HUP9904579A2 - Eljárás nukleinsavak átvitelére alkalmas kompozíciók előállítására - Google Patents

Eljárás nukleinsavak átvitelére alkalmas kompozíciók előállítására

Info

Publication number
HUP9904579A2
HUP9904579A2 HU9904579A HUP9904579A HUP9904579A2 HU P9904579 A2 HUP9904579 A2 HU P9904579A2 HU 9904579 A HU9904579 A HU 9904579A HU P9904579 A HUP9904579 A HU P9904579A HU P9904579 A2 HUP9904579 A2 HU P9904579A2
Authority
HU
Hungary
Prior art keywords
nucleic acids
cationic lipid
subject
transfer
preparing compositions
Prior art date
Application number
HU9904579A
Other languages
English (en)
Inventor
Tsiala Boukhnikachvili
Joël Vacus
Original Assignee
Rhone-Poulenc Rorer S.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rhone-Poulenc Rorer S.A. filed Critical Rhone-Poulenc Rorer S.A.
Publication of HUP9904579A2 publication Critical patent/HUP9904579A2/hu
Publication of HUP9904579A3 publication Critical patent/HUP9904579A3/hu

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6905—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a colloid or an emulsion
    • A61K47/6907—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit the form being a colloid or an emulsion the form being a microemulsion, nanoemulsion or micelle
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers
    • A61K9/1272—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers with substantial amounts of non-phosphatidyl, i.e. non-acylglycerophosphate, surfactants as bilayer-forming substances, e.g. cationic lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle

Abstract

A találmány tárgya eljárás nűkleinsavak átvitelére szőlgáló kőmpőzícióelőállítására, amely magában főglalja egy nűkleinsav érintkeztetésétegy katiőnős lipiddel. Az eljárást az jellemzi, hőgy az érintkeztetéselőtt a katiőnős lipidet egy melegítési lépésnek vetik alá. Atalálmány tárgyát képezi egy eljárás nűkleinsavak sejtekbe való invitrő vagy ex vivő átvitelére is, amely magában főglalja az említettnűkleinsav érintkeztetését egy előzetesen melegített katiőnőslipidszűszpenzióval, és a sejtek inkűbálását a keletkezettnűkleőlipidkőmplexszel. A találmány tárgya még egy kőmpőzíciónűkleinsavak átvitelére, amelyre az jellemző, hőgy alapvetőenérzéketlen a szérűm gátló hatására és hőgy az előzőleg említetteljárás szerint kapták. ŕ
HU9904579A 1996-10-08 1997-10-03 Method for preparing compositions for transferring nucleic acids HUP9904579A3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9612259A FR2754272B1 (fr) 1996-10-08 1996-10-08 Procede de preparation de compositions pour le transfert d'acides nucleiques

Publications (2)

Publication Number Publication Date
HUP9904579A2 true HUP9904579A2 (hu) 2000-05-28
HUP9904579A3 HUP9904579A3 (en) 2001-06-28

Family

ID=9496468

Family Applications (1)

Application Number Title Priority Date Filing Date
HU9904579A HUP9904579A3 (en) 1996-10-08 1997-10-03 Method for preparing compositions for transferring nucleic acids

Country Status (15)

Country Link
US (1) US6156338A (hu)
EP (1) EP0948638A1 (hu)
JP (1) JP2001501641A (hu)
KR (1) KR20000048958A (hu)
AU (1) AU723371B2 (hu)
BR (1) BR9712210A (hu)
CA (1) CA2267187A1 (hu)
CZ (1) CZ119299A3 (hu)
FR (1) FR2754272B1 (hu)
HU (1) HUP9904579A3 (hu)
IL (1) IL129336A0 (hu)
NO (1) NO991383D0 (hu)
SK (1) SK43199A3 (hu)
WO (1) WO1998015639A1 (hu)
ZA (1) ZA979031B (hu)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2750704B1 (fr) 1996-07-04 1998-09-25 Rhone Poulenc Rorer Sa Procede de production d'adn therapeutique
DE69924975T2 (de) * 1998-02-17 2005-10-06 University College Cardiff Consultants Ltd., Cardiff Verfahren und kit, um biologische substanzen in plasmamembran und/oder zytosol einzuführen
CN1981873A (zh) * 2000-06-09 2007-06-20 莱古伦公司 制备微胶粒的方法和所述微胶粒的用途
US20030235916A1 (en) * 2002-06-14 2003-12-25 Monahan Sean D. Novel methods for the delivery of polynucleotides to cells

