HRP20220582T1 - Postupak otkrivanja genetskog materijala u biološkom uzorku i uređaj za njegovu implementaciju - Google Patents

Postupak otkrivanja genetskog materijala u biološkom uzorku i uređaj za njegovu implementaciju Download PDF

Info

Publication number
HRP20220582T1
HRP20220582T1 HRP20220582TT HRP20220582T HRP20220582T1 HR P20220582 T1 HRP20220582 T1 HR P20220582T1 HR P20220582T T HRP20220582T T HR P20220582TT HR P20220582 T HRP20220582 T HR P20220582T HR P20220582 T1 HRP20220582 T1 HR P20220582T1
Authority
HR
Croatia
Prior art keywords
chamber
reaction
genetic material
isolation
isolation chamber
Prior art date
Application number
HRP20220582TT
Other languages
English (en)
Inventor
Miron TOKARSKI
Henryk Waldemar ROGUSZCZAK
Tadeusz Marian DOBOSZ
Arkadiusz Piotr Dabrowski
Malgorzata MALODOBRA-MAZUR
Damian Romuald ANDRZEJEWSKI
Leszek GOLONKA
Original Assignee
Genomtec Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genomtec Sa filed Critical Genomtec Sa
Publication of HRP20220582T1 publication Critical patent/HRP20220582T1/hr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0672—Integrated piercing tool
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1861—Means for temperature control using radiation
    • B01L2300/1872—Infrared light
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/062—LED's

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Virology (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • AIDS & HIV (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Claims (16)

