IL89847A - פירוק מיקרובי של טריכלורואתילן - Google Patents
פירוק מיקרובי של טריכלורואתילןInfo
- Publication number
- IL89847A IL89847A IL8984789A IL8984789A IL89847A IL 89847 A IL89847 A IL 89847A IL 8984789 A IL8984789 A IL 8984789A IL 8984789 A IL8984789 A IL 8984789A IL 89847 A IL89847 A IL 89847A
- Authority
- IL
- Israel
- Prior art keywords
- tce
- cells
- toluene
- trichloroethylene
- degradation
- Prior art date
Links
- XSTXAVWGXDQKEL-UHFFFAOYSA-N Trichloroethylene Chemical group ClC=C(Cl)Cl XSTXAVWGXDQKEL-UHFFFAOYSA-N 0.000 title claims description 232
- 238000006731 degradation reaction Methods 0.000 title claims description 86
- 230000015556 catabolic process Effects 0.000 title claims description 85
- UBOXGVDOUJQMTN-UHFFFAOYSA-N trichloroethylene Natural products ClCC(Cl)Cl UBOXGVDOUJQMTN-UHFFFAOYSA-N 0.000 title claims description 19
- 230000000813 microbial effect Effects 0.000 title claims description 11
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 claims description 357
- 244000005700 microbiome Species 0.000 claims description 83
- 238000000034 method Methods 0.000 claims description 56
- 108010074633 Mixed Function Oxygenases Proteins 0.000 claims description 44
- 239000013612 plasmid Substances 0.000 claims description 44
- 239000000411 inducer Substances 0.000 claims description 26
- 230000000593 degrading effect Effects 0.000 claims description 22
- 239000002894 chemical waste Substances 0.000 claims description 12
- 241000589755 Pseudomonas mendocina Species 0.000 claims description 10
- 238000011109 contamination Methods 0.000 claims description 9
- 239000003673 groundwater Substances 0.000 claims description 8
- 239000002689 soil Substances 0.000 claims description 7
- 241000589776 Pseudomonas putida Species 0.000 claims description 3
- 239000003651 drinking water Substances 0.000 claims description 3
- 235000020188 drinking water Nutrition 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 claims description 3
- 239000002699 waste material Substances 0.000 claims description 2
- 125000003944 tolyl group Chemical group 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 description 49
- 102000004190 Enzymes Human genes 0.000 description 39
- 108090000790 Enzymes Proteins 0.000 description 39
- 238000003556 assay Methods 0.000 description 27
- 239000002609 medium Substances 0.000 description 27
- 230000012010 growth Effects 0.000 description 25
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 21
- 230000000694 effects Effects 0.000 description 21
- 239000000463 material Substances 0.000 description 21
- 239000000047 product Substances 0.000 description 20
- 230000004060 metabolic process Effects 0.000 description 19
- 230000003413 degradative effect Effects 0.000 description 18
- 102000004169 proteins and genes Human genes 0.000 description 18
- 102000008109 Mixed Function Oxygenases Human genes 0.000 description 17
- 230000037361 pathway Effects 0.000 description 16
- 239000000356 contaminant Substances 0.000 description 14
- 229930195733 hydrocarbon Natural products 0.000 description 13
- 150000002430 hydrocarbons Chemical class 0.000 description 13
- 239000000523 sample Substances 0.000 description 13
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 241000588724 Escherichia coli Species 0.000 description 10
- YNQLUTRBYVCPMQ-UHFFFAOYSA-N Ethylbenzene Chemical compound CCC1=CC=CC=C1 YNQLUTRBYVCPMQ-UHFFFAOYSA-N 0.000 description 10
- 230000001580 bacterial effect Effects 0.000 description 10
- 239000006285 cell suspension Substances 0.000 description 10
- 230000006698 induction Effects 0.000 description 10
- 239000000126 substance Substances 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 9
- 230000001965 increasing effect Effects 0.000 description 9
- 239000004215 Carbon black (E152) Substances 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 8
- 239000008103 glucose Substances 0.000 description 8
- 150000002500 ions Chemical class 0.000 description 8
- 150000002894 organic compounds Chemical class 0.000 description 8
