SE441009B - Sett att immobilisera levande biomaterial i perlformiga polymerer - Google Patents
Sett att immobilisera levande biomaterial i perlformiga polymererInfo
- Publication number
- SE441009B SE441009B SE8201401A SE8201401A SE441009B SE 441009 B SE441009 B SE 441009B SE 8201401 A SE8201401 A SE 8201401A SE 8201401 A SE8201401 A SE 8201401A SE 441009 B SE441009 B SE 441009B
- Authority
- SE
- Sweden
- Prior art keywords
- cells
- polymer
- aqueous solution
- pct
- agarose
- Prior art date
Links
- 229920000642 polymer Polymers 0.000 title claims abstract description 19
- 239000012620 biological material Substances 0.000 title abstract description 5
- 210000004027 cell Anatomy 0.000 claims abstract description 26
- 229920000936 Agarose Polymers 0.000 claims abstract description 12
- 210000004102 animal cell Anatomy 0.000 claims abstract description 10
- 239000011324 bead Substances 0.000 claims abstract description 8
- 102000008186 Collagen Human genes 0.000 claims abstract description 7
- 108010035532 Collagen Proteins 0.000 claims abstract description 7
- 239000005662 Paraffin oil Substances 0.000 claims abstract description 7
- 229920001436 collagen Polymers 0.000 claims abstract description 7
- 229960005188 collagen Drugs 0.000 claims abstract description 7
- DOIRQSBPFJWKBE-UHFFFAOYSA-N dibutyl phthalate Chemical compound CCCCOC(=O)C1=CC=CC=C1C(=O)OCCCC DOIRQSBPFJWKBE-UHFFFAOYSA-N 0.000 claims abstract description 6
- 108010049003 Fibrinogen Proteins 0.000 claims abstract description 5
- 102000008946 Fibrinogen Human genes 0.000 claims abstract description 5
- 229940012952 fibrinogen Drugs 0.000 claims abstract description 5
- 229920001817 Agar Polymers 0.000 claims abstract description 4
- 241000894006 Bacteria Species 0.000 claims abstract description 4
- 241000195493 Cryptophyta Species 0.000 claims abstract description 4
- 239000008272 agar Substances 0.000 claims abstract description 4
- 239000000679 carrageenan Substances 0.000 claims abstract description 4
- 229920001525 carrageenan Polymers 0.000 claims abstract description 4
- 235000010418 carrageenan Nutrition 0.000 claims abstract description 4
- 229940113118 carrageenan Drugs 0.000 claims abstract description 4
- 229920001296 polysiloxane Polymers 0.000 claims abstract description 4
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims abstract description 3
- 230000000717 retained effect Effects 0.000 claims abstract description 3
- STCOOQWBFONSKY-UHFFFAOYSA-N tributyl phosphate Chemical compound CCCCOP(=O)(OCCCC)OCCCC STCOOQWBFONSKY-UHFFFAOYSA-N 0.000 claims abstract description 3
- 239000007864 aqueous solution Substances 0.000 claims abstract 4
- 241000233866 Fungi Species 0.000 claims abstract 3
- 238000010438 heat treatment Methods 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 4
- 241001465754 Metazoa Species 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 2
- 239000006185 dispersion Substances 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 229920006395 saturated elastomer Polymers 0.000 claims description 2
- 150000001720 carbohydrates Chemical class 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 230000003100 immobilizing effect Effects 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 claims 1
- 239000000243 solution Substances 0.000 abstract description 9
- 241000196324 Embryophyta Species 0.000 abstract description 5
- 239000002612 dispersion medium Substances 0.000 abstract description 4
- 235000012424 soybean oil Nutrition 0.000 abstract description 4
- 239000003549 soybean oil Substances 0.000 abstract description 4
- 108010010803 Gelatin Proteins 0.000 abstract description 3
- 239000008273 gelatin Substances 0.000 abstract description 3
- 229920000159 gelatin Polymers 0.000 abstract description 3
- 229940014259 gelatin Drugs 0.000 abstract description 3
- 235000019322 gelatine Nutrition 0.000 abstract description 3
- 235000011852 gelatine desserts Nutrition 0.000 abstract description 3
- 238000005538 encapsulation Methods 0.000 abstract 2
- 229920001661 Chitosan Polymers 0.000 abstract 1
- 229940045110 chitosan Drugs 0.000 abstract 1
- 239000000499 gel Substances 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 description 7
- 239000000203 mixture Substances 0.000 description 6
- 230000001419 dependent effect Effects 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 108010088751 Albumins Proteins 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 102000009123 Fibrin Human genes 0.000 description 2
- 108010073385 Fibrin Proteins 0.000 description 2
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 2
