IE62803B1 - An isoelectric focusing process and a means for carrying out said process - Google Patents

An isoelectric focusing process and a means for carrying out said process

Info

Publication number
IE62803B1
IE62803B1 IE106788A IE106788A IE62803B1 IE 62803 B1 IE62803 B1 IE 62803B1 IE 106788 A IE106788 A IE 106788A IE 106788 A IE106788 A IE 106788A IE 62803 B1 IE62803 B1 IE 62803B1
Authority
IE
Ireland
Prior art keywords
isoelectric
amphoteric
gradients
flow
membranes
Prior art date
Application number
IE106788A
Other languages
English (en)
Other versions
IE881067L (en
Inventor
Daniel Michel Faupel
Pier Giorgio Righetti
Original Assignee
Ciba Geigy Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB878708746A external-priority patent/GB8708746D0/en
Application filed by Ciba Geigy Ag filed Critical Ciba Geigy Ag
Publication of IE881067L publication Critical patent/IE881067L/xx
Publication of IE62803B1 publication Critical patent/IE62803B1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
IE106788A 1987-04-11 1988-04-08 An isoelectric focusing process and a means for carrying out said process IE62803B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB878708746A GB8708746D0 (en) 1987-04-11 1987-04-11 Electrophoretic process
GB878728289A GB8728289D0 (en) 1987-04-11 1987-12-03 Isoelectric focusing process & means for carrying out said process

Publications (2)

Publication Number Publication Date
IE881067L IE881067L (en) 1988-10-11
IE62803B1 true IE62803B1 (en) 1995-03-08

Family

ID=26292133

Family Applications (1)

Application Number Title Priority Date Filing Date
IE106788A IE62803B1 (en) 1987-04-11 1988-04-08 An isoelectric focusing process and a means for carrying out said process

Country Status (12)