- IWDCLRJOBJJRNH-UHFFFAOYSA-N p-cresol Chemical compound CC1=CC=C(O)C=C1 IWDCLRJOBJJRNH-UHFFFAOYSA-N 0.000 description 8
- 238000004113 cell culture Methods 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- -1 chlorinated aromatic organic compounds Chemical class 0.000 description 7
- 238000011161 development Methods 0.000 description 7
- 230000008034 disappearance Effects 0.000 description 7
- 239000007789 gas Substances 0.000 description 7
- 238000011534 incubation Methods 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 239000013598 vector Substances 0.000 description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 6
- 238000013459 approach Methods 0.000 description 6
- 229910052799 carbon Inorganic materials 0.000 description 6
- JXTHNDFMNIQAHM-UHFFFAOYSA-N dichloroacetic acid Chemical compound OC(=O)C(Cl)Cl JXTHNDFMNIQAHM-UHFFFAOYSA-N 0.000 description 6
- 238000004817 gas chromatography Methods 0.000 description 6
- 239000000758 substrate Substances 0.000 description 6
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 5
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 5
- 125000001931 aliphatic group Chemical group 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 5
- 230000001939 inductive effect Effects 0.000 description 5
- 230000002503 metabolic effect Effects 0.000 description 5
- 239000013600 plasmid vector Substances 0.000 description 5
- 239000010891 toxic waste Substances 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- HHLFWLYXYJOTON-UHFFFAOYSA-N glyoxylic acid Chemical compound OC(=O)C=O HHLFWLYXYJOTON-UHFFFAOYSA-N 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 239000002207 metabolite Substances 0.000 description 4
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 4
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 4
- 239000012071 phase Substances 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 231100000331 toxic Toxicity 0.000 description 4
- 230000002588 toxic effect Effects 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 3
- 229960005215 dichloroacetic acid Drugs 0.000 description 3
- 230000008030 elimination Effects 0.000 description 3
- 238000003379 elimination reaction Methods 0.000 description 3
- 238000010828 elution Methods 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 230000002906 microbiologic effect Effects 0.000 description 3
- 231100000719 pollutant Toxicity 0.000 description 3
- 229910000160 potassium phosphate Inorganic materials 0.000 description 3
- 235000011009 potassium phosphates Nutrition 0.000 description 3
- 238000002731 protein assay Methods 0.000 description 3
- 230000002285 radioactive effect Effects 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 239000003643 water by type Substances 0.000 description 3
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 2
- 229930195714 L-glutamate Natural products 0.000 description 2
- 101100446506 Mus musculus Fgf3 gene Proteins 0.000 description 2
- UFWIBTONFRDIAS-UHFFFAOYSA-N Naphthalene Chemical compound C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 description 2
- 241000219492 Quercus Species 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 239000008346 aqueous phase Substances 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 239000007857 degradation product Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 235000019253 formic acid Nutrition 0.000 description 2
- 239000000383 hazardous chemical Substances 0.000 description 2
- 239000004434 industrial solvent Substances 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 229930027917 kanamycin Natural products 0.000 description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
- 229960000318 kanamycin Drugs 0.000 description 2
- 229930182823 kanamycin A Natural products 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- QWVGKYWNOKOFNN-UHFFFAOYSA-N o-cresol Chemical compound CC1=CC=CC=C1O QWVGKYWNOKOFNN-UHFFFAOYSA-N 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 230000002085 persistent effect Effects 0.000 description 2
