JP2002525605A - サルモネラ抗原処方物及びサルモネラ抗体検出用キット - Google Patents
サルモネラ抗原処方物及びサルモネラ抗体検出用キットInfo
- Publication number
- JP2002525605A JP2002525605A JP2000571263A JP2000571263A JP2002525605A JP 2002525605 A JP2002525605 A JP 2002525605A JP 2000571263 A JP2000571263 A JP 2000571263A JP 2000571263 A JP2000571263 A JP 2000571263A JP 2002525605 A JP2002525605 A JP 2002525605A
- Authority
- JP
- Japan
- Prior art keywords
- salmonella
- lipopolysaccharide
- antigen
- complex
- serum albumin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 241000607142 Salmonella Species 0.000 title claims abstract description 104
- 239000000427 antigen Substances 0.000 title claims abstract description 84
- 102000036639 antigens Human genes 0.000 title claims abstract description 74
- 108091007433 antigens Proteins 0.000 title claims abstract description 74
- 239000000203 mixture Substances 0.000 title claims abstract description 55
- 238000009472 formulation Methods 0.000 title claims abstract description 30
- 239000002158 endotoxin Substances 0.000 claims abstract description 32
- 229920006008 lipopolysaccharide Polymers 0.000 claims abstract description 32
- 210000002966 serum Anatomy 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 20
- 241001138501 Salmonella enterica Species 0.000 claims abstract description 14
- 230000001900 immune effect Effects 0.000 claims abstract description 6
- 210000002969 egg yolk Anatomy 0.000 claims abstract description 5
- 238000002965 ELISA Methods 0.000 claims abstract description 3
- 239000000872 buffer Substances 0.000 claims description 21
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 claims description 18
- 238000001514 detection method Methods 0.000 claims description 16
- 239000005018 casein Substances 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 14
- 238000002360 preparation method Methods 0.000 claims description 13
- 238000005406 washing Methods 0.000 claims description 10
- 108010088751 Albumins Proteins 0.000 claims description 9
- 102000009027 Albumins Human genes 0.000 claims description 9
- 239000000758 substrate Substances 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 239000013024 dilution buffer Substances 0.000 claims description 8
- 229940098773 bovine serum albumin Drugs 0.000 claims description 7
- 210000004027 cell Anatomy 0.000 claims description 7
- 239000012089 stop solution Substances 0.000 claims description 6
- 241001465754 Metazoa Species 0.000 claims description 5
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- 241000287828 Gallus gallus Species 0.000 claims description 4
- 235000021240 caseins Nutrition 0.000 claims description 4
- 235000013882 gravy Nutrition 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 2
- 102000013415 peroxidase activity proteins Human genes 0.000 claims description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 2
- 230000000890 antigenic effect Effects 0.000 claims 2
- 230000036046 immunoreaction Effects 0.000 claims 1
- 239000007787 solid Substances 0.000 abstract description 11
- 102000004169 proteins and genes Human genes 0.000 abstract description 9
- 108090000623 proteins and genes Proteins 0.000 abstract description 9
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 7
- 102000002322 Egg Proteins Human genes 0.000 abstract description 3
- 108010000912 Egg Proteins Proteins 0.000 abstract description 3
- 235000013345 egg yolk Nutrition 0.000 abstract description 3
- 238000005259 measurement Methods 0.000 abstract description 3
- 238000003018 immunoassay Methods 0.000 abstract description 2
- 230000000405 serological effect Effects 0.000 abstract description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 235000013372 meat Nutrition 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 30
- 238000012360 testing method Methods 0.000 description 23
- 238000005497 microtitration Methods 0.000 description 16
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 238000011002 quantification Methods 0.000 description 9
- 238000000576 coating method Methods 0.000 description 8
- 235000018102 proteins Nutrition 0.000 description 8
- 239000011534 wash buffer Substances 0.000 description 8
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 7
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 7
- 239000011248 coating agent Substances 0.000 description 7
- 239000012153 distilled water Substances 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 5
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 5
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 5
- 239000006285 cell suspension Substances 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 239000013641 positive control Substances 0.000 description 5
- 238000010079 rubber tapping Methods 0.000 description 5
- 241000282887 Suidae Species 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 239000013642 negative control Substances 0.000 description 4
- 229940124272 protein stabilizer Drugs 0.000 description 4
- 239000012898 sample dilution Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 241001354013 Salmonella enterica subsp. enterica serovar Enteritidis Species 0.000 description 3
