JP2002241300A - Skin care preparation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a skin care preparation containing the extract of Phellinus linteus carpophore. SOLUTION: This skin care preparation is characterized by containing the extract of Phellinus linteus carpophore. The extract has excellent histamine liberation inhibitory activity, hyaluronidase inhibitory activity and tyrosinase inhibitory activity; therefore this skin care preparation is excellent in rough skin ameliorative activity, wrinkle preventive activity and bleaching activity.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an external preparation for skin which is excellent in improving rough skin, preventing wrinkles and whitening.

[0002]

2. Description of the Related Art Skin irritation due to ultraviolet rays or the like is one of the causes of skin roughness, and an external preparation for skin which reduces the inflammation and improves the skin roughness is desired. As one of the treatment methods, there is a method of suppressing release of histamine, which is a proinflammatory substance generated during inflammation. Conventionally, for the treatment of rough skin, treatment using topical steroids, indomethacin, and the like has been performed.

[0003] Hyaluronidase is activated during inflammation and reduces the molecular weight of high-molecular-weight hyaluronic acid.
It is known that the firmness of the skin is reduced to cause senile changes such as wrinkles.

[0004] Ascorbic acid is generally known as a skin whitening external preparation used for spots and freckles.

[0005] On the other hand, fruiting bodies of Mesimakobu are generally used as crude drugs or health foods having an antitumor effect.
Japanese Patent Application Laid-Open No. 61-129113 discloses the use of a mycelial culture instead of a fruiting body as a bath agent.

[0006]

Since steroids and the like conventionally used as histamine release inhibitors have a risk of side effects, a natural external skin preparation having high safety and excellent effect is desired. I have. Further, ascorbic acid used as a skin whitening agent for external use has a problem that it is easily decomposed with time, and therefore, a skin external agent derived from a natural product having high stability and excellent effect is also desired. In addition, there is a need for safe and stable hyaluronidase inhibitors.

From the above, it is safe and excellent in stability,
An external preparation for skin is desired for improving rough skin, preventing wrinkles, and whitening.

[0008]

[Means for Solving the Problems] In view of such circumstances,
The present inventors have conducted intensive studies and found that the extract of fruit body of Mesimakobu has excellent histamine release action (anti-inflammatory action), hyaluronidase activity inhibitory action and tyrosinase activity inhibitory action, and is also excellent in stability. I found that. Furthermore, they found that an external preparation for skin containing the extract was safe and stable, and was excellent in improvement of rough skin, prevention of wrinkles and whitening effect, and completed the present invention.

[0009] Meshimakobu (scientific name: Phelli) used in the present invention
nus linteus) is a basidiomycete belonging to the genus Bombyx mori, which is a natural product or a cultivated product, and can be obtained in markets such as Japan, Korea, and China.

The extract of Mesamakobu used in the present invention is:
It is extracted from the fruiting body of Meshimakobu. The extraction method is not particularly limited, and may be, for example, the one extracted by heating or the one extracted at room temperature.

Examples of the solvent to be extracted include water, lower alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, 1-butanol).
Heptanol, 2-heptanol, etc.), liquid polyhydric alcohols (propylene glycol, glycerin, 1,3-butylene glycol, etc.), ketones (acetone, methyl ethyl ketone, etc.), acetonitrile, esters (ethyl acetate,
Butyl acetate, etc.), hydrocarbons (hexane, heptane, liquid paraffin, etc.), and ethers (ethyl ether, propyl ether, tetrahydrofuran, etc.).
Preferred are polar solvents such as water, lower alcohols and liquid polyhydric alcohols, and particularly preferred are water, ethanol, propylene glycol and 1,3-butylene glycol. These solvents may be used alone or in combination of two or more.

[0012] The extract of Mesimakobu may be used as it is as an extracted solution, or may be used after being subjected to treatment such as concentration, dilution, and filtration as needed. Further, the extracted solution may be subjected to a treatment such as concentration and drying, spray drying, and freeze drying to be used as a dried product.

