IN2014DN00221A - - Google Patents

Download PDF

Info

Publication number
IN2014DN00221A
IN2014DN00221A IN221DEN2014A IN2014DN00221A IN 2014DN00221 A IN2014DN00221 A IN 2014DN00221A IN 221DEN2014 A IN221DEN2014 A IN 221DEN2014A IN 2014DN00221 A IN2014DN00221 A IN 2014DN00221A
Authority
IN
India
Prior art keywords
target polynucleotide
strands
double stranded
polynucleotide
stranded target
Prior art date
Application number
Other languages
English (en)
Inventor
Clive Brown
James Clarke
Graham Hall
Gavin Harper
Andrew Heron
James White
Original Assignee
Oxford Nanopore Tech Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford Nanopore Tech Ltd filed Critical Oxford Nanopore Tech Ltd
Publication of IN2014DN00221A publication Critical patent/IN2014DN00221A/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811Selection methods for production or design of target specific oligonucleotides or binding molecules
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44717Arrangements for investigating the separated zones, e.g. localising zones
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44756Apparatus specially adapted therefor
    • G01N27/44791Microapparatus
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/48707Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721Investigating individual macromolecules, e.g. by translocation through nanopores

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
IN221DEN2014 2011-07-25 2012-07-25 IN2014DN00221A (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161511436P 2011-07-25 2011-07-25
PCT/GB2012/051786 WO2013014451A1 (en) 2011-07-25 2012-07-25 Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores

Publications (1)

Publication Number Publication Date
IN2014DN00221A true IN2014DN00221A (de) 2015-06-05

Family

ID=46763140

Family Applications (1)

Application Number Title Priority Date Filing Date
IN221DEN2014 IN2014DN00221A (de) 2011-07-25 2012-07-25

Country Status (10)

Country Link
US (7) US9957560B2 (de)
EP (1) EP2737084B1 (de)
JP (1) JP6298404B2 (de)
KR (1) KR20140050067A (de)
CN (1) CN103827320B (de)
AU (1) AU2012288629B2 (de)
BR (1) BR112014001699A2 (de)
CA (1) CA2843136C (de)
IN (1) IN2014DN00221A (de)
WO (1) WO2013014451A1 (de)

