HRP20140079T1 - Metoda za stvaranje genskog mozaicizma - Google Patents
Metoda za stvaranje genskog mozaicizma Download PDFInfo
- Publication number
- HRP20140079T1 HRP20140079T1 HRP20140079AT HRP20140079T HRP20140079T1 HR P20140079 T1 HRP20140079 T1 HR P20140079T1 HR P20140079A T HRP20140079A T HR P20140079AT HR P20140079 T HRP20140079 T HR P20140079T HR P20140079 T1 HRP20140079 T1 HR P20140079T1
- Authority
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- Croatia
- Prior art keywords
- gene
- cell
- genes
- sequence
- markers
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title claims 35
- 238000000034 method Methods 0.000 title claims 15
- 210000004027 cell Anatomy 0.000 claims 10
- 206010068052 Mosaicism Diseases 0.000 claims 6
- 108090000765 processed proteins & peptides Proteins 0.000 claims 5
- 102000004196 processed proteins & peptides Human genes 0.000 claims 5
- 238000005215 recombination Methods 0.000 claims 5
- 230000006798 recombination Effects 0.000 claims 5
- 239000003550 marker Substances 0.000 claims 4
- 229920001184 polypeptide Polymers 0.000 claims 4
- 238000003780 insertion Methods 0.000 claims 3
- 230000037431 insertion Effects 0.000 claims 3
- 230000033616 DNA repair Effects 0.000 claims 2
- 241000235648 Pichia Species 0.000 claims 2
- 230000002950 deficient Effects 0.000 claims 2
- 230000002538 fungal effect Effects 0.000 claims 2
- 230000002068 genetic effect Effects 0.000 claims 2
- 108091033319 polynucleotide Proteins 0.000 claims 2
- 102000040430 polynucleotide Human genes 0.000 claims 2
- 239000002157 polynucleotide Substances 0.000 claims 2
- 241000228212 Aspergillus Species 0.000 claims 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims 1
- 108090000695 Cytokines Proteins 0.000 claims 1
- 102000004127 Cytokines Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 241000235649 Kluyveromyces Species 0.000 claims 1
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 1
- 241000235070 Saccharomyces Species 0.000 claims 1
- 241000235013 Yarrowia Species 0.000 claims 1
- 239000012190 activator Substances 0.000 claims 1
- 239000000427 antigen Substances 0.000 claims 1
- 108091007433 antigens Proteins 0.000 claims 1
- 102000036639 antigens Human genes 0.000 claims 1
- 210000001106 artificial yeast chromosome Anatomy 0.000 claims 1
- 230000008238 biochemical pathway Effects 0.000 claims 1
- 230000003115 biocidal effect Effects 0.000 claims 1
- 238000013377 clone selection method Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 210000003527 eukaryotic cell Anatomy 0.000 claims 1
- 239000003102 growth factor Substances 0.000 claims 1
- 238000001727 in vivo Methods 0.000 claims 1
- 230000033607 mismatch repair Effects 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 claims 1
- 238000010915 one-step procedure Methods 0.000 claims 1
- 210000001236 prokaryotic cell Anatomy 0.000 claims 1
- 230000000392 somatic effect Effects 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
- 229960005486 vaccine Drugs 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Ecology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Claims (14)
1. Metoda za stvaranje genskog mozaicizma somatskom in vivo rekombinacijom homolognog slijeda DNA, obuhvaća:
postupak u jednom koraku
transformiranje stanice deficijentne za popravak DNA s barem jednim genom A koji ima homologiju slijeda najmanje 30% i manje od 99.5% prema drugom genu za rekombinaciju koji je sastavni dio staničnog genoma ili je prikazan u okviru genske konstrukcije upotrebljavajući barem jedan gen B za rekombinaciju,
rekombiniranje navedenih gena,
stvaranje genskog mozaicizma gena na mjestu ugradnje ciljnog genoma, pri čemu navedeni barem jedan gen A ima pojedinačni krajnji ciljni slijed bilo na 5' ili 3' kraju koji se povezuje s 5' ili 3' krajem navedenog mjesta ugradnje, pri čemu se pojedinačni krajnji ciljni slijed gena A povezuje s 5' krajem mjesta ugradnje, dok gen B ima pojedinačni krajnji ciljni slijed koji se povezuje s 3' krajem, i
odabir klonova obuhvaća barem jedan intragenski mozaicizam gena.
