IL255647B - Preparations and methods for enriching nucleic acid populations - Google Patents

Preparations and methods for enriching nucleic acid populations

Info

Publication number
IL255647B
IL255647B IL255647A IL25564717A IL255647B IL 255647 B IL255647 B IL 255647B IL 255647 A IL255647 A IL 255647A IL 25564717 A IL25564717 A IL 25564717A IL 255647 B IL255647 B IL 255647B
Authority
IL
Israel
Prior art keywords
compositions
methods
nucleic acids
enriching populations
enriching
Prior art date
Application number
IL255647A
Other languages
English (en)
Hebrew (he)
Other versions
IL255647A (en
Original Assignee
Karius Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Karius Inc filed Critical Karius Inc
Publication of IL255647A publication Critical patent/IL255647A/en
Publication of IL255647B publication Critical patent/IL255647B/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/155Modifications characterised by incorporating/generating a new priming site
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/161Modifications characterised by incorporating target specific and non-target specific sites
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/159Reduction of complexity, e.g. amplification of subsets, removing duplicated genomic regions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
IL255647A 2015-05-18 2017-11-14 Preparations and methods for enriching nucleic acid populations IL255647B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562163273P 2015-05-18 2015-05-18
US201662334348P 2016-05-10 2016-05-10
PCT/US2016/032936 WO2016187234A1 (en) 2015-05-18 2016-05-17 Compositions and methods for enriching populations of nucleic acids

Publications (2)

Publication Number Publication Date
IL255647A IL255647A (en) 2018-01-31
IL255647B true IL255647B (en) 2022-04-01

Family

ID=57320699

Family Applications (1)

Application Number Title Priority Date Filing Date
IL255647A IL255647B (en) 2015-05-18 2017-11-14 Preparations and methods for enriching nucleic acid populations

Country Status (10)

Country Link
US (3) US11111520B2 (https=)
EP (2) EP4450636A3 (https=)
JP (3) JP6873921B2 (https=)
CN (1) CN107922971A (https=)
AU (1) AU2016264102A1 (https=)
BR (1) BR112017024747A2 (https=)
CA (1) CA2986036C (https=)
IL (1) IL255647B (https=)
WO (1) WO2016187234A1 (https=)
ZA (1) ZA201708264B (https=)