Families Citing this family (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102216783B (zh) 2008-09-22 2015-04-01 华盛顿大学 Msp纳米微孔和相关方法
CN102369298B (zh) 2009-01-30 2017-03-22 牛津纳米孔技术有限公司 跨膜测序中用于核酸构建体的衔接体
AU2012264497B2 (en) 2011-05-27 2017-06-15 Oxford Nanopore Technologies Limited Coupling method
IN2014DN00221A (de) 2011-07-25 2015-06-05 Oxford Nanopore Tech Ltd
US20160162634A1 (en) 2011-09-23 2016-06-09 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
EP2987870B1 (de) 2011-10-21 2020-02-19 Oxford Nanopore Technologies Limited Verfahren zur kennzeichnung eines zielpolynukleotids mit einer transmembranpore und eines molekularen motors
CN104136631B (zh) 2011-12-29 2017-03-01 牛津纳米孔技术公司 使用xpd解旋酶表征多核苷酸的方法
WO2013098562A2 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Enzyme method
US9238836B2 (en) 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
WO2013121201A1 (en) 2012-02-15 2013-08-22 Oxford Nanopore Technologies Limited Aptamer method
WO2013123450A1 (en) * 2012-02-16 2013-08-22 Genia Technologies, Inc Methods for creating bilayers for use with nanopore sensors
CA2866587C (en) 2012-02-16 2022-07-26 Oxford Nanopore Technologies Limited Analysis of measurements of a polymer
EP2836506B1 (de) 2012-04-10 2017-04-19 Oxford Nanopore Technologies Limited Lysenin mutant poren
WO2013185137A1 (en) 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
EP2875154B1 (de) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB-Verfahren zur Charakterisierung einer Nukleinsäure
EP2875128B8 (de) 2012-07-19 2020-06-24 Oxford Nanopore Technologies Limited Modifizierte helikasen
US9551023B2 (en) 2012-09-14 2017-01-24 Oxford Nanopore Technologies Ltd. Sample preparation method
WO2014072703A1 (en) 2012-11-06 2014-05-15 Oxford Nanopore Technologies Limited Quadruplex method
WO2014074922A1 (en) * 2012-11-09 2014-05-15 Stratos Genomics, Inc. Concentrating a target molecule for sensing by a nanopore
GB201222928D0 (en) 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
EP2958927A4 (de) * 2013-02-20 2016-09-14 Eve Biomedical Inc Verfahren und zusammensetzungen zur nanostrukturbasierten nukleinsäuresequenzierung
GB201318465D0 (en) * 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
EP2964779B1 (de) * 2013-03-08 2018-08-29 Oxford Nanopore Technologies Limited Verwendung von spacer-elementen in einer nukleinsäure zur kontrolle der bewegung einer helicase
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CN105899678A (zh) 2013-10-18 2016-08-24 牛津纳米孔技术公司 经修饰的酶
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
WO2015110813A1 (en) 2014-01-22 2015-07-30 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
EP3108229A4 (de) 2014-02-19 2017-09-06 University of Washington Nanoporenbasierte analyse von proteineigenschaften
GB201403096D0 (en) * 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US10337060B2 (en) 2014-04-04 2019-07-02 Oxford Nanopore Technologies Ltd. Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
CN106459159B (zh) * 2014-05-02 2021-11-30 牛津纳米孔技术公司 突变孔
GB201417712D0 (en) 2014-10-07 2014-11-19 Oxford Nanopore Tech Ltd Method
EP3169800B1 (de) 2014-07-14 2020-11-18 Oxford University Innovation Limited Messung von analyten mit membrankanalmolekülen und zweischichtige anordnungen
CN117164684A (zh) 2014-09-01 2023-12-05 弗拉芒区生物技术研究所 突变csgg孔
US10421998B2 (en) 2014-09-29 2019-09-24 The Regents Of The University Of California Nanopore sequencing of polynucleotides with multiple passes
WO2016055778A1 (en) 2014-10-07 2016-04-14 Oxford Nanopore Technologies Limited Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
KR102551897B1 (ko) 2014-10-16 2023-07-06 옥스포드 나노포어 테크놀로지즈 피엘씨 폴리머의 분석
US10480026B2 (en) 2014-10-17 2019-11-19 Oxford Nanopore Technologies Ltd. Method for nanopore RNA characterisation
GB201418469D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Method
WO2016077313A1 (en) 2014-11-11 2016-05-19 Bgi Shenzhen Multi-pass sequencing
EP3253889A4 (de) * 2015-02-02 2018-09-26 Two Pore Guys, Inc. Nanoporendetektion von zielpolynukleotiden aus einer probe