Country Link
US (2) US4971670A (c_deeref_Disk_and_Scratch_Disk_Pools_and_Their_Defaults.html)
EP (1) EP0287513B1 (c_deeref_Disk_and_Scratch_Disk_Pools_and_Their_Defaults.html)
JP (1) JPH0781987B2 (c_deeref_Disk_and_Scratch_Disk_Pools_and_Their_Defaults.html)
CA (1) CA1335805C (c_deeref_Disk_and_Scratch_Disk_Pools_and_Their_Defaults.html)
DE (1) DE3876273T2 (c_deeref_Disk_and_Scratch_Disk_Pools_and_Their_Defaults.html)
DK (1) DK174962B1 (c_deeref_Disk_and_Scratch_Disk_Pools_and_Their_Defaults.html)
ES (1) ES2037273T3 (c_deeref_Disk_and_Scratch_Disk_Pools_and_Their_Defaults.html)
FI (1) FI97893C (c_deeref_Disk_and_Scratch_Disk_Pools_and_Their_Defaults.html)
GR (1) GR3007124T3 (c_deeref_Disk_and_Scratch_Disk_Pools_and_Their_Defaults.html)
HK (1) HK192495A (c_deeref_Disk_and_Scratch_Disk_Pools_and_Their_Defaults.html)
IE (1) IE62803B1 (c_deeref_Disk_and_Scratch_Disk_Pools_and_Their_Defaults.html)
PT (1) PT87210B (c_deeref_Disk_and_Scratch_Disk_Pools_and_Their_Defaults.html)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5173160A (en) * 1991-02-27 1992-12-22 Board Of Regents, The University Of Texas System Analysis of carrier ampholytes using immobilized ph gradients
US5323846A (en) * 1992-01-14 1994-06-28 Fotodyne Incorporated Fluid circulator and temperature regulator
GB2267502B (en) * 1992-05-28 1997-01-22 Aligena Ag Polymeric reaction products for use in immobilized buffered gels and membranes
US6375899B1 (en) 1993-11-01 2002-04-23 Nanogen, Inc. Electrophoretic buss for transport of charged materials in a multi-chamber system
US6319472B1 (en) 1993-11-01 2001-11-20 Nanogen, Inc. System including functionally separated regions in electrophoretic system
US6129828A (en) * 1996-09-06 2000-10-10 Nanogen, Inc. Apparatus and methods for active biological sample preparation
US20040077074A1 (en) * 1993-11-01 2004-04-22 Nanogen, Inc. Multi-chambered analysis device
US6331274B1 (en) 1993-11-01 2001-12-18 Nanogen, Inc. Advanced active circuits and devices for molecular biological analysis and diagnostics
US6225059B1 (en) 1993-11-01 2001-05-01 Nanogen, Inc. Advanced active electronic devices including collection electrodes for molecular biological analysis and diagnostics
US5437774A (en) * 1993-12-30 1995-08-01 Zymogenetics, Inc. High molecular weight electrodialysis
US5480526A (en) * 1994-06-07 1996-01-02 Bio-Rad Laboratories, Inc. Methods for the desalting of biological samples: a simple approach to eliminate disturbances in isoelectric focusing caused by the presence of salts
US6071394A (en) 1996-09-06 2000-06-06 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US7857957B2 (en) * 1994-07-07 2010-12-28 Gamida For Life B.V. Integrated portable biological detection system
US6403367B1 (en) * 1994-07-07 2002-06-11 Nanogen, Inc. Integrated portable biological detection system
CN1051245C (zh) * 1995-01-20 2000-04-12 清华大学 制备型等电点电泳分离方法及设备
IT1272932B (it) * 1995-01-24 1997-07-01 Pier Giorgio Righetti Reattore ad enzima immobilizzato
US5540826A (en) * 1995-03-15 1996-07-30 Protein Technologies, Inc. Multi-channel separation device
US7824532B2 (en) 1995-04-26 2010-11-02 Life Technologies Corporation Apparatus and method for electrophoresis
EP0836619B1 (en) * 1995-07-05 2004-08-25 AVENTIS BULK S.p.A. Purification of dalbaheptide antibiotics by isoelectric focusing
RU2140073C1 (ru) * 1998-01-30 1999-10-20 Стоянов Александр Владимирович Способ формирования сред с заданным профилем ph
DE19831210A1 (de) * 1998-07-03 2000-01-05 Wita Gmbh Wittmann Inst Of Tec Verfahren und Vorrichtung zur zwei-dimensionalen Trennung von Biomolekülen
AUPP521298A0 (en) * 1998-08-12 1998-09-03 Life Therapeutics Limited Purification of fibrinogen
SE9803224D0 (sv) 1998-09-23 1998-09-23 Amersham Pharm Biotech Ab Method for separation of macromolecules
US6267579B1 (en) * 1998-12-23 2001-07-31 Clinical Laboratory Development Group, Inc. Apparatus for making a gradient gel
AUPP790698A0 (en) 1998-12-23 1999-01-28 Life Therapeutics Limited Separation of microorganisms
US20050224355A1 (en) * 1999-12-23 2005-10-13 Brendon Conlan Removal of biological contaminants
AUPP790898A0 (en) 1998-12-23 1999-01-28 Life Therapeutics Limited Renal dialysis
AUPP971399A0 (en) 1999-04-12 1999-05-06 Life Therapeutics Limited Separation of plasma components
US6284115B1 (en) 1999-09-21 2001-09-04 Agilent Technologies, Inc. In-line flow through micro-dialysis apparatus and method for high performance liquid phase separations
US6758953B2 (en) 1999-10-28 2004-07-06 Nathan A. Thomas Multistage electrophoresis apparatus and method of use for the separation and purification of cells, particles and solutes
JP2003514829A (ja) * 1999-11-15 2003-04-22 プロテオム システムズ リミテッド 多室電気泳動
AU1599501A (en) * 1999-11-16 2001-05-30 James T. Champagne Solution based two-dimensional separation and detection of amphoteric substances
US7077942B1 (en) * 1999-12-23 2006-07-18 Gradipore Limited Removal of biological contaminants
US20080096284A1 (en) * 2000-02-08 2008-04-24 Regents Of The University Of Michigan Protein separation and analysis
AU3812000A (en) * 2000-03-15 2001-09-24 Proteosys Ag Micropreparative isoelectric focussing