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016100955A2 (en) 2014-12-20 2016-06-23 Identifygenomics, Llc Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using crispr/cas system proteins
JP6873921B2 (ja) 2015-05-18 2021-05-19 カリウス・インコーポレイテッド 核酸の集団を濃縮するための組成物および方法
US10538758B2 (en) 2015-08-19 2020-01-21 Arc Bio, Llc Capture of nucleic acids using a nucleic acid-guided nuclease-based system
EP3377654B1 (en) 2015-11-16 2024-01-31 Revere Biosensors, LLC Systems and methods for identifying and distinguishing genetic samples
EP3978627A1 (en) 2016-03-25 2022-04-06 Karius, Inc. Methods using synthetic nucleic acid spike-ins
GB2559319B (en) * 2016-12-23 2019-01-16 Cs Genetics Ltd Reagents and methods for the analysis of linked nucleic acids
WO2018119399A1 (en) 2016-12-23 2018-06-28 Grail, Inc. Methods for high efficiency library preparation using double-stranded adapters
WO2018140452A1 (en) * 2017-01-30 2018-08-02 Counsyl, Inc. Enrichment of cell-free dna from a biological sample
JP6637218B2 (ja) * 2017-03-27 2020-01-29 積水メディカル株式会社 抗体を用いた標的核酸濃縮及び回収法
US11274344B2 (en) 2017-03-30 2022-03-15 Grail, Inc. Enhanced ligation in sequencing library preparation
WO2018183897A1 (en) * 2017-03-31 2018-10-04 Grail, Inc. Higher target capture efficiency using probe extension
WO2018191563A1 (en) 2017-04-12 2018-10-18 Karius, Inc. Sample preparation methods, systems and compositions
WO2018195951A1 (en) * 2017-04-28 2018-11-01 Coyote Diagnostics Lab (Beijing) Co., Ltd. Integrated sample collection, processing and analysis systems
CN110869512A (zh) 2017-05-09 2020-03-06 雷瑞生物传感器有限责任公司 鉴别并区分遗传样品的系统及方法
JP2021500077A (ja) 2017-10-18 2021-01-07 デイ ゼロ ダイアグノスティックス, インコーポレイテッド Dna増幅の標的化抑制による混合dna試料中のdna集団の選択的富化
CN108220479B (zh) * 2018-01-05 2021-08-13 浙江农林大学 能检测多种猪猝死症病原的多重连接探针扩增鉴别试剂盒
CN110272984A (zh) * 2018-03-13 2019-09-24 深圳华大生命科学研究院 测序获取寄生虫核酸信息及对寄生虫分类的方法和系统
US20200087709A1 (en) * 2018-03-13 2020-03-19 Microinvestigate, Llc Direct-to-consumer genomic diagnostic device
CA3082601A1 (en) 2018-03-16 2019-09-19 Karius, Inc. Sample series to differentiate target nucleic acids from contaminant nucleic acids
EP3768856B1 (en) 2018-03-19 2025-12-17 Illumina, Inc. Methods and compositions for selective cleavage of nucleic acids with recombinant nucleases
GB201810571D0 (en) * 2018-06-27 2018-08-15 Cs Genetics Ltd Reagents and methods for the analysis of circulating microparticles
EP3815092A2 (en) 2018-06-29 2021-05-05 F. Hoffmann-La Roche AG Detection of microsatellite instability
US20210383924A1 (en) * 2018-10-25 2021-12-09 Quadrant Biosciences Inc. Methods and machine learning for disease diagnosis
EP4700161A3 (en) 2018-11-21 2026-03-11 Karius, Inc. Direct-to-library methods, systems, and compositions
CN121575489A (zh) * 2018-11-21 2026-02-27 卡里乌斯公司 感染性疾病的检测和预测
JP7132100B2 (ja) * 2018-11-21 2022-09-06 株式会社日立製作所 生体分子分析装置及び生体分子分析方法
CN109355365A (zh) * 2018-11-25 2019-02-19 中国农业科学院兰州兽医研究所 一种小rna高通量测序检测微生物的方法
WO2020118543A1 (zh) * 2018-12-12 2020-06-18 深圳华大生命科学研究院 分离和/或富集宿主源核酸和病原核酸的方法和试剂及其制备方法
US20200232010A1 (en) * 2018-12-20 2020-07-23 Guardant Health, Inc. Methods, compositions, and systems for improving recovery of nucleic acid molecules
