JP2001278767A - Cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a cosmetic which has a cell proliferation-stimulating action and an anti-aging action and has excellent effects for improving skins having freckles, wrinkles, darkness and the like. SOLUTION: This cosmetic is obtained by dissolving in water at least either one of (B) a salt obtained by acidifying sea water, adding a basic solution to the acidic sea water, removing the produced precipitates, concentrating the obtained solution, cooling the concentrated solution, and then filtering the product, and (C) a salt obtained by evaporating the solvent from the filtrate.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a cosmetic composition containing a salt obtained from seawater to prevent skin aging such as wrinkles and spots or to promote cell proliferation.

[0002]

2. Description of the Related Art Human skin gradually ages with aging, and it is known that various external stimuli other than aging cause skin aging. In particular, ultraviolet rays in sunlight cause cross-linking and cutting of the components of the extracellular matrix, such as collagen and elastin, thereby reducing the elasticity and moisturizing properties of the skin. Cause. The skin that has undergone such a change becomes more susceptible to external stimuli, which leads to a vicious cycle in which cell activity is reduced and the regenerative power of the extracellular matrix is reduced, and aging proceeds rapidly. The loss of skin gloss, the increase in spots, dullness, etc., which progresses in a short period of time, are often caused by the strong external stimulus as described above. Therefore, in order to prevent skin aging due to external irritation, it has been practiced to blend an ultraviolet absorber or a humectant into the cosmetic.

[0003] In addition, an ingredient which inhibits collagenase and elastase is blended into cosmetics to maintain the extracellular matrix in a normal state, and vitamin C and vitamin E
It has also been practiced to blend a component having a cell growth promoting action such as

[0004] However, it is difficult to completely block harmful ultraviolet rays with an ultraviolet absorber that can be blended normally.
If it is used in a large amount, irritation to the skin becomes a problem. It is also difficult to sufficiently activate skin cells with a humectant, a collagenase inhibitor or the like, and cell growth promoting components such as vitamin C have problems in safety, stability and the like, and have not always been satisfactory.

[0005]

An object of the present invention is to contain a salt obtained from seawater, to prevent skin aging such as wrinkles and spots, and to promote cell proliferation, thereby reducing irritation.
It is to provide cosmetics with high safety.

[0006]

Means for Solving the Problems As a result of intensive studies to solve the above problems, the present inventors have completed a cosmetic according to the present invention having an antiaging effect and / or a cell growth promoting effect. It led to.

More specifically, claim 1 relates to a cosmetic containing a salt (A) obtained from a solution obtained by removing a generated precipitate by adding a basic solution after acidifying seawater.

A second aspect of the present invention is a salt obtained by acidifying seawater, adding a basic solution to remove a generated precipitate, concentrating the resulting solution, cooling, and filtering the solution. The present invention relates to a cosmetic containing at least one of B) and a salt (C) obtained by evaporating a solvent from a filtered solution.

A third aspect of the present invention relates to a cosmetic containing an aqueous solution obtained by dissolving a salt (A) obtained from a solution from which a generated precipitate has been removed by adding a basic solution after acidifying seawater.

A fourth aspect of the present invention relates to a salt obtained by acidifying seawater, adding a basic solution to remove a generated precipitate, concentrating the obtained solution, cooling, and filtering the solution. The present invention relates to a cosmetic containing an aqueous solution in which at least one of B) and a salt (C) obtained by evaporating a solvent from a filtered solution is dissolved in water.

[0011] Claim 5 relates to a cosmetic characterized in that the salt (A) is contained in an amount of 0.1 to 2.0% by weight.

[0012] Claim 6 relates to a cosmetic characterized in that the total amount of both the salt (B) and the salt (C) is 0.1 to 2.0% by weight.

[0013] Claim 7 relates to a cosmetic characterized by having at least one of an antiaging effect and a cell growth promoting effect.

