IT1261847B - Metodo per marcare cellule eucariote. - Google Patents
Metodo per marcare cellule eucariote.Info
- Publication number
- IT1261847B IT1261847B ITRM930587A ITRM930587A IT1261847B IT 1261847 B IT1261847 B IT 1261847B IT RM930587 A ITRM930587 A IT RM930587A IT RM930587 A ITRM930587 A IT RM930587A IT 1261847 B IT1261847 B IT 1261847B
- Authority
- IT
- Italy
- Prior art keywords
- receptor
- marking
- nucleic acid
- cell surface
- cell
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 210000004027 cell Anatomy 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 102000005962 receptors Human genes 0.000 abstract 3
- 108010001857 Cell Surface Receptors Proteins 0.000 abstract 1
- 108091026890 Coding region Proteins 0.000 abstract 1
- 241000124008 Mammalia Species 0.000 abstract 1
- 210000003527 eukaryotic cell Anatomy 0.000 abstract 1
- 238000001415 gene therapy Methods 0.000 abstract 1
- 230000003834 intracellular effect Effects 0.000 abstract 1
- 239000003446 ligand Substances 0.000 abstract 1
- 102000006240 membrane receptors Human genes 0.000 abstract 1
- 230000019491 signal transduction Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/71—Receptors; Cell surface antigens; Cell surface determinants for growth factors; for growth regulators
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Urology & Nephrology (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Un metodo per marcare una cellula eucariota (mammifero) esprimendo in questa cellula un acido nucleico, detto acido nucleico codificante un recettore di superficie cellulare, e successivamente presentando detto recettore alla superficie cellulare, caratterizzato dal fatto di utilizzare un acido nucleico in cui la regione codificante il dominio intracellulare del recettore è stata completamente o parzialmente deleta o modificata in maniera tale che il recettore presentato alla superficie cellulare non può, dopo legame al suo ligando, effettuare alcuna trasduzione di segnale, è efficace e utilizzabile nella terapia genica.
Priority Applications (14)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ITRM930587A IT1261847B (it) | 1993-09-01 | 1993-09-01 | Metodo per marcare cellule eucariote. |
JP7507213A JP2781276B2 (ja) | 1993-09-01 | 1994-08-11 | マーカーとしての細胞表面レセプターの使用による真核細胞のマーキング方法 |
PT94926187T PT724634E (pt) | 1993-09-01 | 1994-08-11 | Metodo para a marcacao de celulas eucarioticas por meio da utilizacao de um receptor da superficie da celula como um marcador |
DE69433342T DE69433342T2 (de) | 1993-09-01 | 1994-08-11 | Methode zur markierung von eukaryotischen zellen mittels verwendung von rezeptoren der zelloberfläche als marker |
DK94926187T DK0724634T3 (da) | 1993-09-01 | 1994-08-11 | Fremgangsmåde til mærkning af eukaryote celler ved anvendelsen af en celleoverfladereceptor som markør |
ES94926187T ES2210260T3 (es) | 1993-09-01 | 1994-08-11 | Metodo de marcado de celulas eucarioticas empleando como marcador un receptor de superficie celular. |
CA002170757A CA2170757C (en) | 1993-09-01 | 1994-08-11 | Method of marking eukaryotic cells by the use of a cell surface receptor as a marker |
PCT/EP1994/002687 WO1995006723A1 (en) | 1993-09-01 | 1994-08-11 | Method of marking eukaryotic cells by the use of a cell surface receptor as a marker |
US08/602,791 US6074836A (en) | 1993-09-01 | 1994-08-11 | Method of marking eukaryotic cells |
EP94926187A EP0724634B1 (en) | 1993-09-01 | 1994-08-11 | Method of marking eukaryotic cells by the use of a cell surface receptor as a marker |
AT94926187T ATE254660T1 (de) | 1993-09-01 | 1994-08-11 | Methode zur markierung von eukaryotischen zellen mittels verwendung von rezeptoren der zelloberfläche als marker |
