HRP20100532T1 - Izravno određivanje vitamina d u serumu ili plazmi - Google Patents
Izravno određivanje vitamina d u serumu ili plazmi Download PDFInfo
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- HRP20100532T1 HRP20100532T1 HR20100532T HRP20100532T HRP20100532T1 HR P20100532 T1 HRP20100532 T1 HR P20100532T1 HR 20100532 T HR20100532 T HR 20100532T HR P20100532 T HRP20100532 T HR P20100532T HR P20100532 T1 HRP20100532 T1 HR P20100532T1
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- vitamin
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- 229940046008 vitamin d Drugs 0.000 title claims abstract 22
- 210000002966 serum Anatomy 0.000 title claims abstract 8
- 229930003316 Vitamin D Natural products 0.000 claims abstract 21
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 claims abstract 21
- 235000019166 vitamin D Nutrition 0.000 claims abstract 21
- 239000011710 vitamin D Substances 0.000 claims abstract 21
- 150000003710 vitamin D derivatives Chemical class 0.000 claims abstract 21
- 239000002207 metabolite Substances 0.000 claims abstract 13
- 238000000034 method Methods 0.000 claims abstract 8
- 210000002381 plasma Anatomy 0.000 claims abstract 8
- 108010067770 Endopeptidase K Proteins 0.000 claims abstract 6
- 108010022999 Serine Proteases Proteins 0.000 claims abstract 6
- 102000012479 Serine Proteases Human genes 0.000 claims abstract 6
- 102000004506 Blood Proteins Human genes 0.000 claims abstract 3
- 108010017384 Blood Proteins Proteins 0.000 claims abstract 3
- 230000009137 competitive binding Effects 0.000 claims abstract 2
- 238000000746 purification Methods 0.000 claims abstract 2
- 239000007790 solid phase Substances 0.000 claims 7
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 claims 6
- 150000001875 compounds Chemical class 0.000 claims 5
- 238000012800 visualization Methods 0.000 claims 5
- 239000000872 buffer Substances 0.000 claims 4
- 239000003795 chemical substances by application Substances 0.000 claims 3
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims 3
- 229960004889 salicylic acid Drugs 0.000 claims 3
- GMRQFYUYWCNGIN-UHFFFAOYSA-N 1,25-Dihydroxy-vitamin D3' Natural products C1CCC2(C)C(C(CCCC(C)(C)O)C)CCC2C1=CC=C1CC(O)CC(O)C1=C GMRQFYUYWCNGIN-UHFFFAOYSA-N 0.000 claims 2
- ZGLHBRQAEXKACO-XJRQOBMKSA-N 1alpha,25-dihydroxyvitamin D2 Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@@H](\C=C\[C@H](C)C(C)(C)O)C)=C\C=C1\C[C@@H](O)C[C@H](O)C1=C ZGLHBRQAEXKACO-XJRQOBMKSA-N 0.000 claims 2
- LBLYYCQCTBFVLH-UHFFFAOYSA-N 2-Methylbenzenesulfonic acid Chemical compound CC1=CC=CC=C1S(O)(=O)=O LBLYYCQCTBFVLH-UHFFFAOYSA-N 0.000 claims 2
- JWUBBDSIWDLEOM-UHFFFAOYSA-N 25-Hydroxycholecalciferol Natural products C1CCC2(C)C(C(CCCC(C)(C)O)C)CCC2C1=CC=C1CC(O)CCC1=C JWUBBDSIWDLEOM-UHFFFAOYSA-N 0.000 claims 2
- JWUBBDSIWDLEOM-DCHLRESJSA-N 25-Hydroxyvitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@@H](CCCC(C)(C)O)C)=C/C=C1\C[C@@H](O)CCC1=C JWUBBDSIWDLEOM-DCHLRESJSA-N 0.000 claims 2
- JWUBBDSIWDLEOM-NQZHSCJISA-N 25-hydroxy-3 epi cholecalciferol Chemical compound C1([C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@@H](CCCC(C)(C)O)C)=CC=C1C[C@H](O)CCC1=C JWUBBDSIWDLEOM-NQZHSCJISA-N 0.000 claims 2
- KJKIIUAXZGLUND-ICCVIKJNSA-N 25-hydroxyvitamin D2 Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@@H](\C=C\[C@H](C)C(C)(C)O)C)=C\C=C1\C[C@@H](O)CCC1=C KJKIIUAXZGLUND-ICCVIKJNSA-N 0.000 claims 2
- 238000002965 ELISA Methods 0.000 claims 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims 2
- 239000002253 acid Substances 0.000 claims 2
- 150000007513 acids Chemical class 0.000 claims 2
