HUE033878T2 - Eljárás mikotoxinok enzimes lebontására szolgáló adalékanyag elõállítására és adalékanyag és annak alkalmazása - Google Patents

Description

ilJ&AsMIKOmXiNOK IMZIMES LEBONTÁSÁRA SZOLGÁIÉ ABALÉKANlMs előállítására éb adalékanyag és annak: alkalmazása

The present invontion ï9hmtQ-M:Mâtin for Ée. enäpMtlc ^ipítóílöa^fftöMJaMfes. in & vcptafolsraw material and mixtures coakumng vegetable t&vt materials, a» well as thf use of these.

Mycotoxiaa very frequently occur on agricultural vegetable product sod, depending onThe -type^faiyeo*ox-ins, India severe economic damage, in particular, m the foods produced: Earn agricultural products and even in animals and humans fed with such foods, said damage being sicdemejy stasifalddprm^erons motlKKis have aheady been developed, trying so detoxify or degrade, ortender harmless, suPh mytatoxins in order to inhibit agy dMUtge ceased by myeotoxins m the Skids of animal and human nutrition, animal breeding, food and teed pocessiihg and die like. iCnotvn myeotoxins comprise a jdarsMiy ipf steatmaily ihiaoMatsb: snysotoxins such as, for instance, in-morpsine, among which ftsrnotnsih Hl IsÄ«trooáitistusdöy oecuirmg toxin of the group. There are, however, nm mérous derivatives sad related, molecular sä to exhibit notions e Heels in humans and animals.

It feilne* that : hsmortMos impair Üt® »phingoüpid metabolism by interacting wuh the enxyme ceramide synthase, Sphingishpids oust only are eompunénts of cell Âëmbranes. but also play an important role as signal and; : messenger molecules In many eletneoisry cbliulhr processes like cell gfpwtb,: ceil migration and cell binding, hr inBamssatOfy iproeesses and intracellular mmspbtfpssedufex. One to this hsphumem of the sphingolipid metaho-· ifem, jltmonisists have been m&drr«s$onsÄ :¾ .íttortót «Hoëfs oh viiritms airtesd specks áhd stsn httmatss. i: could, thus,,demonstrated thatdbmorasins have: sauce.rupnk eHïsets in rodetlts, Md, based on epiddmiblögte tisda, they have been : associated1 with esophageal cancer and neural tub« delects m tenransv They have been held response hie for the typical toxicosis caused by pulmonary edemas, for festanos, m various animal species: such as, swine, Itt this context, femMisios constitute an almost ubiquitous contamination source on various cereal crops, in particular com as well as msta and vepiahies, attd itis skonglysreptive sfkot mlatlhg to the health Of hafuatçs and animat» is not to he neglected.

The microhm! degradation of fumonisins has already been described Its EP-A I MO OSA, according to which microorganisms are used to detoxify famonisins at^ luummsltt dertvmivesby Milng to feeds detoxify ing bacteria oryeasts selected: from preciselyMined: strains fete

Catabolic metalfeie path fWÚsMQtegmiál&^ikimf ftMÂM'Éi genes and engymes responsible them tor -have mm^hm described too, äs, EL 0 BSt m* #r $mmm* 4ta*r8*» feteisMdt oxtiying compositions and -tmtymm-mnä m above all preduced in tpmspnfc plants in which the Mmőámtkm of Istuonislns is epeted using an amine oxidase that inquires molecular oxygen for Its gnxymatie activity.

Moreover,; WO 2ÔP4/08S1M and saunomutasss as weil: as eotliposl- uons and Methods for the enzymatic detoxification to detoxify, in particular, uminatediAbfeiS, s,g,: íníuonísihs> In deaminase actsvity are used for detoxification,

Frosu WO 00/04158, me use of Punonisiti-degradittg amine Oxidases in ihe production id' foods or feeds and in the processing of vegetable rosy n-steals has become known.

Mitherso knosvst methods, however, have in common that, in order to detoxify mycotoxins, they »«quire sio-fealsr öxy#P» tbr the described catabolic metabolic paths, yes she amine oxidases, which are particularly required, cannot work undor oxygemmdepe&dent conditions. The use of such genes and enryroes for the detoxification of ibeds, fer iasiause hr fee bt|pfeve: fesets rfeè, is of É.e Äfefefe%: ft^pfesreé eovh .tournent in fee digestiv« IfUdscifand «mmes fens exhibiting; m> activity.

The invention ttow eien to provide: 8« additi ve fer the «axymatic degradation of fomonisfes, fey vfeieh It is feasible in a «sfe and reliable manner to ciegfede # detofely s«di mycotoxte

Vegetable raw materials in this eottfexf feelede eetefes or cereal products, gstsasss^ fetife or vegetables snälh» ^«mediate products >x;nmlrtmg these sufesiaoces for the production of foods and feeds: ssfeh as, fcfÄ«, silage, trait mash or the like,

Addfeves in few context fedifefc fesd afejfe vas, fenti additives as yfelltts additivaaihr ihe production of hip* ethanol:.

With sncfe a ptnesdiaxt It Is possible toMsfein fee sphingoUpid metabolite, wldfet Is Impaired By an intents-tioa of tumofesiss with: feofenxysne: eemmidsyhifesse,. and .at the same time to biologically degrade fee snmmbsins fete finally, technological npplfestk>ns fer defexifleatfett eatt he achieved, at tins procedure is sise appilcshfe in » broader technical scope,: so that with tits poenbnrc accord ing do ins invention it w possible to safely and veil ably produce tsycotofemfrsc ptxtnets;

Thotthcfeicíscid sequences used for degradation ofmycotoxias or the ensytnes expressed in prokaryotic and euktsryotic host eels by »fed nncleie sell sequstfeenwK! efeblyttfedly acfetg: äh 8«ibxygriwn^i#ÄtPt5t«OMig.ak are listed fee io v.··,

Nudele acids Sequences; SIS:: ID m 1 .-·y.:,yD 1 ( Fatn {üenaoni.ui.o cof.-alsoλχsu-} qq.’G. c.= ros:..ο?., :.;.·,. \·.·::·..· .όρ,

TcrrCDGCaATCRGs:aaA£:TTCTfe:C^rDDTCCTC:GysXXIX":AChKr:aTAC:AT(tfeIAGA<:RT(:(KIDA1N;TC(:AACTÖfesC CCfeCCCdtXISAÂCCChbkJGTCGAÂdC^oCffbGdTCÎGGTCtTGTCCiCTGhhhDOTTTTTGGCGTGCfetiGOArAAhCGACR rV'aAGTGDysvscmGGGAGGTTaTTGCGACGAAGGSíytGOCCXG'GGXGGGGTGooG:rOM'GíV.rrv,e..AGtx.Aaímo:n:;.i ·χ.: '.r... CGGACXGGGexG'GGGGaTTGGXGACAAXGöGGCKX'íAOOilT^ OGGOGTGOT£rXXGG\CCGGGACGAAíIAATATCGCATTGTG'.f:ívXG^GG£Gfe^vGAGth>woGoereo:cA^

MlGGGGAAGSGAÍACCGG&amp;GGGGX'f(tísASGGCOAShSRT:GGTGCCíS“':rÄA'1' ΊΆ'i..dfOa^îQ^Gs.^Aww'^.^o· iartw):i.v*\.

OAOTACG&amp;&amp;AGGTGnGTGCXXtAGGTTGGGGCACATTCGCGGIGGTTGGTXoATGGGàtTGAsX..o;-.:...:.,.,:\x\.\.\:."'.. · Gj·»., IwG hGT'I':y;feTGCíCCDCACC:TTtvICC?GX^f:«C^T^flX:tChtGdlTCGGAíGtCG(Gd:C<:<:GAt:ATTHhhhfAGC:GG('rx:tAS C<:v:fe’rCA:7d;:G0:ÂGAGC?aGTCGGGCTCGÂhAAACTGCTGGGGTCGTTGGïCGCTÂTTGGCGDA':CTCGGÎCGAACAAÂT í&amp;^$i»A£CőMtÍ&amp;sí3!Éi&amp;&amp;SdXt*ööT^^

GCCGGAGCGTGGTrOAAATTCGGArGCiACGCtCCXCCACAXTGTCGGCATTGAGCACCGArGCTGCGATClAXGOGGTGCTC CACGAGAAGCGCCCAA?CGTCXXlGfdlIXAÍAÍCGüGCCTGGAAG?TGGCCGXC:..A‘ o rc>d: t. t.· i".r; ooGo .. λ .:· λ ·..·· .·· :-·'·" ·''

A^GGATGGGCGCXX.PGCCGGTCGCiNfrGCQATAGGCAGAGGTATGGTGGGTTGCCAGCGCXCGAAGACTGAACGrGCCG (XXX:CGACC;GG(X:nTGGGrAAGAGßCC1'C0«ÄGTC«A1-yc;XX^CGCTTTCTGGÄC:AG(:CGGGTGGAGAGTGGGAi:(:(Xrr

GGVGGGGAAGGAGGAAATr:GaTCGCGCCCTÏ(:A(:CTT<:A(XtAGATGGCGA7X'(;ACXXîAAAA(:TGAhÂCCCC:GG(GWGAAG GGGGGGfXiGMiGACGGGGaCGGGG^^^^ CGnXTGCAGAGCCGGIGGGCXIGGAAAÍTGAATTGAGOGTCGCCTGACGGGCGGGG'í r.GAAGv. ··.. · ;.λ,Λο·/·>Α':··.:·'.· λ .; wso··:

CGXGGXTATCTCGCGAXCGGG?CGCGCGGAAGCÎÎXGG0C0XCGA1XXXX;TCATAT?CÎCXGCCCAGG AGAT

âCîvCâGGGGGCGGSGGGGÂGTCGGCGGGTCÎG'îâCGGGGCÇGGTîGM:GTGGGGGâAGGGGGGGGCGGGC“:SîGCG.'GÇGS AGTCSeKmGGGGGGGTGCGj&amp;öA&amp;ÄACCT;rGGG1:GSfGfGG1:ÄGGTTCeCSGGÄMÖGMGfG(3GGTGTÄGÄGÄTTGAMC GTCCAGGGCGGCCTGCGfGGGGCGGGGGAGG:CCCG:GTGATTCGCSfGGG:CGÁGGG(:G:TGGGCfGGGIs:rArCGCGfCíáGGT GCCTGCGGGGGCflGfrCGGfGCGÂTArTGGÂX^MGGiGfGSGGÎGGGGGMsÂÇGGGGCÇÇGGGGCGAGCXGG^ÂGGCGGGCGGG eïGGCXrcGGGGG&amp;ÂArCGTGGGTCGGGGGGCGXÂGG^eGXÀAGGGGfCACGGGMGCGÀGGCGGiïCÂôCCKÿGXmCGTÂGIXm TGGGCGXCOX/GCTAXOGGAG^TGTGCCGXAGAG/XnXXGCGGCACGeAATGTTGGTCXiAGGCOXXXAAGTGTAGAACGv CAGACGAÍAA1A;x<X:GCGGGAXCGOGGAGÜCXXGXGACOGOOvCGTAGAGAAXGCGGTTOATXGTGAGAXXGrTTTCAC\: ÄGGGTGGÄGGIGAGGA^GGCCÄGäSGÄGGCGGÄGCGGTCG&amp;GTGGGGGCsCCÄGTGCCÄÄGGGGGGCCGGGGSQCCGGCÄG CGCCGCAXXXxyvXXlXXGXAXAATTCTCGATTTCGXCCAAGGGAAAOXXGCXlXACXAGCGACCGXXXACGATGGXAGCX GAX:GCArrGGTG1XX:ATGAGCGTCGCGA(XGXÄGGCXXGGGXXGXXXlGTTATCAXXGXGG;XTGCGGAAATTXrTGGAGCC AXX.XAACATGCGA.XAÍXTACGAATGCACCCAsGGXXQGA'GA^GGGAAA'AAGCATaGGG'XAAxXGs'vG^^XA'XívAG'G'XGCAGA^GTGAí' CGGACAXXfvAXXAGGGGCGCXXXGGXXOXXXXXXXAGOGXAGCXGAGGGGGGGGGXAGGGGXXAXXCACGCGCGGTCCX TCGSXAGCGGGGÎGGÀÂCGA.GGAGCGCGGAÀAGf1Acx:QmGÎGisAASG1GXX:;XAGCGGXXGCfCCGGGAA&amp;AAÂGGCGAr GCCXrrCAGXGGGCXXGACGXGAQXXGXCCGÖCGXAGQXXCCGCGAGAXGTGCGXXXAXCGGGCXCGXCCGTGAGGÍXXG TCGTAGCGGCGAXAXACGGGAsTATTGVäCGwGCGGGGATGi'vGAAvA;,AGGXAAA¥ÄCCCG'TX'Gv'i''«'CA'ivXiAiG'GüGGA'X'ACG-w TGGTGGXAX^AXGXTATTGGAvAGGTGGüGAGÖXAGXXX^AGCGGTGGXCGGCÁAáCA^AGTGGCGAí^AJCGA'í'GGAAga^V'G' GrXGGACCGGXGGXCGCCGCGGGTTGAAAGAGCCGACTGGAGGXCGGCXX;TGArGCC:TXGGA:XXCYXXGTCGXGGACC;:A AGGGGAXGXGGCCCGAAACGGGXXCGGXSeGGXXGGCGGCCGAGGCGGCGCCCGXCXGGXCXXCGACGCGGTTGAGCAXC GÍXAGGGGGAGGAGGTXGAXáfGGÁGGí3AGGaXGXGáAAffGGX<ACGAGGGAGGÍXJffGGGGXX€A:GGAGGAÍA3GGGtT OGGGGXTGAvXCGAGGCCyGGGXXGTXáXGvXGG'.;' XGXxAvvAÄAGXXOCCOG^rGXXxATXXAGAAXXxXXyXXGGxX:· ACTfGAAGGXGTGGTTGAAGAGGGGGAGGXGGXXGAGG’GGGTGGGCAAGGTXGGTCGGAGAXGCXXrCCTTCAACTGCXCG CXGGXXAGGGGXGTAXGCGGGGXGGGGGAAXXGAAGGCGTTCXGQXGGGGGüIACCGGTGAGGAGGAÍXTGGCXCGTTCG CCGGTCCGTGXCGGGIXXXXXCCGGCXaAGGXSXGGAXGCGGGAXCGCTXlXXXGAGCAGTGGCXGXGXXXGGXAATGCXsA GGGXseCGAGCGCTACXrGÜGCCGXGCAXáCfXfiGGCCXXGÜCGÁXXiXTTTCGAXXGXGXXCIXCCGATAeAXCTGCAGT íXXXÍACCGAAXXGXXXGGGAGXGCGXIXmGTCCCCXTGTXTCTXGACGGCGCGGTCXGXCAGCAGGGXCCGGTGGGAGG C^'AA\ÂXSTCGGGGvXAÂGiXAGXAAAAVAXXTGAÂGXï,ï,v^ÂAÂÏGCÂAX>XAXGG'^AAvvÎ''Î,A^vrTGCiAXXxX'wG^vA"i'A^sAA-:G GGGGCGGTAGCXGAAXGTGCGAAXAXTCAíXX;XÍXXTGCXGAACAGr\X;CGGCArGACGXíXX:XCAXCGGCCACGCGGTX GGGGGCXTCGACGTGGGXXXGGCGTCOCCOGCGCXGXAOCACCXXXXAGTTGGXTXAXXAXOGAGXvAGGC'AGGAOAGAC Χ:ΆA^Xí^sAi V- ί· V- : AXYyAy ÁXyÁAGAG· AAAvYX AAAG > vG A: : y .y.y. v.-v:·AyyvA:·· 'A. A :> ‘ ..:: : : ·: : \. y:- .y\.-AA\.- À y y ·νί'>-y :v.y:y. ;:X:A':’XXG:AACXTATTG':':A::TAGGGATG:X:':X.ATAGA;.-. y:AXX.X.A.XAIGyGXÿ:yGXÿy.y\..y. GC A.X. CG:AGGGy:yXGG.A.yXAG\.\G.:yAA.