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
DE112016000293T5 (de) * 2015-02-26 2017-09-21 Hitachi High-Technologies Corporation Verfahren zum konstruieren eines nucleinsäure-moleküls
US11169138B2 (en) 2015-04-14 2021-11-09 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
IL285795B (en) * 2015-08-12 2022-07-01 Univ Hong Kong Chinese Sequencing a single molecule of DNA. plasma
US10280411B2 (en) 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
JP2018536416A (ja) * 2015-12-08 2018-12-13 クアンタポール, インコーポレイテッド 核酸をナノポアを通じて移動する方法
AU2016369071B2 (en) 2015-12-08 2022-05-19 Katholieke Universiteit Leuven Ku Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
CN116200476A (zh) 2016-03-02 2023-06-02 牛津纳米孔科技公开有限公司 靶分析物测定方法、突变CsgG单体及其构筑体、及聚核苷酸和寡聚孔
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
CA3212147A1 (en) 2016-04-06 2017-10-12 Oxford Nanopore Technologies Plc Mutant pore
GB201609221D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
JP2019517252A (ja) 2016-05-31 2019-06-24 クアンタポール, インコーポレイテッド 二色ナノポア配列決定
US10577653B2 (en) * 2016-06-27 2020-03-03 Roche Sequencing Solutions, Inc. Counteracting osmotic imbalance in a sequencing cell
GB201612458D0 (en) * 2016-07-14 2016-08-31 Howorka Stefan And Pugh Genevieve Membrane spanning DNA nanopores for molecular transport
EP3500684A4 (de) 2016-08-19 2020-02-26 Quantapore Inc. Optische nanoporensequenzierung mit quenchern
GB201620450D0 (en) 2016-12-01 2017-01-18 Oxford Nanopore Tech Ltd Method
WO2018104908A2 (en) 2016-12-09 2018-06-14 Boreal Genomics, Inc. Linked ligation
CA3053086A1 (en) 2017-02-10 2018-08-16 Oxford Nanopore Technologies Limited Modified nanopores, compositions comprising the same, and uses thereof
GB201707140D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
CN111065917A (zh) * 2017-06-21 2020-04-24 奥特拉公司 双孔-控制和传感器设备
KR20200030070A (ko) 2017-06-30 2020-03-19 브이아이비 브이지더블유 신규한 단백질 포어
GB2589159B (en) 2017-12-29 2023-04-05 Clear Labs Inc Nucleic acid sequencing apparatus
CN112041462A (zh) * 2018-03-23 2020-12-04 德克萨斯州立大学董事会 具有极低的错误水平的dsDNA有效测序
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
GB201809323D0 (en) 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
EP3815092A2 (de) 2018-06-29 2021-05-05 F. Hoffmann-La Roche AG Nachweis von mikrosatelliteninstabilität
GB201814369D0 (en) 2018-09-04 2018-10-17 Oxford Nanopore Tech Ltd Method for determining a polymersequence
EP3650558A1 (de) * 2018-11-07 2020-05-13 Siemens Healthcare GmbH Arbeitsablauf zur nanoporensequenzierung von flüssigproben
CA3120359A1 (en) * 2018-11-19 2020-05-28 The Regents Of The University Of California Methods for detecting and sequencing a target nucleic acid
GB201819378D0 (en) 2018-11-28 2019-01-09 Oxford Nanopore Tech Ltd Analysis of nanopore signal using a machine-learning technique
EP3884071A4 (de) * 2019-01-03 2022-04-06 Boreal Genomics, Inc. Verknüpfte zielerfassung
US11860123B2 (en) 2019-04-16 2024-01-02 The Board Of Trustees Of The University Of Illinois Classification of epigenetic biomarkers and/or DNA conformational superstructures via use of atomically thin nanopores
GB201917060D0 (en) * 2019-11-22 2020-01-08 Oxford Nanopore Tech Ltd Method
WO2021178467A1 (en) 2020-03-03 2021-09-10 Omniome, Inc. Methods and compositions for sequencing double stranded nucleic acids
WO2021255475A1 (en) 2020-06-18 2021-12-23 Oxford Nanopore Technologies Limited A method of selectively characterising a polynucleotide using a detector
KR20230083269A (ko) 2020-07-30 2023-06-09 캠브리지 에피제네틱스 리미티드 핵산 분석을 위한 조성물 및 방법
US11248265B1 (en) 2020-11-19 2022-02-15 Clear Labs, Inc Systems and processes for distinguishing pathogenic and non-pathogenic sequences from specimens
CN113462764B (zh) * 2021-09-01 2021-12-17 北京齐碳科技有限公司 用于表征双链靶多核苷酸的类发夹衔接子、构建体和方法
CN114134142A (zh) * 2021-11-23 2022-03-04 成都齐碳科技有限公司 衔接体、复合物、单链分子、试剂盒、方法和应用
CN114262735A (zh) * 2021-12-15 2022-04-01 成都齐碳科技有限公司 用于表征多核苷酸的衔接体及其用途
CN114457145B (zh) * 2022-01-29 2023-08-11 成都齐碳科技有限公司 用于表征靶多核苷酸测序的接头、构建体、方法和应用
WO2024091124A1 (en) * 2022-10-28 2024-05-02 Rijksuniversiteit Groningen Nanopore-based analysis of proteins