1. Postupak otkrivanja genetskog materijala u biološkom uzorku, naznačeno time, što uključuje sljedeće faze: a) biološki uzorak se stavlja u reakcijski uložak (6), a zatim ili prije toga se reakcijski uložak (6) stavlja u mjerni uređaj, pri čemu mjerni uređaj sadrži mjernu komoru (10) koja ima posudu u kojoj se nalazi reakcijski uložak ( 6), pri čemu reakcijski uložak (6) sadrži izolacijsku komoru (7) za izolaciju genetskog materijala, koja je povezana s reakcijskim komorama (8.1, 8.2, 8.3) kroz kanale, za umnožavanje izoliranog genetskog materijala, b) prikupljeni biološki uzorak odnese se u izolacijsku komoru (7), c) biološki materijal se izolira iz ispitivanog uzorka zagrijavanjem izolacijske komore (7), d) izolirani genetski materijal premješten je u više reakcijskih komora (8.1, 8.2, 8.3), i e) genetski materijal se umnožava zagrijavanjem reakcijskih komora (8.1, 8.2, 8.3), naznačen time da: unutar najmanje jedne od reakcijskih komora (8.1, 8.2, 8.3) nalaze se liofilizirani reagensi za amplificiranje genetskog materijala zajedno s luminescentnom bojom, koji sadrže fluorescentnu boju ili kvantne točke koje vežu genetski materijal koji treba detektirati, pri čemu se istovremeno sa stadijem amplifikacije genetskog materijala detekcija luminiscentnog signala od luminiscentnih markera je registriran, mjerni uređaj se sastoji od više grijaćih jedinica LED dioda s detektorima temperature (23) raspoređenim u osnovi nasuprot izolacijske komore (7) i/ili reakcijskih komora (8.1, 8.2, 8.3) tako da svjetlosni snopovi emitiraju navedeni broj LED dioda osvijetliti navedenu izolacijsku komoru (7) i/ili reakcijske komore (8.1, 8.2, 8.3), a zagrijavanje izolacijske komore (7) i/ili reakcijske komore (8.1, 8.2, 8.3) izvodi se kroz više grijaćih jedinica LED diode s detektorima temperature (23), a u izolacijskoj komori (7) prisutan je materijal sposoban za vezanje inhibitora reakcije pojačanja.
2. Postupak u skladu s patentnim zahtjevom 1, naznačeno time, što se biološki uzorak uzima iz sustava za uzorkovanje (1), a faza a) se izvodi punjenjem sustava za uzorkovanje (1) u reakcijski uložak (6), pri čemu se zagrijava izolacijska komora ( 7) i/ili reakcijska komora (8.1, 8.2, 8.3) sadrži elektromagnetsko zračenje valne duljine u rasponu od 350 nm do 530 nm, a grijaća jedinica LED dioda s detektorima temperature (23) sadrži optički detektor temperature (25) koji detektira elektromagnetno zračenje u rasponu valnih duljina od 4 mm do 12 mm, a pri čemu se na dnu izolacijske komore (7) i/ili reakcijske komore (8.1, 8.2, 8.3) nalazi apsorpcijski sloj koji apsorbira energiju fotona .
3. Postupak u skladu s bilo kojim od patentnih zahtjeva 1-2, naznačeno time, da se biološki uzorak uzima korištenjem kapilarnih sila za kapilaru (2) u sustavu za uzorkovanje (1) i liofiliziranih reagensa za pojačavanje genetskog materijala deoksinukleotidi, specifične sekvence prajmera, reakcija pufer, magnezijevi ioni Mg2+, po mogućnosti u obliku MgSO4, polimeraza sposobna provesti reakciju amplifikacije, po mogućnosti Bst 3.0 polimeraza pri čemu je liofilizirana fluorescentna oznaka interkalirana s otkrivenim genetskim materijalom SYBR Green.
4. Postupak u skladu s bilo kojim od patentnih zahtjeva 1-3, naznačeno time, što reakcijski uložak (6) sadrži tri reakcijske komore (8.1, 8.2, 8.3), uključujući ispitnu komoru (8.1) uključujući specifične početnice za testirani genetski materijal, a komoru za pozitivnu kontrolu (8.2) koja sadrži prajmere specifične za određeni dio genetskog materijala iz kojeg je dobiven uzorak biološkog materijala i komoru za negativnu kontrolu (8.3), koja sadrži reakcijske komponente bez početnica.
5. Postupak u skladu s patentnim zahtjevom 4, naznačeno time, što su reakcijske komore (8.1, 8.2, 8.3) u pogledu odozgo kružnice, komplementarne i u sredini međusobno povezane ventilom ili membranom (33).
6. Postupak u skladu s bilo kojim od patentnih zahtjeva 1-5, naznačeno time, da se u fazi c) izolacijska komora zagrijava na 95°C od 5 minuta min do 10 minuta, pri čemu se u fazi e) reakcijske komore (8.1, 8.2, 8.3) zagrijavaju se na 65°C od 15 minuta do 60 minuta, a faza b) se postiže pomoću prve pumpe (P1), po mogućnosti u obliku spremnika za vodu zatvorenog membranom spojenom na komoru za proizvodnju tlaka ili klip i mijeh a korak d) se postiže pomoću druge pumpe (P2), po mogućnosti u obliku šuplje komore zatvorene dijafragmom spojenom na komoru za proizvodnju tlaka i obuhvaća stupanj zagrijavanja reakcijske patrone (6) na temperature iznad 100°C, po mogućnosti kroz niz grijaćih jedinica na LED diodama s detektorima temperature (23).
7. Uređaj za detekciju genetskog materijala u biološkom uzorku, naznačeno time, da sadrži reakcijski uložak (6) i mjerni uređaj, pri čemu mjerni uređaj sadrži mjernu komoru (10) koja ima posudu u kojoj se nalazi reakcijski uložak (6), pri čemu reakcijski uložak (6) sadrži izolacijsku komoru (7) za izolaciju genetskog materijala, koja je kanalima povezana s reakcijskim komorama (8.1, 8.2, 8.3) za umnožavanje izoliranog genetskog materijala, naznačen time da: unutar barem jedne od reakcijskih komora (8.1, 8.2, 8.3) se nalaze liofilizirani reagensi za amplificiranje genetskog materijala zajedno s luminiscentnom bojom, koji sadrže fluorescentnu boju ili kvantne točke koje vežu genetski materijal koji treba detektirati, dok istovremeno sa stadijem pojačanje genetskog materijala bilježi se detekcija luminiscentnog signala iz luminiscentnih markera, mjerni uređaj se sastoji od više grijaćih jedinica LED dioda s detektorima temperature (23) raspoređenim uglavnom nasuprot izolacijske komore (7) i/ili reakcijskih komora (8.1, 8.2). , 8.3) tako da svjetlosni snopovi koji emitiraju navedeni snop LED-a osvjetljavaju navedenu izolacijsku komoru (7) i/ili reakcijske komore (8.1, 8.2, 8.3), a u izolacijskoj komori (7) je nazočan materijal koji može vezati inhibitore reakcija amplifikacije.