AAGCGGGAXCGAvGGCAAÁGAAGAXTXGGGGGSXC^TGCGCGGGGGGGGTGXvCXXCAACTGAAGAGCAAGGGGCXGAGAG

;X;XGCCTGGAXGGCTGGCXXXX:GGGCGGGGGCGCXGGCGAyCAGCGT::XX:GAGAXAAGXrGXtCXGTCGAATGATGGTGA.A CíXTGXfCGAGCXXÍAGCSGGGGGGAGA'rGGGAlAGGGAI'IXXX^AAACXXiXfGXGCGCAGCAACGGAGAXGGGGTTGGAXA: CCGAXXXGCQXCCAGCXGCAAXCGCGGGGGäGCGGGXXäGGXGCGGGCXGAXGGXCAXGGAGGAGAXGCGGACGGGXCXG GXOXÇXAÇAXTXXvGXGCGGTGCGCGCGAACXCSXGCXGGGCGAGGGOCXSGTCCXCXXAXXClXCGX(XtGGGXXAGGTS ÎGG<XGGXSACAGCCGC:ÂAXAXGGCX::AGGGTGGG?X:TT:GAAGGGGGCGGAXAAGGTGXGGGCXiGGGTXAX:AGG:i:ATAATAÏ GCGXÇXTGCACGCGATCGvXAvvGXGXXTvCGGGCAGXXXGGGXXGXGXXCCACGGGGXXGXGCGAXGAa'AGGCGOOTX X GGCGCAXAAXAxGGTXG'XGCGCGCGGXAXGGGCACGCxCGAGÁMXXGrCGGÜGGXAXGGXAGGAXCGCXGXGXTTXXXvyG ACmCAGGGCAvAAXCGGCATGACXîGAîGX\XGCXX:AGAAAGGGGTGGGCGAGGXXGAGCATC;ATCTGCGAAGGAC(;GGTi:

ATGGrGICGCOOAGAAGTaXrtGCGCAxCAGCCGíXiGCTTCGATYG'CGGAyTGOCGíXrAACCGAOCA&amp;ATTTGGOGCATA

CGCGCACAXXxAGxCGAGATAGGGCxGCTOGTGGGOGTCCCXAATxCGiSCAGvGAGCGCGCGCCtGAXGAACTGGGGGA

ArTt;íGG(:G(;í:A(G:AACCGTCT:A;A(rr(;(}TGGCr:GTACAicccGcccGGAATí;A(:(x:G's:i'CGGí:G(:GTTCTC:fGAG/G'G'T

TTCTGCG:TGGGGGGGTTCGCCM'ÄA"^GCÄCCTvGGGvGG.TÄ&amp;ÄTASG:GGGTiGGG\&amp;GCGÄGGÄ#G^TCÄnGGÄTTCGTGA

xCxGXAAQAGXxXTAXCrTGGXAxAXACyGXAÍG\ATTAGAexGAXyGCGGAAÍCGGXCGACXX;GATXGGGGGGCAGGGAG

GACGGGGAAXTCTATGOGGAACGTCAGCGACAAGGCGCGGCXXXGíCGAGACCXrCAGCGTAGÍCGTCXX'GGCAATGAxCG

IXGGCACGGCXACCTxGATGGTGCyTGíGxATACAGCCCATCCrrCTGGGCGCCXJTTíyrAGAGGAGGGGeGTATTCCCGCO GAGGGGTxGGGATCGGCGGGAAGGGTGGAXÄTACTGGGGÄxGGevXsCGGGAXGArGGATCGGACGCGTTCTxATGÄÄGAi::

GGGATATCXGGGGGGGAiG\GGCGGGGCACfGXGGTXAATGGXCGCCGGAA’rGiyi:CTfCGGATTGACGTTGCGG:GGXTTGG AXXfGGCCATvGXGGCXXGGGGÄGCGGGÄGGGGQiGSCGCTGGAÄGGTCTTtCGGXGAGGÖGGSCGATCGTGÄTQÄXGAG^r 0/νΓΑχ;'ΓχΧ.χ.χ.. GGCCGxAx CGGCTG^X:XX:XGGXXAXxG\yΓGGGCAGΛXXxX’-xXoAxΛCCGCx:XAΓXAx A.CTxAXX Axxxxx·

GCCGACGGAGATTAxXCCGGQCTGGGGSAGCGCAGGCGGCTTCACGAyCCxGGCXIATTCTCGGGGOGXXCGGCGGGArOG

CGXCxOTGT A~;XC xGGTGGAG^GGGGiXΛGA:GXGXAxGGGGAGGAA A AXGAAGGAGGGaG aG.GGGaTG:G'G 'GA.G.. CGCGGCAG:G*G

GTGATTTCGAxGGCGGCAGTGGTxCAâTÎCTCGGGG01X<:XaAÎXXG:ATGGAGGÎATGGCG:;AGCGAGTGGCGGCGCAGC.A

CGGAXíXXGGGGAGAAAvGA£GGGT.AÍGGGCAXT1'CGGGG&amp;GTÍTGGXTTGCGAGGTAGGGGGGGCTTGGCTGGCCGCTX GGATCGGxGGGCGGGfeGGATATCGCTXGGCGXxAAxCGCTGGGAGCCTGCTxCX®GCGGGGAACGTGAXCÖCAXXGCXXÍ

GTGGCGGATGAGGCAAeCTGGTTTAXTGACGGXTCX:TGTGCTTTCGGCCTGTfCTGGTTGGCGAXGCAGOGCÍTCGAXAT GGGXX .1 XvG A:XXA;:oXxGAGXGAXaX. A:XXwOGxA:-.Á.X í. ÍAv.· X .:. XV .. AGA XXA\XA?X-XA:AGG,.G'GGG;ΧΧΛ;<TOG'GGíXXvA>G;:vAv GSCCCTTGTTGCXCTGTCGCT:rTGCCGGGGCGAGGGlG:TTGvGCTGGÄTGTTGGTGGGGAGT'rCGACvrrTGTTGCTGGCG AGCGC::x::TTCTGTATCXTTGCCCr:x;rrrGITTCAAOCGCGCGGAAAGGTGATCGCyGAAAOGG?GGACGTATGAAAAAG AGÔGAGOGGGGXXGGCGAXGACAXCGiXGsGTGAAGGTTCGTÂGCGCGGÇAAAGCGGCGGCGGAG'rCCTÂAÂAGCGÂGGGA GGTGT'rGC'TGGTyAGGAGTCGÏXGCTTGÂÏOCGÂCCGÂGÂGGGTGïTGGTCGAïCTAGACGGCGATCAGGGGGGGAiX'TA TCXG5ATTGCAGAGGAAGCGGGTGGCTCACGGTCGTCCGTCtA?{mTTTCTTxCCGAC{:AAGGAAGTGGCTGATCXX:GCXC AGGÁXGAAAA-XxG 'AXV.Í CAAXGXGGGGGGGGGGGAAXXGGvAGGGxGXAGTGGAjX'XXXXAA.AG Gs:iGí A'..XVvA/.vG^xX^Xj:V':í\.'rG>óCG\oxA>N-·

CTGAyCGGG3TXGGATCXG3ÄTCAGGGCGCGAGAGXAXTÄtMGAÖCCACCCGCCGGiXAXiX:AÄGCTXCTGXTGGöCGc;AXA X GGvA:X:G:®:'AGgGx\AAXAaGxGxXAXXX XA'AxAxAAGGAÍ Αχ Λ X'.-'.:XGG ΧΑχν· Χ.ν\Χ ΧΧΧΧ. XΧΆ :·. \.:· G·. Λ ΧΧΑ r::-ΑΧ G\-AG·.·. - Χ A - X TTTTCCATATGCCGCXAATGGAGAATÍGGXXACnxAxGXfCACGATCTGCrfCGCAATTCÍCGACGCGGTArGGGCCGTC TCCxTTCG€GGGfGCGGxGAAXxxAOGXGGGXXTTTC?xCGGGAGGGGGAXGCGGCyXGCAGTGCCTAXTGCCGACX€x;A TGTGvGCGAGGGAACGGGAGGAGGGfGAATCvGTxCAÄCGÄTATGCAGGAAXGXCCGTxGCGXTGAGTTCGGXXvXGAGT TGGGTGGGTXAGGAGGCCCCGCGAxAAAeGAAGGCTGTTCTGTCGAAGGGAXGTCGGCXGGXXCGACGAGGCCCxGGGM G'rGAGCCGCAAXCAACGAGGGAGAXGfvAACGTGGCCAGGAAGTTCAACTCGrC^GTTAClX'GATe’rGGGAfCAyxTGXtG GGGyGTATGAAACGGGAAŰxxTTAGCCAí:GCCGCGCGGaTxCTGAAxCAAXCGCAGCGCGGGCTCAGOCGGAG.AAXCCA:G GGÇCTÇGAGÀGTCTCG:!:GGGCGGÏCGGTÎG?XCGAGGGGAGCAGTCsX5XGGCTtGGGGAAAGSGOGGTÇGGGAAAGAGxT GGGGCGGGXGGCCGAGGGAGCGTTGGAAGXTGTGGAXAvGXCGGTGTXTGGGTCGGCCAAxGTGCGGGAGTTGGGGTGGA CAGACATCGG'GAXTGCC:GGTGXAGAGAGvGCCGCGxTGGArGTTGTC>::GGCGAGCxGGGGGCXTGXACAr®GA"'CAAXG'í'

CGGAGGGTGGGAGXGGGGATGCfTGACGxűGGGGCGGTGGixGGCTGGGGACGxGGTxGCGAGGGGCÍGiGGvGGAGTXCGG eAyGAGGAxTGAGAGCC"GXXÖCCAxCAÍXX:CTGGGGTTCGATGCGCfCCAGGACXíACCCGTXCGGCGXGGCAXGC€.íG;C:

ímSGCATGGGCxGGC«TCGCTCGiAGGrGCTTG.?GiTGGAGGG^X:GAAAGGTGAAAGCGTGATCGCCX;xTTCACCGXGGG

AGCCGGAXCCGCACGTTGCTCGArGGCGMCTCGCGCQCAACXATXXCGCGCTGGÄATGGCGGTMAXööxCGCGCmxCT GAeGACGGCG€XGGGAyTGAyCGAGGGGCAAGÏGGGTQTCGCTGTTAïGCCGGGGAXGGÏxAtGCvGCG(™CGGGTCGGx

CGGXGGyCGTCfQSGGCGCCGTCGGCGCGCCGSTCGxCGXACGCACGAGCGGGAxCGTxCAGCGCCGGiCCGGCxGCXViG /G/..//GA/GGGG'.///.X///'.//A///:C/XXAXG.XGGGi/XG,/.G.://.:/:.ÇCTGGA//://./../::CC//A//.././^ -'. G -.'CGC//'Λ

AGATGGnGCATCGÎGÂCACGCGTTCTAGGCGCC.TGCAGCATCGATGCACACGXTC.ATîCGAÎÎvGCTGAGAOAOOAACGC

GAAGXGACCGCTGGGtCAGAGOATAGCAGTCCA/XAAGGCGGGAGAGAAXÍGTGTGAAAGAGCA/X/XATOCC/G'GGCGCG

GGGGGGTCCX:cGGeTG(XX'GCGGCÁCGGGGCo?TGCCG'GGS1X:ASí:GTTTCCCÖGGGGGCGTCCGCCATGGCG:;'GGAGCÍT

GXOGGTTGQGGCXAOTGCCXXXGCCGOGGCXGCGCXXXCXGAeGATCCGAAGCTOGTGCGTCATACCGAGTeGGGCGüOG

GCGAGGGGGTCGAÎX3GÇGXCG00GAGAGTTTOTTGGGXâTXCCCÎXGGGGGCT0GGCGGGX€GGC0AGCTGÇSAXGGOGG CGGOCOGCTCCGCCGXGGGOeOGGGeGGGCACCAGGGAOGGGGGCCGCTTTGCGCCGGATTGGXTCGGGAXGGAGCGGC'r //A/:AGAGGGGAGGCGGGOXGCCGGGACGAGCGáAGAC XGCGΓνΧΧΑΓΟΤΟΧΑ/'Α/GTGG'./G/^'\. vCíy\AG^'.:s.>^Ai^u\rAAGGí