- 239000012148 binding buffer Substances 0.000 claims 2
- 235000020964 calcitriol Nutrition 0.000 claims 2
- 239000011612 calcitriol Substances 0.000 claims 2
- GMRQFYUYWCNGIN-NKMMMXOESA-N calcitriol Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@@H](CCCC(C)(C)O)C)=C\C=C1\C[C@@H](O)C[C@H](O)C1=C GMRQFYUYWCNGIN-NKMMMXOESA-N 0.000 claims 2
- 238000003018 immunoassay Methods 0.000 claims 2
- PSZYNBSKGUBXEH-UHFFFAOYSA-N naphthalene-1-sulfonic acid Chemical class C1=CC=C2C(S(=O)(=O)O)=CC=CC2=C1 PSZYNBSKGUBXEH-UHFFFAOYSA-N 0.000 claims 2
- 102000004169 proteins and genes Human genes 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 claims 2
- 238000003127 radioimmunoassay Methods 0.000 claims 2
- PJVWKTKQMONHTI-UHFFFAOYSA-N warfarin Chemical compound OC=1C2=CC=CC=C2OC(=O)C=1C(CC(=O)C)C1=CC=CC=C1 PJVWKTKQMONHTI-UHFFFAOYSA-N 0.000 claims 2
- 229960005080 warfarin Drugs 0.000 claims 2
- 102000050760 Vitamin D-binding protein Human genes 0.000 claims 1
- 108700021020 Vitamin D-binding protein Proteins 0.000 claims 1
- 238000012875 competitive assay Methods 0.000 claims 1
- 239000003398 denaturant Substances 0.000 claims 1
- 238000004925 denaturation Methods 0.000 claims 1
- 230000036425 denaturation Effects 0.000 claims 1
- 238000007865 diluting Methods 0.000 claims 1
- 239000013024 dilution buffer Substances 0.000 claims 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 claims 1
- 230000000367 exoproteolytic effect Effects 0.000 claims 1
- 239000003112 inhibitor Substances 0.000 claims 1
- 238000004020 luminiscence type Methods 0.000 claims 1
- 239000010413 mother solution Substances 0.000 claims 1
- 238000000159 protein binding assay Methods 0.000 claims 1
- 230000017854 proteolysis Effects 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 230000029087 digestion Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/82—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving vitamins or their receptors
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Postupak kvantitativnog određivanja metabolita vitamina D izravno u krvnoj plazmi ili serumu, bez potrebe za prethodnim pročišćavanjem metabolita vitamina D, naznačen time što se sastoji u sljedećim koracima: (a)dodavanja djelotvorne količine serinske proteaze s endo- i egzoproteolitičkom aktivnošću u uzorak koji sadrži krvnu plazmu ili serum, koja razgrađuje vitamin D-vežuće proteine u krvnoj plazmi ili serumu, tako da više ne mogu vezati metabolite vitamina D; (b)razrjeđivanja navedenog uzorka koji sadrži serinsku proteazu, metabolite vitamina D i razgrađene proteine plazme ili seruma puferom za razrjeđivanje, u kojem je serinska proteaza uglavnom inaktivna; (c)dodavanja spoja za vizualizaciju vitamina D, konjugiranog s čvrstom fazom; (d)dodavanja monoklonskog protutijela za relevantne metabolite vitamina D; (e) kombiniranja uzorka koji sadrži metabolite vitamina D, čvrste faze koja nosi spoj za vizualizaciju vitamina D i monoklonskog protutijela, te provođenja kompeticijskog vezanja kroz prethodno određeni vremenski period između metabolita vitamina D, spoja za vizualizaciju vitamina D vezanog na čvrstu fazu i monoklonskog protutijela u puferu za vezanje, u kojem je serinska proteaza uglavnom inaktivna; (f)izdvajanja čvrste faze koja nosi spoj za vizualizaciju vitamina D i vezano monoklonsko protutijelo iz pufera za vezanje i izbornog ispiranja čvrste faze; i (g)određivanja količine monoklonskog protutijela na čvrstoj fazi i kvantitativnog određivanja metabolita vitamina D u krvnoj plazmi ili serumu uspoređivanjem sa standardnim uzorcima. Patent sadrži još 8 patentnih zahtjeva.