Family Cites Families (178)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE56026B1 (en) 1982-10-19 1991-03-27 Cetus Corp Cysteine-depleted muteins of biologically active proteins
GB8924338D0 (en) 1989-10-28 1989-12-13 Atomic Energy Authority Uk Electrodes
US5215899A (en) 1989-11-09 1993-06-01 Miles Inc. Nucleic acid amplification employing ligatable hairpin probe and transcription
US5424413A (en) 1992-01-22 1995-06-13 Gen-Probe Incorporated Branched nucleic acid probes
FR2703693B1 (fr) 1993-04-06 1995-07-13 Pasteur Institut Procédé rapide de détermination d'une séquence d'ADN et application au séquençage et au diagnostic.
CA2160909A1 (en) 1993-04-28 1994-11-10 Hagan Bayley Cell-targeted lytic pore-forming agents
US5777078A (en) 1993-04-28 1998-07-07 Worcester Foundation For Experimental Biology Triggered pore-forming agents
DE4320201A1 (de) 1993-06-18 1995-01-12 Asta Medica Ag Verwendung von Cetrorelix und weiteren Nona- und Dekapeptiden zur Herstellung eines Arzneimittels zur Bekämpfung von Aids und zur Wachstumsstimulation
US7569341B2 (en) 1994-01-31 2009-08-04 Trustees Of Boston University Nucleic acid directed immobilization arrays and methods of assembly
US5561043A (en) 1994-01-31 1996-10-01 Trustees Of Boston University Self-assembling multimeric nucleic acid constructs
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US6395887B1 (en) 1995-08-01 2002-05-28 Yale University Analysis of gene expression by display of 3'-end fragments of CDNAS
US5866336A (en) 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
WO1999005167A1 (en) 1997-07-25 1999-02-04 University Of Massachusetts Designed protein pores as components for biosensors
US6087099A (en) 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US6127166A (en) 1997-11-03 2000-10-03 Bayley; Hagan Molluscan ligament polypeptides and genes encoding them
JPH11137260A (ja) 1997-11-06 1999-05-25 Soyaku Gijutsu Kenkyusho:Kk 抗インフルエンザウイルス環状ダンベル型rna−dnaキメラ化合物及び抗インフルエンザウイルス剤
US6123819A (en) 1997-11-12 2000-09-26 Protiveris, Inc. Nanoelectrode arrays
DE19826758C1 (de) 1998-06-15 1999-10-21 Soft Gene Gmbh Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6787308B2 (en) * 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6235502B1 (en) 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
US6465193B2 (en) 1998-12-11 2002-10-15 The Regents Of The University Of California Targeted molecular bar codes and methods for using the same
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
AU5763000A (en) * 1999-06-22 2001-01-09 President And Fellows Of Harvard College Molecular and atomic scale evaluation of biopolymers
US7087729B1 (en) 1999-06-29 2006-08-08 University Health Network Peptide conjugates for the stabilization of membrane proteins and interactions with biological membranes
EP1206577B1 (de) 1999-08-13 2006-03-01 Yale University Binär kodierte sequenzmarker
US6682649B1 (en) 1999-10-01 2004-01-27 Sophion Bioscience A/S Substrate and a method for determining and/or monitoring electrophysiological properties of ion channels
EP1238105A2 (de) 1999-12-02 2002-09-11 Molecular Staging Inc. Herstellung einzelsträngiger zirkulärer dna, ausgehend von linearen selbst-hybridisierenden segmenten
EP1255772A2 (de) 2000-02-11 2002-11-13 The Texas A & M University System Zusammensetzungen für biosensoren und deren verwendung
CA2403804A1 (en) 2000-03-21 2001-09-27 Curagen Corporation Vegf-modulated genes and methods employing them
ES2298226T3 (es) 2000-03-22 2008-05-16 Curagen Corporation Polipeptidos relacionados con wnt-1, y acidos nucleicos que los codifican.
US6596488B2 (en) 2000-03-30 2003-07-22 City Of Hope Tumor suppressor gene
US6387624B1 (en) 2000-04-14 2002-05-14 Incyte Pharmaceuticals, Inc. Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US20020132350A1 (en) 2000-09-14 2002-09-19 Pioneer Hi-Bred International, Inc. Targeted genetic manipulation using Mu bacteriophage cleaved donor complex
AU2002239284A1 (en) 2000-11-27 2002-06-03 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
US20030087232A1 (en) 2001-01-25 2003-05-08 Fred Christians Methods for screening polypeptides
US7807408B2 (en) 2001-03-19 2010-10-05 President & Fellows Of Harvard College Directed evolution of proteins
US6863833B1 (en) 2001-06-29 2005-03-08 The Board Of Trustees Of The Leland Stanford Junior University Microfabricated apertures for supporting bilayer lipid membranes