GGGCXA:TCGGCGTCATGAÍATGGGTTTAGGGGGGTGGG;rTTA(XGGGGGGTTCTGGGGCGGTGGGAXAT:rATGAX:GGGTCT

GCGCTCGCGCAGAAGGaCGGGGGGCGrCGTOAGGGTGAAGTATGGCSCCGGCíATXGTGGGCTTTGfTGCGGArCCGGCGCT

'l''VGAAAGGAAGGG'.:CGAATGGGGrGTGGGGC’AAC’TATGG rGXTCGAGAGATGGTCGCGGCOTGCAAATGGGI OOAGAACA

AGATAXGGGAGTTGGGCCSGAGACCCGXAGCGTGTCACOGGCXTTGGCGAGtCCGGCGGCGvGAGCGGGCyCGGACTGCTG

CTGAGCTCGCvGCICäGGGAGAGCGCCTTGääTCAQGCGÄTäCTGOAXAGgCCGGQXGTGGCGAGGGGGCGCGCCäCGGT

TÍCTGAAAGCGAAQGGAATGGGCíGGMíCTGöGXGCGaATATXTOOGGTCX'AGGGCOTGCGGAtGGÖGGCG&amp;ATTGXCGA

AGArCGGGCAAÏGGGGXÂTACCCAXGtAGCGCCXGTfCACCAXGCCGCGGCGGATGGGïGCGXXTCXOGAGGGCGXTGTT

GGGGGG&amp;GGCTtGACGTOGAXGGGTTGGGCAAGGGGGGCfTCCGCAA(G^TAGCCGGGCTGGTGGGGGGAAAG:GGGGAGGA

AGGGCGCGCGTTTAGGGATCGCCTGÍXXXiTOA/GG/OGC/f CXGGX'XXTAÍCGAGCOTA7OTGACAGAAC.G/AXGGGG'fGACG

AGGC/A G/GG/3GT/XGG/GC/:: /GX GXlX-/./'/:/GG:A:G':X///A.CGGGGXCG/'/././.C:G/XXG.XX.://./://CCGGG///:'/'/.'GGGG.XT A/X:GAGT//.'AA.CAGCG/A:X.,.GG://GCrGOTGG/./:/://G.:/X/r'rCGOGXXA/ //.ΧΑΧ/:///Α/.,/:(./ΧΓΤ:ΧΑ/Α://ΑΓΧ/ΧΧΧ?ΤΛΧ

GGGCATGCCäGGAGCCOGCGGXCGGGCGGGCÄGGCäXGGAGÄCGAäAXXCGCXATGXOXTCGGAAAGGTGGGGCX'GTCGT /ΧΧΧΓΑΧ-//. AXGTT/’/:/:-:..:· Λ X:::.XXA:///A/./..//..:/ /:/. //:Χ:/Χ: //../:/: Α\, A/. :..,·Χ·:/.//Χ//:/,/:Α\Λ./.::./ΑΧ//:://,/,./:/..//:/XX/Χ::.: XTG:'·/.:/,/ 1//XX/.: //,X,AG/AAA://A./X,/:A/. /.Χ/./Χ·/:/:./:/·/-//: /Λ/., A/, λ /:///./:/.//,. // Α

GAG'PTTTGGTüAGCâGGTGGGCTCXGGGîGXXGGXGTTGGAGXXXGGCGGAGGââAGCGTGCCAAGCCXGAAAAGÀGGGGG

CGGCÍTGTGCGTGCTTCAGCACGCCGTGOOOÜ'IXGQCOGCCCACGGGCíGtGCCCTCTGGCTAGAAGGAAG-XGVOtTGCG V Χ/Α/Χ/Χ/Α//: /./////A/;-’·.:·/: ' ///?/:Α\:/ΧΧΧ/χ0/Χ/Χ /: V- /A G- A : ί Α·^ν:·^:'ίΧ’Α;:\!’.Λ;ϊ!^οΑχν.Λ:ίν:ίν..ί:ΐ^'ΑΓνΑΧΑ\νΑ·1νί^Χ\ΐΑΑ v'i VWxïi1 Gv-

TTGTG/vrOGAGCAGGCGCCXXXXXXXGX?GOGCGOGGGCAACÄOTCG?CAGA^GCGOAÄTATGCGTAOGÄTOGAGGAACGT

GGiXGîG'X'GGÇGGXXGGvGGGGXGGAGrÂi’i'GGGiGG'iGAG.^ôAAî/rÂTTGGAA'rGAXGTTtGrGvGGGG^XG^AXAiVGii'jviGvGGAGGGA

OGAAOAXCTCGCGCGGGfCGTTÄXGGGCXACÄGCAeCGACGeTXTlACGXTGÄTGACGCGCXGÜGGTG/GCGTTXGaAGG

GGTCGC:TGXGGGÖCAGGGGGAGl:GlAnÁGCGMC(GGGX2GAGfCTGCGGXGGGGGvGGGAAGGGGGTTGTAAAGGCATAT /X/X:/./,XCG/:/,/./.AA,///::/.///,:///:// /:/. .X/TTXTXT/://G:/. ./, .· Λ /,///,::7/:/:/./,/: A/À IX·:/:/,/ -: //A/CX/.G/XX.:':/./:/

GCXGCGTGTGCAAXTGCGC&amp;GGCGGGGAXXCGCXGTCGXXGXGGMGCeAAGGCTGCGMA/CCTCGXGOGGAGGAXXCC

XGGCAAXXGTTGACTGGCÎGXCAAGCGGAXGGGGGCCTGCXGCGGCGXACTTCAXCOXACGGGGCACGGGAXXXGGGACT GGOäCGGTGCXCAAGAÄ.COXGXTSGAGCAÄüGOGtCGGCXCGGäGGGAGXOCCäACCGAA«GCCAXGOTGTCGGGAXCGÄ

?GGGCGAGCGOCCAAATAOOAOOOCGGCATCGTCACAGGAGTGGACTGCGTTGCOTTC'!X:GATOGTOGTCAACAAGGkOG

CC7/X;CXX::TTC7GCGATGAAGG7X!XAGATGTGTGGCCGAACX//TACGCCXÎAtGGGGÏCGCXt7a>TGGCACAO/AGCCT

GATGAGAGCÖCTT'rGAGGAGAATGGArCGGGAGGGCGÁAGAGGGGXTCATGGCGXCAG'rGTTCGvCCCCGGGGAAGCGGA cacgatggcgggtgtggccgagaaagtgggtctgaaxcgcggaagggxggaacgcagsqxságggaattgaaggccgcat

GCGXGCCCGGCGAAXGCGí/IGGGOAAGATCTCGXCGXCCTCGACXCGGXGGGAAfCGXACGAAXÍ/GGvIlICAXCXGGGOO

GGACGGA.TmTTGTGGCGGGGTTGAGGGGC:j:ATCCXGTCGGGGCCGGGAXGACGTTCAGGGATGTGGGGGXGAAGGGAGA /I///XA>/GOG/A/îG / v..i/ΓG^V.Go/;Xi/i0^vA/îG /^/íAvsGO vGíVGHí^AAAAt/. v.·/. /: / .:. .XXIX///:/X/.:/:,/..//Vl/A.//.//,/:/:/.:////:/// ttcxçggccaaggtxagcïcgcgggagtxgsaatggcgàxtggtaccgtâttoggcgggatggcgogtxgggaggoggca CGTCM'GwÄS^rrrTGM'CTCOa'fiÄA^^^ AGGTGTGCAAGGGGtGGCGÜXAxGGüaaXGSGTTCTGCGeGGTAfTTCGGGCAXTGACÜTxGGÄGCGxCÄGTTCGCCÄGC GGCGAGCTCAGGCA::cTCGCCAAix:fc?GCCACTC<AGC;?AACc::GGOXaTTACGCCTGCcAATACGCCCCTCCGCAXOA GTTGGGGJVÏ-ÂÂGCGTÎCCX:AÂGGCGCTGXCGGAGTXGCGGCT(XaXGft0C:ïiG:'GGGGÂGCGtGC:TTiXiCCX;CGseCGGGCG CCGCxOGGTä1x::öCAXGXATGGGCxCAXOAXTTCCMXaTGxGGGCGGCäTGCX1AACCGGCGTCCG:XCxGOTGGCCGCC AÍCTGGAAGGCaX1XTGCAGGTTTCGGGAXACACGCAXCGQTGCCCGGGGGCGGGGUGmOAACGTXAXTCCTmTGAGGC aÄ?GATTGCCG7:CGCGGGGÄGCÄCAfGGCJi'GGÄGrGCGe«CGSGCGGTGGCGATCSGTCfGGTTCGCTGfGCGGGGXCQG TCGGICI'gGGAÂXXAàgGXXCÎxxATCAGCACGGGGCGGTTCGTCgGGCGCxACgOgAfGCGGGGXCTÇTGCgÀlATCTC GGGGGAAGXAAGGGxvXAyaXXXL GTXÀGGAxGGOGAyAxAy?Ay--a 1'I.η \vvX'AX AAyáXAaaaXíAaaaUXU'\.yAx X.:*V..‘.:y.:·..·'·. -’ ..

CTATGÖGTTGGGAGTTGGGG:TCGCGGGCACAGCCáCGGGCACGATGGACGMCAGATGTTGGGGíGGCGGGCGCCCThGG CGCTÎTÎGAGCTTGCGîGsI'CGTGCTÂGGGAGCGGTGCAGÎGGMGGGââïGGTGGGGGGCGGGâGCGGGGTÂCTOTGGGTG ÄAGCTGGGGGGCGMGÄGGGXGCTÜGAGGGCGGGCÄGTGGT'rGGGGCGGAGGTTGCGCTGTTGGTGGTTGTGC'rGGCGÄT XGCGGCAACGGGCCxCGxCCTTTTAgCGGTGCGCAGCACGGftAGTCAXGGgGGTCGCGGGCGCCGXCOATCXCGGCGTCG TCTTGGGGTTCTTïïXGGtGAXGCCAXACÂGCaXXTxTXïXCCACGGGÂTCTTCAGGCîGaCGGCxCTGGTGCGGGATCaT GCTGAGGGGGAGGÍGxAGTAAÍGXnGríCGCGTCGÁGGGCGGGüACGGGGAAGGGTGAftGCAAGGGAACAGATGivGöÍCCGT TGCCGAXCGCAGTXGCGTOAÎGCAGX VC\:YGAGAGiXXXXXGX!AGCAACTGTCTCGAGCAAÎàTGa'ï TTCTTCGy'í'tACG GCG;7X'TATGGGGCG7AGGTTGaGGAAGCTT'rT'r:'CCGGGGGGCGA7GAGGCGGCA:rGGGTCGTGCTTTCG';''rGGCCGxCT ïïïCGCGGTGGlAÎCCGCAïGAGGiXXGAiAUGGÎAOàTTTïTCTCGGGTCvIACATCGAGCXXXrrCGGGCGÏCGGAAAGG CCTGAlXGATGACAGÍOGCGATCATGGGGGTCGGAXAXTCACCArrGGGATGACÍCOAAGCTAXGAGGGAATTÍXiATTAC GCGGAGGGGTGAGGGTGGGGGxeGGGGGACGTTÎGGAGGGTTTGGGGSCTGGAGGAGAGTGGGGîGGGGGCiCAôGGfÂG GGGGGGGÄAAXGGCGTGGGGeAÄAGGGAGAGGCGTCGTCmGCGGGTGGCAGTGTGGGAGGCAGaAGGXGGGGGTGA'rGAt CGGCGCCGCGATCGGrCTTGCGCïGCAATCAAGGGTTTGAGGGGAGCAAATGÂACGAGÏGGGGArGGiXJGGrGCCCTiGX XGAGCXXXÆGGTXGÂTtAGeGCCG!rGATAGTGGGGCTGCôGGGGGGGCTCCÇGGAAAGGAAAGCCTAXGÏCGACArG<;AG GÄGAXGGCGCÄTTGGAXCGGG(GkAGCGGTCOGCGAAÄrrvvAAGAGaGGTGGGGGGGGAGCTTGAGGGGGATGGCGÄ’?GGG GA?GGTGAX:GAGGACCACGTTTTACATGAGGACCGCGTAXAGGCGGAGAíGTGGGGAGAAAGGAGfCGGAG’rGAGCGCGG AAGAIXÎTCGTGGTGGXïACGATCATGGIX’GGGGTGGCGAÂCTIGSGGTGGCTGCSGATCGGGGGfGCÏGTGGCGGATGG'r AXCGGÍAGAACGGCGAXGGXACTGGxGGTGGCGGtCAGGG^rTCtCGCGATGGGGTGGGGGGGGAXGAGGGGGC^’CGTCSC GGCACGGACArTCGGSGGGí:GTG(XXX'TGTTGTGGTÖAC:rTTCTGGGGAGGeTTTGGAGTC:;'ArA^V,rGGöGCGCTGATCG GGAGAGíÍ:GAGGGAGATíi:A:i'GCCGCCCGGCATf!:AGÁÁCGCGXGGCGGGTGGCTGGGGT";CAG:rGTCGCGACCTCGCrGXTG GGCGGCïTOACCCCATTOGTAAGGACGGCGCTAA?GCAGGGGACGGGCAGGKAT:CCGCGCCTGCAA?CTCCGTGvO'rGr TGCGGCTTTGAXCAGGGGGGTGGGTGTGGCGGGATCGäCGCGGGXGäGCCGGCGAAXCGGGGAAGGCGCCäÖAI'AGGAGÄ AXCAGAGAAfGCGCGxGCGGCAATGAAGGGAGATXCGGGCGGGAGGGGGGCXGGGGGCAGÍGAGGGAAGAGGeGGTGGGG ACX>GGTgAAACSAGGAGGGTATfXGiTGGGGT:AAGAGATGAGAGCXXsXÁGTTGAGCGAAA?GGGÍxXAGXXGTCCXGGGGGG GTATGGTGATCCGAGAvvCGGGGGCGGGCAGGXGCGCG1x;AAíGXXÍÁGAGGAG(XXG3GAGCGGCG(xATC'í:GACCXTCAT? fTGGCGAXCATGGGCAGGGGTTGACGÁACGfTGCAGCGCGGGGGGGXAXCÍGCGTCTATGGAAGGiGAT'rTGTGTGGAGAC ATCGXXGTGQGGGATiXxAt1X;TGGGGCGACG^XTAGGGAGT?GGGGCCCTGTGGGGATCGXCGGTTGAAG.GGGGGAGAGGT TG^G ,vGC’l aGx AGCGv..'xXa^GGA:AxGy:x.vCXxGAGGGxxiG;x-GAG'GWVxvjAAÍ'ΐ'·'χνχν I A-v x xGGaΆAA? ΐ χΑ'χ\λλχΑΥΥχααα.χ ν.χχ GCGaAGATATGGTCCTCAGGGAAGGöGTCtXGCGGGGTGTTGGGAAGGGGCXTCCGGGGAGCXGGGGGGCGXXGACGGAG CCGAXGGCGGTGGGATGGGATGCCGTCGAGAGCGCGCAGGTTGAACCACATCÁCAíGCGGGGGGTGAtCGGGTGCGGACC vAxÁXAvGGTYAGAGj i'OG' vvvG. yAvGyXi GyAÁAOAG\AAi::CA:aGTX vAXxGxXxaGXAXXyAvG ΓΟ’ΑχΑ -!·G;vA: X y- CXy: -X :’Y XXy- GTGx'lXXXXXXxC Γ GG G y: A'y ΧχΧ^;χΧνΛΧ:ΧΧχΧ\ΧΧ'ίΑΑΧΧχίΧΧΧΧΛ:: wXXXX.X'VA·. y- ΧνΧχΧΧΧ:ΑΛ:^Χ.ΑΛ:ΧΧ.·ΧΧχΧΧ'ΧΧθ:Χ'. y.. GCACGCaGGGTCGGAGAAGGTGGGGGGCTGtCCÂGCXCGXIGOTGTîDGAAGTC'Â^