8. Uređaj u skladu s patentnim zahtjevom 7, naznačeno time, što uređaj sadrži odvojivi sustav za uzorkovanje (1), odvojivi sustav za uzorkovanje (1) koji se sastoji od čepa (4) i reakcijske patrone (6) koja se sastoji od posude (5) koja je pričvršćena na spomenuti utikač (4) i osigurava stabilnu i čvrstu vezu tekućine između sustava za uzorkovanje (1) i reakcijske patrone (6) i mjernog modula za kontrolu i analizu slike (16), komunikacijskog modula (17), modula napajanja (18) ) i modul zaslona (19).
9. Uređaj u skladu s bilo kojim od patentnih zahtjeva 7 do 8, naznačeno time, što jedinice za grijanje emitiraju elektromagnetsko zračenje valne duljine u rasponu od 350 nm do 530 nm, pri čemu grijaća jedinica LED dioda s detektorima temperature sadrži optički detektor temperature koji detektira elektromagnetsko zračenje u rasponu valnih duljina od 4 mm do 12 mm, pri čemu se na dnu izolacijske komore (7) i/ili reakcijske komore (8.1, 8.2, 8.3) nalazi apsorpcijski sloj koji apsorbira energiju fotona, a reakcijske komore (8.1. , 8.2, 8.3) u pogledu odozgo su kružnice, komplementarne i međusobno povezane u sredini ventilom ili dijafragmom.
10. Uređaj prema bilo kojem od zahtjeva 7 do 9, naznačeno time, što sustav za uzorkovanje (1) sadrži kapilaru (2) u koju se uzima biološki uzorak, povezanu s pumpom (P1) po mogućnosti u obliku spremnika za vodu zatvoren dijafragmom povezanom s komorom za proizvodnju tlaka ili klipom i mijehom i liofiliziranim reagensima za amplifikaciju genetskog materijala deoksinukleotidi, specifične sekvence početnica, reakcijski pufer, magnezijevi ioni Mg2+, po mogućnosti u obliku MgSO4, polimeraza sposobna provesti reakciju amplifikacije , poželjno Bst 3.0 polimeraza, pri čemu je liofilizirana fluorescentna oznaka interkalirana s otkrivenim genetskim materijalom SYBR Green.
11. Uređaj u skladu s bilo kojim od patentnih zahtjeva 7 do 10, naznačeno time, što reakcijski uložak (6) sadrži tri reakcijske komore (8.1, 8.2, 8.3), uključujući ispitnu komoru (8.1) uključujući specifične početnice za testirani genetski materijal, a komoru za pozitivnu kontrolu (8.2) koja sadrži početnike specifične za određeni dio genetskog materijala iz kojeg je dobiven uzorak biološkog materijala i komoru za negativnu kontrolu (8.3), koja sadrži reakcijske komponente bez početnica, pri čemu reakcijski uložak (6) sadrži pumpa (P2), po mogućnosti u obliku prazne komore zatvorene dijafragmom spojenom na komoru za proizvodnju tlaka, spojena na izolacijsku komoru (7) i proizvodi tlak koji uzrokuje kretanje izoliranog genetskog materijala iz izolacijske komore (7) u reakcijske komore (8.1, 8.2, 8.3).
12. Uređaj u skladu s bilo kojim od patentnih zahtjeva 7 do 11, naznačeno time, što je reakcijski uložak (6) i/ili sustav za uzorkovanje (1) izrađen od hidrofobnog polimera i potpuno je pasivni sustav, pri čemu je izolacijska komora (7) i reakcijska komora komore (8.1, 8.2, 8.3) kao i pumpa (P2) u reakcijskoj patroni (6) sadrže ventile (Z11), (Z5, Z6, Z7), (Z8, Z9, Z10), (Z3), ( Z4), po mogućnosti optičke, odnosno na ulaznim i izlaznim kanalima.
13. Uređaj u skladu s bilo kojim od patentnih zahtjeva 7 do 12, naznačeno time, da se u kanalu koji povezuje izolacijsku komoru (7) s drugom pumpom (P2) nalazi detektor tekućine (D1), po mogućnosti reflektirajući infracrveni, pri čemu se detektori tekućine (D2, D3, D4), po mogućnosti reflektirajući infracrveni, smješteni su u izlaznim kanalima iz reakcijskih komora (8.1, 8.2, 8.3), a mjerna komora (10) ima kontroliranu izotermnu temperaturu u rasponu od 4° C do 40°C, realizirano putem sustava grijanja (13), po mogućnosti u obliku Peltierovog sklopa, a sustav grijanja (13) se sastoji od spojenog ventilatora (14) i radijatora (15), te kotača za miješanje zraka ( 22) koji se nalazi u mjernoj komori (10).
14. Uređaj u skladu s bilo kojim od patentnih zahtjeva 7 do 13, naznačeno time, što je mjerna komora (10) izolirana toplinskom izolacijom (12) i mehanizmom za pozicioniranje (11) reakcijske patrone (6) i mehanizmom za podešavanje tlaka (20). ) vršenje pritiska na pumpu (P1) u reakcijskoj patroni (6) i nasuprotno postavljen senzor tlaka (21) i dodatne UV LED diode (29) koje osvjetljavaju područje detekcije.
15. Uređaj u skladu s bilo kojim od patentnih zahtjeva 7 do 14, naznačeno time, što uređaj sadrži dodatne LED diode (27) za rad detektora tekućine (D1-D4) i ventila (Z1-Z11) i na dnu izolacijske komore (7) i/ili reakcijske komore (8.1, 8.2, 8.3) postoji apsorpcijski sloj koji apsorbira energiju fotona, po mogućnosti izrađen od Cu ili Al obloženog oksidima, po mogućnosti, obojenim crnom Al2O3.
16. Uređaj u skladu s patentnim zahtjevom 11, naznačeno time, što je izolacijska komora (7) povezana s komorom (8.4), koja je dalje povezana preko ventila (Z5-Z7) s reakcijskim komorama (8.1, 8.2, 8.3).
HRP20220582TT 2016-12-21 2017-12-20 Postupak otkrivanja genetskog materijala u biološkom uzorku i uređaj za njegovu implementaciju HRP20220582T1 (hr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PL419907A PL235210B1 (pl) 2016-12-21 2016-12-21 Sposób detekcji materiału genetycznego w próbce biologicznej oraz urządzenie do jego realizacji
EP17002043.2A EP3338891B8 (en) 2016-12-21 2017-12-20 A method of detecting genetic material in a biological sample and a device for its implementation