The above-mentioned extract may be used as it is in the external preparation for skin of the present invention, and oils and fats, waxes, and the like, which are components used in the external preparation, as long as the effect of the extract is not impaired.
Hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, fragrances, humectants, powders, ultraviolet absorbers, thickeners, pigments, antioxidants,
Components such as whitening agents and chelating agents can be blended.

The external preparation for skin of the present invention can be used in any of cosmetics, quasi-drugs, and pharmaceuticals. Examples of the dosage form include lotions, creams, emulsions, gels, aerosols, and the like. Ointments, cataplasms, essences, packs, detergents, bath preparations, foundations, powders, lipsticks and the like applied to the skin can be mentioned.

The amount of Mesimakobu extract used in the present invention is calculated by converting the total amount of the external preparation for skin of the present invention to a solid matter.
0.0001% by weight or more, preferably 0.001 to 10% by weight is good. If it is less than 0.0001% by weight, a sufficient effect is hardly expected. If the content is more than 10% by weight, the effect is hardly recognized and it is uneconomical. In addition, the method of addition may be added in advance or may be added during the production,
What is necessary is just to select suitably in consideration of workability.

[0016]

EXAMPLES In order to explain the present invention in detail, examples of the production of the extract used in the present invention, prescription examples of the present invention and experimental examples will be given below, but the present invention is not limited to these examples. Absent. The term “parts by weight” in Examples means “parts by weight”, and “%” means “% by weight”.

Production Example 1 Hot water extract of Mesimakobu 400 mL of purified water was added to 20 g of the dried fruit body of Mesimakov,
After extraction at 100100 ° C. for 2 hours, the mixture was filtered, and the filtrate was concentrated and freeze-dried to obtain 0.86 g of a hot water extract.

Production Example 2 Ethanol Extract of Mesimakobu 2 g of ethanol was added to 200 g of fruiting bodies of Mesimakobu, and the mixture was added at room temperature.
After extraction for one day, the mixture was filtered, and the filtrate was concentrated to dryness to obtain 2.2 g of an ethanol extract.

Production Example 3 Extract of 50% 1,3-butylene glycol of Mesimakobu 200 mL of purified water and 200 ml of 1,3-butyleneglycol were added to 20 g of fruiting bodies of Mesimakobu, extracted at room temperature for 7 days, filtered and extracted. 380 g was obtained.

Example 1 Lotion Formulation Formulation amount 1. Hot water extract of meshimakobu (Production Example 1) 0.1 part 2. 1,3-butylene glycol 8.0 3. Glycerin 2.0 4. Xanthan gum 0.02 5. Citric acid 0.01 6. Sodium citrate 0.1 7. Ethanol 5.0 8. Methyl parahydroxybenzoate 0.1 9. Polyoxyethylene hydrogenated castor oil (40E.O.) 0.1 10. Perfume appropriate amount 11. Make the total amount 100 with purified water. 1 to 6 and 11 and the components 7 to 10 are uniformly dissolved, respectively, and both are mixed and filtered to obtain a product.

COMPARATIVE EXAMPLE 1 Conventional lotion The conventional lotion was prepared by replacing the hot water extract of Mesimakob with purified water in Example 1.

Example 2 Cream Formulation Formulation amount 1. Ethanol extract of meshimakobu (Production Example 2) 0.05 part 2. Squalane 5.5 3. Olive oil 3.0 4. Stearic acid 2.0 5. Beeswax 2.0 6. Octyldodecyl myristate 3.5 7. Polyoxyethylene cetyl ether (20E.O.) 3.0 8. Behenyl alcohol 1.5 9. Glycerin monostearate 2.5 10. Fragrance 0.1 11. Methyl parahydroxybenzoate 0.2 12. Ethyl paraoxybenzoate 0.05 13. 1,3-butylene glycol 8.5 14. Adjust the total amount to 100 with purified water. [Production method] Heat and dissolve components 2 to 9 and mix. Maintain at 70 ° C to obtain an oil phase. Components 1 and 11 to 14 are dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. Add the water phase to the oil phase, emulsify, cool with stirring, add component 10 at 45 ° C,
Cool to 30 ° C to obtain a product.