GCCGGGCAiGG*ï'':A;GGCATGGGiv:*GGnAGCXGCGTGCGACOGGTCXX'VGG'.DGATCGGGGG*i*TGGAGCATGCAGGAAGCCGG

GgrG::GGGCGíGíCGGMGGTGGSGG'í’GTTTíGyGG^SGTCáCGGGCMG^TCTCGGGÁGCTTGGGGGGGTQáGGáAT:i:GGCC

GGGGTGCTfCÄGMGÄTÄGGGcmöGGCGCÄCGCGÄGGGSGGTCGiG^TGGTGÄGGGGTGGGGGTGGCCTGTGGGaGGGCGG

G^veCGCGtTTiGGGsGGGGMKCG^GGGGÄGGCGGCCSÄGGAAÄäQTGGTTGGGGGGCGfTGGCGCTGAGCGTGGGGÄTGCC

A.XG0GTGCGAÜGTGGGGCArC3'DlAAA0AAGGCG::AXTTGACGAGGTA?G*VGAAXXAXXAXXGÍATTCX'::XG.GXlXGCGGAX GCAGGGGGGÄÄACÄTGCCAAAATSTGAAGTGGAGGAGTGCGTCGGGGTTCTGÄGTGTGGGGTtTGCCÄTGGTGATGGCTC:

GGSGGGGCGGmGAA&amp;GAGTAGAfGGAGTTCG^GATAAfCCGfGCTCGCGACGTGGGG'rTGGTTGAGATA’rTTCAG^CGTG

IGAGACCAGGCGTGeGGCGGGGAlGGGGÄ'rGGGeAGGAGTXGGGICAGCTGGGTCGAGTTGCÄGG'rGAGCXACGTAAGAG agaí^cagggggtgggcgáagtscgcgggggtaggatcgí:aí;G:Atcgtgtgcíagcgggcgg SEQ. ID Go G >gocj IG G ( ï'kGD;

G TG.AA.AA:AaAGAO ΑΧΑ:.'AAXGG'GGGO’GXAAX'-AXA::0 Ov v-'OOOGPODGV A A-oA-AA’....‘..;:::'00OO aaa.-aA: axaA vAooo'TIoAOG>A ΑΑΧΧΧΑ..O í Ο.:'.'::'::..’:..'.!:. :...\::0C'ΑΧ A; A A’X..A'Ο. A.· .: .:.::.::.:...:.:::...A.AAAA:...A A· ,:. :....A.:..A.:'.A.A..:A. O'G^'COOOl·· ' .·: Α.:;'·'.:Α.Α.

Ά Gl'Al AA:. J.IGVÍ'XA AA-A-GTC: A.::.::..:.::.A.:AA..A:-AA::.::·.:::,.·:,::: A. A A A A.A... aAA\:A AAAAAA... A .:. :. G GTaAAaA'AAA:A..?T A aAAAAA

GGGCCGGGCGGGGAC'GTGCGAïGGCGGCGGCCGGGÏCGGGGGAGGGCGîGGSGGGGCâCCAGGGAGGGCCGCCGCTIIGC

:A..AAÀA A: -A A.AA ΑΑ:Α AÀX Α:.ΑΑ,ΑΛ:Α::Α GG AA AA AA ΑΑΑαΑ αΑΑ ΑΑ::·Α. Λ GÇCOAGAoGÀaCGAAO.GOÀ ΑΑ,.Α· ^’L-I'o rG-: :'0/GDi:A TCIGGTGTGGíAGlAGAGGIAÍGGGAÁGGGGGGGCSGCGGGTGAÍGATefGGGTIIÁCGOGGGGGGGTTTAGGGGCGG'rGG'r GGCGCGGGGCGATAGTAGCGGAAA:GGTGCGC1AGGGCAG;GG;G3CGtGGGGGICGTGAGGGTGíGG:TAGCGGGGGGGí5AT ICIGGGCTTTGTTGGGGATC';GGe?5CTl,TCAAAGGAJGG;iCCGAATGGGG'rGTCGGGGAAGTA'ÍGG?rG;r:rCTCGA5::AT{A: aC'GaaoXv:: à ?CCAGîGv1 vOGGTTC*OOoA:C AGvvGvX AAoOvô^AiATGG^GGOAGG^Av^v^v'G^Oî'VO v'^ 4 O À :.0^00000.-^01 00:0000 Goo OCoOOOOO.GAOoO OAÁGGCooAGí. OGAA-O: a\::a:XICgCCoGX:vGOAoAoXo:a:00 AA G-X1GGOOOOOAa1G.-A oCoAXOAGO· ΌΟΟΑ: GAvOoO: AOGOGGGGT ο·Ον’ν.Ον·\Α..:.;. A -;- -- O.Á.ÓÁ'-.GXnO:AGGAaG'AA AáaA...A.AaAA:. A .A A A :: A'A·..'. ..'o-A.ΛΑ·.G GÁGA::'...*':' Α-:Γ^ίν GGCGGGCCGATGCGGGGGAATTGAGGAAQATGGGGGAAGí'GGCAGAGAGGGAGGTCGCGCGAGIGGíGICAAGCCGCGGCGG: ArGÍÍGTCGGATTGGGGAGGGGIATGT?GTGGGGAGGGTGGACGTeAmi:GGeGTGGíXAAGGGGGCCTTCCGCAAGA:GAGG GGTGGTGGICGGvGGAÄGGGCCGACGAmGGGCXGGGTTTTACGGATCÖCGIGCCGGTCAAÄAGGGGCCTTGAA'GA'GGGAG CCTATGGCAGAGAAG^AATTTGGIGXCGAGGGGGAGGCäTGGGAGCGIIGI^GAGGCGGCGAAG-GGCGAGGCCGACGT^IGGG GGGGvCGTGGCGAGGG?TTTföGGGAIÄGTGAGTGCÄACAAAGGGAICGAGGtGCT€GGGGGAGGGTTCGCGAAA'GGGGG AAGGCCGGfl;TGG:iXAGATCGCIIIAGGG(GXGTCCAGGAGGCGGCGGTC(XCCGGCX'ACGGATGGAGAG(XAAGGGGCT A^XTCGTCGCäÄAGGGGGGGCCG'GGGGGGGTATCTATGTTTGGGTC'GGGCGAÄGGGGGCGGGGGGGGGI'GGGAGAGGAäA CIÍGCGA:CCGGGGXGlGAAK:GGCAXíG:SíXGAGCIGC:GGGGIGeA£GG3GTGGCCGATCAGGGGACGA&amp;AGGGCAGIGGGG OCGC T A:'.XXA aa GO..AGGga:GAAa'A.aA a.A'A A Γ ΓΤΑΑ'ΑΓ A:G aoa: α:':;ααΧΧΑΧ.α αα.:'ΑΑΟα,:Τ:Α0'Γα:Α-α.ΑΑαΧ.ACCaaGaA:.

AÄGGCIGCG&amp;ACCGTGAAAAGAG 1GQ ID Go 4 >Sso GD 18 ( foiiilí A.O A::.'.A A-.OA:: a/G... .AAA.: AA.-AÁAG'AGaA. A. A G-.AO: A'OA.GG· GvAGOGG-OGG GOO · : A I"'. I'.:^A. G:· À :. :AA AAA::' .. A::... 0..0 ' AT:... AAA..AA:: v A.· O\. A...'.....AvAAV A A·. A.·. Α.AA v.. .: .0. Ον: :..:: A '.:'G ‘.A A:·:A.. Av.-A: AA’· λ Λ '-:::ΑArÁO-OA-A-ΟΑνΟ::

AGCAGvGaXATGXCGACGATATGGGCGCGÍAIXGGGGAAAIITGCTCGGTGAGCGGCAAÍCCGAAATGQAAGCCGCG GCTGATGCGCAGGGAÍÁ'TCIGGGCGA.CAGGAGGACaAXGCGGTCGíAXATCATGGaGAACGTG:XCGAÁGGCTTT(A;

GGGCATGRTGCGíítATGCiEiGPriYiGGGGATCATGTGGAAARATGGCAGCGATGGEAccTGAAGíFRRArGGTTyTiG CGCGTGCGCAIAOGOGGCSeAAAAGCAIATTATGCGCCAAAGGCGCTTRICAIGGCGCTTIRGlCGIGGAAGÁGTCCG CATACIGGCGGGAITGTCGCTTCGGATCGGGICFíATGICGCAIATIAIACCIAÍAACGAGGGCCAAAGCTTRIGGGA CGCGTTCAAGGCGGRCGAIGGGGáÍAtTGCGGCTíIíIÍTTTGCCACAGOTTIGCGACACRAAGTATTIGAOGACCAÖS OCGTGGCCCAGGTTGACITTGGGGCGCACGSCTCFAAARTGIIAlGAGGAGAGGGGIGGGCTTCTGGTCGIiaAGGRI GTGCGCGGAGGTTTCCGGGTGGCGGGGGATŸGCAGCTGGAOGCAÎTTGGGyAÏCGAACCGGAyCTOAGIIGCTGGGG AAAAXGCIGTGCGAATGGGGAlCCGATCICCG CGCTGGTSGGCTCSAACAASGCGGGC GATGGGGC GCGGSAIATAT íGÁTGACGGGCGCCGIGíTGGI'TCTGTGGGGTACGGATGGvGGCOGCGAfCGAGGCCGTGÁGGATCGGÍCGftGAGGCG CYTYATCTCyAAACIFRGAIGGCCAGCGGCQCCGCO'LTGCGCGCAOGCCIGOAGGCACAGTCTCAGCGCCATGGTCI TGAGIIGARCCAGACGGGGCGGGCGCÄGAIGITGCARRIATTCfTTGGGGACCAICCCGAT'IGGRGGATCGGCTAIG CG'rGGGGCGGGGCGGAvÇTGAÂGGGCGGGGGGTÂTGGrGAGCCCTA'rCÂGÂÀTATGTÎXCTÇïGGGGGGCCGA'AAC^A GITGACGATGTAACGGAGACCCTCGAGGeGACGGATOGCGCGTTCAGGCGGGTCCTGAGAGATTTTGeGieTCTCCA GGGtCATGGCÁTGTÍ&amp;AGGGAAGTGGCCGGAGGGGGA FπETROP S«qw<a«osssi ; GAG IG As 3 >Eeq IG G {FGæD; VKGHGGAGGEAGFAÄFATGLARISISRGASAI AEIPMLGÄTÄI FGAAG IR GPKΑΙΡΕΤΟΙ GAV PCVGGDv KT FEG IG FAAPPvGDl PERP PA P PPIGsAGT RDGPE PAPOC I OLAP EGRGG RAAGTS GGCL.YLGIG3 FKGGGHGGG PARI GG Y GGG FGGGS GAI P ¥ IDG 3 ALAQKGV?VV T FEYRAGI LGREAP PALSKPSPGGVSGLYGL LOME AA PKVP/ylEG REFGGDPG LYTA FGGS AGASAEGL GIF: 3 RAFF SAFEQAI LQ F F GI.ARP LAI LS PF FAAG FFLGADI GAL RRADAGE :..TKTAQSR:í PASPGPTKFRPMG'/: GUGVGLPT LLVDAFAKGAEPKIPVEVGGNAOFGRAP TDPLPVKTVI.AYFäYLIEQFGDFAGAGGSCAPMSDAGVPAäVARLFGOSGFGFGIGLFS AAFAPRPEPARRY RFIGI PGAGPRPATRGGEIPYVPAELG PGGVGH FGGLEGGRGAFDTK SAIPRGAAFxPAVxYHGVP&amp;vCTLSHLf PFSRRGY iFTrGGGVGFGSGLGFSPSKAGCG'GF: SPG XD Go 5 >Soq IO : 3 > Fus.! }