Publications (1)

Publication Number Publication Date
HRP20220582T1 true HRP20220582T1 (hr) 2022-06-10

Family

ID=60953514

Family Applications (1)

Application Number Title Priority Date Filing Date
HRP20220582TT HRP20220582T1 (hr) 2016-12-21 2017-12-20 Postupak otkrivanja genetskog materijala u biološkom uzorku i uređaj za njegovu implementaciju

Country Status (16)

Country Link
US (2) US10781479B2 (hr)
EP (2) EP4029607A1 (hr)
JP (1) JP6961700B2 (hr)
CN (1) CN110088266A (hr)
BR (1) BR112019012501B1 (hr)
CA (1) CA2989599C (hr)
DK (1) DK3338891T3 (hr)
ES (1) ES2913092T3 (hr)
HR (1) HRP20220582T1 (hr)
HU (1) HUE058240T2 (hr)
LT (1) LT3338891T (hr)
PL (1) PL235210B1 (hr)
PT (1) PT3338891T (hr)
RS (1) RS63179B1 (hr)
SI (1) SI3338891T1 (hr)
WO (1) WO2018117882A1 (hr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4202028A1 (en) * 2020-08-11 2023-06-28 Kyorin Pharmaceutical Co., Ltd. Nucleic acid amplification device, nucleic acid amplification method, and sample solution position control method
CN115290606A (zh) * 2022-08-22 2022-11-04 凤凰医学诊断科技有限公司 一种检测装置