Comparative Example 2 Conventional Cream A conventional cream was prepared by replacing the ethanol extract of Mesimacob with purified water in Example 2.

Example 3 Emulsion Formulation Compounding amount 1. Hot water extract of Mesimakobu (Preparation Example 1) 0.001 part 2. Squalane 5.0 3. Olive oil 5.0 4. Jojoba oil 5.0 5. Cetanol 1.5 6. Glycerin monostearate 2.0 7 Polyoxyethylene cetyl ether (20E.O.) 3.0 8. Polyoxyethylene sorbitan monooleate (20E.O.) 2.0 9. Fragrance 0.1 10. Propylene glycol 1.0 11. Glycerin 2.0 12. Methyl paraoxybenzoate 0.2 13 Adjust the total amount to 100 with purified water. [Production method] Heat and dissolve components 2 to 8 and keep at 70 ° C to obtain an oil phase. Components 1 and 10 to 13 are dissolved by heating and mixed, and the mixture is kept at 75 ° C. to form an aqueous phase. Add the water phase to the oil phase, emulsify, cool with stirring, add component 9 at 45 ° C, and add
Cool to 0 ° C to obtain a product.

Example 4 Gel Formulation Formulation Content 1. 50% 1,3-butylene glycol extract of meshimakobu (Preparation Example 3) 1.0 part 2. Ethanol 5.0 3. Methyl parahydroxybenzoate 0.1 4. Polyoxyethylene cured castor Oil (60E.O.) 0.1 5. Appropriate amount of fragrance 6. 1,3-butylene glycol 5.0 7. Glycerin 5.0 8. Xanthan gum 0.1 9. Carboxyvinyl polymer 0.2 10. Potassium hydroxide 0.2 11. Purified water to 100 [Production method] Components 2 to 5, and components 1 and 6 to 11 are each dissolved uniformly, and both are mixed to obtain a product.

Example 5 Ointment Prescription Formulation amount 1. Hot water extract of meshimakobu (Production Example 1) 0.01 part 50% 1,3-butylene glycol extract of meshimakobu (Production Example 3) 0.5 3. Polyoxyethylene cetyl ether (30E.O.) 2.0 4. Glycerin monostearate 10.0 5. Liquid paraffin 5.0 6. Cetanol 6.0 7. Methyl parahydroxybenzoate 0.1 8. Propylene glycol 10.0 9. Make the total amount up to 100 with purified water. [Production method] Heat and dissolve components 3 to 6 and keep at 70 ° C to obtain an oil phase. Components 1, 2 and 7 to 9 are dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. Add the water phase to the oil phase, emulsify and cool to 30 ° C with stirring to obtain the product.

Example 6 Pack Prescription Formulation Amount 1. Hot water extract of Mesimakobu (Production Example 1) 0.1 part Ethanol extract of meshimakobu (Production Example 2) 0.1 3. Polyvinyl alcohol 12.0 4. Ethanol 5.0 5. 1,3-butylene glycol 8.0 6. Methyl paraoxybenzoate 0.2 7. Polyoxyethylene hydrogenated castor oil (20E.O.) 0.5 8. Citric acid 0.1 9. Sodium citrate 0.3 10. Appropriate amount of fragrance 11. Make the total amount 100 with purified water.