US12565676B2 (en) 2019-03-01 2026-03-03 Revere Biosensors, Llc Systems and methods for separating decoded arrays
GB201902887D0 (en) * 2019-03-04 2019-04-17 St Georges Hospital Medical School Detection and antibiotic resistance profiling of microorganisms
WO2020198664A1 (en) * 2019-03-28 2020-10-01 The Regents Of The University Of California Methods and systems for sequencing cell-free microbiome dna
US20220162592A1 (en) * 2019-04-08 2022-05-26 Zymo Research Corporation Duplex-specific nuclease depletion for purification of nucleic acid samples
BR112021006173A2 (pt) * 2019-04-29 2021-11-16 Illumina Inc Métodos para a identificação e análise de micro-organismos viáveis e/ou proliferativos e para determinação da eficácia de um agente antimicrobiano na modulação do crescimento e da proliferação de micro-organismos em uma amostra
US11396679B2 (en) 2019-05-31 2022-07-26 Universal Diagnostics, S.L. Detection of colorectal cancer
EP4041888A4 (en) 2019-10-11 2023-11-01 Guardant Health, Inc. USE OF CELLULAR BACTERIAL NUCLEIC ACIDS FOR CANCER DETECTION
US11898199B2 (en) 2019-11-11 2024-02-13 Universal Diagnostics, S.A. Detection of colorectal cancer and/or advanced adenomas
EP4010489A1 (en) * 2019-12-04 2022-06-15 Illumina, Inc. Preparation of dna sequencing libraries for detection of dna pathogens in plasma
EP4077659A1 (en) * 2019-12-16 2022-10-26 QIAGEN GmbH Enrichment method
CN111254190B (zh) * 2020-01-20 2021-02-23 中国医学科学院病原生物学研究所 一种血浆病毒组学的纳米孔三代测序检测方法
US20230132281A1 (en) * 2020-02-14 2023-04-27 Rhode Island Hospital Rna sequencing to diagnose sepsis
CN111239392B (zh) * 2020-02-26 2023-04-21 浙江诺迦生物科技有限公司 一种新型冠状病毒肺炎(covid-19)血清学诊断试剂盒
JP7522431B2 (ja) * 2020-04-01 2024-07-25 国立大学法人北海道大学 動物試料からのウイルス検出方法
EP4286538A3 (en) * 2020-04-06 2024-03-20 Octant, Inc. Pathogen diagnostic test
US12467096B2 (en) 2020-05-15 2025-11-11 Universal Diagnostics, S.A. Methods and systems for identifying methylation biomarkers
EP4150121A1 (en) * 2020-06-30 2023-03-22 Universal Diagnostics, S.A. Systems and methods for detection of multiple cancer types
CN117897504A (zh) * 2021-02-12 2024-04-16 凯锐思公司 患有莱姆病的对象中的微生物无细胞核酸的宏基因组下一代测序
US20220315986A1 (en) * 2021-04-01 2022-10-06 Diversity Arrays Technology Pty Limited Processes for enriching desirable elements and uses therefor
CN113201599B (zh) * 2021-06-03 2023-03-31 北京大学人民医院 一种基于PCR和nanopore测序检测脑脊液感染哪些病原体的方法
CN113684249B (zh) * 2021-08-25 2024-10-22 广州达安基因股份有限公司 一种rna和dna二代测序文库的构建方法及二代测序盒
CN115786547A (zh) * 2022-09-29 2023-03-14 武汉明德生物科技股份有限公司 用于病原微生物检测的引物组、试剂盒及其应用方法
CN115786548A (zh) * 2022-09-29 2023-03-14 武汉明德生物科技股份有限公司 用于病原微生物耐药基因检测的引物组、试剂盒及方法
US12606872B2 (en) 2022-11-16 2026-04-21 Universal Diagnostics, S.A. Methods for stratification and early detection of advanced adenoma and/or colorectal cancer using DNA methylation markers
AU2024246833A1 (en) 2023-03-31 2025-09-25 Toray Industries, Inc. Pharmaceutical composition for treating and/or preventing cancer
WO2024220566A1 (en) * 2023-04-18 2024-10-24 Karius, Inc. Sample processing to enrich for select species of nucleic acids
WO2025137492A1 (en) * 2023-12-22 2025-06-26 Siemens Healthcare Diagnostics Inc. Method for enriching pathogen dna and implementation thereof
CN118165982A (zh) * 2024-04-17 2024-06-11 上海麦克林生化科技股份有限公司 Rna快速提取试剂盒及其应用