Claims (9)
1. Postupak kvantitativnog određivanja metabolita vitamina D izravno u krvnoj plazmi ili serumu, bez potrebe za prethodnim pročišćavanjem metabolita vitamina D, naznačen time što se sastoji u sljedećim koracima:
(a) dodavanja djelotvorne količine serinske proteaze s endo- i egzoproteolitičkom aktivnošću u uzorak koji sadrži krvnu plazmu ili serum, koja razgrađuje vitamin D-vežuće proteine u krvnoj plazmi ili serumu, tako da više ne mogu vezati metabolite vitamina D;
(b) razrjeđivanja navedenog uzorka koji sadrži serinsku proteazu, metabolite vitamina D i razgrađene proteine plazme ili seruma puferom za razrjeđivanje, u kojem je serinska proteaza uglavnom inaktivna;
(c) dodavanja spoja za vizualizaciju vitamina D, konjugiranog s čvrstom fazom;
(d) dodavanja monoklonskog protutijela za relevantne metabolite vitamina D;
(e) kombiniranja uzorka koji sadrži metabolite vitamina D, čvrste faze koja nosi spoj za vizualizaciju vitamina D i monoklonskog protutijela, te provođenja kompeticijskog vezanja kroz prethodno određeni vremenski period između metabolita vitamina D, spoja za vizualizaciju vitamina D vezanog na čvrstu fazu i monoklonskog protutijela u puferu za vezanje, u kojem je serinska proteaza uglavnom inaktivna;
(f) izdvajanja čvrste faze koja nosi spoj za vizualizaciju vitamina D i vezano monoklonsko protutijelo iz pufera za vezanje i izbornog ispiranja čvrste faze; i
(g) određivanja količine monoklonskog protutijela na čvrstoj fazi i kvantitativnog određivanja metabolita vitamina D u krvnoj plazmi ili serumu uspoređivanjem sa standardnim uzorcima.
2. Postupak u skladu s patentnim zahtjevom 1, naznačen time što serinska proteaza je proteinaza K.
3. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 ili 2, naznačen time što monoklonsko protutijelo se veže na jedan ili više od 25-hidroksivitamina D3, 25-hidroksivitamina D2, 1α,25-dihidroksivitamina D2 i 1α,25-dihidroksivitamina D3.
4. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 3, naznačen time što izravno izmjereni metabolit vitamina D se bira iz skupine koju čine 25-hidroksivitamin D2, 25-hidroksivitamin D3, 1α,25-dihidroksivitamin D2 i 1α,25-dihidroksivitamin D3.
5. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 4, naznačen time što ispitivanje vezanjem proteina je jedno od ELISA (enzimski vezanog imunosorbentnog ispitivanja), RIA (radioimunoispitivanja), FIA (fluorescentnog imunoispitivanja), LIA (luminescentnog imunoispitivanja) ili ILMA.
6. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 5, naznačen time što razgradnju proteina u koraku (a) se provodi u rasponu pH od 6,0 do 10, u prisutnosti 0,1 do 10 mg/ml jednog ili više sredstva za denaturaciju proteina i liberatora vitamina D, koje se bira između salicilne kiseline, toluensulfonske kiseline, naftalensulfonskih kiselina, analinonaftalensulfonskih kiselina, natrijevog dodecil-sulfata i varfarina.
7. Komplet za ispitivanje, namijenjen kvantitativnom određivanju metabolita vitamina D izravno u krvnoj plazmi ili serumu, naznačen time što, u najmanju ruku, sadrži
(i) jedno ili više protutijela specifičnih za metabolite vitamina D,
(ii) spoj za vizualizaciju vitamina D, konjugiran s čvrstom fazom,
(iii) proteinazu K, u matičnoj otopini,
(iv) pufer namijenjen upotrebi s proteinazom K, koji sadrži jedno ili više sredstava za denaturaciju proteina i liberatora vitamina D, koje se bira između salicilne kiseline, toluensulfonske kiseline, naftalensulfonskih kiselina, analinonaftalensulfonskih kiselina, natrijevog dodecil-sulfata i varfarina;
(v) pufer namijenjen upotrebi u ispitivanju s kompetitivnim vezanjem, koji dodatno sadrži inhibitor proteinaze K.
8. Komplet za ispitivanje u skladu s patentnim zahtjevom 7, naznačen time što pufer (v) sadrži 0,1 do 50 mM EGTA i 0,5 do 10 % (tež./tež.) salicilne kiseline i njenih derivata.