- 241000282898 Sus scrofa Species 0.000 description 3
- 238000005273 aeration Methods 0.000 description 3
- 239000012141 concentrate Substances 0.000 description 3
- 230000009260 cross reactivity Effects 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- YRNWIFYIFSBPAU-UHFFFAOYSA-N 4-[4-(dimethylamino)phenyl]-n,n-dimethylaniline Chemical compound C1=CC(N(C)C)=CC=C1C1=CC=C(N(C)C)C=C1 YRNWIFYIFSBPAU-UHFFFAOYSA-N 0.000 description 2
- 241000271566 Aves Species 0.000 description 2
- 206010008631 Cholera Diseases 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 108010071390 Serum Albumin Proteins 0.000 description 2
- 102000007562 Serum Albumin Human genes 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000002405 diagnostic procedure Methods 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 239000008055 phosphate buffer solution Substances 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 2
- 238000002255 vaccination Methods 0.000 description 2
- 240000003291 Armoracia rusticana Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 206010039438 Salmonella Infections Diseases 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 125000005587 carbonate group Chemical group 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000008098 formaldehyde solution Substances 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 229940127121 immunoconjugate Drugs 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 206010039447 salmonellosis Diseases 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 229940033663 thimerosal Drugs 0.000 description 1
- 229960004906 thiomersal Drugs 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19842609.7 | 1998-09-17 | ||
| DE1998142609 DE19842609C2 (de) | 1998-09-17 | 1998-09-17 | Salmonellenantigengemisch und Kit zur Bestimmung von Antikörpern gegen Salmonellen |
| PCT/DE1999/003017 WO2000017653A2 (fr) | 1998-09-17 | 1999-09-17 | Composition d'antigenes contre les salmonelles et kit permettant de detecter des anticorps contre des salmonelles |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2002525605A true JP2002525605A (ja) | 2002-08-13 |
Family
ID=7881286
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000571263A Withdrawn JP2002525605A (ja) | 1998-09-17 | 1999-09-17 | サルモネラ抗原処方物及びサルモネラ抗体検出用キット |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP1114321A2 (fr) |
| JP (1) | JP2002525605A (fr) |
| CN (1) | CN1153971C (fr) |
| BR (1) | BR9913837A (fr) |
| DE (1) | DE19842609C2 (fr) |
| WO (1) | WO2000017653A2 (fr) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014503829A (ja) * | 2011-01-28 | 2014-02-13 | イミュノサイエンシス ラブ，インコーポレイテッド | 腸および血液脳関門の透過性を検出する方法、およびその試験材料 |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100357736C (zh) * | 2003-07-25 | 2007-12-26 | 大连康基生物技术有限公司 | 沙门氏菌快速检测装置 |
| CN103197078B (zh) * | 2013-03-28 | 2015-04-08 | 扬州大学 | 鸡白痢沙门菌分泌性蛋白SpiC的用途 |
| CN103995126B (zh) * | 2014-04-18 | 2015-10-14 | 中国农业大学 | 一种检测鸡白痢沙门氏菌抗体的elisa试剂盒 |
| CN104059142B (zh) * | 2014-07-03 | 2016-11-23 | 江南大学 | 一种用于制备沙门氏菌交叉型抗体的免疫原的合成方法 |
| CN104792991B (zh) * | 2015-04-17 | 2016-08-17 | 江南大学 | 一种基于单克隆抗体的检测食品中沙门氏菌属的特异性双抗体夹心法 |
| CN104726534B (zh) * | 2015-04-23 | 2017-04-19 | 河南省商业科学研究所有限责任公司 | 一种快速检测鲜乳中沙门氏菌的方法 |
| CN113125717B (zh) * | 2021-04-07 | 2024-02-13 | 江苏大学 | 基于微流控芯片的食物中沙门氏菌浓度检测方法与装置 |
| CN113968905B (zh) * | 2021-10-27 | 2024-08-02 | 哈尔滨国生生物科技股份有限公司 | 一种牛血清白蛋白及其应用 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4870158A (en) * | 1987-01-05 | 1989-09-26 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Polymyxin lipopolysaccharide antigen and associated method |
| US4906567A (en) * | 1987-01-21 | 1990-03-06 | E. I. Dupont De Nemours And Company | Non-immunochemical binding of lipopolysaccharides and sandwich assays therefor |
| JPH04270965A (ja) * | 1990-05-18 | 1992-09-28 | Burton W Blais | オリゴペプタイド吸着担体の調製方法、及びこれを使用したリポ多糖類の検定と除去方法 |
| US5976820A (en) * | 1995-08-28 | 1999-11-02 | Jolley; Michael E. | Detection of antibodies to bacterial antigens by flourescence polarization |
-
1998
- 1998-09-17 DE DE1998142609 patent/DE19842609C2/de not_active Expired - Fee Related
-
1999
- 1999-09-17 EP EP99955751A patent/EP1114321A2/fr not_active Withdrawn
- 1999-09-17 WO PCT/DE1999/003017 patent/WO2000017653A2/fr not_active Application Discontinuation
- 1999-09-17 JP JP2000571263A patent/JP2002525605A/ja not_active Withdrawn
- 1999-09-17 BR BR9913837-9A patent/BR9913837A/pt not_active IP Right Cessation
- 1999-09-17 CN CNB998108707A patent/CN1153971C/zh not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014503829A (ja) * | 2011-01-28 | 2014-02-13 | イミュノサイエンシス ラブ，インコーポレイテッド | 腸および血液脳関門の透過性を検出する方法、およびその試験材料 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP1114321A2 (fr) | 2001-07-11 |
| CN1153971C (zh) | 2004-06-16 |
| DE19842609C2 (de) | 2000-09-21 |
| DE19842609A1 (de) | 2000-04-27 |
| CN1318152A (zh) | 2001-10-17 |
| WO2000017653A2 (fr) | 2000-03-30 |
| BR9913837A (pt) | 2001-06-12 |
| WO2000017653A3 (fr) | 2000-05-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A300 | Application deemed to be withdrawn because no request for examination was validly filed |
Free format text: JAPANESE INTERMEDIATE CODE: A300 Effective date: 20061205 |