- 238000004132 cross linking Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229950003499 fibrin Drugs 0.000 description 2
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 239000000178 monomer Substances 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 235000015112 vegetable and seed oil Nutrition 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 241000186359 Mycobacterium Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 108090000190 Thrombin Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003855 balanced salt solution Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 239000012894 fetal calf serum Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- OSVMTWJCGUFAOD-KZQROQTASA-N formestane Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)(C(CC4)=O)[C@@H]4[C@@H]3CCC2=C1O OSVMTWJCGUFAOD-KZQROQTASA-N 0.000 description 1
- 238000001879 gelation Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 229960004072 thrombin Drugs 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 235000019871 vegetable fat Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S424/00—Drug, bio-affecting and body treating compositions
- Y10S424/07—Microporous membranes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Public Health (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Dispersion Chemistry (AREA)
- Dermatology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Transplantation (AREA)
- Botany (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
- Medicinal Preparation (AREA)
- Artificial Fish Reefs (AREA)
Description
zs' 30 35 szoiaoiëo om det organiska lösningsmedlet utbyts mot endera av följande dispergeringsmedier: mättade eller omättade animaliska eller vegetabiliska fetter eller oljor, tri-n-butylfosfat, vätske- formig silikon, paraffin olja eller ftalsyradibutylester. Vissa av dessa dispergerinsmedier har använts förut för att framställa sfäriska polymerpartiklar. I US patent 4,l69,804 beskrivs en metod för att framställa magnetiska mikrosfärer genom att dispergera an albuminlösning i vegetabilisk olja och sedan upphetta denna till 14006 eller tvärbinda albuminpolymeren med aldehyder.
Denna teknik kan inte användas för att innsluta känsliga bio- material då antingen den höga temperaturen eller den tvärbindande aldehyden medför inaktivering.
I Biotechnology Letters vol. 3, 65-70, 1981 beskrivs en metod för att framställa sfäriska polyuretanpartiklar genom dispergering av monomererna i paraffinolja. Genom att inkludera bakterier i monomerblandningen erhölls en katalytisk aktiv preparation (huru- vida bakterierna har kvar sin förmåga att föröka sig är tveksamt, dessutom föreligger stor risk att de binds kovalent till bäraren).
Genom att kombinera dessa skonsamma dispergeringsmedier med en lämplig immobiliseringsmetod-bärare kan däremot såsom i de här beskrivna preparationerna en 100 %-ig viabilitet och bibehållen tillväxtförmåga erhållas. I tabell 1 jämförs olika dispergerings- mediers denaturerande verkan på agar-inneslutna växtceller. Av tabellen framgår att dessa dispergeringsmedier ger en bibehållen viabilitet jämfört med gängse standardmetod för framställning av sfäriska polymerpartiklar (svenskt patent 7204481-1).
Känsliga system såsom växtceller, alger eller animala celler innesluts lämpligen i polymerer såsom agar, agarose, karrageenan, gelatin, kollagen eller fibrinogen.
Animala celler förekommer i två principiellt skilda typer, suspensions- celler och så kallade ytberoende celler. De ytberoende cellerna måste vara fästade vid en yta för att kunna överleva och tillväxa.
I denna uppfinning används de bildade fibrerna av kollagen eller fibrin som nödvändig yta, detta medför att de ytberoende animala cellerna kan överleva och tillväxa i inneslutet-tillstånd. .___---_..-p~.__._.... . _. _ _ , 3 azo14o1-o Ytberoende celler kan inte tillväxa inneslutna i agarose, men genom att blanda agarose med fibrinogen eller kollagen kan även denna polymer användas för inneslutning av ytberoende celler.
Animala suspensionsceller har inte detta krav utan kan inneslutas i agarose, kollagen eller fibrin med bibehållen tillväxtförmâga.
Eftersom animala cellers potentiella användningsområde är mycket brett (produktion av vacciner, proteiner eller hormoner, trans- formering av prekursorer mm) är det viktigt att utveckla system där de kan användas i stor skala.
Inneslutna animala celler har stora produktionstekniska fördelar jämfört med fria celler, exempelvis kan de lätt användas i kolonn- processer, även i andra typer av reaktorer erhålls fördelar då de är mycket lättare att anpassa till kontinuerlig produktion.