WO2003004992A2 (en) 2001-07-03 2003-01-16 The Regents Of The University Of California Mammalian sweet and amino acid heterodimeric taste receptors
US6852492B2 (en) 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
WO2003078625A2 (en) 2002-03-15 2003-09-25 Nuevolution A/S An improved method for synthesising templated molecules
WO2003095669A1 (en) 2002-05-10 2003-11-20 The Texas A & M University System Stochastic sensing through covalent interactions
WO2004065582A2 (en) 2003-01-15 2004-08-05 Dana-Farber Cancer Institute, Inc. Amplification of dna in a hairpin structure, and applications
CA2515938A1 (en) 2003-02-12 2004-08-26 Genizon Svenska Ab Methods and means for nucleic acid sequencing
WO2004092331A2 (en) 2003-04-08 2004-10-28 Li-Cor, Inc. Composition and method for nucleic acid sequencing
US7163658B2 (en) 2003-04-23 2007-01-16 Rouvain Bension Rapid sequencing of polymers
US7344882B2 (en) 2003-05-12 2008-03-18 Bristol-Myers Squibb Company Polynucleotides encoding variants of the TRP channel family member, LTRPC3
WO2005056750A2 (en) 2003-12-11 2005-06-23 Quark Biotech, Inc. Inversion-duplication of nucleic acids and libraries prepared thereby
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
GB2413796B (en) 2004-03-25 2006-03-29 Global Genomics Ab Methods and means for nucleic acid sequencing
US20050227239A1 (en) * 2004-04-08 2005-10-13 Joyce Timothy H Microarray based affinity purification and analysis device coupled with solid state nanopore electrodes
US7618778B2 (en) * 2004-06-02 2009-11-17 Kaufman Joseph C Producing, cataloging and classifying sequence tags
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
US7700281B2 (en) 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
CA2577079C (en) * 2004-08-13 2014-05-20 President And Fellows Of Harvard College An ultra high-throughput opti-nanopore dna readout platform
US20060086626A1 (en) * 2004-10-22 2006-04-27 Joyce Timothy H Nanostructure resonant tunneling with a gate voltage source
EP1842061A4 (de) 2004-12-21 2009-05-13 Texas A & M Univ Sys Hochtemperatur-ionenkanäle und ionenporen
US7890268B2 (en) 2004-12-28 2011-02-15 Roche Molecular Systems, Inc. De-novo sequencing of nucleic acids
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US7507575B2 (en) 2005-04-01 2009-03-24 3M Innovative Properties Company Multiplex fluorescence detection device having removable optical modules
US7601499B2 (en) * 2005-06-06 2009-10-13 454 Life Sciences Corporation Paired end sequencing
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
US20070031857A1 (en) 2005-08-02 2007-02-08 Rubicon Genomics, Inc. Compositions and methods for processing and amplification of DNA, including using multiple enzymes in a single reaction
WO2007024997A2 (en) 2005-08-22 2007-03-01 Fermalogic, Inc. Methods of increasing production of secondary metabolites
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
US8936926B2 (en) 2005-12-22 2015-01-20 Pacific Biosciences Of California Active surface coupled polymerases
US7932029B1 (en) 2006-01-04 2011-04-26 Si Lok Methods for nucleic acid mapping and identification of fine-structural-variations in nucleic acids and utilities
CA2642854A1 (en) * 2006-02-18 2007-08-30 Michael Strathmann Massively multiplexed sequencing
US8673567B2 (en) * 2006-03-08 2014-03-18 Atila Biosystems, Inc. Method and kit for nucleic acid sequence detection
ES2545264T3 (es) 2006-04-04 2015-09-09 Keygene N.V. Detección de alto rendimiento de marcadores moleculares basada en fragmentos de restricción
WO2007146158A1 (en) 2006-06-07 2007-12-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by nanopore using modified nucleotides
JP4876766B2 (ja) 2006-08-10 2012-02-15 トヨタ自動車株式会社 燃料電池
CA2663047A1 (en) 2006-09-06 2008-03-13 Phase Bioscience, Inc. Therapeutic elastin-like polypeptide (elp) fusion proteins
WO2008045575A2 (en) 2006-10-13 2008-04-17 J. Craig Venter Institute, Inc. Sequencing method
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
GB2445016B (en) 2006-12-19 2012-03-07 Microsaic Systems Plc Microengineered ionisation device
EP2122344B8 (de) 2007-02-20 2019-08-21 Oxford Nanopore Technologies Limited Lipiddoppelschicht-sensorsystem
AU2008236694B2 (en) 2007-04-04 2014-01-23 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
EP2195648B1 (de) 2007-09-12 2019-05-08 President and Fellows of Harvard College Hochauflösender molekularer graphen-sensor mit einer öffnung in der graphenschicht
