HUP0101959A2 - Ligandumok szűrése és tervezése röntgenkrisztallográfia segítségével - Google Patents

Ligandumok szűrése és tervezése röntgenkrisztallográfia segítségével

Info

Publication number
HUP0101959A2
HUP0101959A2 HU0101959A HUP0101959A HUP0101959A2 HU P0101959 A2 HUP0101959 A2 HU P0101959A2 HU 0101959 A HU0101959 A HU 0101959A HU P0101959 A HUP0101959 A HU P0101959A HU P0101959 A2 HUP0101959 A2 HU P0101959A2
Authority
HU
Hungary
Prior art keywords
ligands
biomolecule
ligand
target molecule
bind
Prior art date
Application number
HU0101959A
Other languages
English (en)
Inventor
Celerino Abad-Zapatero
Jonathan Greer
Vicki L. Nienaber
Daniel W. Norbeck
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Publication of HUP0101959A2 publication Critical patent/HUP0101959A2/hu
Publication of HUP0101959A3 publication Critical patent/HUP0101959A3/hu

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • C—CHEMISTRY; METALLURGY
    • C30—CRYSTAL GROWTH
    • C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
    • C30B7/00—Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
    • G01N23/20—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by using diffraction of the radiation by the materials, e.g. for investigating crystal structure; by using scattering of the radiation by the materials, e.g. for investigating non-crystalline materials; by using reflection of the radiation by the materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/972—Plasminogen activators
    • G01N2333/9723—Urokinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Materials Engineering (AREA)
  • Metallurgy (AREA)
  • Organic Chemistry (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A röntgenkrisztallográfia a célmolekulához való kötődési képességükkövetkeztében olyan vegyületek szkrínelésére alkalmazható, amelyek egycél-biomolekulának nem ismert ligandumai. Az eljárás (továbbiakban<CrystaLEADTM<) abból áll, hogy a cél-biomolekulából egy kristálytnyernek; a célmolekulát egy vagy több tesztmintával érintkeztetik,amelyek a célmolekula potenciális ligandumai; és meghatározzák, hogykialakult-e egy ligandum/biomolekula komplex. A célmolekulát apotenciális ligandumokkal különféle módszerekkel érintkeztethetik, ígypéldául a kristályt átitatják egy vagy több potenciális ligandumoldatával, vagy a biomolekulát egy vagy több potenciális ligandumjelenlétében együttkristályosítják. Egy másik kiviteli mód szerint aligandum/receptor komplexekről kapott szerkezeti információkatalkalmazzák olyan új ligandumok tervezésére, amelyek erősebbenkötődnek, specifikusabban kötődnek, jobb biológiai aktivitássalrendelkeznek vagy nagyobb biztonsági profillal rendelkeznek, mint azismert ligandumok. A találmány alkalmas továbbá biológiailag aktívmaradékok felismerésére és tervezésére. Egy további kiviteli módszerint a könnyen hozzáférhető aktív hellyel rendelkező biomolekulakristályokat úgy állítják elő, hogy a biomolekulát lebonthatóligandummal együtt kristályosítják, és a ligandumot lebontják. Ó
HU0101959A 1998-03-06 1999-03-05 Ligand screening and design by x-ray crystallography HUP0101959A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3618498A 1998-03-06 1998-03-06
PCT/US1999/004967 WO1999045379A2 (en) 1998-03-06 1999-03-05 Ligand screening and design by x-ray crystallography

Publications (2)

Publication Number Publication Date
HUP0101959A2 true HUP0101959A2 (hu) 2001-09-28
HUP0101959A3 HUP0101959A3 (en) 2003-10-28

Family

ID=21887121

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0101959A HUP0101959A3 (en) 1998-03-06 1999-03-05 Ligand screening and design by x-ray crystallography

Country Status (18)

Country Link
US (1) US6297021B1 (hu)
EP (1) EP1068531B1 (hu)
JP (1) JP2002506206A (hu)
KR (1) KR20010041653A (hu)
CN (2) CN101221138A (hu)
AT (1) ATE375510T1 (hu)
AU (3) AU2887099A (hu)
BG (1) BG104802A (hu)
BR (1) BR9908563A (hu)
CA (1) CA2321968A1 (hu)
DE (1) DE69937292T2 (hu)
HU (1) HUP0101959A3 (hu)
IL (1) IL137758A0 (hu)
NO (1) NO20004433L (hu)
PL (1) PL343264A1 (hu)
SK (1) SK13242000A3 (hu)
TR (4) TR200101127T2 (hu)
WO (2) WO1999045389A2 (hu)

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US7250305B2 (en) 2001-07-30 2007-07-31 Uab Research Foundation Use of dye to distinguish salt and protein crystals under microcrystallization conditions
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US7806980B2 (en) 2000-12-23 2010-10-05 Elan Pharmaceuticals, Inc. Method for crystallizing human beta secretase in complex with an inhibitor
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US7384773B1 (en) * 2001-05-10 2008-06-10 Pfizer Inc Crystal of HIV protease-cleaved human beta secretase and method for crystallization thereof
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US20030211538A1 (en) * 2001-12-03 2003-11-13 Ming Luo CAP-Gly domain structure and uses thereof
US20040171062A1 (en) * 2002-02-28 2004-09-02 Plexxikon, Inc. Methods for the design of molecular scaffolds and ligands
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Also Published As

Publication number Publication date
BR9908563A (pt) 2000-11-21
US6297021B1 (en) 2001-10-02
PL343264A1 (en) 2001-07-30
CN101221138A (zh) 2008-07-16
DE69937292T2 (de) 2008-07-03
WO1999045389A3 (en) 2000-02-10
TR200101200T2 (tr) 2002-02-21
EP1068531A2 (en) 2001-01-17
AU2003255167A1 (en) 2003-11-13
CA2321968A1 (en) 1999-09-10
TR200002572T2 (tr) 2000-11-21
WO1999045389A2 (en) 1999-09-10
CN100354627C (zh) 2007-12-12
AU767991B2 (en) 2003-11-27
NO20004433L (no) 2000-11-06
AU2987899A (en) 1999-09-20
SK13242000A3 (sk) 2001-03-12
DE69937292D1 (de) 2007-11-22
BG104802A (bg) 2001-04-30
ATE375510T1 (de) 2007-10-15
IL137758A0 (en) 2001-10-31
TR200101129T2 (tr) 2002-06-21
KR20010041653A (ko) 2001-05-25
EP1068531B1 (en) 2007-10-10
AU2887099A (en) 1999-09-20
WO1999045379A2 (en) 1999-09-10
TR200101127T2 (tr) 2002-06-21
CN1299467A (zh) 2001-06-13
HUP0101959A3 (en) 2003-10-28
JP2002506206A (ja) 2002-02-26
WO1999045379A3 (en) 1999-11-25
NO20004433D0 (no) 2000-09-05

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