2. Metoda u skladu s patentnim zahtjevom 1, pri čemu se u genskom mozaicizmu koristi marker odabira i klonovi se odabiru prema prisustvu markera odabira.
3. Metoda u skladu s patentnim zahtjevom 1 ili 2, pri čemu su dobiveni genski mozaicizmi nekodirajućih sljedova, djelomični geni ili geni od najmanje 3 do 20 000 parova baza.
4. Metoda u skladu s bilo kojim od patentnih zahtjeva 1 do 3, pri čemu se stanice nazivaju kotransformiranima s najmanje dvije različite varijante gena A za rekombinaciju, koji su geni A1 i A2, i neobavezno s najmanje dvije različite varijante gena B za rekombinaciju, koji su geni B1 i B2.
5. Metoda u skladu s bilo kojim od patentnih zahtjeva 1 do 4, pri čemu je barem jedan sljedeći gen C kotransformiran, koji ima slijed koji hibridizira sa slijedom gena A i/ili navedenim drugim genom zbog dobivanja sklopa navedenog sljedećeg gena C s genom A i/ili navedenim drugim genom, pri čemu jedan ili više od sklopova gena ima intragenski mozaicizam gena.
6. Metoda u skladu s bilo kojim od patentnih zahtjeva 1 do 5, pri čemu su navedeni gen A i/ili navedeni drugi gen nekodirajući sljedovi ili kodiraju za polipeptid ili dio polipeptida koji ima aktivnost.
7. Metoda u skladu s patentnim zahtjevom 5 ili 6, pri čemu su višestruki geni koji kodiraju za polipeptide biokemijskog puta rekombinirani i sklopljeni.
8. Metoda u skladu s bilo kojim od patentnih zahtjeva 1 do 7, pri čemu je stanica stanica s inaktiviranim genom za popravak DNA ili stanica s nedostatkom popravka pogrešnog sparivanja.
9. Metoda u skladu s bilo kojim od patentnih zahtjeva 1 do 8, pri čemu je stanica eukariotska stanica, poželjno stanica gljivice, sisavca ili biljke, ili prokariotska stanica.
10. Metoda u skladu s patentnim zahtjevom 9, pri čemu je stanica stanica gljivice, poželjno odabrana iz grupe koja se sastoji od Saccharomyces, Candida, Kluyveromyces, Hansenula, Szhizosaccharomyces, Yarrowia, Pichia i Aspergillus.
11. Metoda u skladu s bilo kojim od patentnih zahtjeva 1 do 10, pri čemu je korišten marker odabira, koji je odabran iz grupe koja se sastoji od markera kojeg organizam ne može sintetizirati kao hranjivo za rast, markera otpornosti na antibiotik, fluorescentnih markera, ubačenih genskih markera, markera aktivatora/vezujućih domena i dominantnih recesivnih markera i kolorimetrijskih markera.
12. Metoda u skladu s bilo kojim od patentnih zahtjeva 1 do 11, pri čemu su navedeni geni obuhvaćeni u linearnom polinukleotidu, vektoru ili umjetnom kromosomu kvasca.
13. Metoda u skladu s bilo kojim od patentnih zahtjeva 1 do 12, pri čemu su navedeni geni linearni polinukleotidi, poželjno od 300 do 20 000 bp.