US6638408B1 (en) * 2000-04-03 2003-10-28 The Wistar Institute Method and device for separation of charged molecules by solution isoelectric focusing
AUPQ691400A0 (en) * 2000-04-14 2000-05-11 Life Therapeutics Limited Separation of micromolecules
ATE449200T1 (de) 2000-04-18 2009-12-15 Gradipore Ltd Trennung und behandlung von proben durch elektrophorese
AUPQ697300A0 (en) 2000-04-18 2000-05-11 Life Therapeutics Limited Separation apparatus
GB0010957D0 (en) * 2000-05-05 2000-06-28 Novartis Ag Compound & method
US6537434B1 (en) 2000-07-21 2003-03-25 Large Scale Proteomics Corporation First dimension electrophoresis separation method and apparatus
US6562213B1 (en) * 2000-08-30 2003-05-13 Ethrog Biotechnology Ltd. Electrophoresis apparatus for simultaneous loading of multiple samples
US6923896B2 (en) * 2000-09-22 2005-08-02 The Texas A&M University System Electrophoresis apparatus and method
AUPR051500A0 (en) 2000-09-29 2000-10-26 Proteome Systems Ltd Electrophoresis system
US20030019753A1 (en) * 2000-10-05 2003-01-30 David Ogle Multi-port separation apparatus and method
AUPR222300A0 (en) * 2000-12-21 2001-01-25 Life Therapeutics Limited Electrophoresis device and method
WO2002071024A2 (en) * 2001-03-08 2002-09-12 Ethrog Biotechnology Ltd. Apparatus and method for electrophoresis
AU2002322513B2 (en) * 2001-07-16 2008-02-28 Protein, Forest, Inc. Arrays of buffers for analysing biomolecules by their isoelectric point
GB0121189D0 (en) * 2001-08-31 2001-10-24 Diagnoswiss Sa Apparatus and method for separating an analyte
US6887362B2 (en) * 2002-02-06 2005-05-03 Nanogen, Inc. Dielectrophoretic separation and immunoassay methods on active electronic matrix devices
HUE027134T2 (en) * 2002-09-11 2016-10-28 Chugai Pharmaceutical Co Ltd Method for protein purification
WO2004048398A1 (ja) * 2002-11-28 2004-06-10 Arkray Inc. 核酸の濃縮精製方法および装置
US7622028B2 (en) * 2003-05-09 2009-11-24 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
WO2004104548A2 (en) * 2003-05-09 2004-12-02 Invitrogen Corporation Solution phase electrophoresis device, components, and methods
JP2007504215A (ja) * 2003-09-03 2007-03-01 バイオフォームズ 生体分子を急速に結晶化するための方法及び装置
US20050103629A1 (en) * 2003-10-07 2005-05-19 Tom Diller Isoelectric focusing gels and methods of use thereof
EP1696026A4 (en) * 2003-11-10 2007-05-09 Arkray Inc PROCESS FOR CONCENTRATING AND PURIFYING NUCLEIC ACID AND APPARATUS THEREOF
US20050197496A1 (en) * 2004-03-04 2005-09-08 Gtc Biotherapeutics, Inc. Methods of protein fractionation using high performance tangential flow filtration
US7833625B2 (en) * 2004-03-17 2010-11-16 Bio-Rad Laboratories, Inc. Materials, methods and systems for separating and identifying proteins from mixtures
US7815783B2 (en) * 2004-03-17 2010-10-19 Bio-Rad Laboratories, Inc. Multi-compartment filter and method of filtering using same
US20050249667A1 (en) * 2004-03-24 2005-11-10 Tuszynski Jack A Process for treating a biological organism
US7407816B2 (en) * 2004-05-07 2008-08-05 Gentius, Inc Isoelectric particles and uses thereof
DE212005000044U1 (de) * 2004-08-25 2007-04-05 Agilent Technologies Inc., Santa Clara Elektrophoretische Separation in einem bewegten Fluid
US20060130159A1 (en) * 2004-12-09 2006-06-15 Nick Masiello Method of purifying recombinant MSP 1-42 derived from Plasmodium falciparum
JP5123847B2 (ja) 2005-05-25 2013-01-23 バイオ−ラド ラボラトリーズ インコーポレイテッド 流体工学装置
CA2616976C (en) * 2005-07-28 2012-08-28 Bio-Rad Laboratories, Inc. Separation of proteins based on isoelectric point using solid-phase buffers
US7531632B2 (en) * 2006-02-16 2009-05-12 Gtc Biotherapeutics, Inc. Clarification of transgenic milk using depth filtration
US7964075B2 (en) 2006-04-27 2011-06-21 Ge Healthcare Bio-Sciences Ab Electrodic bridge
US20080220442A1 (en) * 2006-12-06 2008-09-11 Proteinics Difference detection methods using isoelectric focusing chips
US8366899B2 (en) * 2007-06-22 2013-02-05 Massachusetts Institute Of Technology Isoelectric focusing systems and methods
CN101896250A (zh) * 2007-10-09 2010-11-24 达尔豪西大学 提纯分子的装置
US8282803B2 (en) 2009-03-25 2012-10-09 Bio-Rad Laboratories, Inc. Isoelectric focusing tray and electrode assembly for alternate gel strip orientations
CN104023551A (zh) 2011-11-04 2014-09-03 生物辐射实验室股份有限公司 细胞组分的同时纯化
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
CN104024396A (zh) * 2011-11-04 2014-09-03 生物辐射实验室股份有限公司 采用pH的电子控制的亲和方法及组合物
EP2788746B1 (en) 2011-11-22 2018-10-24 Stephen G. Haralampu Stopped-flow, micro-fluidic device and method for the charge-based separation of complex analyte mixtures
US9658195B2 (en) 2012-02-15 2017-05-23 Bio-Rad Laboratories, Inc. Electronic control of pH and ionic strength
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
US9671368B2 (en) * 2013-05-10 2017-06-06 The Regents Of The University Of California Two-dimensional microfluidic devices and methods of using the same
CN105358228A (zh) 2013-07-05 2016-02-24 法国血液分割暨生化制品实验室 亲和层析基质
MX368107B (es) 2013-07-12 2019-09-18 Genentech Inc Explicacion de la optimizacion de entrada de la cromatografia de intercambio ionico.