9. Komplet za ispitivanje u skladu s patentnim zahtjevom 7, naznačen time što pufer (iv) sadrži denaturacijska sredstva i liberatore vitamina D u količini kojom se dobiva konačne koncentracije od 0,1 do 10 mg/ml.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102007005099 | 2007-02-01 | ||
| PCT/EP2008/051162 WO2008092917A1 (en) | 2007-02-01 | 2008-01-30 | Direct determination of vitamin d in serum or plasma |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20100532T1 true HRP20100532T1 (hr) | 2010-11-30 |
Family
ID=39262786
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HR20100532T HRP20100532T1 (hr) | 2007-02-01 | 2010-09-28 | Izravno određivanje vitamina d u serumu ili plazmi |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US7964363B2 (hr) |
| EP (1) | EP2126586B1 (hr) |
| JP (1) | JP5185291B2 (hr) |
| AT (1) | ATE472734T1 (hr) |
| AU (1) | AU2008209736B2 (hr) |
| DE (1) | DE602008001667D1 (hr) |
| DK (1) | DK2126586T3 (hr) |
| ES (1) | ES2348126T3 (hr) |
| HR (1) | HRP20100532T1 (hr) |
| PL (1) | PL2126586T3 (hr) |
| PT (1) | PT2126586E (hr) |
| WO (1) | WO2008092917A1 (hr) |
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| CN113495159A (zh) * | 2020-03-20 | 2021-10-12 | 郑州达诺生物技术有限公司 | 一种系统反应液、一种25羟基维生素d定量检测试剂盒及其使用方法 |
| CN111521832A (zh) * | 2020-04-27 | 2020-08-11 | 四川沃文特生物技术有限公司 | 一种用于测定25-羟基-维生素d的试剂盒 |
| CN112098663B (zh) * | 2020-09-21 | 2023-03-31 | 上海科华生物工程股份有限公司 | 保持维生素d水溶液稳定的组合物及其制备方法和应用 |
| EP4222502A1 (en) | 2020-09-29 | 2023-08-09 | F. Hoffmann-La Roche AG | A method for determining the level of vitamin d and metabolites thereof |
| CN112485418B (zh) * | 2020-10-26 | 2023-04-11 | 北京利德曼生化股份有限公司 | 一种用于人体维生素b12含量检测的释放剂及其应用 |
| CN113884687B (zh) * | 2021-12-08 | 2022-02-11 | 南京岚轩生物科技有限公司 | 用于检测25-羟基维生素d含量的解离剂及其制备方法 |
| CN115078578A (zh) * | 2022-06-16 | 2022-09-20 | 广州市妇女儿童医疗中心 | 一种基于液质联用的血清维生素d相关代谢物的检测方法 |
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|---|---|---|---|---|
| AU685301B2 (en) * | 1994-07-01 | 1998-01-15 | Akzo Nobel N.V. | A method for monitoring performance of an incubator module, said incubator module being comprised in an automated systemfor assaying multiple samples and a kit suitable for use in said method |
| US6787660B1 (en) * | 1998-06-25 | 2004-09-07 | Immundiagnostik Ag | Functional vitamin D derivatives and a method for determining 25-hydroxy-vitamin D and 1α, dihydroxy-vitamin D |
| GB0029729D0 (en) * | 2000-12-06 | 2001-01-17 | Ids Ltd | Method for detection of vitamin D metabolites |
| US7087395B1 (en) * | 2001-01-16 | 2006-08-08 | Quest Diagnostics Investments Incorporated | Vitamin D assay |
| DE10144905C2 (de) | 2001-09-12 | 2003-07-31 | Immundiagnostik Ag | Bestimmung von Vitamin-D direkt in Serum oder Plasma |
| US20040132104A1 (en) | 2003-01-07 | 2004-07-08 | Sackrison James L. | Vitamin D assay |
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2008
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- 2008-01-30 ES ES08708477T patent/ES2348126T3/es active Active
- 2008-01-30 JP JP2009547690A patent/JP5185291B2/ja active Active
- 2008-01-30 US US12/517,754 patent/US7964363B2/en active Active
- 2008-01-30 AU AU2008209736A patent/AU2008209736B2/en not_active Ceased
- 2008-01-30 AT AT08708477T patent/ATE472734T1/de active
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| EP2126586A1 (en) | 2009-12-02 |
| DE602008001667D1 (de) | 2010-08-12 |
| JP5185291B2 (ja) | 2013-04-17 |
| AU2008209736B2 (en) | 2011-03-03 |
| JP2010518369A (ja) | 2010-05-27 |
| AU2008209736A1 (en) | 2008-08-07 |
| ATE472734T1 (de) | 2010-07-15 |
| ES2348126T3 (es) | 2010-11-30 |
| PL2126586T3 (pl) | 2010-12-31 |
| US20100068725A1 (en) | 2010-03-18 |
| EP2126586B1 (en) | 2010-06-30 |
| PT2126586E (pt) | 2010-09-17 |
| US7964363B2 (en) | 2011-06-21 |
| WO2008092917A1 (en) | 2008-08-07 |
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