HRP20200092T1 - Postupak za enzimsku hidrolizu lignoceluloznog materijala upotrebom gh61, dodavanjem kisika, te visokog sadržaja suhe tvari - Google Patents
Postupak za enzimsku hidrolizu lignoceluloznog materijala upotrebom gh61, dodavanjem kisika, te visokog sadržaja suhe tvari Download PDFInfo
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- HRP20200092T1 HRP20200092T1 HRP20200092TT HRP20200092T HRP20200092T1 HR P20200092 T1 HRP20200092 T1 HR P20200092T1 HR P20200092T T HRP20200092T T HR P20200092TT HR P20200092 T HRP20200092 T HR P20200092T HR P20200092 T1 HRP20200092 T1 HR P20200092T1
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- lignocellulosic material
- enzymatic hydrolysis
- dry matter
- enzyme
- enzymatic
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- 239000012978 lignocellulosic material Substances 0.000 title claims 14
- 238000000034 method Methods 0.000 title claims 13
- 230000007071 enzymatic hydrolysis Effects 0.000 title claims 12
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 title claims 12
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title claims 8
- 239000001301 oxygen Substances 0.000 title claims 8
- 229910052760 oxygen Inorganic materials 0.000 title claims 8
- 102000004190 Enzymes Human genes 0.000 claims 12
- 108090000790 Enzymes Proteins 0.000 claims 12
- 238000002360 preparation method Methods 0.000 claims 12
- 238000000855 fermentation Methods 0.000 claims 5
- 230000004151 fermentation Effects 0.000 claims 5
- 230000007062 hydrolysis Effects 0.000 claims 5
- 238000006460 hydrolysis reaction Methods 0.000 claims 5
- 230000002255 enzymatic effect Effects 0.000 claims 4
- 102000004169 proteins and genes Human genes 0.000 claims 4
- 108090000623 proteins and genes Proteins 0.000 claims 4
- 102000005575 Cellulases Human genes 0.000 claims 2
- 108010084185 Cellulases Proteins 0.000 claims 2
- 229920001503 Glucan Polymers 0.000 claims 2
- 239000002994 raw material Substances 0.000 claims 2
- 238000011084 recovery Methods 0.000 claims 2
- 238000005406 washing Methods 0.000 claims 2
- 241000233866 Fungi Species 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 230000002538 fungal effect Effects 0.000 claims 1
- 239000007789 gas Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 244000005700 microbiome Species 0.000 claims 1
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/10—Nitrogen as only ring hetero atom
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- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
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- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
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- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
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- C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
- C12P7/44—Polycarboxylic acids
- C12P7/48—Tricarboxylic acids, e.g. citric acid
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- C12P7/00—Preparation of oxygen-containing organic compounds
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- C12P7/54—Acetic acid
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- C12P7/56—Lactic acid
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- C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
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- Saccharide Compounds (AREA)
Claims (13)
1. Postupak za pripremu šećernog proizvoda iz lignoceluloznog materijala, koji sadrži sljedeće korake:
a) po izboru, predobradu lignoceluloznog materijala,
b) po izboru, ispiranje po izboru prethodno obrađenog lignoceluloznog materijala,
c) enzimsku hidrolizu po izboru ispranog i/ili po izboru prethodno obrađenog lignoceluloznog materijala koristeći enzimski pripravak koji sadrži najmanje dvije celulaze i pri čemu enzimski pripravak barem sadrži GH61,
d) pri čemu se koristi između 0,1 i 7,5 mg enzimskog pripravka/g glukana (na suhu tvar i enzim kao protein) ili između 0,05 i 3,0 mg enzimskog pripravka/g sirovine (na suhu tvar i enzim kao protein), i
e) po izboru, ponovno dobivanje šećernog proizvoda,
pri čemu se tijekom koraka enzimske hidrolize (c) kisik dodaje u lignocelulozni materijal, reaktor za enzimsku hidrolizu ima volumen od 1 m3 ili više i sadržaj suhe tvari u koraku hidrolize (c) iznosi 10 tež.% ili više.
