP1115734A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10163798P 1998-09-24 1998-09-24
US101637P 1998-09-24
PCT/US1999/019118 WO2000017221A1 (en) 1998-09-24 1999-08-24 Method of measuring protein-protein interactions in living cells

Publications (2)

Publication Number Publication Date
EP1115734A1 EP1115734A1 (en) 2001-07-18
EP1115734A4 true EP1115734A4 (en) 2004-07-21

Family

ID=22285675

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99942401A Withdrawn EP1115734A4 (en) 1998-09-24 1999-08-24 Method of measuring protein-protein interactions in living cells

Country Status (5)

Country Link
EP (1) EP1115734A4 (en)
JP (1) JP2002526756A (en)
AU (1) AU5578999A (en)
CA (1) CA2345392A1 (en)
WO (1) WO2000017221A1 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6054900A (en) * 1999-06-26 2001-01-31 Odyssey Pharmaceuticals, Inc. An in vivo library-versus-library selection of optimized protein-protein interactions
AU2001270481A1 (en) 2000-07-04 2002-01-14 Bioimage A/S A method for extracting quantitative information relating to interactions between cellular components
EP1440319A2 (en) * 2001-10-01 2004-07-28 BioImage A/S An improved method to detect interactions between cellular components in intact living cells, and to extract quantitative information relating to those interactions by fluorescence redistribution
CA2488233A1 (en) * 2002-04-01 2003-10-09 Japan Science And Technology Agency Cell population with identification codes and method of screening cell population
WO2004003150A2 (en) 2002-06-26 2004-01-08 Yale University Modulators and modulation of the interacton between rgm and neogenin
JP2004085528A (en) * 2002-07-05 2004-03-18 Kansai Tlo Kk Method and apparatus for detecting protein association by change in diffusion constant
DE602004020855D1 (en) 2003-11-20 2009-06-10 Hoffmann La Roche Specific markers for metabolic syndromes
ES2542501T3 (en) 2005-09-30 2015-08-06 Abbvie Deutschland Gmbh & Co Kg Protein binding domains of the protein family of repulsive targeting molecules (RGM) and functional fragments thereof, as well as their use
US8962803B2 (en) 2008-02-29 2015-02-24 AbbVie Deutschland GmbH & Co. KG Antibodies against the RGM A protein and uses thereof
KR100948767B1 (en) * 2008-11-12 2010-03-23 한국기초과학지원연구원 Method for detecting interactions between two and more biological macromolecules
US9175075B2 (en) 2009-12-08 2015-11-03 AbbVie Deutschland GmbH & Co. KG Methods of treating retinal nerve fiber layer degeneration with monoclonal antibodies against a retinal guidance molecule (RGM) protein
WO2011083147A1 (en) 2010-01-08 2011-07-14 Cemm-Forschungsinstitut Für Molekulare Medizin Gmbh Wave1 inhibition in the medical intervention of inflammatory diseases and/or infections caused by a pathogen
EP2561068A1 (en) 2010-04-19 2013-02-27 Medizinische Universität Innsbruck Tmem195 encodes for tetrahydrobiopterin-dependent alkylglycerol monooxygenase activity
KR102051159B1 (en) 2011-12-05 2019-12-02 가부시키가이샤 이가쿠세이부츠가쿠겐큐쇼 Method for detecting protein-protein interaction
AU2013211939C1 (en) 2012-01-27 2018-06-07 AbbVie Deutschland GmbH & Co. KG Composition and method for diagnosis and treatment of diseases associated with neurite degeneration
EP2823044B1 (en) * 2012-03-06 2017-12-06 VIB vzw Membrane span-kinase fusion protein and the uses thereof
SG10201810877TA (en) 2014-06-10 2019-01-30 Medical & Biological Laboratories Co Ltd Method for determining a protein-protein interaction
EP3172563B1 (en) 2014-07-23 2019-06-12 Institut National de la Sante et de la Recherche Medicale (INSERM) Methods for detecting interactions in eukaryotic cells using microtubule structures and dynamics
KR101823990B1 (en) * 2016-09-07 2018-01-31 포항공과대학교 산학협력단 A Method for Quantitative Analysis of Transient Interactions of Membrane Proteins in a Single Living Cell

Citations (3)

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Publication number Priority date Publication date Assignee Title
WO1996023898A1 (en) * 1995-01-31 1996-08-08 Novo Nordisk A/S A method of detecting biologically active substances
WO1998002571A1 (en) * 1996-07-16 1998-01-22 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
WO1998034120A1 (en) * 1997-01-31 1998-08-06 Universite De Montreal Protein fragment complementation assays to detect biomolecular interactions

Family Cites Families (4)

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Publication number Priority date Publication date Assignee Title
US5804604A (en) * 1989-12-21 1998-09-08 Biogen, Inc. Tat-derived transport polypeptides and fusion proteins
US5427908A (en) * 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
DE69230142T2 (en) * 1991-05-15 2000-03-09 Cambridge Antibody Tech METHOD FOR PRODUCING SPECIFIC BINDING PAIRS
JPH08504091A (en) * 1992-09-22 1996-05-07 メディカル・リサーチ・カウンシル Recombinant virus displaying non-viral polypeptides on the outer surface

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996023898A1 (en) * 1995-01-31 1996-08-08 Novo Nordisk A/S A method of detecting biologically active substances
WO1998002571A1 (en) * 1996-07-16 1998-01-22 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
WO1998034120A1 (en) * 1997-01-31 1998-08-06 Universite De Montreal Protein fragment complementation assays to detect biomolecular interactions

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BRODER Y C ET AL: "The ras recruitment system, a novel approach to the study of protein-protein interactions", CURRENT BIOLOGY, CURRENT SCIENCE,, GB, vol. 8, no. 20, 8 October 1998 (1998-10-08), pages 1121 - 1124, XP002135902, ISSN: 0960-9822 *
MAROUN M ET AL: "A NOVEL IN VIVO ASSAY FOR THE ANALYSIS OF PROTEIN-PROTEIN INTERACTION", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 27, no. 13, 1999, pages COMPLETE, XP000904543, ISSN: 0305-1048 *
SAKAI ET AL: "Translocation of protein kinase C-gamma and epsilon - Direct visualization in living cells using fusion protein with green fluorescent protein", THE JOURNAL OF CELL BIOLOGY, ROCKEFELLER UNIVERSITY PRESS, US, 1997, XP002078902, ISSN: 0021-9525 *
See also references of WO0017221A1 *

Also Published As

Publication number Publication date
AU5578999A (en) 2000-04-10
WO2000017221A1 (en) 2000-03-30
CA2345392A1 (en) 2000-03-30
JP2002526756A (en) 2002-08-20
EP1115734A1 (en) 2001-07-18
WO2000017221A9 (en) 2000-06-29

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Legal Events

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