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3539493A (en) * 1967-08-31 1970-11-10 Canal Ind Corp Apparatus for preparative electrophoresis on gel support media
US3844925A (en) * 1973-07-02 1974-10-29 Center For Blood Res Molecular fractionation
SE415731B (sv) * 1977-04-26 1980-10-27 Pharmacia Fine Chemicals Ab Vattenloslig amfolyt for separationsendamal samt sett att framstella densamma
DE2861803D1 (en) * 1977-06-15 1982-07-01 Nat Res Dev Electrophoresis membranes, their use in a separation method and separation apparatus
EP0103965A2 (en) * 1982-08-20 1984-03-28 Imperial Chemical Industries Plc Electrofocusing apparatus
US5114555A (en) * 1988-01-05 1992-05-19 Monsanto Company Continuous isoelectric separation

Also Published As

Publication number Publication date
US4971670A (en) 1990-11-20
DE3876273T2 (de) 1993-05-27
FI97893C (fi) 1997-03-10
PT87210B (pt) 1993-11-30
US5082548A (en) 1992-01-21
DE3876273D1 (de) 1993-01-14
DK191388D0 (da) 1988-04-08
JPS63263457A (ja) 1988-10-31
IE881067L (en) 1988-10-11
FI881652A0 (fi) 1988-04-08
CA1335805C (en) 1995-06-06
EP0287513A2 (en) 1988-10-19
HK192495A (en) 1995-12-29
PT87210A (pt) 1989-05-12
DK191388A (da) 1988-10-12
GR3007124T3 (c_deeref_Disk_and_Scratch_Disk_Pools_and_Their_Defaults.html) 1993-07-30
ES2037273T3 (es) 1993-06-16
FI881652A7 (fi) 1988-10-12
JPH0781987B2 (ja) 1995-09-06
FI97893B (fi) 1996-11-29
EP0287513B1 (en) 1992-12-02
EP0287513A3 (en) 1990-10-10
DK174962B1 (da) 2004-03-29

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