The cosmetic of the present invention can be in the form of any cosmetic applied to the skin, such as lotions, emulsions, creams, packs, gel cosmetics, powder cosmetics, soaps, and liquid detergents. .

The cosmetics of the present invention include, among quasi-drugs defined by the Pharmaceutical Affairs Law, hair growth agents, bath agents, medicinal cosmetics, medicinal cosmetics, ie, medicinal soaps, medicinal shampoos,
Medicated rinse, medicated lotion, medicated cream / milk, medicated pack, hair dye, hair cosmetics, general cream / milk, shaving cream or lotion, tanning / sunscreen lotion, cosmetic oil, ointment, tanning / sunscreen oil, It can be applied to white powder, powder, foundation, perfume, pack, nail cream, enamel, enamel remover, eyebrow ink, blush, eye cream, eye shadow, mascara, eyeliner, lipstick, lip balm, brush, bath cosmetics and the like.

[0016] The cosmetic of the present invention comprises constituents usually used in the production of the cosmetic, such as oils, surfactants,
Thickeners, humectants, preservatives, fragrances, colorings, chelating agents,
It may contain one or more amino acids, saccharides and the like.

Furthermore, the cosmetic of the present invention may contain, if necessary, an arbitrary amount of glycyrrhizic acid or a salt thereof or a derivative thereof (such as stearyl glycyrrhetinate), allantoin, azulene, baicalin, marronnier extract, and oakaku. Extract, spinach extract, ascorbic acid, magnesium ascorbate phosphate, ellagic acid,
Selected from arbutin, kojic acid, placenta extract, mulberry bark acid, kudin extract, sprouts extract, oedum extract, vitamins, tocopherol and its derivatives, natural vitamin E, collagen, elastin / hyaluronic acid, chondroitin sulfate It may contain one or more components.

[0018] Claim 8 is a method for producing the salt (A) which is a component contained in cosmetics, wherein the first step of acidifying seawater and the addition of a basic solution are performed to remove precipitates formed. And a third step of producing the salt (A) from the solution obtained in the second step.
The present invention relates to a method for producing a salt (A) which is a component of a cosmetic.

A ninth aspect of the present invention is a method for producing a salt (B) or a salt (C) which is a component contained in a cosmetic, wherein the first step of acidifying seawater and the step of adding a basic solution are carried out. A second step of removing the precipitate formed, and concentrating and cooling the solution obtained in the second step to produce the salt (B) obtained by filtration, or And a third step of producing the salt (C) obtained by evaporating the solvent from the solution thus obtained, which relates to a method for producing the salt (B) or the salt (C), which is a component contained in cosmetics.

A tenth aspect of the present invention is a method for producing a cosmetic, comprising: a first step of acidifying seawater; a second step of adding a basic solution to remove generated precipitates; The present invention relates to a method for producing a cosmetic, comprising: a third step of producing a salt (A) from the solution obtained in the step of (a); and a fourth step of incorporating the salt (A) into the cosmetic.

[0021] Claim 11 is a method for producing a cosmetic, comprising: a first step of acidifying seawater; a second step of adding a basic solution to remove generated precipitates; The solution obtained in the step is concentrated and cooled, followed by filtration to produce the salt (B) obtained, or the salt obtained by evaporating the solvent from the filtered solution (C) And a fourth step in which at least one of the salt (B) and the salt (C) is contained in a cosmetic.

[0022]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in more detail with reference to examples, but the following examples do not limit the present invention.

To 500 liters of seawater, 10 liters of an aqueous solution in which 5% concentrated sulfuric acid was added to an aqueous solution in which calcium phosphate was dissolved to remove precipitates was added. After standing for 3 hours, insolubles were removed by filtration. As a result, seawater is p
It was H1.6. Next, 15 kg of sodium hydroxide was added to 500 liters of low pH seawater, and the mixture was allowed to stand for 10 hours. At this time, the formed precipitate was separated by filtration, and 10 liters of the remaining seawater was heated to remove water to obtain a 1.5 liter concentrated solution. The concentrated solution is quenched to form a precipitate, and the salt (B) obtained by filtration is
The salt (C) obtained by drying the solution after filtration was combined to obtain 200 g. An undiluted solution was obtained by dissolving 100 g of the mixture of the salt (B) and the salt (C) (hereinafter referred to as “cell growth promoting substance”) in water.