GAAG TRQRD LRBRAS RVIPSGAYGSPSfREEP PPFFQP RAPAAGAR!GOAPFOPYI DEMO AYGPLGEGY'RGSPIIAAAOAGRLLGOTGTGPSEIPAGLAEAPFGMVRSADGAFFCKHGSD AFGIAMVLAPARTGPPillLGAKGAYPRGiRFWLTLLTAGIL.AG&amp;RVSTAYYIYADAvGLSL AFFAGDGDlAAV!YsTPPRHFVFFDQAAAQEEFARTAEECCPPTCIALFvV DDVPASRRVAR OCGRTHEGI PPaLRCAGKCSkAGYPIGAELGSLKARDAARDT riIGSFRF.SAVPGAAAIR ILRi:iRFTPYIFIIAASGAGLRAG:LEÄGSQRRGEELSDIGFAGRPQIFPADQPDFPlGYA PÄAAGLFGGVYIFPYRNMPLSAAHTVDDFIEILEÄTDRAPFAyERDRAGEQRRRIEEOLA

To sotyo those oL|epA, m «dtHtke of this type A dsaractorfeeii i&amp; that it certains ah 0?>iyt«e «Aich shows at seqyssee;isl®nt?iyt8 thoSIQ i.D No3 tSeq 10 9 (hnsD)) as weil 88^1^¾ is adtlMon, a eösubstaie for at the used enzyme, an eioynie of the SEQ ID No 5 (Seq ID I 9 yFumi >) a^wtaMMSte,

With saefemacklltiv^ Mfe-hiy anti; epsggMoly dqpsding for m tho » mats*- rMlfe: possible noi tse one hand.; whereby ft«·.;:§peb|iÎë:..*^m?;pôdoeed. in this procedure catalyse the dSpsWot* ôîMmrôûmmêfaMmîMm:wvàm>MMym4%$®à$m path, mû «ft:the other tad, degrading myo>to\msj..% esæmpJe duribiáe:^ tatsíSli ioríthOípsödaelms ofafcdfcoi er a|M durius the preds^tipsv o* food dis-eeîK during the production pfnegss,

Suehnn addiîiV« wbiéti <:psstííins tttï ewsïymt? olf the Sif|lB1dí> 3$Sbq:iD. 9 asweiias optionally, in uodstson, a çosnbsJmtc for she used enzyme,$fr.enzyroe of the SFQ ID No 5 (Seq ID 19 (hamu} aszd an inert «errièr, excels by selectively deeding» and henee detnxiiyisa htmomsins Ihe use of an additive accorditi| In the i»· dost;. whÉh gsseritiäliy ednslsts of isolated tenzpneras wellss, opiloisaUy, their enSubstrates and cáfHots* oSersdhe advantage ihat the former will keep sh# catalytic activities is no etiviKnuoehf add under conditions in «btintb lor \nrss.M vmjdote Kncs^gamsms would hot orhardlyée active, whig, tMÄtisq aUowiftgitetópifiestó^ highes sjxrclfsc activities and Use c3taly2^sm«^eflBel#ÉôtiOMm (hr#^iêiKhb: öfuadesiíetóíáb reactions,. tn addition, problems caused aeeotdmg the prior mM'M0m\kixd by the use of reproducible germs will be safely avoided, and additives merely eontnlhittg Isolated e«Kys«es will, moreover,

Ml:. l&amp;Âiâidi*· Sfii&amp;ötte for a selective and sentroM aetjyaflM,i<o., for snstancëv is à pattieoiar site of &amp; digestive tract, as well as the avoidance of an ùstdesifëdv ëiôvsstçil «dÖÄ^tio«. öf increase the specs Itty. the additive is smprov&amp;d· is a way so isst mengysne mo4lfíiÍ%imbieq«tep»^ló methods, mutagenesis or molecular évolution is usêdy if an enzyme is used, which shows at least it? 3¾ sequence identity to eat enzyme with the SEQ ID No 3 (Seq ID 9 (FomPjh it is possible io degrade further snycoiohän; io adbiíon tó tiw proteblÿ degsndeaMe Ämontslds safely and rehuhly mdependent.ftmx. oxyg«$vÄre% m exlnststve^ dotoxlicadop of the ibmönlshis presoot os: tor csanspie raw vegetable products, cas be achieved.

By designing tie additive Is a manner thaflhe esgyptés are used sheathed with a projective eoafisg, as ist cor-respondent« with a presetted Hsrshsr developtnelst of fhedsVeistios), if wsl) be safeguarded ÜsatrlhbÄ-xymc m Iggsf M 9« Identical enzysnes will he secured agsirsst: any prestanutc loss of activity so as to safely pod reliably do-· veiqprihslf aCfiosit in the intended site, tor instance àndhs-pstroiotesîmai tract.

By ipspllrghty further developing the addífivo ht a -manner that the enzyrue is a cadsozylesterase of the SEf): ID No 3 (Seq: ilD 9 (htnsD)5 the enzynns qoailfiod itr substrate catabolism will he subsnmtiidly applied so as to vsssure, sn addition to a reduced-amount ofthrepx$ms to he applied, that no undesired side reaqtioïs will occur svhest oip|; said eoaypsc,: lit eorzespdudciteo with a further development of Ihsi-iuvenilon, the additive: is Uscitiri an oxvp<. odödepenslcsd essviosspetsf: during the production of hioed;.aóol, afeiig witlt n mash .or a s^gesahlp staritpg m^om\ b\ sslocting the aihliilve: sttyh tlsat the: çnzyoies eontaiswd tlsefeirs are completely derived lidfo feiscierin eat&amp;lping the eataholtssst of: ismonisins via u highly spécifié depadatfosi path, it is feasible to use the same with high specincity. acfivíity tistd efiëléncy so es to enable the additwo to fee abp used ioobnologically so an oxygen -independent envmontSÄ, fd^íthm.:iíéferí^f«^:-FradiitWgcçqf||^.tô the invention is u^ed ibr the ovygesi^mdepndsos; or anaes'obic t-vassnent of a vsigetahie starsissg materiid or a ssiash in the productum s>f bioetbaaoL bs dús ease, it is feasible to saMy aftáMfehly-fe^^^-mptgost^ èomained In tbc vegot#1e stts*ihg matériái or raw nsatedal harsn-less during the production of hioeihanoS in ars osygess-independenf enviroome-st so ;ss to subsequently aiiow for the ms of residue i'Ohvethanal proáuctíöSí,; rsatociythe .fomao8:.örAíéő ÂâSSè,::4th«f';ÉM% # ffltër I?yl8| a# ::pllëi®hsg without farther pmcmmg and, la partiou lag .«Éfcpti Slestioh as aihed tbst is Ire« ; of ihmötisi Os..

Irt the following, ike inWohlob will be explained : in more detail by way or sxemsiaty ;eroptl.wne«is-®mi Figures. Therein:

Fig, 1 depicts îhe isosîîsÂ-æsiaos]k gene cluster:

Fig, '2 illustrates the MkFaelis^Msatso/OîgyalorFmhdnwin caiixsxyigsterase FosíFí:;

Flgí:;Fshows:SL<kpM3iíO!5 purve nFliybföly®ed Euoonlam Β·:

Flg> 4 Illustrates the conversion of ihthsmtsin FBI ijaUr: h^drö|vs«;<if fumsïïlîsin î after dre addition of bar·" kwyks-enwe SEQ ID No 3 (Seq ID 0 ( FurnD)}; and

Fig. Fillhwirates the degradation of hydrolysed íuraonisín H.FB$%y :ffeç;:iaidïiion:':ôî'Mj^ôMn^Éf^a:':§BQ. ID No 5 (Seq i.D lifFumi)},

Fig. ! depicts a mmpnisin-eahtboiic gene cluster as « partial ssoneooe of 1M2ö base pairs oliüteÉM strain having the accession number DSM )62114. in the /Wa-gene cluster of the prokaryotic strata DSM 16254. the transcription of ihe open reading frame is controlled by a bidirectional promoter located between ./Ew/I sad /has/. Thmeluster encodes proteins involved itt the regulation of she gene expression. like e.g. faro® and FouiC, in the recognition of the substrate and its kansport:, likee.g. Ftrtoi, EuroA ássd FntofE sori ta the eatsShsitsm of a substrate, like cog. ForaD, F«mE„ FurnF, FnorBvFniniand FitmK,

Examples

Example 1 The esteyme ionéin'·« of futnowwo catboxxiesterave

The furnD gone (SEQ TO No 2). which sncodes a Eomatism e:nrtjoxylostorase.: was akarati and expressed in Ptchia pastorb using stpadsAl protedmes. Fite ins-tagged enzyme was: recovered and porlSed irons the sttpethtpaftt -.««ÉÍiure··fite, enxynte eoaeeatrafioa was determined and the snsysnapinatic p&amp;í&amp;oraters wete defermkigd wish seven ditierent substrat* concentrations tanging Irmn SO pg t© 35 m| FB; per lire and an enzyme concentration öiö.35 og-'mi. The roaeilorte wem buSSmed mWroM Trls-EFbulfc ípEI.Ö) with AU sng/ml bovine tarom albumát and iaeabated ai I0°C-Samples were taken aÉer (I, 3¾ 6¾ 4M and 24(1 minâtes of incubation and analysed by HPU.'-M&amp;MS. Fomoutaio B* (FÎT) and hydrolysed ilamaMn mm tpartdfted, based on a calibration with She purified reference substances and a oeanpMoly iniefriaFFBostaadard.

Fig. 2 illustrates the Miehaeiis-Meatcn crave for tbe%idmlf«ts-offoHmnM»%;^B.s) % iunorastn eserboxy-iesterase FurnD, which was determined at an enzyme coneentratino of 0.33 ng/roi in Tris-Ci buffer (pH 8.0), with initiai: enzyme speeds h&amp;vlng been plotted againsi the xobsleaW concentrations, ctirvg: shows a drop at: higher sttbstrafo coocc-ttrations, sirtco the enzynte speed was calculated based on the product, t.e. the forma·· Ion of hydrolysed FB·. Since hydrolysed lvB( is formed tora FB; in a two-step teaetkm via partially hydrolysed PB; with hot one tde&amp;rbalylic acid tdde chair· which was rdairtsd and a side chain which Was cleaved, the fonna;bn of the end prodttet was delayed: at higit sobsöWe cooccatratioas. The Mlchaelis-Mcatea coastom KM whs caic-uiated as 03)0 umoi/l, witleh was eqalvaietti to 650 ppb, arsd tlte ooovsrston rate was 900 per second.

From Fig. 2 restdtS: that lumnaikim can be rapidly arid cotppteïely hydrölysttd wÉ the barbOjiylFskrase hr the reiev.un conceuu&amp;non rattge« iExapipie 2: The cahtlyfic aetivify elsMFBl (hydrolysed iumonikm 8¾ ^htoirt^l^Ä :¾¾ :i) ,'j^o 4 was çlopéd psing standard prpoetiares and expressed irt E- ediFunder She control df :a baihdi'iopttage lE pfotpbter, l'hç haeicrkd colls were collectsd, resuspestded ilu 50 nth! sodium plipsfjhate bnifer a.».d .t»#f tu^ai^n'ip: ^&amp;>n,. i i;, droiysed funna as in was added, rand the samples were meubalcd at 2;1,;C. Samples were taken at tiro« intervals söé analysed by ÜPLC-MS/MS. Mo reduction of the hydrolysed P8:: concentration was observed. When à eosnb-gprme su# as, fer iKstsmce, aandisfe acid like j>m^c-^¾-ër O^iâçeistg was addedfe iitss reaction, ths corn» pfete degradation of the hydsolyscd femonkln fe 2-ketö'-MFBi coató be observed as illustrated in Fig. 3. This sub-stance is totally harmless for maminals.

Example 3: Enzyme activity in fee mlesifeH cnvmonrnem

To ammim %· mxgmuk d«si% ^sabetsd swioe pits sym:u$6i::^ismnspi^ä'teähSb tinder uxygeu-«xclosù«f:«ad.vxmiimâ.'!»·:*» anaerobic sterile bench. Ap proxirnsfeiv Ifecm-long pieces #ämä§;S«#W4Munmft wm. seets-ed sM etfeout, %mmbm If* ififtifed id A final concentration of sbmh: TO ppm m » Wifefrgseied by needles and n;kecf w|h ;|ntest|gä| cobhmt&amp; Ade? Isis, S p;g ismdaÄ-'iöt%b^l^«ase· ht m.speets* sdhiffen, orshe same: volume ef water üb tue negative controls, : respectively * was·iojeOfed Μφί^ψο(-&amp;$$Λζ Igeatâgii séctiôtïs wese tnehfeatsd a? 38¾. S»“ plea were drawn by the aid of ttçedles and anahsed bj HPi.C*MS'MS. It was shewn that, at the time ol tbc titst: sampling siet two hoats. famonisfeli háti ídfeédy hson completely hydrolysed in the duodenum add jtpnatn. ixarople 4: Öeferminídlgn öl the tepipemtnre mngepf the Activity of fombökht carboxylesterasè To ïdætsrmhsé -Ûk 'fet&amp;pM«.rd Ä$P irt which femonisiu carlmxyiesterass la active, l.b nghnl PIJAI-:mtèmx$tesîmis<&amp; id 20 mM TrHOÎ buffer. pH 7.0. wax kcubstedAsiife 0.i mg/mi BSÄ and 10ppm futnoakin Bl at difiire«? ten^drätures. It was shown that the temperature opiimsjp ' forÉt®· enxyme;: ·#&amp; ;30*C. Enzymatic activity was sill: ciesriy éSiÉÜtit and; even 5iFC> FUMwathosyidaihraaeIs, thus, sÂbié^,^pflMÂ'ïmàir the- ternpstvtare ebhditidns prevälihg is the digestive », or ht fee course of process step la tie ppxlitçtkio of feods: and feeds, which take piacé at elevated tempetaturos.