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
KR101414713B1 (ko) 2007-10-11 2014-07-03 삼성전자주식회사 리가제 및 엔도뉴클레아제의 존재하에서 롤링서클 증폭에의하여 표적 핵산을 증폭하는 방법
CN100554946C (zh) * 2007-12-18 2009-10-28 中国农业大学 一种双链核酸精确定量的方法
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
WO2009084721A1 (en) 2007-12-31 2009-07-09 Fujirebio Inc. Clusters of microresonators for cavity mode optical sensing
US8852864B2 (en) * 2008-01-17 2014-10-07 Sequenom Inc. Methods and compositions for the analysis of nucleic acids
US8231969B2 (en) 2008-03-26 2012-07-31 University Of Utah Research Foundation Asymmetrically functionalized nanoparticles
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2009120374A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
US8143030B2 (en) 2008-09-24 2012-03-27 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
CN104862383B (zh) 2008-03-28 2019-05-28 加利福尼亚太平洋生物科学股份有限公司 用于核酸测序的组合物和方法
JP2012500620A (ja) 2008-04-24 2012-01-12 ザ トラスティーズ オブ コロンビア ユニバーシティー イン ザ シティー オブ ニューヨーク Dna分子組織化のための幾何学的パターンおよび脂質二重層、ならびにその使用
WO2009132315A1 (en) * 2008-04-24 2009-10-29 Life Technologies Corporation Method of sequencing and mapping target nucleic acids
WO2010004265A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Enzyme-pore constructs
CN102144037A (zh) 2008-07-07 2011-08-03 牛津纳米孔技术有限公司 检测碱基的孔
US20100092960A1 (en) 2008-07-25 2010-04-15 Pacific Biosciences Of California, Inc. Helicase-assisted sequencing with molecular beacons
EP2334802A4 (de) * 2008-09-09 2012-01-25 Life Technologies Corp Verfahren zur erstellung genspezifischer bibliotheken
CN102216783B (zh) 2008-09-22 2015-04-01 华盛顿大学 Msp纳米微孔和相关方法
US8383369B2 (en) 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
ES2403756T3 (es) 2008-10-24 2013-05-21 Epicentre Technologies Corporation Composiciones de extremo del transposón y métodos para modificar ácidos nucleicos
US8486630B2 (en) * 2008-11-07 2013-07-16 Industrial Technology Research Institute Methods for accurate sequence data and modified base position determination
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
CN102317473A (zh) * 2008-12-11 2012-01-11 加利福尼亚太平洋生物科学股份有限公司 核酸模板的分类
WO2010086603A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Enzyme mutant
CN102369298B (zh) * 2009-01-30 2017-03-22 牛津纳米孔技术有限公司 跨膜测序中用于核酸构建体的衔接体
DK2396430T3 (da) 2009-02-16 2013-07-15 Epict Technologies Corp Template-uafhængig ligering af enkelt-strenget dna
EP2398494A4 (de) 2009-02-23 2015-10-28 Cytomx Therapeutics Inc Proproteine und anwendungsverfahren dafür
FR2943656A1 (fr) 2009-03-25 2010-10-01 Air Liquide Procede et installation de production d'hydrogene mettant en oeuvre un compresseur thermocinetique
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
US8986928B2 (en) * 2009-04-10 2015-03-24 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
DK2422198T3 (da) 2009-04-20 2014-01-06 Oxford Nanopore Tech Ltd Lipiddobbeltlag-sensorgruppe
JP5873023B2 (ja) 2009-12-01 2016-03-01 オックスフォード ナノポール テクノロジーズ リミテッド 生化学分析機器
WO2011090556A1 (en) * 2010-01-19 2011-07-28 Verinata Health, Inc. Methods for determining fraction of fetal nucleic acid in maternal samples
FR2955773B1 (fr) 2010-02-01 2017-05-26 Commissariat A L'energie Atomique Complexe moleculaire de ciblage des antigenes vers les cellules presentatrices d'antigene et ses applications pour la vaccination
KR20110100963A (ko) * 2010-03-05 2011-09-15 삼성전자주식회사 미세 유동 장치 및 이를 이용한 표적 핵산의 염기 서열 결정 방법
WO2011112718A1 (en) 2010-03-10 2011-09-15 Ibis Biosciences, Inc. Production of single-stranded circular nucleic acid
US8652779B2 (en) 2010-04-09 2014-02-18 Pacific Biosciences Of California, Inc. Nanopore sequencing using charge blockade labels
US20120244525A1 (en) 2010-07-19 2012-09-27 New England Biolabs, Inc. Oligonucleotide Adapters: Compositions and Methods of Use
CN103392008B (zh) 2010-09-07 2017-10-20 加利福尼亚大学董事会 通过持续性酶以一个核苷酸的精度控制dna在纳米孔中的移动
AU2011323107B2 (en) 2010-11-05 2015-09-10 Illumina, Inc. Linking sequence reads using paired code tags
ES2641871T3 (es) 2010-12-17 2017-11-14 The Trustees Of Columbia University In The City Of New York Secuenciación de ADN mediante síntesis usando nucleótidos modificados y detección con nanoporos
WO2012098561A2 (en) 2011-01-18 2012-07-26 Swamy R K A multipupurpose instrument for triangle solutions, measurements and geometrical applications called triometer