Storleken av den bildade pärlan med inneslutet biomaterial kan varieras inom vida gränser beroende pâ med vilken kraft polymer- lösningen dispergeras. U szoiäoièo Exempel 1 _ Agar eller agarose löses i vatten (5.6 % w/v) genom uppvärmning.
Polymerlösningen (8 ml) tempereras till 500 C och blandas med växtceller (2 g) varefter dispergering i sojaolja (40 ml) sker. 5 När lagom stora kulor erhållits kyles blandningen till 50 C varefter kulorna tvättas över till vatten.
Exempel 2 Karrageenan löses i 0.9 % NaCl (3,1 % w/v) genom uppvärmning. 10 Kulor tillverkas enligt exempel 1.
Exempel 3 Gelatin löses i vatten genom uppvärmning (15 % w/v). Polymerlösningen (8 ml) tempereras till 370 C och blandas med jästceller (2 ml), 15 varefter blandningen dispergeras i sojaolja (40 ml). Efter av- kylning till 150 C tvättas kulorna över till vatten.
Exempel 4 Fibrinogenlösningen (1 ml, 2 % w/v) blandas med balanseradssàtt- 20 lösning (1 ml) samt fetalt kalvserum (D.3 ml). Till detta sätts animala celler (0,7 ml) samt trombin (1.5 U). Blandningen dispergeras i paraffinolja (40 ml). Efter avkylning tvättas kulorna över till odlingsmedium varefter de inneslutna cellerna odlas vid 370 C. 25 Exempel 5 Agarose löses i balanserad saltlösning (PBS) (5 % w/v) genom uppvärmning. Polymerlösningen (5 ml) tempereras till 37° C och _blandas med animala celler (5 ml), varefter blandningen dispergeras i paraffinolja (40 ml). Efter avkylning tvättas kulorna över till 30 medium och de inneslutna cellerna odlas vid 370 C.
Exempel 6 Kollagen löses i utspädd saltsyra. Polymerlösningen (kyld till 50 C, 2 ml) blandas med 10 ggr koncentrerat medium och natriumhydroxid 35 för att erhålla neutralt pH, samt animala celler (2 ml).
Blandningen dispergeras i paraffinolja (40 ml). Genom uppvärmning till 37° C sker gelning varefter kulorna tvättas över till medium och de inneslutna cellerna odlas vid 370 C. -__...._.....,._...____.. 10 15 20 820111014) Exempei 7 Hybridomceïier, LSP 21, innesiuts i agarose enïigt exempei 5.
Sïutïig ce11 koncentration 6 miijoner ceiier per m1 kuior.
De innesiutna ceiïerna används för produktion av monok1ona1a antikroppar. Resuitat eniigt tabe11 2.
Exempeï 8 Lymfobiastoida ceiier, MLA 144, innesiuts i agarose eniigt exempei 5. Siutiig ceii koncentration 20 miijoner ceiier per m1 kuior.
De innesïutna ceïierna används för produktion av Interieukin 2.
Resuitat enïigt tabeiï 2.
Exempei 9 Växtceiier, Catharantus roseus, innsiuts i agarose eniigt exempei 1. S1ut1ig ce11 koncentration 20 % (w/v). De innesïutna ceiïerna används för produktion av ajmaiicin.
Exempeï 10 - Bakterieceïïer, Mycobacterium, innesiuts i agarose eniigt exempei 1. Siutiig ceii koncentration 5 % (w/v). De innesiutna ceïierna producerade steroiden ¿J'4 omfattning som fritt suspenderade ce11er. -androstadione-3,17-dione i samma I _ " m'ïfifiaozzíjëfëe-ïiïíïïf vÄA.:.¿_j} 82011101-0 h Tabe11 1. Inverkan av hydrofob fas på agar-inneslutna växt- ce11ers respiration.