2. Postupak za pripremu proizvoda fermentacije iz lignoceluloznog materijala, naznačen time što sadrži sljedeće korake:
a) po izboru, predobradu lignoceluloznog materijala,
b) po izboru, ispiranje po izboru prethodno obrađenog lignoceluloznog materijala,
c) enzimsku hidrolizu po izboru ispranog i/ili po izboru prethodno obrađenog lignoceluloznog materijala koristeći enzimski pripravak koji sadrži najmanje dvije celulaze i pri čemu enzimski pripravak barem sadrži GH61,
d) pri čemu se koristi između 0,1 i 7,5 mg enzimskog pripravka/g glukana (na suhu tvar i enzim kao protein) ili između 0,05 i 3,0 mg enzimskog pripravka/g sirovine (na suhu tvar i enzim kao protein), i
e) fermentaciju hidroliziranog lignoceluloznog materijala da se dobije proizvod fermentacije, i
f) po izboru, ponovno dobivanje proizvoda fermentacije,
pri čemu se tijekom koraka enzimske hidrolize (c) kisik dodaje u lignocelulozni materijal, reaktor za enzimsku hidrolizu ima volumen od 1 m3 ili više i sadržaj suhe tvari u koraku hidrolize (c) iznosi 10 tež.% ili više.
3. Postupak prema patentnom zahtjevu 1 ili 2, naznačen time što se kisik dodaje u obliku mjehurića.
4. Postupak prema bilo kojem od patentnih zahtjeva 1 do 3, naznačen time što se tijekom dijela vremena enzimske hidrolize, kisik dodaje u lignocelulozni materijal i tijekom dijela vremena enzimske hidrolize se dodaje manje kisika u lignocelulozni materijal u usporedbi s drugim dijelom vremena enzimske hidrolize.
5. Postupak prema patentnom zahtjevu 2, naznačen time što vrijeme enzimske hidrolize iznosi 5 do 150 sati.
6. Postupak prema bilo kojem od patentnih zahtjeva 1 do 5, naznačen time što korišteni enzimski pripravak zadržava aktivnost tijekom 30 sati ili više.
7. Postupak prema bilo kojem od patentnih zahtjeva 1 do 6, naznačen time što se hidroliza provodi pri temperaturi od 45°C ili više.
8. Postupak prema bilo kojem od patentnih zahtjeva 1 do 7, naznačen time što je enzimski pripravak dobiven iz gljivice ili enzimski pripravak sadrži gljivični enzim.
9. Postupak prema bilo kojem od patentnih zahtjeva 1 do 8, naznačen time što sadržaj suhe tvari u koraku hidrolize (c) iznosi 14 tež.% ili više.
10. Postupak prema patentnom zahtjevu 9, naznačen time što sadržaj suhe tvari u koraku hidrolize (c) iznosi 14 do 33% tež.%.
11. Postupak prema bilo kojem od patentnih zahtjeva 1 do 10, naznačen time što se enzimska hidroliza odvija u šaržnom reaktoru, punjenom šaržnom reaktoru i/ili reaktoru za neprekidno kultiviranje.
12. Postupak prema bilo kojem od patentnih zahtjeva 1 do 11, naznačen time što se kisik uvodi kao plin koji sadrži kisik.
13. Postupak prema bilo kojem od patentnih zahtjeva 2 do 12, naznačen time što se fermentacija provodi s mikroorganizmom koji može fermentirati barem jedan C5 šećer.