WO2012098562A2 (en) 2011-01-19 2012-07-26 Panacea Biotec Limited Liquid oral compositions of lanthanum salts
CA2821559C (en) 2011-01-28 2017-01-31 Illumina, Inc. Oligonucleotide replacement for di-tagged and directional libraries
US20120196279A1 (en) 2011-02-02 2012-08-02 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
US9751915B2 (en) 2011-02-11 2017-09-05 Oxford Nanopore Technologies Ltd. Mutant pores
AU2012264497B2 (en) 2011-05-27 2017-06-15 Oxford Nanopore Technologies Limited Coupling method
US20130017978A1 (en) 2011-07-11 2013-01-17 Finnzymes Oy Methods and transposon nucleic acids for generating a dna library
US9145623B2 (en) 2011-07-20 2015-09-29 Thermo Fisher Scientific Oy Transposon nucleic acids comprising a calibration sequence for DNA sequencing
IN2014DN00221A (de) 2011-07-25 2015-06-05 Oxford Nanopore Tech Ltd
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US20160162634A1 (en) 2011-09-23 2016-06-09 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
US20140308661A1 (en) 2011-09-25 2014-10-16 Theranos, Inc. Systems and methods for multi-analysis
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
EP2987870B1 (de) 2011-10-21 2020-02-19 Oxford Nanopore Technologies Limited Verfahren zur kennzeichnung eines zielpolynukleotids mit einer transmembranpore und eines molekularen motors
CN104136631B (zh) 2011-12-29 2017-03-01 牛津纳米孔技术公司 使用xpd解旋酶表征多核苷酸的方法
WO2013098562A2 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Enzyme method
NO2694769T3 (de) 2012-03-06 2018-03-03
EP2836506B1 (de) 2012-04-10 2017-04-19 Oxford Nanopore Technologies Limited Lysenin mutant poren
WO2013185137A1 (en) 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
TWI655213B (zh) 2012-07-13 2019-04-01 目立康股份有限公司 自我組織化肽衍生物的製造方法
EP2875154B1 (de) 2012-07-19 2017-08-23 Oxford Nanopore Technologies Limited SSB-Verfahren zur Charakterisierung einer Nukleinsäure
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
EP2875128B8 (de) 2012-07-19 2020-06-24 Oxford Nanopore Technologies Limited Modifizierte helikasen
US9551023B2 (en) 2012-09-14 2017-01-24 Oxford Nanopore Technologies Ltd. Sample preparation method
CN104936682B (zh) 2012-10-26 2017-12-15 牛津纳米孔技术公司 微滴界面
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
WO2014074922A1 (en) 2012-11-09 2014-05-15 Stratos Genomics, Inc. Concentrating a target molecule for sensing by a nanopore
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US20140206842A1 (en) 2013-01-22 2014-07-24 Muhammed Majeed Peptides Modified with Triterpenoids and Small Organic Molecules: Synthesis and use in Cosmeceutical
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
EP2964779B1 (de) 2013-03-08 2018-08-29 Oxford Nanopore Technologies Limited Verwendung von spacer-elementen in einer nukleinsäure zur kontrolle der bewegung einer helicase
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
AU2013382098B2 (en) 2013-03-13 2019-02-07 Illumina, Inc. Methods and compositions for nucleic acid sequencing
EP2976435B1 (de) 2013-03-19 2017-10-25 Directed Genomics, LLC Anreicherung von zielsequenzen
CN105992634B (zh) 2013-08-30 2019-06-14 华盛顿大学商业中心 选择性修饰聚合物亚单位以改进基于纳米孔的分析
CN105899678A (zh) 2013-10-18 2016-08-24 牛津纳米孔技术公司 经修饰的酶
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
WO2015110813A1 (en) 2014-01-22 2015-07-30 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US10131944B2 (en) 2014-03-24 2018-11-20 The Regents Of The University Of California Molecular adapter for capture and manipulation of transfer RNA
EP3155125A1 (de) 2014-06-13 2017-04-19 Illumina Cambridge Limited Verfahren und zusammensetzungen zur herstellung von sequenzierungsbibliotheken
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
AU2015284464B2 (en) 2014-06-30 2021-06-17 Illumina, Inc. Methods and compositions using one-sided transposition
WO2016022557A1 (en) 2014-08-05 2016-02-11 Twist Bioscience Corporation Cell free cloning of nucleic acids
EP3633047B1 (de) 2014-08-19 2022-12-28 Pacific Biosciences of California, Inc. Verfahren zur sequenzierung von nukleinsäuren basierend auf einer anreicherung von nukleinsäuren
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
DE112016000293T5 (de) 2015-02-26 2017-09-21 Hitachi High-Technologies Corporation Verfahren zum konstruieren eines nucleinsäure-moleküls
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN107488656B (zh) 2016-06-13 2020-07-17 陆欣华 一种核酸等温自扩增方法
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method