- 230000027086 plasmid maintenance Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000036647 reaction Effects 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000004809 thin layer chromatography Methods 0.000 description 2
- 231100000167 toxic agent Toxicity 0.000 description 2
- QFLWZFQWSBQYPS-AWRAUJHKSA-N (3S)-3-[[(2S)-2-[[(2S)-2-[5-[(3aS,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]-3-methylbutanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-4-[1-bis(4-chlorophenoxy)phosphorylbutylamino]-4-oxobutanoic acid Chemical compound CCCC(NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H](NC(=O)CCCCC1SC[C@@H]2NC(=O)N[C@H]12)C(C)C)P(=O)(Oc1ccc(Cl)cc1)Oc1ccc(Cl)cc1 QFLWZFQWSBQYPS-AWRAUJHKSA-N 0.000 description 1
- WSLDOOZREJYCGB-UHFFFAOYSA-N 1,2-Dichloroethane Chemical compound ClCCCl WSLDOOZREJYCGB-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000237074 Centris Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 244000309719 Escherichia coli F5 Species 0.000 description 1
- 241000588748 Klebsiella Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000004020 Oxygenases Human genes 0.000 description 1
- 108090000417 Oxygenases Proteins 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 108010075474 Toluene dioxygenase Proteins 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- BZHJMEDXRYGGRV-UHFFFAOYSA-N Vinyl chloride Chemical compound ClC=C BZHJMEDXRYGGRV-UHFFFAOYSA-N 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 150000001491 aromatic compounds Chemical class 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
- WDIHJSXYQDMJHN-UHFFFAOYSA-L barium chloride Chemical compound [Cl-].[Cl-].[Ba+2] WDIHJSXYQDMJHN-UHFFFAOYSA-L 0.000 description 1
- 229910001626 barium chloride Inorganic materials 0.000 description 1
- CCDWGDHTPAJHOA-UHFFFAOYSA-N benzylsilicon Chemical compound [Si]CC1=CC=CC=C1 CCDWGDHTPAJHOA-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000012159 carrier gas Substances 0.000 description 1
- 230000006652 catabolic pathway Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000149 chemical water pollutant Substances 0.000 description 1
- FOCAUTSVDIKZOP-UHFFFAOYSA-N chloroacetic acid Chemical compound OC(=O)CCl FOCAUTSVDIKZOP-UHFFFAOYSA-N 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000006957 competitive inhibition Effects 0.000 description 1
- 230000001268 conjugating effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 238000006298 dechlorination reaction Methods 0.000 description 1
- 238000005202 decontamination Methods 0.000 description 1
- 230000003588 decontaminative effect Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 230000005264 electron capture Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000003517 fume Substances 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 229930195712 glutamate Natural products 0.000 description 1
- 239000002920 hazardous waste Substances 0.000 description 1
- 239000001307 helium Substances 0.000 description 1
- 229910052734 helium Inorganic materials 0.000 description 1
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
- 239000003295 industrial effluent Substances 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical compound C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 description 1
- 230000007269 microbial metabolism Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 150000002896 organic halogen compounds Chemical class 0.000 description 1
- PVSCLHDHWOAWGV-UHFFFAOYSA-N oxygen;toluene Chemical compound [O].CC1=CC=CC=C1 PVSCLHDHWOAWGV-UHFFFAOYSA-N 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 229920001921 poly-methyl-phenyl-siloxane Polymers 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/38—Pseudomonas
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/874—Pseudomonas
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17764088A | 1988-04-05 | 1988-04-05 | |
