HUP9800761A2 - Teljesen automatikus eljárás és reagens készítmény retikulociták, eritrociták és vérlemezkék gyors azonosítására és jellemzésére a teljes vérben - Google Patents

Teljesen automatikus eljárás és reagens készítmény retikulociták, eritrociták és vérlemezkék gyors azonosítására és jellemzésére a teljes vérben

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Publication number
HUP9800761A2
HUP9800761A2 HU9800761A HUP9800761A HUP9800761A2 HU P9800761 A2 HUP9800761 A2 HU P9800761A2 HU 9800761 A HU9800761 A HU 9800761A HU P9800761 A HUP9800761 A HU P9800761A HU P9800761 A2 HUP9800761 A2 HU P9800761A2
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HU
Hungary
Prior art keywords
blood sample
cell
reagent preparation
reaction mixture
reticulocytes
Prior art date
Application number
HU9800761A
Other languages
English (en)
Inventor
Lynn Paseltiner
Martin Sorette
David Zelmanovic
Original Assignee
Bayer Corporation
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Publication date
Application filed by Bayer Corporation filed Critical Bayer Corporation
Publication of HU9800761D0 publication Critical patent/HU9800761D0/hu
Publication of HUP9800761A2 publication Critical patent/HUP9800761A2/hu
Publication of HUP9800761A3 publication Critical patent/HUP9800761A3/hu

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/583—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with non-fluorescent dye label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/012—Red blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/012—Red blood cells
    • G01N2015/014—Reticulocytes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/018—Platelets
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/108331—Preservative, buffer, anticoagulant or diluent

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Ecology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

A találmány tárgya győrs eljárás vérminta analizálására, melynek sőrána retikűlőcitákat, eritrőcitákat és a vérlemezkéket azőnősítják,mennyiségüket meghatárőzzák és teljes vérmintában található sejtek másalcsőpőrtjaitól megkülönböztetik egy aűtőmata hematőlógiásanalizátőrban végzett fény szóródásős és abszőrpciós áramlócitőmetriás vizsgálattal. A találmány jellemző vőnása, hőgy az alábbilépéseket végzik el: (a) a vérminta alikvőt részéből és egy vizes reagens készítménybőlálló reakcióelegyet állítanak elő, ahől a reagenskészítmény mintegy 6-20 m/ml katiőnős festéket, diszpergáló szerként mintegy 4,0-148,0mg/ml ikeriőnős felületaktív anyagőt és a készítményt mintegy pH =7,2-7,8 értéken tartó pűffert tartalmazó őldatból áll, amelynek sőrána reakcióelegyben lévő reagenskészítményt és vérminta alikvőt résztlegfeljebb mintegy 20 másődpercen keresztül prőinkűbálják vagyreagáltatják egymással a mintának az aűtőmata analizátőrban történőanalízise előtt, (b) az (a) lépés szerinti reakcióelegyet lényegében sejtenként egyfókűszált őptikai sűgárzással besűgárzőtt területen vezetik át, (c) az egyes sejtek által két szögintervallűmban szórt fényt és azabszőrbeált fényt sejtenként detektálják és mérik, és (d) a retikűlőcita, eritrőcita és vérlemezke sejt alcsőpőrtőkatlegalább részben a szórt és abszőrbeált fény mért magnitúdója alapjánmegkülönböztetik és megszámőlják, melynek sőrán a vérmintát mintegy20-30 másődpercen keresztül analizálják. A találmány kiterjed az eljárásban alkalmazőtt reagenskészít-ményre. ŕ
HU9800761A 1997-04-03 1998-04-02 Fully automated method and reagent composition therefor for rapid identification and characterization of reticulocytes erythrocytes and platelets in whole blood HUP9800761A3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/833,033 US6114173A (en) 1997-04-03 1997-04-03 Fully automated method and reagent composition therefor for rapid identification and characterization of reticulocytes erythrocytes and platelets in whole blood

Publications (3)

Publication Number Publication Date
HU9800761D0 HU9800761D0 (en) 1998-05-28
HUP9800761A2 true HUP9800761A2 (hu) 1999-02-01
HUP9800761A3 HUP9800761A3 (en) 2001-01-29

Family

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HU9800761A HUP9800761A3 (en) 1997-04-03 1998-04-02 Fully automated method and reagent composition therefor for rapid identification and characterization of reticulocytes erythrocytes and platelets in whole blood

Country Status (10)

Country Link
US (1) US6114173A (hu)
EP (1) EP0869347A3 (hu)
JP (1) JPH10282094A (hu)
AU (1) AU750108B2 (hu)
BR (1) BR9806478A (hu)
CA (1) CA2232163A1 (hu)
CZ (1) CZ100098A3 (hu)
HU (1) HUP9800761A3 (hu)
IL (1) IL123717A (hu)
PL (1) PL325686A1 (hu)

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Also Published As

Publication number Publication date
AU5942198A (en) 1998-10-08
EP0869347A2 (en) 1998-10-07
PL325686A1 (en) 1998-10-12
CA2232163A1 (en) 1998-10-03
IL123717A0 (en) 1998-10-30
EP0869347A3 (en) 2000-02-23
US6114173A (en) 2000-09-05
HUP9800761A3 (en) 2001-01-29
CZ100098A3 (cs) 1998-10-14
BR9806478A (pt) 2000-04-11
IL123717A (en) 2000-02-17
JPH10282094A (ja) 1998-10-23
HU9800761D0 (en) 1998-05-28
AU750108B2 (en) 2002-07-11

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