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6780617B2 (en) * 2000-12-29 2004-08-24 Chen & Chen, Llc Sample processing device and method
US20030138819A1 (en) * 2001-10-26 2003-07-24 Haiqing Gong Method for detecting disease
US20090061450A1 (en) 2006-03-14 2009-03-05 Micronics, Inc. System and method for diagnosis of infectious diseases
US8835157B2 (en) * 2007-04-25 2014-09-16 3M Innovative Properties Company Supported reagents, methods, and devices
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
WO2010110741A1 (en) * 2009-03-25 2010-09-30 Haiqing Gong Apparatus and method for detection of organisms
FR2950358B1 (fr) * 2009-09-18 2015-09-11 Biomerieux Sa Dispositif d'amplification d'acides nucleiques simplifie et son procede de mise en oeuvre
KR20160088958A (ko) 2010-02-23 2016-07-26 루미넥스 코포레이션 일체화된 샘플 제조, 반응 및 검출을 위한 장치 및 방법
US20110312541A1 (en) * 2010-06-17 2011-12-22 Geneasys Pty Ltd Loc for detection of hybridization of nucleic acid sequences with primer-linked linear probes
JP6262657B2 (ja) * 2011-10-11 2018-01-17 キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング 試料プロセシング方法および試料プロセシングカートリッジ
EP2962092A4 (en) * 2013-03-01 2016-08-24 Wave 80 Biosciences Inc METHODS AND SYSTEMS FOR ENHANCED MICRO-FLUIDIC TREATMENT
US10933417B2 (en) 2013-03-15 2021-03-02 Nanobiosym, Inc. Systems and methods for mobile device analysis of nucleic acids and proteins
WO2015191916A1 (en) * 2014-06-11 2015-12-17 Micronics, Inc. Microfluidic cartridges and apparatus with integrated assay controls for analysis of nucleic acids
CN106715673A (zh) * 2014-07-11 2017-05-24 先进诊疗公司 用于疾病检测的即时检测聚合酶链反应设备
KR102292998B1 (ko) * 2015-01-16 2021-08-26 더 리전트 오브 더 유니버시티 오브 캘리포니아 핵산 증폭용 led 구동 플라즈몬 가열 장치
SG10202005427PA (en) * 2015-04-24 2020-07-29 Mesa Biotech Inc Fluidic test cassette
CA3011710A1 (en) * 2016-01-20 2017-07-27 Triv Tech, Llc Point-of-care nucleic acid amplification and detection
JP7453664B2 (ja) * 2016-10-11 2024-03-21 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 統合的分子診断システム（ｉＭＤｘ）およびデング熱のための方法

Also Published As

Publication number Publication date
PL419907A1 (pl) 2018-07-02
PL235210B1 (pl) 2020-06-15
HUE058240T2 (hu) 2022-07-28
EP3338891B1 (en) 2022-02-09
RS63179B1 (sr) 2022-06-30
JP2020513762A (ja) 2020-05-21
LT3338891T (lt) 2022-05-10
US20210040549A1 (en) 2021-02-11
SI3338891T1 (sl) 2022-06-30
DK3338891T3 (da) 2022-04-25
EP3338891B8 (en) 2022-03-16
CN110088266A (zh) 2019-08-02
BR112019012501A2 (pt) 2020-04-14
CA2989599A1 (en) 2018-06-21
BR112019012501B1 (pt) 2023-01-24
ES2913092T3 (es) 2022-05-31
JP6961700B2 (ja) 2021-11-05
EP3338891A1 (en) 2018-06-27
WO2018117882A1 (en) 2018-06-28
US10781479B2 (en) 2020-09-22
PT3338891T (pt) 2022-05-16
EP4029607A1 (en) 2022-07-20
US20180187250A1 (en) 2018-07-05
US11608521B2 (en) 2023-03-21
CA2989599C (en) 2024-04-02

Similar Documents

Publication Publication Date Title
US20220042073A1 (en) Led driven plasmonic heating apparatus for nucleic acids amplification
JP7223736B2 (ja) 反応処理装置および反応処理方法
Sayad et al. A microfluidic lab-on-a-disc integrated loop mediated isothermal amplification for foodborne pathogen detection
Yamamoto et al. PDMS–glass hybrid microreactor array with embedded temperature control device. Application to cell-free protein synthesis
US8658099B2 (en) Integrated apparatus for conducting and monitoring chemical reactions
US11896977B2 (en) Method and system for temperature monitoring of a biochemical reaction vessel
HRP20220582T1 (hr) Postupak otkrivanja genetskog materijala u biološkom uzorku i uređaj za njegovu implementaciju
CN212504899U (zh) 一种全自动核酸检测分析系统
CN202898426U (zh) 一种面向空间的螺旋式微流控pcr实时荧光检测系统
JP2022066343A (ja) 反応処理容器、反応処理装置、反応処理方法および反応処理容器の使用方法
CN107619781A (zh) 一种单反应控温高通量微流控芯片核酸扩增装置
Woolf et al. Optically-controlled closable microvalves for polymeric centrifugal microfluidic devices
JP7330514B2 (ja) 反応処理装置
WO2017213586A1 (en) Rapid thermal cycling for sample analyses and processing
CN102586098B (zh) 一种面向空间的微体积单位的实时荧光pcr工作系统
JP2019198335A (ja) 反応処理装置および反応処理方法
JP6876162B2 (ja) 反応処理装置および反応処理方法
CN113667591A (zh) 一种全自动核酸检测分析系统
CN202116542U (zh) 双路温控聚合酶链式反应器与实时检测装置