[0024]

Examples 1 to 3,

Comparative Examples 1 and 2 Cosmetics were produced from the following (a) to (c) as raw materials by a conventional method. (A) (1) 4% by weight of dipropylene glycol (2) 1,3-butylene glycol 2 (3) polyoxyethylene (60) hydrogenated castor oil 1 (4) ethyl alcohol 4 (5) methyl paraoxybenzoate 1 (6) Citric acid 0.2 (7) Sodium citrate 0.1 (8) Fragrance 0.02 (b) See Table 1 (c) Make the total amount 100 with purified water.

[0025]

[Table 1]

[0026]

Test Example 1 The cell lotion of Examples 1 to 3 and Comparative Examples 1 and 2 were tested for cell growth promoting action by the following MTT method.

The MTT method (J. immunol. Method 93, 157.198)
6): Pre-cultured human normal neonatal dermal fibroblasts (N
B1RGB) was dispensed into a 96-well microplate, and a 10% FBS culture solution-added RITC80-7 medium (Farto Pharmaceutical Co., pH 7.2) in which the sample was dissolved was added at 37 ° C. under 5% CO _2.
Incubate for days. Next, the MTT reagent (3- (4,5-dimethyl-2)
-thiazolyl) -2,5-diphenyl-2H tetrazolium bromide, 5m
g / ml) and incubate for 4.5 hours (the active enzyme derived from mitochondria in the proliferated cells reacted with the MTT reagent and the yellow color of the reagent was 57%).
It turns blue-yellow with an absorption peak at 0 mm). Thereafter, the absorbance (A) at 570 mm is measured using a microplate reader. Separately, the same culture is performed without adding a sample, and the absorbance (B) is measured. Further, the absorbance (C) when a sample is added and the same treatment is performed without adding cells is measured. Finally, the absorbance (D) when the same treatment is performed without adding any sample or cells is also measured.

Each absorbance measurement was taken at 650 measured simultaneously.
The absorbance in mm is subtracted to correct for the effect of increased turbidity by the grown cells. From the corrected absorbances, the cell growth promotion rate is determined by the following equation. Cell growth promotion rate (%) = [{(AC) − (BD)}
/ (BD)] × 100 The results are as shown in Table 2, and the cell growth promoting effect was confirmed for the lotions of all Examples, and particularly excellent cell proliferation was obtained for the lotions of Examples 1 to 3. A promoting effect was confirmed. However, the lotions of Comparative Examples 1 and 2 did not show a cell growth promoting effect.

[0029]

[Table 2] Cell growth promotion rate (%) (Note: “Sample concentration” is the sample concentration in Medium 1.)

[0030]

Example 4 A cream was produced by treating the following ingredients in a conventional manner. (1) Cell growth promoting substance 2.0 (2) Acetostearyl alcohol 3.5 (3) Squalane 30.0 (4) Beeswax 3.0 (5) Reduced lanolin 5.0 (6) Stearyl paraoxybenzoate 0. 1 (7) Polyoxyethylene (20) sorbitan monostearate 2.0 (8) Glycerin monostearate 5.0 (9) 1,3-butylene glycol 5.0 (10) Fragrance 0.05 (11) The total amount is made 100 with purified water.

[0031]

Comparative Example 3 A cream was produced by removing the cell growth promoting substance from the raw materials of Example 4.

[0032]

[Test Example 2] Woman 1 complaining of skin wrinkles, wrinkles and dryness
The following use test was performed on the creams of Example 4 and Comparative Example 3 with eight panelists (age 33 to 45 years: those who scored 1 and 2 in both items according to the following criteria).