14. Metoda u skladu s bilo kojim od patentnih zahtjeva 1 do 13, pri čemu su geni nekodirajući sljedovi ili kodirajuće varijante za polipeptide odabrane iz grupe koja se sastoji od enzima, protutijela ili njihovih dijelova, citokina, čimbenika rasta, antigena vakcina i peptida.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US32268010P | 2010-04-09 | 2010-04-09 | |
| EP10159515 | 2010-04-09 | ||
| PCT/EP2011/055530 WO2011124693A1 (en) | 2010-04-09 | 2011-04-08 | Method of generating gene mosaics |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20140079T1 true HRP20140079T1 (hr) | 2014-02-28 |
Family
ID=42288683
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20140079AT HRP20140079T1 (hr) | 2010-04-09 | 2014-01-27 | Metoda za stvaranje genskog mozaicizma |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US8912127B2 (hr) |
| EP (2) | EP2647710A1 (hr) |
| JP (1) | JP2013524785A (hr) |
| KR (1) | KR20130098150A (hr) |
| CN (1) | CN102933710A (hr) |
| AU (1) | AU2011237547A1 (hr) |
| BR (1) | BR112012025858A2 (hr) |
| CA (1) | CA2795246A1 (hr) |
| DK (1) | DK2478100T3 (hr) |
| ES (1) | ES2445565T3 (hr) |
| HR (1) | HRP20140079T1 (hr) |
| PL (1) | PL2478100T3 (hr) |
| PT (1) | PT2478100E (hr) |
| SG (1) | SG184470A1 (hr) |
| WO (1) | WO2011124693A1 (hr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2723860A1 (en) * | 2011-06-21 | 2014-04-30 | Eviagenics S.A. | Method of metabolic evolution |
| US20140274803A1 (en) * | 2011-10-12 | 2014-09-18 | Eviagenics S.A. | Method of generating gene mosaics in eukaryotic cells |
| EP2783000A1 (en) * | 2011-11-23 | 2014-10-01 | DSM IP Assets B.V. | Nucleic acid assembly system |
| WO2014102368A1 (en) | 2012-12-27 | 2014-07-03 | Eviagenics S.A. | Recombinant host cell for biosynthetic production |
| EP2749644B1 (en) | 2012-12-27 | 2018-08-22 | Rhodia Operations | Recombinant host cell for biosynthetic production of vanillin |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2641793B1 (fr) | 1988-12-26 | 1993-10-01 | Setratech | Procede de recombinaison in vivo de sequences d'adn presentant des mesappariements de bases |
| EP0842289B1 (en) * | 1995-07-26 | 2004-10-20 | Mixis France S.A. | Homologous recombination in mismatch repair inactivated eukaryotic cells |
| WO2003095658A1 (en) | 2002-05-07 | 2003-11-20 | Novozymes A/S | Homologous recombination into bacterium for the generation of polynucleotide libraries |
| MXPA06008670A (es) | 2004-01-30 | 2007-04-17 | Mixis France Sa | Generacion de genes recombinantes en saccharomyces cerevisiae. |
| EP1734125A1 (en) | 2005-06-16 | 2006-12-20 | Institut National De La Recherche Agronomique | Homeologous recombination in MSH2 inactivated plants or cells thereof |
-
2011
- 2011-04-08 PT PT117151969T patent/PT2478100E/pt unknown
- 2011-04-08 EP EP13173933.6A patent/EP2647710A1/en not_active Withdrawn
- 2011-04-08 DK DK11715196.9T patent/DK2478100T3/da active
- 2011-04-08 AU AU2011237547A patent/AU2011237547A1/en not_active Abandoned
- 2011-04-08 SG SG2012074274A patent/SG184470A1/en unknown
- 2011-04-08 PL PL11715196T patent/PL2478100T3/pl unknown
- 2011-04-08 BR BR112012025858A patent/BR112012025858A2/pt not_active IP Right Cessation
- 2011-04-08 ES ES11715196.9T patent/ES2445565T3/es active Active
- 2011-04-08 JP JP2013503133A patent/JP2013524785A/ja active Pending
- 2011-04-08 CN CN2011800283522A patent/CN102933710A/zh active Pending
- 2011-04-08 KR KR1020127029534A patent/KR20130098150A/ko not_active Application Discontinuation
- 2011-04-08 CA CA2795246A patent/CA2795246A1/en not_active Abandoned
- 2011-04-08 US US13/640,313 patent/US8912127B2/en not_active Expired - Fee Related
- 2011-04-08 WO PCT/EP2011/055530 patent/WO2011124693A1/en active Application Filing
- 2011-04-08 EP EP11715196.9A patent/EP2478100B1/en active Active
-
2014
- 2014-01-27 HR HRP20140079AT patent/HRP20140079T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| SG184470A1 (en) | 2012-11-29 |
| CA2795246A1 (en) | 2011-10-13 |
| WO2011124693A1 (en) | 2011-10-13 |
| PT2478100E (pt) | 2014-02-20 |
| PL2478100T3 (pl) | 2014-04-30 |
| EP2647710A1 (en) | 2013-10-09 |
| JP2013524785A (ja) | 2013-06-20 |
| CN102933710A (zh) | 2013-02-13 |
| EP2478100B1 (en) | 2013-11-13 |
| BR112012025858A2 (pt) | 2015-10-06 |
| AU2011237547A1 (en) | 2012-10-25 |
| DK2478100T3 (da) | 2014-02-17 |
| KR20130098150A (ko) | 2013-09-04 |
| ES2445565T3 (es) | 2014-03-04 |
| EP2478100A1 (en) | 2012-07-25 |
| US20130090246A1 (en) | 2013-04-11 |
| US8912127B2 (en) | 2014-12-16 |
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