JP2002505590A - 新規な酵素のハイスループットスクリーニング - Google Patents

新規な酵素のハイスループットスクリーニング

Info

Publication number
JP2002505590A
JP2002505590A JP50478299A JP50478299A JP2002505590A JP 2002505590 A JP2002505590 A JP 2002505590A JP 50478299 A JP50478299 A JP 50478299A JP 50478299 A JP50478299 A JP 50478299A JP 2002505590 A JP2002505590 A JP 2002505590A
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JP
Japan
Prior art keywords
dna
cells
enzyme
cell
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
JP50478299A
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English (en)
Japanese (ja)
Other versions
JP2002505590A5 (https=
Inventor
ショート，ジェイ，エム．
ケラー，マーティン
Original Assignee
ディベルサ コーポレーション
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Application filed by ディベルサ コーポレーション filed Critical ディベルサ コーポレーション
Publication of JP2002505590A publication Critical patent/JP2002505590A/ja
Publication of JP2002505590A5 publication Critical patent/JP2002505590A5/ja
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP50478299A 1997-06-16 1998-06-16 新規な酵素のハイスループットスクリーニング Ceased JP2002505590A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/876,276 1997-06-16
US08/876,276 US20030215798A1 (en) 1997-06-16 1997-06-16 High throughput fluorescence-based screening for novel enzymes
PCT/US1998/012674 WO1998058085A1 (en) 1997-06-16 1998-06-16 High throughput screening for novel enzymes

Publications (2)

Publication Number Publication Date
JP2002505590A true JP2002505590A (ja) 2002-02-19
JP2002505590A5 JP2002505590A5 (https=) 2006-01-05

Family

ID=25367333

Family Applications (1)

Application Number Title Priority Date Filing Date
JP50478299A Ceased JP2002505590A (ja) 1997-06-16 1998-06-16 新規な酵素のハイスループットスクリーニング

Country Status (6)

Country Link
US (9) US20030215798A1 (https=)
EP (1) EP1009858A4 (https=)
JP (1) JP2002505590A (https=)
AU (1) AU749587B2 (https=)
CA (1) CA2294380A1 (https=)
WO (1) WO1998058085A1 (https=)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2005121336A1 (ja) * 2004-06-08 2008-04-10 東亞合成株式会社 環境ｄｎａの精製方法及び環境ｄｎａからのタンパク質をコードする遺伝子の効率的なスクリーニング方法
JP4829240B2 (ja) * 2004-10-12 2011-12-07 メディカル リサーチ カウンシル マイクロ流体制御による区画化されたスクリーニング
JP2013176393A (ja) * 2007-01-30 2013-09-09 Verenium Corp リグノセルロース性物質を処理する酵素、前記をコードする核酸並びに前記を製造及び使用する方法
JP2015092164A (ja) * 2003-03-31 2015-05-14 メディカル リサーチ カウンシル コンパートメント化スクリーニングによる選択
JP2017006145A (ja) * 2006-05-11 2017-01-12 レインダンス テクノロジーズ， インコーポレイテッド 微小流体デバイス
KR20200009926A (ko) * 2018-07-20 2020-01-30 한국생명공학연구원 이산화탄소 환원 활성이 증가된 포메이트 탈수소화 효소 스크리닝 시스템과 이의 용도

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* Cited by examiner, † Cited by third party
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