Applications Claiming Priority (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12191957 | 2012-11-09 | ||
| EP13174656 | 2013-07-02 | ||
| EP13176083 | 2013-07-11 | ||
| EP13176500 | 2013-07-15 | ||
| EP13184702 | 2013-09-17 | ||
| EP13184701 | 2013-09-17 | ||
| EP13789275.8A EP2917354B1 (en) | 2012-11-09 | 2013-11-07 | Process for enzymatic hydrolysis of lignocellulosic material using gh61, oxygen addition, and high dry matter content |
| PCT/EP2013/073255 WO2014072395A1 (en) | 2012-11-09 | 2013-11-07 | Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20200092T1 true HRP20200092T1 (hr) | 2020-04-03 |
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Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20240339TT HRP20240339T1 (hr) | 2012-11-09 | 2013-11-07 | Postupak enzimske hidrolize lignoceluloznog materijala i fermentacije šećera |
| HRP20191960TT HRP20191960T1 (hr) | 2012-11-09 | 2019-10-30 | Postupak za enzimatsku hidrolizu lignoceluloznog materijala dodatkom kisika |
| HRP20200092TT HRP20200092T1 (hr) | 2012-11-09 | 2020-01-21 | Postupak za enzimsku hidrolizu lignoceluloznog materijala upotrebom gh61, dodavanjem kisika, te visokog sadržaja suhe tvari |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20240339TT HRP20240339T1 (hr) | 2012-11-09 | 2013-11-07 | Postupak enzimske hidrolize lignoceluloznog materijala i fermentacije šećera |
| HRP20191960TT HRP20191960T1 (hr) | 2012-11-09 | 2019-10-30 | Postupak za enzimatsku hidrolizu lignoceluloznog materijala dodatkom kisika |
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| Country | Link |
|---|---|
| US (7) | US9982280B2 (hr) |
| EP (4) | EP3613860B1 (hr) |
| JP (3) | JP6429155B2 (hr) |
| KR (2) | KR102104152B1 (hr) |
| CN (3) | CN104769116A (hr) |
| AU (4) | AU2013343597B2 (hr) |
| BR (3) | BR112015004344A2 (hr) |
| CA (2) | CA2888333C (hr) |
| DK (3) | DK2917355T3 (hr) |
| EA (2) | EA028880B9 (hr) |
| ES (3) | ES2773953T3 (hr) |
| FI (1) | FI3613860T3 (hr) |
| HR (3) | HRP20240339T1 (hr) |
| HU (3) | HUE047092T2 (hr) |
| IN (2) | IN2015DN03084A (hr) |
| LT (1) | LT3613860T (hr) |
| MX (3) | MX360067B (hr) |
| MY (2) | MY169456A (hr) |
| PL (2) | PL3613860T3 (hr) |
| RS (1) | RS65279B1 (hr) |
| SI (1) | SI3613860T1 (hr) |
| UA (2) | UA116789C2 (hr) |
| WO (2) | WO2014072393A1 (hr) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6396306B2 (ja) | 2012-11-09 | 2018-09-26 | ディーエスエム アイピー アセッツ ビー．ブイ．Ｄｓｍ Ｉｐ Ａｓｓｅｔｓ Ｂ．Ｖ． | リグノセルロース系材料の酵素加水分解および糖類発酵のための方法 |
| DK2917355T3 (da) | 2012-11-09 | 2020-01-20 | Dsm Ip Assets Bv | Fremgangsmåde til enzymatisk hydrolyse af lignocellulosemateriale ved tilsætning af oxygen |
| CN110643653A (zh) | 2013-02-21 | 2020-01-03 | 诺维信公司 | 糖化和发酵纤维素材料的方法 |
| WO2015004098A1 (en) * | 2013-07-11 | 2015-01-15 | Dsm Ip Assets B.V. | Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars |
| EP3063285B1 (en) | 2013-11-01 | 2019-04-10 | Novozymes A/S | Methods of saccharifying and fermenting a cellulosic material |
| EP3126510A1 (en) | 2014-04-03 | 2017-02-08 | DSM IP Assets B.V. | Process and apparatus for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars |
| CA2944957C (en) | 2014-04-30 | 2022-03-29 | Dsm Ip Assets B.V. | Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars |
| WO2015175308A1 (en) * | 2014-05-13 | 2015-11-19 | Danisco Us Inc. | Improved enzymatic hydrolysis of biomass |
| US10793887B2 (en) * | 2014-08-21 | 2020-10-06 | Novozymes A/S | Process for saccharifying cellulosic material under oxygen addition |
| EP3234168A1 (en) | 2014-12-19 | 2017-10-25 | DSM IP Assets B.V. | Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars |
| CA2973302C (en) * | 2015-01-28 | 2022-10-25 | Dsm Ip Assets B.V. | Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars |
| MY182182A (en) * | 2015-01-28 | 2021-01-18 | Dsm Ip Assets Bv | Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars |
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