Also Published As

Publication number Publication date
US10851409B2 (en) 2020-12-01
JP2014531196A (ja) 2014-11-27
EP2737084A1 (de) 2014-06-04
US10597713B2 (en) 2020-03-24
US20180291441A1 (en) 2018-10-11
US20160281159A1 (en) 2016-09-29
AU2012288629B2 (en) 2017-02-02
US20150152492A1 (en) 2015-06-04
US20160362739A1 (en) 2016-12-15
KR20140050067A (ko) 2014-04-28
AU2012288629A1 (en) 2014-03-06
US20220127669A1 (en) 2022-04-28
CN103827320A (zh) 2014-05-28
US11168363B2 (en) 2021-11-09
CA2843136C (en) 2020-12-29
US9957560B2 (en) 2018-05-01
WO2013014451A1 (en) 2013-01-31
EP2737084B1 (de) 2017-10-18
US11261487B2 (en) 2022-03-01
US20200024655A1 (en) 2020-01-23
JP6298404B2 (ja) 2018-03-20
CN103827320B (zh) 2017-04-26
US20200239950A1 (en) 2020-07-30
CA2843136A1 (en) 2013-01-31
BR112014001699A2 (pt) 2017-06-13

Similar Documents

Publication Publication Date Title
IN2014DN00221A (de)
HRP20181357T1 (hr) Novi postupci pročišćavanja proteina
HK1214277A1 (zh) 生產異源多聚體蛋白質
ZA201207025B (en) Selective extraction of proteins from freshwater algae
WO2013151668A3 (en) Modified polynucleotides for the production of secreted proteins
WO2011156529A3 (en) Methods and composition for multiplex sequencing
BR112014000352A2 (pt) método de purificar uma proteína de fusão-fc, proteínas de fusão contendo fc purificada, tnfr:fc purificada, e tnfr:fc elevadamente purificada
HK1206072A1 (zh) 核酸測序的改進方法
HK1211981A1 (en) Methods to control protein heterogeneity
HK1200869A1 (en) Nucleic acid fragment binding to target protein
IN2015MN00139A (de)
NZ706909A (en) Novel α4ß7 peptide dimer antagonists
WO2012109373A3 (en) Treatment of osteoarthritis and pain
GB201220140D0 (en) Use of Nanopore arrays for multiplex sequencing of nucleic acids
HK1183911A1 (en) Method of dna sequencing by hybridisation dna
MX2013013832A (es) Proteínas de unión receptoras fc.
HK1183912A1 (en) Method of dna sequencing by polymerisation dna
IL230854A (en) Fluorine compounds associated with glycoprotein-specific blood platelets iiia / iib, methods of manufacture and use thereof
DK2598516T3 (da) Fremgangsmåde til proteinoprensning
MY162901A (en) Surface-treated calcium carbonate for binding and bioremediating hydrocarbon-containing compositions
WO2013181404A3 (en) COMPOSITIONS AND METHODS FOR PEPTIDE EXPRESSION AND PURIFICATION USING A TYPE lll SECRETION SYSTEM
EA201590845A1 (ru) Варианты fc-гамма-рецептора iib
WO2012136898A3 (en) Novel cutinases, their production and uses
IL228491A0 (en) Methods for the purification of viruses using gel permeation chromatography
EP3265589A4 (de) Polynukleotid-bindende proteinsequenzierung