Example 5: Determination of the pH nn^epftfee smilvliv of femonisirs earlhsapestefasg To determine the pH range sa whutt mmookla carisoxyiesterass is active, Teorell -Stenhsgeu buffer was used, This buffer can be adfesfed Oyer a ränge of'W pH tsnhs wibtha saure buffer capaelty by tire cbiilhnatfesi sf dtfofoi/phosphofo :ÜfM«tó^ítyfe$^pe:*»m inchbafed k this buffer with id ppm fehfppism SI &amp;t different pH vaines and 25¾. at ayonceairáilöU öfB J agimfdTbe highost: aPvity was shown at pHB.O. yet activity cetdd be doferofe^CiäÄÄölb Äfe ;frgsH 1iO?':Tbls-rfv%-'-wiidtfeÄs broad pfifange bas enabfec fee ieehnokjgieai appheatio« ofi.be ensyme äs a feed addiiive or hi the course of bxxi and feed processing.

Example 6: Feeding test with piglets

The test sens performed in a test »iahte wiih i| stalk fer 10 animals each. The stable was equipped wife a slatted Boor, pan troughs ami a côh^ufapciXiWolled feeding systerti. The automats were arranged aiong the kail walk, Bvety day, the stable climate was antmaaiicaily recorded, and the temperature was set accordiag tn the standard recounrtu'miatloris for piglet breeding.

For ibis test, 120 muted-sex weaned pigs fegc: about 4: webks, average setting:vvelpiíí 8,21 kg) word náód> lach piciéi was earmarked anti hidividually weiglred. The 12S piglets wets randomly distrrbntod atnorig IT stalls, Ail piglets came from ibe Austria» Breeding Progrtun SHTB (pparge white· x landrace) x Pietfeii), immedlaiely upon weaning, the piglets svere Mi# a starter feedd&amp;r iwn days,Äer this «dliupht period the : ehangudver to the test feed took place Feeding was effected I« two phases: Weamag pasa days: 1 --Mi hreodifsg phase days 15 - 4.1. The test feed was mixed indivkluaHy per stall viable spotmk fesÄi-iSÄilÄ» fe dry ferfeitWidb n buy as h tusistfed of the namfeor df piglets, weight development sad feed enhbnmptioh. Wafer was nvaliabls: nd libitum. The 12 stabs were divided Info fear different applicsiflnn groups at; if tree repetdions siteh nha receive;! ihe following tidnrnstures in the abtrye-described feed;

Respiratory problems were observed io the positiv« control with almost hälfet' the amo'ads. even uns dropout occurred. All other groups appeared healthy.

Performance data

Example 7: Enzymatic degradation ©f fomonisifts-in bitxd:har>oI oussk

Sitipples of com ro&amp;sb bio the pteucsioa of bioethasol wer« taken surd mcbbsstedai 30 Ôte degradiftion of temntsin BI Pavmg Peen investigate after the Mdftioo of ??£> utwis of csrboxytestsipso SBQ IP No 3 (Seq ID 9 (FnotPi per cubie tRster of mask under sftnxoifsfeir^:dtne in minutes). Samples were inactivated by boibog-up after having been taken and subsequently centrifuged for analysis, an<l art aliquot of the supernatant was evaporated rite residue was taken up in 200 pi sasnple tefe «oofaiutOf C'^iabefted irtfcroai ftnoooisin standard, shaken ibr i.S min, centrifuged off nod then sssbjected so tC--Mtenahte. Front this results that as Illustrated In Fig. 4, futnonisin FBI :1s cotnpietdy converted ituo hydrolysed fumornsin HP8S. After the addition of amino·· trans if rose SEQ If); fid :¾ die hydrolysedftsníonlsin MFBI is eompleteiy degraded«? barmless eon^etttpns: as ibiss·· toted in Fig. 5.

Raampie 8; Degradation of fantomsins and their derivatives in cort) tortilla mush and eornfiake mash

The aotivfty of ft?® Rsmonidn'-dsgradftig ep^tnts was examined tn corn mush sa-rtpics (corn grits) for She production of corn tortillas and coruftakes, Famonisitt'-conOtmlnaied corn (abo-rt 1 ppm) was ground to corn Hour, mixed with water and boiled op. For the production of torn data the cot a mask cooled to about; SO to 09¾ was sup-pieirtenlsd widr a mixture ofpmteittases in alkalit?« srsiaftem After Aftern the pi t fiad fallen below a, preier#iy boiow S, a mixture of oarbaxylasiataso tntd a-rtivsotorÄrasö fSOO --:1()60 U/m3 each) was added and tnctibatsd for further 30 to 60 mitt.: Eonceotiftg:ftta: pt'orloctron ofeoreíltÉeSí a fiont mnsb of ground eon? and barley malt was boiled up in a pressure vesse! Í^iáfeöÖi©^ifeö«í).aftor't5Q.©íi»i|:ts.'b«fov»f 60*C (preferably 50X). an en«y-made mi attire comprising; :esrboxyf«st«r»se«ad arateramfbras«: ;pö0 -:|Ä; ϋΛπ*1 each) ^at. added and incubated for further 30 to 60 mim Samples;overt? then: drawn test this mixture sttdexamiocd for FB i and HFBI residues as in. Example 7. HFB1 levels:-were below SO gpb lit nil of she samples, the Ht· Bt formed of FBI apparently bad bees? condmsonslv futifer reocied. The:measured/valuer for FBI are indicated in me Table below, Tábla: Bnkyrtsk degrsásbon ofFBI and MFB ï le eannessslt fomsniisie »ocsölratíön in ppb fpg/kg)

SBQ0BBC0 BtSTlbG <110> Erber Ok r :: an gesellaera il

<120:> beset estei: f für den enryarstsschen ebben von Mykotoxinen sowie Verve neuro nemeiben 013 Ob PÖSS33EP <150r EBl3000331.7 <l5i> 2ii03--G3-->XS <1600 3 <1306 PatentIn vers : on 3.6 <210> 1 <21:1> 15420

<2 L 2 > EGA <2X3:> Epbinyopyxls Sb. <22Q> <221> de ; ÏD 1 {fne} <2220 {1} , , US420-) <330 > <JC3> F,1426263 <303> 2003--06-11 <313> :1)..(13420} <4Gö> 1 t g key g egst: ergtsaeott otscggtggk sctcgttcgc: ecseakesta estcacsgsy 62 rtngggattt ccsaefeg&amp;sc gggteeeggo ctgccggccc acatt.fccccg gssegensta 120 tyyg:ygatt:t gyseaateog gtteosgggg eager egga g ygcggygt: :: k eancgeggg :: 130 gyggagaygl: gyatntggtc ttytcostga asggtttttg gee 1 gcaggg arsaagyaos 240 m:aagrrga·: geegggaegt tattgegaog aagggaaecs gttegtggsy r.gccgke:acg 300 actecaggoa geengt: i: ::gc cgtacoggga cesggattsg tgaosatego ggogacctgt 360 eeqgtygkofc tgtaaakgyg ckcggggaka tayqecgagq cgygetagtg eaggaeeggg 420 aggascssts rooeettgte ttogsgcgca gogaggaggy gatccangtc nggcgacatg 460 agycegaaga eatennggae gygttcgeeg gocaaaoatc gtgccaatss ttctnegcee 040 gtgaggogca tgaogateto oagtacgeaa yytgagtgee yycgkggcyg yaeakraget 600 gtggttagkt gakgegekga teggegaace yagtgsÇkyy agttggatgg ceggaeekka 660 ccstgtcgag cekaackar g aga reeggaa aeggaeceag anattaasat agggycegsg 720 eq:gá4:eatag gca gage :: gg togggctqga aacaátgctg qqgtcgttvg tcqotátagg 7 00 aga a :: :.: Leg g togascaaat tattqe.óags isgaaaeaoe tgetgettet goaeaaaa gc 840 eqcgatgtag saggK.aáatq: 4·::·;;cg::eget gtaecagsea geeggágcgt gg4:4:eaaa.tt: 000 egaategacq acctaaaeat toteggeatt gaaeacegat: oátgegatga agegetgetg: 080 a;c::gag;::.ago gecca-atagt eggteogaga. tcgagoctgg aagí tagcég acea4.4.1 ' gg 1020 agtotaeggt tgtocgagcq aacgga ::ggg cgec:ga:;;acq gtegogatgc g a ::aggcaga 1080 gq::atggtgc g 4 t.geaageg g&amp;egeagaet gaacgOgeeg cogooggegg ggagtgagtá 1140 alaggeateg cagtcaa:: 4 ·.: qqq:cgqetta. ctggacagaa qggttgag&amp;t: tgogaageg:: 1200 aactgtgaág g::gaeg:: ::-::g gal4a; cage '.a;:gagg4eg aagaagaagg tgtttectqe 1,200 átcgaaegcq teg;:ctttqet gatgqggaag gaqgaaatcg stcqegaec;: 4 caacttaca 1320 eaeatagega tegacagaae. actgaaaeco aggeoagaag geggggcgta geaaeoagge 1380 gaatgaaagçj aegtaçqaet ; tnasgageg eaaatí'egag ttgacggagg ::aaagaaecg 1440 cg4:ctgaaoa geatoteage aataaat::ga attgagegte geatgaegge aggggtcgaa 1000 tagetagaeq aggett.ggce agegga- aie ::egqqaacgg gtggcgagga acetgagga·:: 1500: gtegategge asa4:at4,et:a ogcccagcti: a cage a4gt: t aeteaacegg aetggotgta 1620 a;:aggca4 a4: aggsaggcga cgi: :: 4 a:agt 1 aageaaace4 cGcagcgegg tgOcctteag 16B0 aatcgggaeg cegatttaga eaaaacettc c;:;:gatqaa a lagattceco aqaqgggsqg 1840 tgqgttgtag agattgaage atccaggaeg acatgeetge gaogccggqg açcoegtgqt 1800 tcqggtqatc gaggtegtcg: ectqogâtat agagt:aggtt tactgaaggg acttsteett I860 gegatat iiag a4 acaagegg aqaergagac egggeacgcg gcgqaggaea gqot s tegae 1020 gaggteggag gaaatcOOga qtooagacac atategetea agcààaqgat gagegsegee 1380 a :: aa g cage g acetagiega 4:ggaagacga gc::aggcqa g ectgl geaga agaga ;: ·: gag 2:04 0 eggeaegceà tai:tgqtcga ggaaggeaag le::·:gaacgg eagaegetafc ::aaccgaggg 2100 atcqcqqaca geategaegg aggcqtagag attgeggttg atggtgagat tgettteseg 21.60 aagetegagq tgeqtaaqga aaaaggaggc agaceaateg agittcaage caatgoeaat: 2220 gtctgceago aagecggaag sgecgagqta gacanttgeq aaattetega lrvagaccaa 2280 gggaasgteg gtetgaggagg gaeagacaac galeaaagaa tgggaatttc tgtaçabggg 2340 agtcoegágt ggagaeggaa, ege agg cg :: 4 ateoaggaag ateaggaaat ao acgaqcc 2400 aaggogatgo gatattaega a;: gaaoceag gttggtgtgg qaax.aagcat agg:, gaa ele 24 80 ageataeacc :: gaaca g tg 8 eggseaaate aiatgeggag cal a g gaa eg agttgtagcg 2620 a age tga :: ::: c ggqqogaage cgatafcteae gage g g 4 a ca tcgeagaeoc te:.qqaacqa 2 OSD egagetagts aaattacegt agtegaaggi; sgetaggaea ac:.a.::gagea aaaacgcgax 264 0 g a ::.4.4: :: a..:::.·;; a gggaaggaag tga ca ago a a gcegtaqqat ccgagaqaae agagaatate 2100 gggaaagaca gtgaaxieatg tegtagogaa gqgaâeggga tattggeagg aggcoatgte 2160 gaaceagcgg egageegtta attcatagge aeggatteag taatgtagaa ea4.a :: aga a 2820 gct:gac:qqgv aagtgaa&amp;oe gategtagge aaaagoagtg aagaaggaga 4aaaaaagoo 2880 g a aggreggé ago ·: r.gcc'gc gggti:gaaac aacegsatgg ageteggeee tga tgcaïtfcc: 2040 cagatctteg 4.agegaaege agtigstgaa gccegaaoeg gegtgggaaa egfeagoegge 3000 agacqcgecg coagteeaga cetegscocg attgaaeaaa gee::.gaggaa cjcaagt tgat 3060 ategategag eatgtgaggt ;:ggtegege.a gaaaaggttg eeattcogaa ggaegaggti: 0120 ceggtt egae eagaggaegq g-::a4 at ::gee eaggttatgá Cagétgatae ;a:.gt.r aagog 218Í5 S.ytsSsgtaKaiá. gyg ttggayy tes hcáavc;: gSasssg: ssgaas: áagyqeísgísst: ggStaagy s gs: 3244? gtegyssaagq s eggs: agyag atgssateatt gaaetgssteg et: « get. segg atgassaaegg 3300