Family Cites Families (78)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9220773D0 (en) 1992-10-02 1992-11-18 Mini Agriculture & Fisheries Detection of antigens and nucleic acids
US6759217B2 (en) 1996-03-26 2004-07-06 Oncomedx, Inc. Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer
USRE39920E1 (en) 1997-05-30 2007-11-13 Xenomics, Inc. Methods for detection of nucleic acid sequences in urine
US6753137B2 (en) 2001-01-31 2004-06-22 The Chinese University Of Hong Kong Circulating epstein-barr virus DNA in the serum of patients with gastric carcinoma
US20050202414A1 (en) 2001-11-15 2005-09-15 The Regents Of The University Of California Apparatus and methods for detecting a microbe in a sample
US7727720B2 (en) * 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
US20040209299A1 (en) 2003-03-07 2004-10-21 Rubicon Genomics, Inc. In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA
US20060073506A1 (en) 2004-09-17 2006-04-06 Affymetrix, Inc. Methods for identifying biological samples
ITRM20050068A1 (it) * 2005-02-17 2006-08-18 Istituto Naz Per Le Malattie I Metodo per la rivelazione di acidi nucleici di agenti patogeni batterici o di parassiti nelle urine.
ITRM20050067A1 (it) 2005-02-17 2006-08-18 Istituto Naz Per Le Malattie I Metodo per la rivelazione di acidi nucleici virali o di origine virale nelle urine.
EP2351857B1 (en) 2005-02-17 2014-10-29 TrovaGene, Inc. Compositions and methods for detecting pathogen specific nucleic acids in urine
WO2006089203A2 (en) 2005-02-17 2006-08-24 Istituto Nazionale Per Le Malattie Infettive Irccs Lazzaro Spallanzani Compositions and methods for detecting viral specific nucleic acids in urine
US7914982B2 (en) 2005-02-17 2011-03-29 Trovagene, Inc. Methods for detecting pathogen specific nucleic acids in urine
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US8532930B2 (en) 2005-11-26 2013-09-10 Natera, Inc. Method for determining the number of copies of a chromosome in the genome of a target individual using genetic data from genetically related individuals
EP3617321B1 (en) * 2006-05-31 2024-10-23 Sequenom, Inc. Kit for the extraction and amplification of nucleic acid from a sample
HUE019019T5 (en) 2007-08-22 2017-04-28 Trovagene Inc Methods for Using miRNA for In vivo Cell Death Detection
WO2009032781A2 (en) * 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for universal size-specific polymerase chain reaction
DE102007041864B4 (de) * 2007-09-04 2012-05-03 Sirs-Lab Gmbh Verfahren zum Nachweis von Bakterien und Pilzen
EP2201143B2 (en) 2007-09-21 2016-08-24 Katholieke Universiteit Leuven Tools and methods for genetic tests using next generation sequencing
AU2009215193A1 (en) 2008-02-12 2009-08-20 Pacific Biosciences Of California, Inc. Compositions and methods for use in analytical reactions
EP2853601B1 (en) * 2008-07-18 2016-09-21 TrovaGene, Inc. Methods for PCR-based detection of "ultra short" nucleic acid sequences
WO2011015944A2 (en) 2009-08-06 2011-02-10 Cedars-Sinai Medical Center Use of free dna as an early predictor of severity in acute pancreatitis
EP3783110B1 (en) * 2009-11-05 2022-11-23 The Chinese University Of Hong Kong Fetal genomic analysis from a maternal biological sample
JP2013509883A (ja) 2009-11-06 2013-03-21 ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ 臓器移植患者における移植片拒絶反応の非侵襲的診断方法
WO2011100617A2 (en) 2010-02-12 2011-08-18 Life Technologies Corporation Nucleic acid, biomolecule and polymer identifier codes
WO2011143659A2 (en) * 2010-05-14 2011-11-17 Fluidigm Corporation Nucleic acid isolation methods
US20120077185A1 (en) 2010-08-06 2012-03-29 Tandem Diagnostics, Inc. Detection of genetic abnormalities and infectious disease
EP2426217A1 (en) 2010-09-03 2012-03-07 Centre National de la Recherche Scientifique (CNRS) Analytical methods for cell free nucleic acids and applications
US20120100549A1 (en) * 2010-10-01 2012-04-26 Ibis Biosciences, Inc. Targeted genome amplification methods
WO2012088348A2 (en) 2010-12-23 2012-06-28 Sequenom, Inc. Fetal genetic variation detection
EP2694680A4 (en) 2011-04-01 2014-09-10 Occam Biolabs Inc METHOD AND KITS FOR DETECTION OF CELL-FREE NURVIVAL-SPECIFIC NUCLEIC ACIDS
WO2012159023A2 (en) 2011-05-19 2012-11-22 Virginia Commonwealth University Gut microflora as biomarkers for the prognosis of cirrhosis and brain dysfunction
EP2718862B1 (en) 2011-06-06 2018-10-31 Koninklijke Philips N.V. Method for assembly of nucleic acid sequence data
US20130024127A1 (en) 2011-07-19 2013-01-24 John Stuelpnagel Determination of source contributions using binomial probability calculations
GB201115095D0 (en) 2011-09-01 2011-10-19 Singapore Volition Pte Ltd Method for detecting nucleosomes containing nucleotides
CA3069089A1 (en) 2011-09-22 2013-03-28 Lineage Biosciences, Inc. Compositions and methods for analyzing heterogeneous samples