Test method: The panelists were divided into two groups of nine persons, one of which received the cream of Example 4 and the other of which received the cream of Comparative Example 3 twice daily for 50 days, twice daily in the morning and evening.
It was applied to the face. Fifty days later, the number and depth of gloss, blemishes and squats, and wrinkles on the coated surface were visually observed, and the scores were answered according to the following criteria. [Skin Gloss / Kusumi and Kasatsu] Score Observation Results 1 There is no skin gloss and the skin is strong. Exfoliation of the stratum corneum is often observed. 2 There is no skin gloss, there is dark spots on the whole, and exfoliation of the stratum corneum is seen in some places. 3 The skin is slightly shiny and no dark spots are seen. The stratum corneum does not peel off with the naked eye, but it peels off when rubbed lightly with a cotton swab. 4 The skin is shiny and firm. There is almost no peeling of the stratum corneum even when rubbed with cosmetic cotton. [Wrinkles] Score Observation result 1 Many wrinkles are found at the corners of the eyes. 2 There are wrinkles at the corners of the eyes, and the number is slightly large. 3 There are wrinkles at the corners of the eyes, but the number is small. 4 No wrinkles at the corners of the eyes are observed with a normal expression. The test results are shown in Tables 3 and 4.

[0034]

[Table 3] Improving effect of skin gloss, blemishes and Kasatsuki

[0035]

[Table 4] Wrinkle improvement effect

[0036]

Example 5 An emulsion having the following composition was produced by a conventional method. (1) Cell growth promoting substance 2.0 (2) Carrot extract 0.065 (3) Cetyl alcohol 0.5 (4) Squalane 3.0 (5) Beeswax 1.0 (6) Vaseline 2.0 (7) Polyoxyethylene (20) sorbitan monooleate 1.0 (8) Glycerin monostearate 1.0 (9) Dipropylene glycol 4.0 (10) Ethanol 2.0 (11) Methyl valaoxybenzoate 12 (12) Sodium hyaluronate 0.1 (13) Purified water to make the total amount 100.

[0037]

Comparative Example 4 An emulsion was prepared by removing the cell growth promoting substance from the raw materials of Example 5.

[0038]

Test Example 3 The same test as in Test Example 2 was performed. The test results are shown in Tables 5 and 6.

[0039]

[Table 5] Improvement effect of skin gloss, blemishes and scabs

[0040]

[Table 6] Improvement effect of wrinkles

[0041]

Example 6 A pack having the following composition was produced by a conventional method. (1) Cell growth promoting substance 1.0 (2) Polyvinyl alcohol 15.0 (3) Polyethylene glycol 3.0 (4) Propylene glycol 7.0 (5) Ethanol 10.0 (6) Methyl paraben 0.05 (7) Perfume 0.05 (8) Make up to 100 with purified water.

[0042]

Comparative Example 5 A pack was prepared by removing the cell growth promoting substance from the raw materials of Example 6.

[0043]

Test Example 4 The same test as in Test Example 2 was performed. The test results are shown in Tables 7 and 8.

[0044]

[Table 7] The effect of improving skin gloss, dark spots and sickness

[0045]

[Table 8] Wrinkle improvement effect

[0046]

Example 7 An ointment having the following composition was produced by a conventional method. (1) Cell growth promoting substance 0.5 (2) Cetyl alcohol 18.0 (3) Mokuro 20.0 (4) Polyoxyethylene (20) monooleate 0.25 (5) Glycerin monostearate 0 .25 (6) Vaseline 40.0 (7) Make up to 100 with purified water.

[0047]

Comparative Example 6 An ointment was produced by removing the cell growth promoting substance from the raw materials of Example 7.

[0048]

Test Example 5 The same test as in Test Example 2 was performed. Tables 9 and 10 show the test results.

