HUP0101523A2 - Eljárás Caenorhabditis elegans ballisztikus transzformálására - Google Patents
Eljárás Caenorhabditis elegans ballisztikus transzformálásáraInfo
- Publication number
- HUP0101523A2 HUP0101523A2 HUP0101523A HUP0101523A2 HU P0101523 A2 HUP0101523 A2 HU P0101523A2 HU P0101523 A HUP0101523 A HU P0101523A HU P0101523 A2 HUP0101523 A2 HU P0101523A2
- Authority
- HU
- Hungary
- Prior art keywords
- caenorhabditis elegans
- microprojectiles
- nucleic acid
- nematodes
- ballistic transformation
- Prior art date
Links
- 241000244203 Caenorhabditis elegans Species 0.000 title abstract 2
- 230000009466 transformation Effects 0.000 title 1
- 241000244206 Nematoda Species 0.000 abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- 239000011859 microparticle Substances 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
- A01K67/0335—Genetically modified worms
- A01K67/0336—Genetically modified Nematodes, e.g. Caenorhabditis elegans
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
- C12N15/895—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection using biolistic methods
Abstract
A találmány tárgya eljárás nukleinsav bevitelére fonálférgekbe,különösen Caenorhabditis elegansba, mely során az említett férgetmikrolövedékek sokaságával bombázzák. A nukleinsavat amikrolövedékekre viszik fel, vagy a bombázás előtt fonálférgeketvonják be vele, és csupasz mikrolövedékekkel bombáznak. A találmánykiterjed a fenti módon előállított, nukleinsavat és mikrorészecskéksokaságát tartalmazó fonálférgekre is. Ó
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB9806211.0A GB9806211D0 (en) | 1998-03-23 | 1998-03-23 | Ballistic transformation of C elegans |
GBGB9819868.2A GB9819868D0 (en) | 1998-09-11 | 1998-09-11 | Ballistic transformation of C.elegans |
PCT/EP1999/001903 WO1999049066A1 (en) | 1998-03-23 | 1999-03-22 | Method for ballistic transformation of caenorhabditis elegans |
Publications (1)
Publication Number | Publication Date |
---|---|
HUP0101523A2 true HUP0101523A2 (hu) | 2001-09-28 |
Family
ID=26313337
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HUP0101523 HUP0101523A2 (hu) | 1998-03-23 | 1999-03-22 | Eljárás Caenorhabditis elegans ballisztikus transzformálására |
Country Status (10)
Country | Link |
---|---|
US (2) | US6433247B1 (hu) |
EP (1) | EP1064394B8 (hu) |
JP (1) | JP2002507428A (hu) |
CN (1) | CN1302335A (hu) |
AU (1) | AU757182B2 (hu) |
CA (1) | CA2325579A1 (hu) |
DE (1) | DE69923546T2 (hu) |
HU (1) | HUP0101523A2 (hu) |
PL (1) | PL343071A1 (hu) |
WO (1) | WO1999049066A1 (hu) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7612194B2 (en) * | 2001-07-24 | 2009-11-03 | Monsanto Technology Llc | Nucleic acid sequences from Diabrotica virgifera virgifera LeConte and uses thereof |
EP1732379B1 (en) | 2004-04-09 | 2013-11-06 | Monsanto Technology LLC | Compositions and methods for control of insect infestations in plants |
ES2614943T3 (es) | 2005-09-16 | 2017-06-02 | Monsanto Technology Llc | Procedimientos para el control genético de infestaciones de insectos en plantas y composiciones de los mismos |
JP2010075057A (ja) | 2008-09-24 | 2010-04-08 | Sumitomo Chemical Co Ltd | 昆虫由来の電位依存性カリウムチャネル活性に関わる有害生物の生理状態に変化を与える薬剤 |
KR101334403B1 (ko) * | 2011-05-25 | 2013-11-29 | 포항공과대학교 산학협력단 | 형질전환 예쁜꼬마선충 및 이를 이용하여 당 대사 조절물질을 검색하는 방법 |
HUP1100657A2 (en) * | 2011-11-29 | 2013-06-28 | Eotvos Lorand Tudomanyegyetem | Transgenic caenorhabditis elegans |
CN104472438B (zh) * | 2014-11-21 | 2017-06-16 | 苏州大学 | 一种线虫物理损伤的方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5120657A (en) | 1986-12-05 | 1992-06-09 | Agracetus, Inc. | Apparatus for genetic transformation |
WO1993024626A1 (en) * | 1992-05-28 | 1993-12-09 | Scientific Dimensions Usa, Inc. | Transgenic animal production with biolistically transformed spermatozoa |
-
1999
- 1999-03-22 EP EP99917833A patent/EP1064394B8/en not_active Expired - Lifetime
- 1999-03-22 CN CN99806532A patent/CN1302335A/zh active Pending
- 1999-03-22 CA CA002325579A patent/CA2325579A1/en not_active Abandoned
- 1999-03-22 JP JP2000538026A patent/JP2002507428A/ja active Pending
- 1999-03-22 DE DE69923546T patent/DE69923546T2/de not_active Expired - Fee Related
- 1999-03-22 PL PL99343071A patent/PL343071A1/xx unknown
- 1999-03-22 HU HUP0101523 patent/HUP0101523A2/hu unknown
- 1999-03-22 US US09/273,965 patent/US6433247B1/en not_active Expired - Fee Related
- 1999-03-22 AU AU35976/99A patent/AU757182B2/en not_active Ceased
- 1999-03-22 WO PCT/EP1999/001903 patent/WO1999049066A1/en active IP Right Grant
-
2002
- 2002-07-02 US US10/188,329 patent/US20030145340A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP1064394A1 (en) | 2001-01-03 |
US20030145340A1 (en) | 2003-07-31 |
AU757182B2 (en) | 2003-02-06 |
EP1064394B8 (en) | 2005-03-30 |
PL343071A1 (en) | 2001-07-30 |
AU3597699A (en) | 1999-10-18 |
DE69923546D1 (de) | 2005-03-10 |
CN1302335A (zh) | 2001-07-04 |
CA2325579A1 (en) | 1999-09-30 |
WO1999049066A1 (en) | 1999-09-30 |
JP2002507428A (ja) | 2002-03-12 |
US6433247B1 (en) | 2002-08-13 |
DE69923546T2 (de) | 2006-02-16 |
EP1064394B1 (en) | 2005-02-02 |
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