WO2013052907A2 (en) 2011-10-06 2013-04-11 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CA2861856C (en) 2012-01-20 2020-06-02 Sequenom, Inc. Diagnostic processes that factor experimental conditions
HK1211624A1 (en) 2012-01-27 2016-05-27 The Board Of Trustees Of The University Of The Leland Stanford Junior University Methods for profiling and quantitating cell-free rna
EP2820129A1 (en) * 2012-03-02 2015-01-07 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP3305918B1 (en) * 2012-03-05 2020-06-03 President and Fellows of Harvard College Methods for epigenetic sequencing
US9892230B2 (en) 2012-03-08 2018-02-13 The Chinese University Of Hong Kong Size-based analysis of fetal or tumor DNA fraction in plasma
CA2866324C (en) 2012-03-13 2019-01-15 The Chinese University Of Hong Kong Methods for analyzing massively parallel sequencing data for noninvasive prenatal diagnosis
EP3741871A3 (en) 2012-04-19 2021-02-17 The Medical College of Wisconsin, Inc. Highly sensitive surveillance using detection of cell free dna
EP2653562A1 (en) 2012-04-20 2013-10-23 Institut Pasteur Anellovirus genome quantification as a biomarker of immune suppression
EP2847353B1 (en) 2012-05-10 2022-01-19 The General Hospital Corporation Methods for determining a nucleotide sequence
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
JP2015522260A (ja) 2012-06-15 2015-08-06 ハリー スティリ, 疾患または状態を検出する方法
WO2014019275A1 (en) 2012-07-31 2014-02-06 Bgi Shenzhen Co., Limited Noninvasive detection of fetal health status
WO2014029792A1 (en) 2012-08-21 2014-02-27 Qiagen Gmbh Virus particle stabilisation and method for isolating viral nucleic acids
US9316802B2 (en) 2012-08-24 2016-04-19 Commscope Technologies Llc Optical fiber cable having reinforcing layer of tape heat-bonded to jacket
CN104781421B (zh) 2012-09-04 2020-06-05 夸登特健康公司 检测稀有突变和拷贝数变异的系统和方法
US20140066317A1 (en) 2012-09-04 2014-03-06 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
EP2728014B1 (en) 2012-10-31 2015-10-07 Genesupport SA Non-invasive method for detecting a fetal chromosomal aneuploidy
GB201302900D0 (en) 2013-02-19 2013-04-03 Genome Res Ltd Nucleic acid marker molecules for identifying and detecting cross contaminationof nucleic acid samples
US20140242582A1 (en) 2013-02-28 2014-08-28 Ariosa Diagnostics, Inc. Detection of genetic abnormalities using ligation-based detection and digital pcr
CN114574581A (zh) 2013-03-15 2022-06-03 夸登特健康公司 检测稀有突变和拷贝数变异的系统和方法
WO2014151117A1 (en) 2013-03-15 2014-09-25 The Board Of Trustees Of The Leland Stanford Junior University Identification and use of circulating nucleic acid tumor markers
WO2014145078A1 (en) 2013-03-15 2014-09-18 Verinata Health, Inc. Generating cell-free dna libraries directly from blood
WO2014179805A1 (en) 2013-05-03 2014-11-06 Webroot Inc. Method and apparatus for providing forensic visibility into systems and networks
US9499870B2 (en) * 2013-09-27 2016-11-22 Natera, Inc. Cell free DNA diagnostic testing standards
US20150119260A1 (en) * 2013-10-18 2015-04-30 National Taiwan University Circulating cancer biomarker and its use
AU2014346562B2 (en) 2013-11-07 2018-11-29 The Board Of Trustees Of The Leland Stanford Junior University Cell-free nucleic acids for the analysis of the human microbiome and components thereof
WO2015085105A1 (en) * 2013-12-04 2015-06-11 University Of Alaska Fairbanks Methods and compositions for enriching non-host sequences in host samples
JP6873921B2 (ja) 2015-05-18 2021-05-19 カリウス・インコーポレイテッド 核酸の集団を濃縮するための組成物および方法
EP3978627A1 (en) 2016-03-25 2022-04-06 Karius, Inc. Methods using synthetic nucleic acid spike-ins
BR112019002679A2 (pt) 2016-08-08 2019-05-14 Karius, Inc. redução de sinal de ácidos nucleicos contaminantes
WO2018045359A1 (en) 2016-09-02 2018-03-08 Karius, Inc. Detection and treatment of infection during pregnancy
WO2018191563A1 (en) 2017-04-12 2018-10-18 Karius, Inc. Sample preparation methods, systems and compositions
CA3082601A1 (en) 2018-03-16 2019-09-19 Karius, Inc. Sample series to differentiate target nucleic acids from contaminant nucleic acids
EP4700161A3 (en) 2018-11-21 2026-03-11 Karius, Inc. Direct-to-library methods, systems, and compositions
CN121575489A (zh) 2018-11-21 2026-02-27 卡里乌斯公司 感染性疾病的检测和预测
US20220195496A1 (en) 2020-12-17 2022-06-23 Karius, Inc. Sequencing microbial cell-free dna from asymptomatic individuals
WO2022140302A1 (en) 2020-12-21 2022-06-30 Karius, Inc. Sequencing microbial cell-free nucleic acids to detect inflammation, secondary infection, and disease severity
WO2022150725A1 (en) 2021-01-11 2022-07-14 Karius, Inc. Rapid, non-invasive detection and serial monitoring of infections in subjects using microbial cell-free dna sequencing
CN117897504A (zh) 2021-02-12 2024-04-16 凯锐思公司 患有莱姆病的对象中的微生物无细胞核酸的宏基因组下一代测序