[0049]

[Table 9] Improving effect of skin gloss, blemishes and Kasatsuki

[0050]

[Table 10] Wrinkle improvement effect

From the above test results 3-5, Examples 5-7
In each of the examples, the skin improvement effects such as gloss, spots, wrinkles, dullness, etc. were found to be extremely superior to Comparative Examples 4 to 6 which did not contain the cell growth promoting substance.

[0052]

Industrial Applicability According to the present invention, there is provided a cosmetic which has an effect of promoting cell proliferation and an effect of preventing aging and is effective for improving skin such as spots, wrinkles and dullness.

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (reference) A61K 35/08 A61K 35/08 A61P 43/00 107 A61P 43/00 107 F term (reference) 4C083 AA082 AA112 AA122 AA161 AA162 AC012 AC022 AC072 AC102 AC122 AC302 AC402 AC422 AC432 AC442 AC482 AD042 AD112 AD332 AD512 CC02 CC05 CC07 DD22 DD31 EE12 4C087 AA01 AA02 AA04 BA06 CA01 MA63 NA14 ZA89 ZB22

Claims (11)

[Claims] 1. A cosmetic containing a salt (A) obtained from a solution in which seawater is acidified and then a basic solution is added to remove a generated precipitate. 2. After acidifying the seawater, adding a basic solution to remove a precipitate formed, concentrating the obtained solution, cooling, and filtering to remove the salt (B) obtained by filtration. ,
A cosmetic containing at least one of a salt (C) obtained by evaporating a solvent from a solution separated by filtration. 3. A cosmetic containing an aqueous solution obtained by dissolving a salt (A) obtained from a solution from which a precipitate has been removed by adding a basic solution after acidifying seawater to water. 4. After acidifying seawater, adding a basic solution to remove a precipitate formed, concentrating the obtained solution, cooling, and filtering to remove a salt (B) obtained by filtration. ,
A cosmetic containing an aqueous solution in which at least one of a salt (C) obtained by evaporating a solvent from a filtered solution and water is dissolved in water. 5. The cosmetic according to claim 1, wherein the salt (A) contains 0.1 to 2.0% by weight. 6. The cosmetic according to claim 2, wherein the total amount of both the salt (B) and the salt (C) is 0.1 to 2.0% by weight. 7. The method according to claim 1, which has at least one of an anti-aging effect and a cell growth promoting effect.
The cosmetic according to any one of claims 1 to 6. 8. A method for producing a salt (A) which is a component contained in a cosmetic, comprising: a first step of acidifying seawater; and a second step of adding a basic solution to remove a generated precipitate. And a third step of producing the salt (A) from the solution obtained in the second step. A method for producing a salt (A) which is a component of a cosmetic, comprising: 9. A method for producing a salt (B) or a salt (C) which is a component contained in a cosmetic, comprising: a first step of acidifying seawater; and a precipitate formed by adding a basic solution. And a solution obtained by concentrating and cooling the solution obtained in the second step to produce the salt (B) obtained by filtering, or by filtering the solution. And a third step of producing the salt (C) obtained by evaporating a solvent from the above. A method for producing a salt (B) or a salt (C) which is a component contained in a cosmetic, comprising: 10. A method for producing a cosmetic, comprising: a first step of acidifying seawater; a second step of adding a basic solution to remove a generated precipitate; and the second step. A method for producing a cosmetic, comprising: a third step of generating a salt (A) from the obtained solution; and a fourth step of including the salt (A) in the cosmetic. 11. A method for producing a cosmetic, comprising: a first step of acidifying seawater; a second step of adding a basic solution to remove a generated precipitate; and the second step. The resulting solution is concentrated, cooled and filtered to yield a salt (B), or the solvent is evaporated from the filtered solution to yield a salt (C). A method for producing a cosmetic, comprising: a third step; and a fourth step of causing the cosmetic to contain at least one of the salt (B) and the salt (C).