| US07/235,354 US5079166A (en) | 1988-04-05 | 1988-10-19 | Microbial degradation of trichloroethylene |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL89847A0 IL89847A0 (en) | 1989-12-15 |
| IL89847A true IL89847A (he) | 1994-10-21 |
Family
ID=26873499
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL8984789A IL89847A (he) | 1988-04-05 | 1989-04-04 | פירוק מיקרובי של טריכלורואתילן |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US5079166A (he) |
| EP (1) | EP0336718A3 (he) |
| JP (1) | JPH02503866A (he) |
| KR (1) | KR0131772B1 (he) |
| CA (1) | CA1316860C (he) |
| DK (1) | DK175623B1 (he) |
| FI (1) | FI100992B (he) |
| IL (1) | IL89847A (he) |
| NO (1) | NO179642C (he) |
| PT (1) | PT90196B (he) |
| WO (1) | WO1989009827A1 (he) |
Families Citing this family (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4009109A1 (de) * | 1990-03-21 | 1991-09-26 | Fraunhofer Ges Forschung | Verfahren zur mikrobiologischen reinigung von mit halogenierten ethenen und/oder mit halogenierten butadienen kontaminierten abluftstroemen |
| US5487834A (en) * | 1993-02-09 | 1996-01-30 | The Regents Of The University Of California | Methods for microbial filtration of fluids |
| US5397473A (en) * | 1993-08-27 | 1995-03-14 | Cornell Research Foundation, Inc. | Biological treatment method for water |
| US5512479A (en) * | 1993-10-14 | 1996-04-30 | Envirogen, Inc. | Method of degrading volatile organochlorides and remediation thereof |
| US5612204A (en) * | 1994-07-08 | 1997-03-18 | Japan Energy Corporation | Biological degradative treatment of chlorine substituted ethylene |
| US5633164A (en) * | 1994-12-16 | 1997-05-27 | Cytec Technology Corporaton | Methods for fluid phase biodegradation |
| HUT78053A (hu) * | 1994-12-16 | 1999-07-28 | Cytec Technology Corp. | Biodegradáló rendszerek és módszerek |
| US5585272A (en) * | 1994-12-16 | 1996-12-17 | Cytec Technology Corporation | Solid phase system for aerobic degradation |
| US5688685A (en) * | 1994-12-16 | 1997-11-18 | Cytec Technology Corporation | System and methods for biodegradation of compounds |
| US5641679A (en) * | 1994-12-16 | 1997-06-24 | Cytec Technology Corporation | Methods for bio-remediation |
| JP3108006B2 (ja) * | 1995-02-28 | 2000-11-13 | キヤノン株式会社 | Ｊｍ１株、環境修復方法、有機化合物の分解方法、微生物の取得方法、微生物の検出方法、及び微生物の検出キット |
| US5688660A (en) * | 1995-03-15 | 1997-11-18 | Colgate-Palmolive Company | Method for determining product biodegradability |
| US5747328A (en) * | 1996-02-28 | 1998-05-05 | Wisconsin Alumni Research Foundation | Microbial system for formaldehyde sensing and remediation |
| US6242244B1 (en) | 1996-02-28 | 2001-06-05 | Wisconsin Alumni Research Foundation | Microbial system for formaldehyde sensing and remediation |
| JP3083077B2 (ja) * | 1996-04-11 | 2000-09-04 | キヤノン株式会社 | 有機化合物の生分解方法及び環境修復方法 |
| JP3323746B2 (ja) * | 1996-08-01 | 2002-09-09 | キヤノン株式会社 | 新規微生物、有機化合物の生分解方法及び環境修復方法 |
| JP3347596B2 (ja) * | 1996-08-01 | 2002-11-20 | キヤノン株式会社 | 新規微生物、芳香族化合物及び／或いは有機塩素化合物の生分解方法、及び媒体の浄化方法 |
| US6171844B1 (en) | 1996-08-19 | 2001-01-09 | Toyota Jidosha Kabushiki Kaisha | Microorganism and method for environmental purification using the same |
| US5958757A (en) * | 1996-09-13 | 1999-09-28 | Envirogen, Inc. | Biological conversion of organic compounds |
| US6218172B1 (en) | 1996-10-07 | 2001-04-17 | Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. | Microbiological method for eliminating halogenated ethenes |
| DE19709453C2 (de) * | 1996-10-07 | 2000-07-13 | Fraunhofer Ges Forschung | Verwendung von Bakterienstämmen die aus Proben angereichert wurden, die sowohl Ethen als auch Chlorethen ausgesetzt waren zum Abbau von halogeniertem Ethen |
| DE19710010A1 (de) * | 1997-03-12 | 1998-09-17 | Solvay Deutschland | Verfahren zur biologischen Behandlung von Grund- oder Prozeßabwässern zur Entfernung von halogenierten, ungesättigten Kohlenwasserstoffen |
| US6830899B1 (en) | 1997-06-13 | 2004-12-14 | E. I. Du Pont De Nemours And Company | Method for the production of para-hydroxybenzoate in Pseudomonas mendocina |