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X x g ax:qgeagecg xgxrixKOXelgg xqaxiocxggxgx gxx:teggt:tc 10080 x x gxgax x:ga gx:atgx;geag xxgxggxiatge qx:gx:cggxxx eagt:egtx::xc axxxcge-xaxx 1018:0 tgegfcaegat geaxxxxrxxg. eeex-tgtege egétgaeegg tgxxOxateeg gqggax:qaat 7,0200 xggxiaxgxs tetbgtcegtï gxx.aeggggg :x|xqx.accgx x:qaagaacxx: qçgx-ggexxxx; 17:280 t:Xatxsesgxaa cax:ea.cxx|ae gx;t:aî:7:ggxx: i:eatgax:gcg xxXgcggixgtxs egltXéX:aqe 90320 •x.x: é.cgcxx x; x a gagea xqex g ag xîxtategc gaaccaasgc aixtxXOrxstt ggxxxgeggqa XQ7180 agxiegofct:qt. ·:;χχχ:.χο<:χχχΧ xasxgxxxxrgq xxaxaaegx?x:t: agxxx;x x:x; x; attc.teta.tg 710440 a;: Xexgaggt ggsx:gagat:e aax:x:t,teagx^ aagg.egtxegt. g·-Xiqegccxg xxaax:t:gegga 10500 gccgggxKsXt ccx.tg x x:gaa ;g!:egaaqeea agge^tgeexu;: egeetcgXee x;gagga x x x:x: 10680 aggcaaälet tgsatgggta açaagcgeat gggggectga tgeggogsse ttesacgtâc '10 620 ggggeaegae -a tat gags et: ggcaoggtgc teaagaitcel gtiggageaa ggcgtagcct 10600 eggtgggaga aaeaaaeoaa tgeeatgctg tggegstog« tggcegagog cccaa&amp;taag 10740 aaggcggast egtaacaegs ctggaatqcq fctccattctc gstagtogta sagaaggagg 10800 eattgegctt tttaogaigag gqcgaagatg tgtggcegaa gegttacgae atatggggte 10860 gettggtgga aaagcagaat gatcagatag ctltcageat aategatagg eaggcagsag 10020 aectettteat gcagfcesgtg ttececcaeg tgeaagaggs aaegataggg ggtgtgggeg 10980 agaaactagg tetgastoea gtaéeeatgg aaagcaOgga ggeegaatte aaegeageat 11040 gagtgeecgg egaattegge ggacsagato tegaegaect ecaaacagag ggaatagaac 11100 caaagaaâta eaactqggce aggeggattâ ítgtgceeac gtteagegoe tateatetee 11160 qqcacqqqat: aacettaaee tatetoggeg tGaaggtagá aagecgtgeg egget catoa 11220 tggagaeagg tgegccgaaa asatOtectgt t ·:gcttcgqg ggaaataatg aegggeag 11280 tteteggcca agg at a ; at ·:: get eg ai ta: g gaatggcgat tggtaççgla eteggeeges 11340 ecgegggttg ggaggeogca egteatgoag gait: at gate tagtaaaaat: gat gtctgae 114 00 rtqcagecgg egeeggaget ggaageeagg eg cat ta tag agatgagcaa egeq ·: gaege 114 60 aat tgegaag gat :: at geqc ggfeatttect: geaatgaact tgeagegteo 11 tcaecaga 11520 ggaga t at ca geoaeetege eaafeatatge caetegtgoe a&amp;ggttgeta i. taagcct g a 11580 aaatacgcea ctaageaaga gtasggaata ssogttceaa aggagetgta ggagttgggg 11040 etegagagat -segagtage·:· taattgqeec eggaaggteg oogatcaet a tcgeaaaaat 11700 gegctcaaaa :: t tees l a 11 gtcggeggaa tgeataaaaq g cat cet t a t gettgaagee 11760 a :: et t case g ggqatgeaet tttegegaaa eaggeatgag tgaeeggcgg agggttttae 11020 aaagteatte cfctatcsgga gatgattgee a tegeggega aaaeaat ter t.ta11 aegeg 11680 atggegatgg gggtaagtgt cat toga· tt tageggaega t.cgg>- at.ggg a a t1 a a get' i 11840 t:tt. ta tcaqa aegt g oca at tattcgggeg at.aeg tga ·: g aggegactet gegatatate 18000 ggcgecageg aaggegaggg gtgtsaaqaa gcqgaeqaga cattttcgac gaaceggaga 12060 aaattteate cagceattgc at at.gge11 a ggactttgtt tegcggeaac cgccaagggc 12120 aeoaaetacg a tea t ate 11 aggetggcag yegecct a U: e gat: 1tt eag attgcaggta 12180 at cataggga aeat tgggqg gateggaatg gaaqtgggcg agateggect cet a taget a 12240 aagctggeeg gegasgsagt teetagat:ca coggcaatge ctgggccqga egtttqgaetg 12800 ttggtgetta tgcttgaaat: agaggeaaag agccaao: ca etttaeeqgt cegoageace 12350 gaagtaatag gegtegegat egecqtccat etegqcgtcg tettggeatt ctfctctggtg 12420 atgcaataaa gcaaetttg·: aaaegqtate tfceaggat.es eggetetagt gcgeqafceat 12480 geteseegeg aggeasgtaa tgaettegee tccsgocctc eaeegaaa>sa gggfctasaea 12540 atggaaaata tgaagtecgt fccgegatege agtsgcgtea tgeagatcgt gagagtggag 12600 agtggasact gtategagaa atatgatlta ttegtttaeg gettatatga eg tata tett 12660 gaga g a agat ttttteagac eggagat ta a gaeaaafcega teatgetsta at t ggcaa-.:t 12720 tttggcgetg gttteateat gaggccattg agggcgaott ttatcgqgta atacatagat 127SO egagteggge at eg as a agg eetqa t agtg aegategaga taatggaagt agqaa-::ac :: a 12640 acaattgaga tgaatvéaag et a t gaggea &amp;ttggat.tae tageaaaggt tstagtgctc 12800 gtcgggegaa t:ttt:geaggg tttttceget ggageagagt agggtagogt etaagtgtae 12060 11qgcggaaa ttacqtagee eaaatcgqga gget t ::ti ca eetagtggaa gtatgaaage 13020 eagc&amp;ggeqg aegtcatgat cg a ax; cagey accggtetstq egotgc-sete aacgct::tea 130¾¾ egggagoeaa agaasgactq gagetagcég gtyceattgc tga Logget, c ottgattete 131.43 accgaga t ac qytqgatgeg cégétét: cto segg a a a g g a aagectstet cetet agyag 1,3100 ea eaaggego at t aga a egg eg a at ecet a eqcgaet.tgc aaca gage·; g ggggatgatc 13200 atgacgggea Lggaga 1 geo gat cet. eset aag&amp;ccacca ti taaatgst taccgectst 13320 gcgceg&amp;eáe tlggcgagaa agcaoteggá atgag aeeqe a a g a; g tavi, getggttaca 13300 atcatgqOcg gcgtgtqgaa cLtcctgegg ctLccyetcg gggqegctx:;; atcqgat.oot. 13440 atoggtagaa coeegatcat acaggtegeg aoggtoaacg ttctcgecat egectttcec 13300 ctgatgagot gyetegtege ege&amp;ccgaca tcoggagcec ttgeagetgt teagetgoa13500 a·:,:;;tcaracal getttagaet atatáatggg gcgct:eat:cg agayaçtxlaç cgagáetaeg 13020 aaaeccgcca ;;tagaaacet tagotaeteg afcggcgttea gteccegcgsc ctegcagl te 13630 coeggca tea aacoattgga aagtacgqeg cteatcoaag cgacgggaag caattccycg 13140 catgcaatc:: qgctctgttt agaggea 11 a atcagoa tag agg let ege egoo t.cgaaa 13300 agqctgagca ggecaatcgc ogaaggcgec agatagg&amp;ca atoagagaat getagt gagg 13060 caatgasgcg agata eggyé gg·aggtgcg ctggcggoaa t· agagaaga ggcgttgcgg 13920 sc;ggc:tgaaa egetg·:: agat atgaatggga c.aagacatga gagcagtagt t.t.aceyaaa·: 13080 ggagcacttg tcatgggggc atal.get gat; cegaí.acccg eeyec.ggooa ggtgatcgta 1404 0 a aga ea aga a eatgeygca·: atgeggatet gaectteatt t.t. a g aga t ca tgcgaaqgtg 14100 tttaagaacc t tgcat egere ggcgggl a ac gactetatgg aagttgafctt gt gtegagao 14160 at cg t tetgg ggcatgaa at etgtggcgág attatagagt teggyecete t gagea tag t 14220 cyettcaasc ecggaeaget; tytgtgcaeg at gcagetgg egatagestec gacoggagcg 14 200 egg&amp;cgstcg getaat:eggt tgagtatcaa ggegggetcg gegaatatat yytectyaeg 14 34 0 gaagegetot tgetgeetgt: teegaaegge ettaaggaga aaegegegga gttgaeggag 14400 aagatggOgg t cgqqs:ggca tgeeglag&amp;g a teg cg cage ttcaaaeoaa teacatcött 14 400 gage tea teg ggtgeggaee ggtcgggttg gaagtagteg atqccccgaa aeataagasa 14520 gttgctaagá ttattgegte ygcleca tee a a cg at age; c ctg etet t ge; aa.agcggo tg 14530 ggcgccqaog exes 11 g s; a ga t eagagegáa gaatcaecot ategecs.gge eg&amp;ge; agate 14 640 gaacgeaegg t. eg g tea agg aggggccctg teeagctaat tgatglaaaa gteroaaacg 14.700 atattagaat qeqt agggea caegggoct g ctfccggeatg agatggacgg cgeqcccgec 14760 gg g a ceo;; g a aatggt.cg:: tggegaatgc st.gcagaagg aegagatega gegaatgata 14820 aggat: g 11 ta aagsgateaa g et ce a at t c tegegasett aea aggg a aa gga&amp;ttcgcc 14330 ccggtgat.te aeatgatagg tgagggegaa ct.cgacgtat etaegeteg·; taccgatgtg 14940 attggactgt cegatctaee gtccgegttt gàggetatae ggagtaaagg ageeeaagea 13000 aaagfcgattg tggaeeattg gcgcaqagcc tgaqggtgcc aagggtgega ag :: t gage«! 1,3000 egtcaasie-aa ggegaegttg acccggtat.g tgaaeatccc aa ta ttot t a egeagetgaa 15120 geegt tggt.a aacetgccca aa t a t gaacs: g tea y tat tea gtegqggt t a teattgaggg 15186 gtltgesatt gtcet.eqct a geaccccjgeg aaaeega: s:c g a t gt act te egatàêtaeg 15240 tgatotegag etggccaaee ttaatatctt teaggaeeta t:eogaeaatg agtgcgaaga 15300 aga t oqqq et eggeaggegt tqat. teást t:t. gggtagagtt eaagttgaté t ; a g ta a g eg 1J360 agaacsectc etcegataee tgegcegagg tectategça gg>al:çgtete qqgcgt.yegt: 16420 <210> 2 <2X1> :. £23 < 212:> OK'ä <213> Sph ; oeoopgai a sa. --:220:-- <22 1 > Dec ID δ Ç2o:MD <222> (1 j ,, ; 1.223} < 3 0 Ο > <300 > F0426269 <30 9> 2009-06-11 < 32 3> {:(}>.:{ 16 2 3} <: 2 0 0 > 2 gtgaaagago aooaatgcog tggeqqccgg gegtcccacg cl/jcgcccac cacgOggett £0 gegcggstaa geettOOoeg qqgggccOcc gecategcefc eggeettcat qettggegea 120 aOtgccaDtc eo-gtggctge gcsaaetgac gateageago: togttegOca lacecagtcg 180 ggoyscogteg aqggcgtegg gcgcgaegto gagacttlist tgggsa:tacc ettegcaec;: 23 0 ccgecgqtcg gegacctgeg atggegaeeq «ggco cage egagegcat g gqegggca ec 300 aqggaeggcc gccgetttgc gcccaao;:go: ateggg&amp;acg ageggettag agaagggage 360 cgggctgccg gg;::cgagoga agac 2 g ce·: a: tstctgaafea tefcggtctcc caateagate 220 ggtaaggggq ggc2saac;gt catgaOcteg: gtfctscggag gtgggtttag eggcgg:;tet 480

yycqoqgtge estattatga eggofeatgeg co egegeaga agegeatgat ggtcgte&amp;cg 04 C tteaactafcc: gegeegggat ooogggct oo ct tgeecate eggegettte aaaggaaaqo 600

Gggaátqgpg ogteqggcaa gfcatggtctt ctegaPótge tcgsggogtt. egaatgggtt 660 eacaacaaeo: naagggagi :. eggeggagae ccgaaoeoeoq tcacggtcnà tggcgagOcc 320 goo-ggegega gcqegetegg act.get acte acc·: ege ego t eaglgagag egccttcaat 380 gaggcgatac tgeaaae::ec gqgtcOggcc aggcegeo.eg caacgcattc ::gaaaqcgaa 840 cogaa tegye 2. g g age 8 qgg agaegatatt astgctctac ggcgtgöcga tgeeggeyaa 000 ttgaggaaga tegegoaate gcgaataeco atqteqcy-oo aytteaeca-s geegceyecg 960 gtgggteag* pfcctggaegg a·: et. at to 4 g egaeecetté ceq :: ege ::qc cecagcoaag 1020 gqggccotoc g·.: aagataca egt ggto ggcggaa eg aegaegaagg g ege get tt t: 1080 aeggategee Ogee eg tea g aaegy: cctt: qaatatcg&amp;g eet&amp;teteae acaaeaatt, o 1140 ggtqaegagg eg ga ege a :: g ggagegttgt tatoeegega aetcegaege ceacqtcccc 1200 geegsegtty ceeytatett tggggatagt. eagt :: caeca aeggyatcaa getgooo:Leg 1260 geageetteg oga-safcggcg aaegeegett tggagatate eetooaegag o;atfceeagga 1320 gexgqecgte accc.::.gc<>::e go:atcaagao· gaaattceet stgtottege gaatetgggg 1380 eegtOgeceg os éclatai.:; tgggoeeetc gaaggeggee cegggaeqtc ggacateaaa 1440 •:0.Ug-ceaccq aaatyteeec ugeoa: gggtg agettegegg tgcacgggyt ceeegateag 1200 agcaoqaa at cgeacogg·.:..: gegettegag cggegagggg age oca ogee ttttggttçg 1060 eaggo oy-get efcggggasgg iettagagOt tegeogageq acaco·: gees acegLeaaag. 1620 tag 1623 <210> 3 <2 lie 54 0