IL147557A IL147557A (en) | 1993-09-01 | 1994-08-29 | Method for the immunoselection and immunoseparation of transfected eukaryotic cells |
IL11081594A IL110815A0 (en) | 1993-09-01 | 1994-08-29 | Method of marking eukaryotic cells |
IL14755702A IL147557A0 (en) | 1993-09-01 | 2002-01-10 | Method for the immunoselection and immunoseparation of transfected eukaryotic cells |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ITRM930587A IT1261847B (it) | 1993-09-01 | 1993-09-01 | Metodo per marcare cellule eucariote. |
Publications (3)
Publication Number | Publication Date |
---|---|
ITRM930587A0 ITRM930587A0 (it) | 1993-09-01 |
ITRM930587A1 ITRM930587A1 (it) | 1995-03-01 |
IT1261847B true IT1261847B (it) | 1996-06-03 |
Family
ID=11401929
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ITRM930587A IT1261847B (it) | 1993-09-01 | 1993-09-01 | Metodo per marcare cellule eucariote. |
Country Status (12)
Country | Link |
---|---|
US (1) | US6074836A (it) |
EP (1) | EP0724634B1 (it) |
JP (1) | JP2781276B2 (it) |
AT (1) | ATE254660T1 (it) |
CA (1) | CA2170757C (it) |
DE (1) | DE69433342T2 (it) |
DK (1) | DK0724634T3 (it) |
ES (1) | ES2210260T3 (it) |
IL (2) | IL110815A0 (it) |
IT (1) | IT1261847B (it) |
PT (1) | PT724634E (it) |
WO (1) | WO1995006723A1 (it) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19643682C1 (de) * | 1996-10-23 | 1998-01-15 | Manfred Prof Dr Gareis | Verfahren zum Herkunftsnachweis von Nutztieren sowie davon stammenden Produkten |
US6500421B1 (en) * | 1996-11-04 | 2002-12-31 | St. Jude Children's Research Hospital | In vivo selection of primitive hematopoietic cells |
JP4080544B2 (ja) | 1997-01-31 | 2008-04-23 | 株式会社ディナベック研究所 | ノックアウト動物 |
EP0904786B1 (en) * | 1997-08-22 | 2004-12-15 | Science Park Raf S.p.A. | Tumor vaccination by use of autologous or HLA-related antigen presenting cell (APC) transduced with a tumour antigen and a foreign antigen capable of causing an immune reaction |
ZA989497B (en) | 1997-10-20 | 2000-04-19 | Roche Diagnostics Gmbh | Positive-negative selection in homologous recombination. |
IT1297016B1 (it) * | 1997-12-23 | 1999-08-03 | San Raffaele Centro Fond | Processo di selezione di linfociti t cd4+ per la transduzione di geni anti-hiv |
IT1312570B1 (it) * | 1999-05-28 | 2002-04-22 | Genera Spa | Metodo per il traferimento di antigeni a cellule dendritiche. |
US6790614B1 (en) | 1999-11-19 | 2004-09-14 | Novartis Ag | Selectable cell surface marker genes |
WO2001036659A2 (en) * | 1999-11-19 | 2001-05-25 | Novartis Ag | Selectable cell surface marker genes |
JP2003533183A (ja) * | 2000-03-30 | 2003-11-11 | ノバルティス アクチエンゲゼルシャフト | 選択マーカー遺伝子 |
DE10019075B4 (de) * | 2000-04-18 | 2007-01-18 | Vision 7 Gmbh | Verwendung von CD34 oder einem davon abgeleiteten Polypeptid als Zell-Oberflächen- bzw. Gentransfer-Marker |
EP1390384A4 (en) * | 2001-05-30 | 2005-08-17 | Chromos Molecular Systems Inc | PLATFORMS ON CHROMOSOME BASIS |
US7737124B2 (en) * | 2001-09-13 | 2010-06-15 | California Institute Of Technology | Method for expression of small antiviral RNA molecules with reduced cytotoxicity within a cell |
US7919309B2 (en) | 2001-09-13 | 2011-04-05 | California Institute Of Technology | Method for expression of small antiviral RNA molecules within a cell |
AU2003241409A1 (en) * | 2003-05-12 | 2005-01-21 | Potomac Pharmaceuticals, Inc. | Gene expression suppression agents |
GB0413702D0 (en) | 2004-06-18 | 2004-07-21 | Molmed Spa | Thymidine kinase |
JP4956427B2 (ja) * | 2004-07-21 | 2012-06-20 | デイナ ファーバー キャンサー インスティチュート,インコーポレイテッド | レンチウイルスベクターおよびその使用 |
AU2012320480A1 (en) | 2011-10-05 | 2014-04-24 | Molmed Spa | Viral vectors purification system |