Example 7 Foundation Prescription Formulation amount 1. Ethanol extract of meshimakobu (Production Example 2) 1.0 part 2. Stearic acid 2.4 3. Polyoxyethylene sorbitan monostearate (20E.O.) 1.0 4. Polyoxyethylene Cetyl ether (20E.O.) 2.0 5. Cetanol 1.0 6. Liquid lanolin 2.0 7. Liquid paraffin 3.0 8. Isopropyl myristate 6.5 9. Butyl paraoxybenzoate 0.1 10. Sodium carboxymethylcellulose 0.1 11. Bentonite 0.5 12. Propylene glycol 4.0 13. Triethanolamine 1.1 14. Methyl paraoxybenzoate 0.2 15. Titanium dioxide 8.0 16. Talc 4.0 17. Bengala 1.0 18. Yellow iron oxide 2.0 19. Perfume Appropriate amount 20. Adjust the total amount to 100 with purified water. Swell the ingredient 10 well to the ingredient 20, followed by the ingredient
Add 1 and 11-14 and mix uniformly. Add the ingredients 15 to 18 crushed and mixed with a crusher, and stir with a homomixer.
Keep at 75 ° C to make the aqueous phase. Add the oil phase to this aqueous phase while stirring, cool, add component 19 at 45 ° C, and stir
Cool to 30 ° C to obtain a product.

Example 8 Bath agent Prescription Compounding amount 1. Hot water extract of meshimakobu (Production Example 1) 5.0 parts 2. Sodium hydrogen carbonate 50.0 3. Yellow 202 (1) qs 4. Fragrance qs 5. Sodium sulfate [Production method] Components 1 to 5 are uniformly mixed to obtain a product.

Next, experimental examples will be described in order to explain the effects of the present invention in detail.

EXPERIMENTAL EXAMPLE 1 Histamine release inhibitory action (anti-inflammatory action) Using Production Examples 1 and 2 as samples, male Spraque-Dawley
The effect of inhibiting histamine release was measured using mast cells collected from the abdominal cavity of rats. That is, 1 μg
The effect of inhibiting the release of histamine from mast cells by the 48/80 compound / ml was determined as the release inhibition rate. Mast cells were obtained according to the method of Sullivan et al. [J. Immunology, 114, 1473 (19
75)], and the amount of histamine was determined by the method of May et al. [J.
Allergy, 46, 12 (1970)]. In addition, indomethacin was used as a positive control.

The results of these experiments are shown in Table 1. As a result, the extract of Mesimakobu of Production Examples 1 and 2 showed an excellent action of suppressing the release of histamine.

[0033]

[Table 1]

Experimental Example 2 Hyaluronidase Inhibiting Activity (Anti-aging Effect) The extracts of Production Examples 1, 2 and 3 were tested for hyaluronidase inhibitory activity by applying the Morgan-Elson method [Food Hygiene Magazine, 31, 3 (1990). ]. That is, 175 mL of 0.1 M acetate buffer (pH 4.0) was added to the sample solution, and the enzyme activity of hyaluronidase was 400 U / mL, the concentration of hyaluronic acid was 0.4 mg / mL, and the compound 48/80 of the activator was added to 0.0
After adjusting the total amount to 500 mL so as to be 6 mg / mL, a hyaluronidase reaction was performed at 37 ° C. for 40 minutes. After the reaction, a color was developed by adding a p-dimethylaminobenzaldehyde reagent, and the absorbance at 585 nm was measured. In addition, the inhibitory effect of each sample was calculated by the inhibition rate obtained from the following equation. As a control, purified water was used instead of the sample, and purified water was used instead of hyaluronidase as a blank. Inhibition rate (%) = [1- (CD) / (AB)] × 10
0 A: Absorbance at 585 nm of control (OD585) B: OD585 of control blank C: OD585 of sample D: OD585 of sample blank

Table 2 shows the results of these experiments. As a result, the Mesimakobu extracts of Production Examples 1 and 2 exhibited excellent hyaluronidase inhibitory activity.

[0036]

[Table 2]

Experimental Example 3 Tyrosinase inhibitory action (whitening action) Using Production Examples 1 and 2 as samples, the tyrosinase inhibitory action was measured. In a test tube, 0.2 mL of the sample solution and 1 mL of the L-tyrosine solution (0.3 mg / mL) and McBane's buffer (pH
6.8) After adding 0.6 mL and leaving the mixture at 37 ° C. for 10 minutes, 0.2 mL of a 3,000 U / mL tyrosinase aqueous solution was added thereto and mixed well. After 3 minutes, the absorbance at 475 nm was measured.
The inhibitory effect of each sample was calculated by the inhibition rate calculated from the following equation. In addition, purified water was used instead of the sample for the control,
Purified water was used in place of tyrosinase as a blank. Inhibition rate (%) = [1- (CD) / (AB)] × 10
0 A: absorbance at 475 nm of control (OD475) B: OD475 of control blank C: OD475 of sample D: OD475 of sample blank