<2H.> FFT <31,3 > Sp hi ny e n yx 1 4? sp. <22 9> <221 > Sea IS: 2 IregD· <222> Up,, (540; < 3 0 S <

<30S> FS4262 IS <309> 2002-66-II <31 So (1)-,( 540; <4302 3

Val Lyo Sih His Sis Cyp See Qly SI y /'a a Hie Syr Fro- Ale Ala Fro 1 5 10 15

Ala Tar Try Sea Ale Arg lie Ser Val Far Arg Sly Ala Ser Ale He 20 25 30

Hie Try fhr Fee Mat Les Giy Ala Thr Ala He Fro Val Hie A : a Sin 33 40 43

The Asp Asp Fro T,ys Leu Val Arg Hie TOr Sió Ser Sly Ala Val Ole 50 55 60

Oly Val Ole Spy Arp 151 Ole Thr PLe lias Sly lie Pro She Ala Ala 65 73 75 80

Pro Fro Val Oly Asp Lee Arg Arp Arg Fro Fro Ala Fro Fro Arg Als 85 80 95

Arp Hi a Oly Ihr Arg Asp Oly Arg Arg FLé Ala Fra Asp Cys i;e Qly 100 105 HO

Are Sie Arg Lee Arg öle Qly Ser Arg Ala Ala Oly Thr Sei Sie Asp 115 120 125

Cys Lea Tyr Lee Ass Ils Tí:g Ser ?; o Lys Sin vai Oly Lys Oly 0 i.y 130 135 140

Lee Pro Vgl Mot Ha Try Val Tyr Oly Qiy Oly F he Sor (Ily (Ily Ser 145 153 155 100

Qly Alá val Pro Tyr Tyr Asp Oly Ser Ala Lae Alá Sir Ays Oly val 165 2.70 173 val Val Val Tur Ohe Assis Tyr Ara AT® Tl y lia Leu Oily Pus Leu Als 165: iM 1.96

Als fris A 1.3 Leu S T T Lys Alls Ser Pro Asu. Oly Val Ser Gly Ase T y y 130 505 205 G!y Léa 555 Asp Oit LeO Al® Alá The Lys. Trp Val Gin Asa Asc lia 21D 21s 220

Arg O .U: Oh* Gly Gly Arc Pro Asa Arg Val Thr Vu 1 Oh* Oly Glu Sep 223 230 235 240 A la Gly Al a 2 e;: Aie Lgu Gly Lev Leu Le a Îhr 0 e r Pré Leu Ser Glu 215 200 255

Se;: Ala Ohs; Aar; ills Al e: I ; e Lee Gin Ser Or* Gly Les; Al® Arg Pro 255 GS 3 270

Leu Ala Tax: Le ; s Ser Oly Ser Glu Ale Ash oly Ose GlO Lei; Gly Al® 275 285 265

Asp Ile Ser Ais; Leu Arg Arg Als Are Ale Gly Glu Leu The Lys Ile 230 235 300

Ala Glu Gar Arg Xla Ore Met Ser Arg Gla She Ihr Lys Pro Arg Pro 553 515 BIS 320:

Mer Gly Pro Ile Leu As g oly Tyr Vei leer Arg Thr Leu Asp Tal Asp 523 335 533

Ala Ohe Ala Lya Gly Als The Arg Lys Ile Pro Vsl Leu Val Gly Gly 340 315 355

Asa Ala Äse Glu Gly Arg Ala Ohe ihr Asp Arg Leu Pre Val Lys Thr 355 360 365

Val Leu Glu Tyr Arg Ais Tyr Leu Thr Glu Glu The Gly Asp Glu Ala 370 375 385

Mp Ilia Trp Glu Grq Oye Tyr Pro Ria Aon Sor Gag Gl« Gsp Val Pro

335 8:90 331 4 SO

Gl«: G l « Val Gl« Arg Lee pha Gly .Gap Gor Gl a rise Gro Grn Gly Ils 403 4 ló 415

Girr Leu Lau Gar Ala Ära Pro Gis Lys Trp G.rg Thr Pro Gar; Trp Arg 41:0 4 3 5 410

Tyr Arg Pha Ihr Gly Hs Pro Gly G la «ly Gr.Arg Pro Ala Tar His 435 440 445

Gly Gap GO: Ils Prs Tys Val Prs Gis Ars Ga·:: Gly Pro Ser' Gar Val 4.50 4 55 4 60

Gs r- Gat PhS Gl y Gor Gay Gis Gl y «ly Ala Gl y Gl«. Sér Gap Ha Lys 465 47Q 475 480

Leu GXa Tar Glu Vst Gar Als Ale Trp Val Ser Pha Ala Val Gis Gly 465 4 SO 4 9 5

Val Pro Aep Glr: Gly TLr Gys Ser Gis Trp Pro Arg Pha Glu. Are Arg 500 SOS 6X0

Gly Gl a Ile Gat TLr Phe Gly Ssr 01 n Val Gly Sr. r Gly Glu Gly Leu. 3X5 530 60S

Gly vei Ser Pro Ser Lya Ale Gys Gin Pro Sau l.ys 530 535 540 <2.105" 4 < 12 00

< 512 > DLA <213> Spfei.rsaopyxls ara <22 Orral 1> Sag ID iS {funVO r333.> U$ , , pl2S9) <30ö>

< 3 08 > P04 2 65 6 S < 300'· 2000-06-111 < 513 > fl),.:13Spi < 4 D Ο > 4 atggcgaacg gaacaayuoe gaaeystctc agagaacgcg 4 cattocgggc 60 gggatgtaog ggeacgagtc gaeaeggttg ctgccgccsg aattcacccs gctct:tcagg 120 c aa g eget. g g gggcaegaat tfcgggscgcc gsegageage cetüMega etatatgtge 180 gcgtatgggc caaatctgct cggttoccgg oaatocgaáa ::cgaagccqc ggecgatgcg 240 cagegsattc ·'oggegacac catgaocggt eetteggaga taatggtcaa cctegcegaa: 300 geetttgtgq gcatggtecg fccstgcggqt tgggcgatgt taagaaaaaa tggaagegat 360 geeacetcaa cggcgstggt te:ageqegt góccalacgg gycgaaaaao cataltatgc 420 gosaaaggög cet at.cat gg egttí.coccg tggaaoscta ogcatactgo egggattüc 480 gctcccgatc geatgeatgt egeatattat acetatsacg acgocaaaag etteteggae 540 gagttaaagg agcacgatgg egatattgeg gctgtctttg atacasáttl oogacacgaa 600 gtat·: tgagg aocaggccet cgacoagatt gqgttcgcqc geaccgotcg aaaatgttgt 660 gacgagaoeg gtgcgettee ggtegtigac gatgtgcgcg caggt.: tccg ggtggcgogc "20 gattgeagcc agaagcatta yggtategaa aocgatctca gttgctgggg aqaatgütt 180 gegaatgget ateegstete ogccctgctg ggotagaaca aggegegega tgoggegegg 840 gatatatttg teaccqgctc ettetggtte tetgeggtae ogatggegge ogcgatcgaa 900 aecet cagea toattegaga gaogccttat ctcgaaacgt: tgateaccag cggogccgoö 960 ctgcgggcag gcctggaqgc acagtctcag egccatggtc tfcgagttga® gcagacgggc 1020 ccggcgcsga tgccgoaaat attőtttgeg gacgetcccg attttegyat eggetat gee 1080 •.gggeegegg cg! geo Iga a gggeggegte tatgttoate ectatcacaá tatgattocte 1140 tctgcggççc atacagt: t ca cgatgtaaog gagaccct.cq aggegaegga togegegtte 1200 agcgcggtoc toagagattt tgagtetete cagectcatc coattttaat y ce a ü.cq ··::··.: 1260 ggtgcttga 1260

<2I0> S <2110 622 <2120 FRT <213> Scisj re ··>>:. y a cp . <220 > <221> Sag ID 10 ! Facti ; <222> {1),.(4221 <300> <30S> FO426263 <309> 2009-06-11 <313ο (1),,(4225 <400> 3

Met Ala Asa Gly St Reg Gla Lys .Mo Leu Arg Gla Reg Ma (31u arg 1 6 1(5 16

Val Ile Pro Oly Oly Mat Tyr Gly Hü GlO Hot TM: arg Lea Lea Fee 20 26 30

Pro Sip PAe Pro Gin PLe Phe: Arc; Arg Ale Loir Ply als Arc; Ik Trp 35 43 a5

Asp Ala Asp GIu Ain Pro Tyr lie Asp Tor Met Oys Ala Tyr GXy Pro

50 55 SO

Asa I,oa Loo Giy Tyr Arg GI o Par Gla 1 Xe Gig Ala Ala Ala Asp Ala 63 70 7 5 55

Gin Arg Lou Lou Sly Asp Tor Goo far GXy Pro See Glu XiO Met Val 5 5 50 3,5

Am Lou Ala GXu Ala Ohé Val Giy Sot VAX Arg Mis Ala Asp Try Ale 105 150 115

Mot phe Cys X.<ys Asa GXy Ser Asp Ala TIP: Pét rar Ala Mer Val Leu 115 120 125 AX ® Are alá Hi s The Sl y Arg Lys ihr X i.s: Leo G y s A La .Lys Giy Al® 130 1.35 150

Tyr Bra Gly A;a Ger Pro Trp As® Thr Lao Has Tirs Aia Giy lie Leu 145 150 X5S: 160

Ala Ser asp Arg Val Bis Val AXa fyr fyr far fyr As® Asp Axa Gin ILS 170 175

Sor Leu Ser Asp Λ .1 a Pas Lys A la Sis Asp Giy Asp IXe AXa A i.a Val. ISO 155 ISO

Pne Ais ihr Pro Pau Ary His Glu Val Phe Glu Asp Gig Ala Leo Ai® 135 2 Q Ci 205

Glu Lan; Glu Par; AXa Arg: Thr Ai®. Ary Lys Cys Cys Asp Glu fhr Gly 210 215 220 AXa Lau Leo Val Val Asp Asp Val Arg Ma Gly BAe Arg Vol AXa Ary

225 230 235 24Q ASp Cys Ser Trp far Kis Leu Gly XXa GXo Pro Asp Leu Ter Gy® Trp

MS 239 S SS

Giy Lys Gys? ehe ΑΧ;?; Ap?x Si y Tyr Fro IXe Fîsp ALp Les Leu. GXy Les 2 LO 3 LS 3 ?G

Ac?:x Lys A ie Sus· Asp Aïs Sis A· y Asp XL. S Ahe V·? ; GL:r GI. y ;L:-r PLe

275 SSL 22S T:qi: LL s Le?: Aie Val 2?: S? Met A.:.A Als Alá Ils Glu f'hr Lee Are Tip

ILS 29S 3 LS

Ils Arg Air y hr Are Tyr Ssii G! P GLr S,pp I. le Als Gur GXy Ale SX S 3LS 210 SIS 323

Lex? Arg Als GXy Les Gis .Al* Glx; Ssr GXL Arg Sis Gly Lexx Gis les;.; 325 330 333

Lys Gin Thr G1 y 2 :: e Aïs G · s Pet 2ï'P Gif? IXe Lhe AGP Aie Asp Asp 340 345 320

Are Asp FLe Are IXe GXy Syr Als Grp Aie Als Als. Gy s Gps Lys GXy 355 3 30 305 GI y Vel Gyy Gel Aie Pee Ayr Hi P Asn Met FL e Les 3 er Als A Le 21s 370 373 320

Giïi: Gel Asp: Asp Vei SL:?: Sis ts>· Les Gig Als; T5.r Asp Arg Aïs PAe 335 G90 393 900 ilpr AXs sel Iqe Arg Axis FLe Aïs Lee Les GXp Feus Sis? Sri; Ils Leli 4 G S 4 X 3 415

Gel: GXp Le s Als GXy AXs. 420

Claims (1)

1. I. Adalékanyag, amely alkalmas növényi »yemnyagkan vagy ««vény* nyersanyagot tattaitna;æ> keverékekéi jPu^onlyntek eoximes lebontására. ami jelien*«**«· hogy «attain« SEQ i*> \ 'K'*0 Κΐ Ό' *' {P ' ä'! s« S' „ ' q » C<uO »0ft* i* « <. >s V V ! \<* s s ii ' 1 i s X *!'*>* Λ s 1 *« h: iu sikaimaso# «tssint kosaabsatráfját, SEP IP No:S <$BQ ID NO;If &amp; N# boidô* \> V Nn\*v * sä x*t aoaiek«)" V K^gssl äOl«iiäx*vss. «s>îcv wx? 's' <'s ' ' ^ s äiäH ens intek védNXíSxAkO hevoova alkalmasod« akV 5. A? ; vagy 2 igénypont sxer inti adalékanyag* «æxal jelteaMæve» begy st» «n»m SEQ ID NOO (SEQ Il> {FnmPf sieähxs karixtxiiésstetái köNH taiasxtott. 4 Ai i-3. igénypontok egyike sasrini» adalékanyag alk&amp;im&amp;sAng mmi jelteme/v«, hogy ai: sAalöksoyag síss'O >' Ο-, n όλ s'X V ' \äsiA 0>' On-.s,i S\ sX s'<sl „'\sv A xur'i \ϊ»ΌΌ·.μ o!a 0^ <·0>Ο* a'îkalnwïSîîî X A4, igénypont «νϊμβιΙ alkalmad növény« koodoiás« anyag vagy <erh? oxigéntől fttggedotí késelését* ':bkÄ.tülflÄ«feäba^