Hydrofob fas sojaolja trí-n-butyïfosfat paraffínoïja síïíkonvätska fta1syradíbuty1ester Reïatív respiration 100 100 91 100 82 tou1uene:k1oroform (73:27 v/v), Ar1ace1 83 ( % w/v) 0 8201401-0 | oil mv 1 03.. 2 v .. omm m wmm | ß | omm? w Nwm ommw m NPm 1 oæfll v mmm coop m www os; N mmm . omm» F mnflfiømmmufl xoumfiown Hmvflu man uwfifiwv FN mmq wavøflmmccfl øwE ummmonx Hmflumv :l mAS mdudfimmcøfi vw... lfiunm mfimcofixocoä >m :ofiuxøwonm N Gflxøufiuoßcfl >ß :ofluxøwoum .HÜHMÜU .NHNEHGM .NCUDHWÜCCM >fl .HmvfiWÅÜHOEOHQ xrfl ÉOfiHMUUOHN u N .HHÜQWB
Claims (4)
1. Förfarande för immobilisering av levande animala celler, växtceller, bakterier, alger eller svampar med bibehållen tillväxtförmåga genom inneslutning i polymerpärlor, av att cellerna sätts till en vattenlösning k ä n n e- t e c k n a t av en kolhydratbaserad eller proteinbaserad denna vattenlösning därefter dispergeras i ett vattenolöa- polymer, att ligt dispergeringsmedium, som utgöres av en mättad eller omättad vetetabilisk olja, tri-n-butylfosfat, vätskeformig silikon, paraffinolja eller ftalsyradibutylester, och att polymeren bringas att gela under bildning av polymerpärlor, vari cellerna innesluts,genom avkylning, neutralisering följd av uppvärmning eller enzymatisk påverkan att cellernas tillväxtförmâga icke förstörs. under sådana betingelser,
2. Förfarande enligt patentkravet 1, k ä n n e t e c k- n a t av att polymeren väljes bland agar, agaros, karrageenan, kollagen och fíbrinogen.
3. Pörfarande enligt något av de föregående patentkraven, c k n a t av att dispergeringen avbryts när företrädes- k ä n n e t e pärlorna erhållit en kornstorlek av 0,05 - 3 mm, vis 0,1 ~ 1,0 mm.
4. Förfarande enligt något av de föregående immobiliserade animala patentkraven, k ä n n e t e c k n a t av att de cellerna, växtcellerna, bakteriecellerna, algerna eller svamparna kan bringas att tillväxa genom tillförsel av nâringsmedium. i . _ _. ...__ ._..._..._-..__._.-......._,...,....___..__._._ _
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE8201401A SE441009B (sv) | 1982-03-08 | 1982-03-08 | Sett att immobilisera levande biomaterial i perlformiga polymerer |
| AT83900856T ATE32348T1 (de) | 1982-03-08 | 1983-03-08 | Verfahren zum immobilisieren von biomaterial in kugelpolymeren. |
| JP58500933A JPS59500350A (ja) | 1982-03-08 | 1983-03-08 | ビ−ドポリマ−による生物学的物質のカプセル化法 |
| US06/552,044 US4647536A (en) | 1982-03-08 | 1983-03-08 | Method of encapsulating biomaterial in bead polymers |
| PCT/SE1983/000074 WO1983003102A1 (en) | 1982-03-08 | 1983-03-08 | A method of encapsulating bio material in bead polymers |
| EP83900856A EP0102985B1 (en) | 1982-03-08 | 1983-03-08 | A method of immobilizing bio material in bead polymers |
| DE8383900856T DE3375581D1 (en) | 1982-03-08 | 1983-03-08 | A method of immobilizing bio material in bead polymers |
| DK509483A DK509483A (da) | 1982-03-08 | 1983-11-07 | Fremgangsmaade til inkorporering af biomateriale i perleformige polymerer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE8201401A SE441009B (sv) | 1982-03-08 | 1982-03-08 | Sett att immobilisera levande biomaterial i perlformiga polymerer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SE8201401L SE8201401L (sv) | 1983-09-09 |
| SE441009B true SE441009B (sv) | 1985-09-02 |
Family
ID=20346185
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE8201401A SE441009B (sv) | 1982-03-08 | 1982-03-08 | Sett att immobilisera levande biomaterial i perlformiga polymerer |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US4647536A (sv) |
| EP (1) | EP0102985B1 (sv) |
| JP (1) | JPS59500350A (sv) |
| AT (1) | ATE32348T1 (sv) |
| DE (1) | DE3375581D1 (sv) |
| DK (1) | DK509483A (sv) |
| SE (1) | SE441009B (sv) |
| WO (1) | WO1983003102A1 (sv) |
Families Citing this family (122)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4643968A (en) * | 1981-01-29 | 1987-02-17 | Massachusetts Institute Of Technology | Process for determining metabolism and growth of cells under various conditions |