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4873089A (en) * 1985-07-12 1989-10-10 Cornell Research Foundation Inc., Cornell University Proteoliposomes as drug carriers
JPS63277618A (ja) * 1987-03-31 1988-11-15 Noebia:Kk リポソ−ムの製造方法
WO1989005636A1 (en) * 1987-12-22 1989-06-29 The Liposome Company, Inc. Spontaneous vesiculation of multilamellar liposomes
FR2714830B1 (fr) * 1994-01-10 1996-03-22 Rhone Poulenc Rorer Sa Composition contenant des acides nucléiques, préparation et utilisations.
FR2727679B1 (fr) * 1994-12-05 1997-01-03 Rhone Poulenc Rorer Sa Nouveaux agents de transfection et leurs applications pharmaceutiques

Also Published As

Publication number Publication date
CA2267187A1 (fr) 1998-04-16
AU723371B2 (en) 2000-08-24
NO991383L (no) 1999-03-22
IL129336A0 (en) 2000-02-17
SK43199A3 (en) 2000-04-10
NO991383D0 (no) 1999-03-22
KR20000048958A (ko) 2000-07-25
CZ119299A3 (cs) 1999-07-14
ZA979031B (en) 1998-04-23
JP2001501641A (ja) 2001-02-06
US6156338A (en) 2000-12-05
EP0948638A1 (fr) 1999-10-13
HUP9904579A3 (en) 2001-06-28
WO1998015639A1 (fr) 1998-04-16
FR2754272A1 (fr) 1998-04-10
FR2754272B1 (fr) 1998-11-13
AU4559197A (en) 1998-05-05
BR9712210A (pt) 1999-08-31

Similar Documents

Publication Publication Date Title
Cox et al. recA protein of Escherichia coli promotes branch migration, a kinetically distinct phase of DNA strand exchange.
Richet et al. Purification and characterization of a DNA-dependent ATPase from Escherichia coli.
DE68925002D1 (de) Verfahren zur Herstellung von optisch-aktiven alpha-substituierten organischen Säuren und Mikroorganismen und Enzyme, die dafür verwendet werden.
Niyogi et al. A novel oligoribonuclease of Escherichia coli. I. Isolation and properties.
KR920701478A (ko) 핵산서열의 증폭방법
HUP9901198A2 (hu) Eljárás L-aminosavak előállítására
DE3177286T2 (de) Genetisch modifizierte mikroorganismen fuer die massenherstellung amylolytischer enzyme und verfahren zu deren herstellung.
NO996541L (no) Forbedret metode for overfoering av nukleinsyre til celler i multicellulaere, eukaryotiske organismersamt kombinasjon for dette
ES2104611T3 (es) Procedimiento de amplificacion mejorado de acidos nucleicos.
FR2637612B1 (fr) Sequences de nucleotides codant pour une proteine a activite ureasique
IT1075203B (it) Processo per preparare proteine immobilizzate in particolare per legare enzimi ad un supporto insolubile e relativo prodotto
FI970115A (fi) Nukeliinihappoja sisältävä valmiste, sen valmistus ja käytöt
HUP9900808A2 (hu) Eljárás nukleozid-5'-foszfát-észterek előállítására
BR9705813B1 (pt) processo para produzir éster nucleosìdeo-5'-fosfato, fosfatase ácida mutante, gene isolado codificando fosfatase ácida mutante.
IT8547624A0 (it) Ceppi batterici particolarmente utili per la produzione di vaccini per il trattamento di malattie intestinali e simili e metodo di ingegneria genetica per la produzione dei detti vaccini
HUP9904579A2 (hu) Eljárás nukleinsavak átvitelére alkalmas kompozíciók előállítására
BRPI0006915B8 (pt) processo para preparação de l-aminoácidos
DE69632303D1 (de) Poly(organo)phosphazene in synthetischen transfektions-systemen
FR2563533B1 (fr) Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues
HUP9902320A2 (hu) Enzimkombinációk osztódásban levő sejtek elpusztítására
WO2001040519A8 (en) Methods for identifying modulators for balance related disorders
DE60043090D1 (de) Verfahren zur Herstellung von grossen Schmiedestücken
AU7210196A (en) In situ hybridization to detect specific nucleic acid sequences in eucaryotic samples
DE68910087D1 (de) Verfahren zur Herstellung von harten, ein Enzym enthaltenden Kandis.
HUP0202524A2 (hu) Enzimmel kezelt fermentált termék és előállítási eljárása