HK1204015A1 - Polymerase chain reaction detection system using oligonucleotides comprising a phosphorothioate group - Google Patents

Polymerase chain reaction detection system using oligonucleotides comprising a phosphorothioate group

Info

Publication number
HK1204015A1
HK1204015A1 HK15104355.4A HK15104355A HK1204015A1 HK 1204015 A1 HK1204015 A1 HK 1204015A1 HK 15104355 A HK15104355 A HK 15104355A HK 1204015 A1 HK1204015 A1 HK 1204015A1
Authority
HK
Hong Kong
Prior art keywords
oligonucleotides
detection system
chain reaction
polymerase chain
reaction detection
Prior art date
Application number
HK15104355.4A
Other languages
English (en)
Chinese (zh)
Inventor
Philip Steven Robinson
John Holme
Nisha Jain
Original Assignee
Lgc Genomics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lgc Genomics Ltd filed Critical Lgc Genomics Ltd
Publication of HK1204015A1 publication Critical patent/HK1204015A1/xx

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
HK15104355.4A 2012-03-22 2015-05-07 Polymerase chain reaction detection system using oligonucleotides comprising a phosphorothioate group HK1204015A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/GB2012/050645 WO2013140107A1 (fr) 2012-03-22 2012-03-22 Système de détection de réaction en chaîne de la polymérase utilisant des oligonucléotides comprenant un groupe phosphorothioate

Publications (1)

Publication Number Publication Date
HK1204015A1 true HK1204015A1 (en) 2015-11-06

Family

ID=45894595

Family Applications (1)

Application Number Title Priority Date Filing Date
HK15104355.4A HK1204015A1 (en) 2012-03-22 2015-05-07 Polymerase chain reaction detection system using oligonucleotides comprising a phosphorothioate group

Country Status (19)

Country Link
EP (1) EP2828399B1 (fr)
JP (1) JP6166342B2 (fr)
CN (1) CN104379763B (fr)
AU (1) AU2012374566B2 (fr)
CA (1) CA2867916C (fr)
CY (1) CY1118903T1 (fr)
DK (1) DK2828399T3 (fr)
ES (1) ES2620750T3 (fr)
HK (1) HK1204015A1 (fr)
HR (1) HRP20170569T1 (fr)
HU (1) HUE032212T2 (fr)
IN (1) IN2014DN07878A (fr)
LT (1) LT2828399T (fr)
PL (1) PL2828399T3 (fr)
PT (1) PT2828399T (fr)
RS (1) RS55868B1 (fr)
SG (1) SG11201502154UA (fr)
SI (1) SI2828399T1 (fr)
WO (1) WO2013140107A1 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2515990B (en) 2013-03-06 2016-05-04 Lgc Genomics Ltd Polymerase chain reaction detection system
CN104560972A (zh) * 2014-11-28 2015-04-29 深圳市海普洛斯生物科技有限公司 一种pcr引物及其修饰方法及基于平行高通量单分子高保真扩增方法
CN107208160B (zh) 2015-01-30 2023-02-17 哈佛学院院长及董事 无显微镜成像
CN116949140A (zh) 2016-02-17 2023-10-27 哈佛学院院长及董事 分子编程工具
CN109923213B (zh) * 2016-09-20 2023-02-28 哈佛学院院长及董事 分子验证系统
CN106755379B (zh) * 2016-12-13 2021-06-04 海南医学院 对4种曲霉菌同步定量和基因分型的二聚体突变荧光引物定量pcr方法
RU2698134C2 (ru) 2017-12-04 2019-08-22 Общество с ограниченной ответственностью "Биолабмикс" Способ амплификации нуклеиновых кислот с использованием фосфорилгуанидиновых олигонуклеотидов
GB201713251D0 (en) * 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
US11591645B2 (en) 2017-08-31 2023-02-28 Seegene, Inc. Evaluation of performance of components using a pair of dimer-forming primers
CN111683897A (zh) * 2017-11-03 2020-09-18 华盛顿大学 使用编码颗粒的数字核酸扩增
CN108715888A (zh) * 2018-06-07 2018-10-30 王卫东 一种基于fret的pcr均相检测系统及其应用
CN112342213B (zh) * 2020-10-26 2022-10-11 清华大学 核酸分子及其应用
CN113846187B (zh) * 2021-10-24 2023-07-18 北京宏微特斯生物科技有限公司 利用荧光探针熔解曲线检测待测目标核酸序列的方法及其试剂盒
CN114250281A (zh) * 2021-11-03 2022-03-29 深圳铭毅智造科技有限公司 一种核酸代谢酶活性检测方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5516633A (en) * 1991-08-15 1996-05-14 Amersham Life Science, Inc. DNA sequencing with a T7-type gene 6 exonuclease
CA2176193A1 (fr) * 1995-05-22 1996-11-23 John Wesley Backus Methodes de preamplification de la reaction en chaine de la polymerase a l'aide d'exonucleases, en presence d'amorces a base de phosphorothioate
US6727356B1 (en) 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
GB0510979D0 (en) * 2005-05-28 2005-07-06 Kbiosciences Ltd Detection system for PCR assay
AT502823B1 (de) * 2005-11-29 2007-06-15 Seitz Alexander Dr Polynukleotid-amplifikation

Also Published As

Publication number Publication date
WO2013140107A1 (fr) 2013-09-26
EP2828399A1 (fr) 2015-01-28
CY1118903T1 (el) 2018-01-10
AU2012374566B2 (en) 2018-05-10
DK2828399T3 (en) 2017-04-10
JP2015512251A (ja) 2015-04-27
EP2828399B1 (fr) 2017-01-18
SI2828399T1 (sl) 2017-05-31
HUE032212T2 (en) 2017-09-28
JP6166342B2 (ja) 2017-07-19
LT2828399T (lt) 2017-04-10
ES2620750T3 (es) 2017-06-29
PT2828399T (pt) 2017-04-04
RS55868B1 (sr) 2017-08-31
CN104379763A (zh) 2015-02-25
AU2012374566A1 (en) 2014-10-02
HRP20170569T1 (hr) 2017-06-30
PL2828399T3 (pl) 2017-07-31
CA2867916A1 (fr) 2013-09-26
SG11201502154UA (en) 2015-05-28
CN104379763B (zh) 2017-04-19
AU2012374566A2 (en) 2015-12-24
IN2014DN07878A (fr) 2015-04-24
NZ630915A (en) 2016-08-26
CA2867916C (fr) 2020-05-26

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