| DE3376660D1 (en) * | 1983-06-22 | 1988-06-23 | Stolle Res & Dev | Encapsulated cells, their method of preparation and use |
| DE3584610D1 (de) * | 1984-09-21 | 1991-12-12 | Cernitin Sa | Lebende mikroorganismen enthaltendes fluessiges praeparat, verfahren zu dessen herstellung, lebende mikroorganismen enthaltendes pelletiertes produkt und verfahren zu dessen herstellung. |
| US5629191A (en) * | 1985-01-03 | 1997-05-13 | Integra Lifesciences Corporation | Method of making a porous matrix particle |
| US4871671A (en) * | 1985-04-29 | 1989-10-03 | Minnesota Mining And Manufacturing Company | Immobilization of biological cells in polytetrafluoroethylene matrix |
| US4997443A (en) * | 1985-08-26 | 1991-03-05 | Hana Biologics, Inc. | Transplantable artificial tissue and process |
| GB8523327D0 (en) * | 1985-09-20 | 1985-10-23 | Atomic Energy Authority Uk | Cells |
| SE464816B (sv) * | 1985-10-15 | 1991-06-17 | Nilsson Kjell | Makroporoesa partiklar, foerfarande foer dess framstaellning och dess anvaendning |
| US5314814A (en) * | 1985-11-15 | 1994-05-24 | Gist-Brocades | Preparation of novel immobilized biocatalysts |
| PT83746B (pt) * | 1985-11-15 | 1988-08-17 | Gist Brocades Nv | Processo para a preparacao de novos biocatalisadores imobilizados e para a producao de etanol por fermentacao |
| WO1987004458A1 (fr) * | 1986-01-25 | 1987-07-30 | Nitta Gelatin Inc. | Procede de culture de cellules animales a grande echelle |
| US4789545A (en) * | 1986-03-31 | 1988-12-06 | New York Blood Center, Inc. | Removal of lipid soluble process chemicals from biological materials by extraction with naturally occurring oils or synthetic substitutes thereof |
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|---|---|---|---|---|
| BE789195A (fr) * | 1971-09-24 | 1973-03-22 | Gist Brocades Nv | Compositions d'enzymes |
| SE393115B (sv) * | 1972-04-07 | 1977-05-02 | K Mosbach | Sett att immobilisera enzymer genom inneslutning i polymernetverk med hjelp av perlpolymerisationsprocessen |
| JPS5312994B2 (sv) * | 1974-06-15 | 1978-05-06 | ||
| SE427116B (sv) * | 1976-07-06 | 1983-03-07 | Larsson Per Olof | Transformation av steroider och antibiotika med immobiliserade levande mikroorganismer som aktiveras |
| US4169804A (en) * | 1976-08-19 | 1979-10-02 | Minnesota Mining And Manufacturing Company | Magnetically responsive composite microparticle |
| DE2805607C3 (de) * | 1978-02-10 | 1980-08-28 | 3300 Braunschweig | Herstellung von Bio-Katalysatoren durch Polymereinschluß von Mikroorganismen |
| US4352883A (en) * | 1979-03-28 | 1982-10-05 | Damon Corporation | Encapsulation of biological material |
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| US4353888A (en) * | 1980-12-23 | 1982-10-12 | Sefton Michael V | Encapsulation of live animal cells |
| US4518693A (en) * | 1982-11-01 | 1985-05-21 | Research Corporation | Immobilized biocatalysts |
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1982
- 1982-03-08 SE SE8201401A patent/SE441009B/sv not_active IP Right Cessation
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1983
- 1983-03-08 AT AT83900856T patent/ATE32348T1/de not_active IP Right Cessation
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- 1983-03-08 DE DE8383900856T patent/DE3375581D1/de not_active Expired
- 1983-03-08 EP EP83900856A patent/EP0102985B1/en not_active Expired
- 1983-03-08 US US06/552,044 patent/US4647536A/en not_active Expired - Lifetime
- 1983-03-08 JP JP58500933A patent/JPS59500350A/ja active Pending
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|---|---|
| DK509483D0 (da) | 1983-11-07 |
| DE3375581D1 (en) | 1988-03-10 |
| US4647536A (en) | 1987-03-03 |
| SE8201401L (sv) | 1983-09-09 |
| WO1983003102A1 (en) | 1983-09-15 |
| ATE32348T1 (de) | 1988-02-15 |
| DK509483A (da) | 1983-11-07 |
| EP0102985B1 (en) | 1988-02-03 |
| EP0102985A1 (en) | 1984-03-21 |
| JPS59500350A (ja) | 1984-03-08 |
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