US20140037599A1 (en) * | 2012-08-03 | 2014-02-06 | The Trustees Of The University Of Pennsylvania | Compositions and Methods of Treating T Cell Deficiency |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5055459A (en) * | 1986-06-30 | 1991-10-08 | Board Of Regents, The University Of Texas | Selective elimination of malignant cells from bone marrow by bis (acyloxy) propylphosphoramidates |
AU7583891A (en) * | 1990-04-17 | 1991-11-11 | United States of America, as represented by the Secretary, U.S. Department of Commerce, The | Selective cytotoxic reagents comprising toxic moieties derived from mammalian proteins |
CA2040099A1 (en) * | 1990-05-01 | 1991-11-02 | Mariano Barbacid | Tyrosine kinase negative trkb |
US5462856A (en) * | 1990-07-19 | 1995-10-31 | Bunsen Rush Laboratories, Inc. | Methods for identifying chemicals that act as agonists or antagonists for receptors and other proteins involved in signal transduction via pathways that utilize G-proteins |
ATE129746T1 (de) * | 1990-09-14 | 1995-11-15 | Univ Johns Hopkins | Verfahren und zusammensetzungen für gentherapie und stärkung des immunsystems. |
US5470730A (en) * | 1990-09-28 | 1995-11-28 | Immunex | Method for producing TH -independent cytotoxic T lymphocytes |
JP3150340B2 (ja) * | 1990-11-13 | 2001-03-26 | イムネクス コーポレイション | 二機能選択可能融合遺伝子 |
AU9164991A (en) * | 1990-11-30 | 1992-06-25 | Abbott Laboratories | Immunoassay and monoclonal antibodies useful for detecting truncated nerve growth factor receptor |
AU1922092A (en) * | 1991-05-03 | 1992-12-21 | Albert Einstein College Of Medicine Of Yeshiva University | Targeted delivery of genes encoding cell surface receptors |
US5529774A (en) * | 1991-08-13 | 1996-06-25 | The Regents Of The University Of California | In vivo transfer of the HSV-TK gene implanted retroviral producer cells |
JP2006344159A (ja) * | 2005-06-10 | 2006-12-21 | Toshiba Information Systems (Japan) Corp | 共通バス接続デバイス用通信制御装置 |
-
1993
- 1993-09-01 IT ITRM930587A patent/IT1261847B/it active IP Right Grant
-
1994
- 1994-08-11 DE DE69433342T patent/DE69433342T2/de not_active Expired - Lifetime
- 1994-08-11 DK DK94926187T patent/DK0724634T3/da active
- 1994-08-11 EP EP94926187A patent/EP0724634B1/en not_active Expired - Lifetime
- 1994-08-11 PT PT94926187T patent/PT724634E/pt unknown
- 1994-08-11 ES ES94926187T patent/ES2210260T3/es not_active Expired - Lifetime
- 1994-08-11 US US08/602,791 patent/US6074836A/en not_active Expired - Lifetime
- 1994-08-11 CA CA002170757A patent/CA2170757C/en not_active Expired - Lifetime
- 1994-08-11 AT AT94926187T patent/ATE254660T1/de active
- 1994-08-11 JP JP7507213A patent/JP2781276B2/ja not_active Expired - Lifetime
- 1994-08-11 WO PCT/EP1994/002687 patent/WO1995006723A1/en active IP Right Grant
- 1994-08-29 IL IL11081594A patent/IL110815A0/xx not_active IP Right Cessation
- 1994-08-29 IL IL147557A patent/IL147557A/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
EP0724634B1 (en) | 2003-11-19 |
JPH09501569A (ja) | 1997-02-18 |
DE69433342T2 (de) | 2004-09-09 |
JP2781276B2 (ja) | 1998-07-30 |
EP0724634A1 (en) | 1996-08-07 |
CA2170757A1 (en) | 1995-03-09 |
DE69433342D1 (de) | 2003-12-24 |
PT724634E (pt) | 2004-03-31 |
ES2210260T3 (es) | 2004-07-01 |
WO1995006723A1 (en) | 1995-03-09 |
IL110815A0 (en) | 1994-11-11 |
US6074836A (en) | 2000-06-13 |
ITRM930587A1 (it) | 1995-03-01 |
CA2170757C (en) | 2003-04-22 |
ITRM930587A0 (it) | 1993-09-01 |
DK0724634T3 (da) | 2004-03-29 |
IL147557A (en) | 2006-10-31 |
ATE254660T1 (de) | 2003-12-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
0001 | Granted | ||
TA | Fee payment date (situation as of event date), data collected since 19931001 |
Effective date: 19970929 |