| DE19730653A1 (de) * | 1997-07-17 | 1999-01-21 | Forschungszentrum Fuer Medizin | Verfahren zum mikrobiologischen Abbau halogenierter aliphatischer und aromatischer Kohlenwasserstoffe in kontaminierten Medien |
| US5994120A (en) * | 1998-02-13 | 1999-11-30 | Infectech, Inc. | Method of bioremediating an organic hazardous substance |
| US6864074B2 (en) | 1998-10-30 | 2005-03-08 | Canon Kabushiki Kaisha | Dna fragment carrying toluene monooxygenase gene, recombinant plasmid, transformed microorganism, method for degrading chlorinated aliphatic hydrocarbon compounds and aromatic compounds, and method for environmental remediation |
| EP1006191A3 (en) | 1998-12-03 | 2002-02-06 | Canon Kabushiki Kaisha | DNA encoding toluene monooxygenase, method for degrading chlorinated aliphatic hydrocarbons and aromatic compounds and method for environment remediation |
| US6586229B1 (en) | 2000-06-01 | 2003-07-01 | North Carolina State University | Method for the production of ρ-Hydroxybenzoate in species of pseudomonas and agrobacterium |
| JP2004504025A (ja) * | 2000-07-18 | 2004-02-12 | ナショナル・リサーチ・カウンシル・オブ・カナダ | 大腸菌における、コマモナスのシクロペンタノン１，２−モノオキシゲナーゼをコードする遺伝子 |
| AU2003211662A1 (en) * | 2002-02-26 | 2003-09-09 | Junichi Kato | Benzene-utilizing rhodococcus and plasmid prepared thereby |
| TWI435847B (zh) * | 2005-04-21 | 2014-05-01 | Ibiden Co Ltd | An additive for the treatment of waste liquid containing organic substances |
| US8658411B2 (en) * | 2005-04-21 | 2014-02-25 | Ibiden Co., Ltd. | Method of treating wastewater containing organic compound |
| US8277657B2 (en) * | 2008-08-08 | 2012-10-02 | University Of Massachusetts | Systems and methods for microbial reductive dechlorination of environmental contaminants |
| CN108070544B (zh) * | 2017-12-29 | 2021-04-09 | 佛山市碧沃丰生物科技股份有限公司 | 门多萨假单胞菌及其培养基、发酵方法和应用 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4477570A (en) * | 1981-09-24 | 1984-10-16 | Occidental Chemical Corporation | Microbial degradation of obnoxious organic wastes into innocucous materials |
| US4520103A (en) * | 1982-10-27 | 1985-05-28 | Amgen | Microbial production of indigo |
| CA1262339A (en) * | 1984-08-14 | 1989-10-17 | Stanley A. Sojka | Cloning the halo-organic biodegradable gene |
| US4959315A (en) * | 1987-04-30 | 1990-09-25 | The United States Of America As Represented By The Administrator Of The Environmental Protection Agency | Biodegradation of chloroethylene compounds |
-
1988
- 1988-10-19 US US07/235,354 patent/US5079166A/en not_active Expired - Lifetime
-
1989
- 1989-04-03 CA CA000595483A patent/CA1316860C/en not_active Expired - Lifetime
- 1989-04-04 PT PT90196A patent/PT90196B/pt not_active IP Right Cessation
- 1989-04-04 JP JP1504759A patent/JPH02503866A/ja active Pending
- 1989-04-04 WO PCT/US1989/001419 patent/WO1989009827A1/en not_active Ceased
- 1989-04-04 EP EP19890303328 patent/EP0336718A3/en not_active Ceased
- 1989-04-04 KR KR1019890702298A patent/KR0131772B1/ko not_active Expired - Fee Related
- 1989-04-04 IL IL8984789A patent/IL89847A/he unknown
- 1989-12-04 FI FI895787A patent/FI100992B/fi not_active IP Right Cessation
- 1989-12-04 NO NO894846A patent/NO179642C/no not_active IP Right Cessation
- 1989-12-04 DK DK198906089A patent/DK175623B1/da not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| FI895787A0 (fi) | 1989-12-04 |
| NO894846D0 (no) | 1989-12-04 |
| WO1989009827A1 (en) | 1989-10-19 |
| DK608989A (da) | 1990-02-02 |
| NO179642B (no) | 1996-08-12 |
| AU3427589A (en) | 1989-11-03 |
| NO179642C (no) | 1996-11-20 |
| CA1316860C (en) | 1993-04-27 |
| DK608989D0 (da) | 1989-12-04 |
| DK175623B1 (da) | 2004-12-27 |
| EP0336718A3 (en) | 1991-04-24 |
| KR900700611A (ko) | 1990-08-16 |
| KR0131772B1 (ko) | 1998-04-11 |
| EP0336718A2 (en) | 1989-10-11 |
| JPH02503866A (ja) | 1990-11-15 |
| US5079166A (en) | 1992-01-07 |
| PT90196B (pt) | 1994-06-30 |
| NO894846L (no) | 1989-12-04 |
| AU626856B2 (en) | 1992-08-13 |
| FI100992B (fi) | 1998-03-31 |
| PT90196A (pt) | 1989-11-10 |
| IL89847A0 (en) | 1989-12-15 |
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