The results of these experiments are shown in Table 3. As a result, the Mesimakobu extract of Production Examples 1 and 2 showed an excellent tyrosinase inhibitory action. In addition, ascorbic acid used as a positive control had a reduced tyrosinase inhibitory effect when left at 40 ° C. for 2 weeks, whereas the extract of Mesimacob did not change its inhibitory effect. From the above, it was confirmed that the extract of Mesimakobu had a stable and excellent tyrosinase inhibitory action.

[0039]

[Table 3]

EXPERIMENTAL EXAMPLE 4 Use Test 1 Using the lotion of Example 1, the cream of Example 2, the conventional lotion of Comparative Example 1, and the conventional cream of Comparative Example 2, 30 women suffering from rough skin (21 (Age 46 years old). After use, the effect of improving skin roughness was determined by a questionnaire. Table 4 shows the results. As a result, an external preparation for skin characterized by containing an extract of Mesimakobu showed excellent action for improving rough skin. In addition, all monitors with improved skin roughness using the lotion of Example 1 and the cream of Example 2 answered that skin firmness was improved and wrinkles were less noticeable. During the test period, there was no skin trouble and there was no problem in safety. Also, there was no problem with the deterioration of the prescription components.

[0041]

[Table 4]

Experimental Example 5 Use test 2 15 women suffering from spots and freckles using the lotion of Example 1, the cream of Example 2, the conventional lotion of Comparative Example 1, and the conventional cream of Comparative Example 2. (21-46 years old) was subjected to a one-month use test. After use, the effect of improving spots and freckles was determined by a questionnaire. Table 5 shows the results. As a result, the external preparation for skin characterized by containing the extract of Mesimakobu showed excellent stain and freckle-improving action. During the test period, there was no skin trouble and there was no problem in safety. Also, there was no problem with the deterioration of the prescription components.

[0043]

[Table 5]

The use test was carried out similarly for the emulsion of Example 3, the gel of Example 4, the ointment of Example 5, the pack of Example 6, the foundation of Example 7, and the bath agent of Example 8. It exhibited excellent skin roughness improvement, wrinkle prevention, and amelioration of spots and freckles.

[0045]

As described above, the extract of fruiting body of Mesimakobu of the present invention has excellent histamine release inhibitory action, hyaluronidase inhibitory action and tyrosinase inhibitory action, and was stable. Furthermore, the external preparation for skin characterized by containing the extract of the fruit body of Mesimakobu was highly safe and exhibited excellent improvement of rough skin, prevention of wrinkles, and improvement of spots and freckles (whitening action).

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification code FI Theme coat ゛ (Reference) A61P 43/00 111 A61P 43/00 111 (72) Inventor Satoru Nakata 2-7 Tomicho, Nishi-ku, Nagoya Menard Japan F-term (reference) in Cosmetics Co., Ltd. 4C083 AA082 AA111 AA122 AB032 AB232 AB242 AB312 AB352 AB432 AB442 AC022 AC072 AC102 AC122 AC182 AC242 AC302 AC352 AC422 AC432 AC442 AC482 AC542 AD092 AD112 AD272 AD352 AD512 CC04 CC05 CC07 CC12 CC12 DD2241 EE41C AA02 AC17 CA05 CA06 MA28 NA14 ZA89 ZC20 ZC41

Claims (4)

[Claims] 1. An external preparation for skin, comprising an extract of fruit body of Mesimakobu. 2. A histamine release inhibitor comprising an extract of fruit body of Mesimakobu. 3. A hyaluronidase activity inhibitor comprising an extract of a fruit body of Mesimakobu. 4. A whitening agent comprising an extract of fruit body of Mesimakobu.