BR112012025858A2 - método de geração de mosaicos de gene. - Google Patents

método de geração de mosaicos de gene.

Info

Publication number
BR112012025858A2
BR112012025858A2 BR112012025858A BR112012025858A BR112012025858A2 BR 112012025858 A2 BR112012025858 A2 BR 112012025858A2 BR 112012025858 A BR112012025858 A BR 112012025858A BR 112012025858 A BR112012025858 A BR 112012025858A BR 112012025858 A2 BR112012025858 A2 BR 112012025858A2
Authority
BR
Brazil
Prior art keywords
gene
mosaic
generation method
gene mosaic
genes
Prior art date
Application number
BR112012025858A
Other languages
English (en)
Inventor
Alejandro Luque
Rudy Pandjaitan
Original Assignee
Eviagenics S A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eviagenics S A filed Critical Eviagenics S A
Publication of BR112012025858A2 publication Critical patent/BR112012025858A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

método de geração de mosaicos de gene. a invenção refere-se a um método para a geração de um mosaico de gene por recombinação in vivo somática, compreendendo e) em um procedimento de única etapa (vii) transformação de uma célula com pelo menos um gene a tendo uma homologia de sequência de menos do que 99,5% a um outro gene a ser recombinado que é uma parte integral do genoma da célula ou é apresentada na estrutura de um construto genético, (viii) recombinação dos ditos genes, (ix) geração de um mosaico de gene dos genes em um sítio de integração de um genoma-alvo, em que o dito pelo menos um gene a tem uma única sequência-alvo de flanqueamento ou na extremidade 5' ou na extremidade 3' ancoragem na extremidade 5' ou 3' do dito sítio de integração, e f) seleção de clones compreendendo o mosaico de gene, bem como um método de produção de uma diversidade de mosaicos de gene e montagem de gene.
BR112012025858A 2010-04-09 2011-04-08 método de geração de mosaicos de gene. BR112012025858A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32268010P 2010-04-09 2010-04-09
EP10159515 2010-04-09
PCT/EP2011/055530 WO2011124693A1 (en) 2010-04-09 2011-04-08 Method of generating gene mosaics

Publications (1)

Publication Number Publication Date
BR112012025858A2 true BR112012025858A2 (pt) 2015-10-06

Family

ID=42288683

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012025858A BR112012025858A2 (pt) 2010-04-09 2011-04-08 método de geração de mosaicos de gene.

Country Status (15)

Country Link
US (1) US8912127B2 (pt)
EP (2) EP2647710A1 (pt)
JP (1) JP2013524785A (pt)
KR (1) KR20130098150A (pt)
CN (1) CN102933710A (pt)
AU (1) AU2011237547A1 (pt)
BR (1) BR112012025858A2 (pt)
CA (1) CA2795246A1 (pt)
DK (1) DK2478100T3 (pt)
ES (1) ES2445565T3 (pt)
HR (1) HRP20140079T1 (pt)
PL (1) PL2478100T3 (pt)
PT (1) PT2478100E (pt)
SG (1) SG184470A1 (pt)
WO (1) WO2011124693A1 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2723860A1 (en) * 2011-06-21 2014-04-30 Eviagenics S.A. Method of metabolic evolution
US20140274803A1 (en) * 2011-10-12 2014-09-18 Eviagenics S.A. Method of generating gene mosaics in eukaryotic cells
US20140303036A1 (en) * 2011-11-23 2014-10-09 Dsm Ip Assets B.V. Nucleic Acid Assembly System
BR112015015594B1 (pt) 2012-12-27 2022-07-05 Rhodia Operations Célula de levedura, método de produção de uma célula de levedura, uso de uma célula de levedura e método de biossíntese heteróloga de um valinoide
EP2749644B1 (en) 2012-12-27 2018-08-22 Rhodia Operations Recombinant host cell for biosynthetic production of vanillin

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2641793B1 (fr) 1988-12-26 1993-10-01 Setratech Procede de recombinaison in vivo de sequences d'adn presentant des mesappariements de bases
ATE280239T1 (de) * 1995-07-26 2004-11-15 Mixis France Sa Homologe rekombination in eukaryotischen zellen mit inaktiviertem fehlpaarungsreparatur-system
AU2003223928A1 (en) 2002-05-07 2003-11-11 Novozymes A/S Homologous recombination into bacterium for the generation of polynucleotide libraries
CN1938426B (zh) 2004-01-30 2010-08-11 麦克西斯法国股份有限公司 在酿酒酵母中生成重组基因
EP1734125A1 (en) 2005-06-16 2006-12-20 Institut National De La Recherche Agronomique Homeologous recombination in MSH2 inactivated plants or cells thereof

Also Published As

Publication number Publication date
EP2647710A1 (en) 2013-10-09
PT2478100E (pt) 2014-02-20
SG184470A1 (en) 2012-11-29
US8912127B2 (en) 2014-12-16
EP2478100B1 (en) 2013-11-13
EP2478100A1 (en) 2012-07-25
WO2011124693A1 (en) 2011-10-13
CN102933710A (zh) 2013-02-13
DK2478100T3 (da) 2014-02-17
PL2478100T3 (pl) 2014-04-30
ES2445565T3 (es) 2014-03-04
JP2013524785A (ja) 2013-06-20
AU2011237547A1 (en) 2012-10-25
HRP20140079T1 (hr) 2014-02-28
US20130090246A1 (en) 2013-04-11
KR20130098150A (ko) 2013-09-04
CA2795246A1 (en) 2011-10-13

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Legal Events

Date Code Title Description
B08F Application fees: application dismissed [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]