Also Published As

Publication number Publication date
JP2021112200A (ja) 2021-08-05
EP4450636A3 (en) 2025-01-01
IL255647A (en) 2018-01-31
CN107922971A (zh) 2018-04-17
JP7769480B2 (ja) 2025-11-13
AU2016264102A1 (en) 2017-12-21
EP4450636A2 (en) 2024-10-23
CA2986036C (en) 2022-07-26
BR112017024747A2 (pt) 2018-11-13
HK1251946A1 (en) 2019-05-03
JP6873921B2 (ja) 2021-05-19
EP3298169B1 (en) 2024-10-02
US20260002197A1 (en) 2026-01-01
JP2023110928A (ja) 2023-08-09
JP2018520652A (ja) 2018-08-02
EP3298169A1 (en) 2018-03-28
US12291739B2 (en) 2025-05-06
US11111520B2 (en) 2021-09-07
US20220154246A1 (en) 2022-05-19
ZA201708264B (en) 2022-03-30
EP3298169A4 (en) 2018-10-24
CA2986036A1 (en) 2016-11-24
US20170016048A1 (en) 2017-01-19
WO2016187234A1 (en) 2016-11-24

Similar Documents

Publication Publication Date Title
ZA201708264B (en) Compositions and methods for enriching populations of nucleic acids
IL287586A (en) Methods and preparations for enriching breeding products
IL300438B1 (en) Compositions and methods for inhibiting lipoprotein A gene expression
EP3183367A4 (en) Compositions and methods for enrichment of nucleic acids
PT3325669T (pt) Composições e métodos de análise de rna
ZA201707981B (en) Compositions of obeticholic acid and methods of use
PT3882357T (pt) Métodos e composições para análise de ácidos nucleicos
IL249615A0 (en) Stereochemically enriched preparations for the administration of nucleic acids
IL254518A0 (en) Preparations and methods for suppressing the expression of the factor xii gene
ZA201507006B (en) Compositions and methods of nucleic acid-targeting nucleic acids
ZA201803723B (en) Compositions and methods for efficient targeting of transgenes
SG11201708741YA (en) Compositions and methods for biological production of methionine
PL3349743T3 (pl) Sposoby i kompozycje do hamowania oddziaływania dcn1-ubc12
IL255998A (en) Preparations and methods for suppressing the expression of the hif2alpha strain
IL260122A (en) Methods and compositions for assembly of nucleic acids
ZA201800175B (en) Methods and compositions for selective regulation of protein expression
SG11201803284YA (en) Novel method of use and compositions
IL240734A0 (en) Methods and compositions for preparing nucleic acids
GB201803240D0 (en) Methods and compositions for enriching nucleic acids
GB201509023D0 (en) Compositions and methods of production thereof
GB201408203D0 (en) Methods and compositions for identifying nucleic acids
GB201408204D0 (en) Methods and compositions for identifying nucleic acids
GB201408205D0 (en) Methods and compositions for identifying nucleic acids
GB201408207D0 (en) Methods and compositions for identifying nucleic acids
GB201511863D0 (en) Pharmaceutical compositions comprising hexaminolevulnic acid and methods of using the same