HK1142920A1 - 改進的從溶組織梭菌液體培養物純化膠原酶 - Google Patents

改進的從溶組織梭菌液體培養物純化膠原酶

Info

Publication number
HK1142920A1
HK1142920A1 HK10109220.1A HK10109220A HK1142920A1 HK 1142920 A1 HK1142920 A1 HK 1142920A1 HK 10109220 A HK10109220 A HK 10109220A HK 1142920 A1 HK1142920 A1 HK 1142920A1
Authority
HK
Hong Kong
Prior art keywords
collagenases
liquid culture
clostridium histolyticum
improved purification
histolyticum liquid
Prior art date
Application number
HK10109220.1A
Other languages
English (en)
Inventor
Hellmut Eckstein
Michaela Fischer
Werner Hoelke
Antje Liehre
Bernhard Suppmann
Johann-Peter Thalhofer
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of HK1142920A1 publication Critical patent/HK1142920A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
HK10109220.1A 2008-06-02 2010-09-27 改進的從溶組織梭菌液體培養物純化膠原酶 HK1142920A1 (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP08010019 2008-06-02

Publications (1)

Publication Number Publication Date
HK1142920A1 true HK1142920A1 (zh) 2010-12-17

Family

ID=39772928

Family Applications (1)

Application Number Title Priority Date Filing Date
HK10109220.1A HK1142920A1 (zh) 2008-06-02 2010-09-27 改進的從溶組織梭菌液體培養物純化膠原酶

Country Status (9)

Country Link
US (1) US7956167B2 (zh)
EP (1) EP2130551B1 (zh)
JP (1) JP5649800B2 (zh)
KR (1) KR20090125705A (zh)
CN (1) CN101684461B (zh)
CA (1) CA2668118C (zh)
ES (1) ES2388219T3 (zh)
HK (1) HK1142920A1 (zh)
SG (1) SG157346A1 (zh)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10119131B2 (en) * 2011-03-16 2018-11-06 Biospecifics Technologies Corp. Compositions and methods for producing clostridial collagenases
HUE057423T2 (hu) 2012-01-12 2022-05-28 Endo Global Ventures Clostridium histolyticum enzim
DK2968484T3 (da) 2013-03-15 2021-02-22 Biospecifics Tech Corporation Behandlingsfremgangsmåde og produkt til uterus fibromer, som anvender oprenset kollagenase
CN103751102A (zh) 2014-01-15 2014-04-30 上海交通大学 一种胶原酶温敏水凝胶及其制备方法和应用
CN104830821A (zh) * 2015-05-13 2015-08-12 天津商业大学 一种蜡样芽胞杆菌胶原酶的纯化方法
CN105505907B (zh) * 2015-12-18 2019-03-19 中国林业科学研究院林产化学工业研究所 一种没食子酸脱羧酶的分离纯化的方法
EP3497775B1 (en) 2016-09-21 2022-07-13 Tc1 Llc Systems and methods for locating implanted wireless power transmission devices
CN115969318A (zh) 2017-03-01 2023-04-18 恩多风投有限公司 用于评估和治疗橘皮组织的装置和方法
AU2018244426A1 (en) * 2017-03-28 2019-10-17 Endo Ventures Limited Improved method of producing collagenase
CN110412138A (zh) * 2018-04-26 2019-11-05 缪荣明 一种液相色谱检测血清中组织蛋白酶g的方法
CN108949730B (zh) * 2018-07-30 2020-05-12 杭州观苏生物技术有限公司 一种重组变构胶原酶的制备方法及其应用
BR112021012675A2 (pt) * 2019-01-06 2021-09-28 Endo Global Aesthetics Limited Formulação, formulação liofilizada, formulação reconstituída e kit
EP3924363A1 (de) * 2019-02-14 2021-12-22 Nordmark Pharma GmbH Chromatographische aufreinigung von wenigstens einem enzym, ausgesucht aus der gruppe bestehend aus kollagenase typ i, kollagenase typ ii, neutrale protease und clostripain
CN110564709B (zh) * 2019-09-30 2022-12-09 上海开鸿环保科技有限公司 一种同步纯化脂肪酶、酸性蛋白酶和α-淀粉酶的方法
EP4045078A1 (en) 2019-10-15 2022-08-24 The Johns Hopkins University Treatment of uterine fibroids using purified collagenase
CN117347617A (zh) * 2022-06-27 2024-01-05 菲鹏生物股份有限公司 稀释剂及其应用、样品中分析物的测定方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2047306A1 (en) * 1990-07-23 1992-01-24 Milan Holjevac Mutant bacterium clostridium histolyticum, a process for the obtaining thereof, and its use in the production of clostripain-free collagenase
US5332503A (en) * 1993-04-16 1994-07-26 Baxter International Inc. Process for purifying collagenase
WO1996000283A1 (en) * 1994-06-24 1996-01-04 Boehringer Mannheim Corporation A purified mixture of collagenases and two other proteases obtained from clostridium histolyticum
BR9713108A (pt) * 1996-11-19 2004-06-22 Roche Diagnostics Gmbh Colagenase recombinante do tipo i de clostridium histolytucum e o uso da mesma para o isolamento de células e de grupos de células
ATE302844T1 (de) * 2001-07-02 2005-09-15 Nordmark Arzneimittel Gmbh & C Verfahren zur aufreinigung eines enzyms und hiernach hergestelltes, aufgereinigtes enzym sowie verwendung des enzyms
CN1472317A (zh) * 2002-08-01 2004-02-04 辽宁味邦生物制药有限公司 一种用于制备胶原酶的发酵液
US7811560B2 (en) 2006-01-30 2010-10-12 Auxilium Us Holdings, Llc Compositions and methods for treating collagen-mediated diseases

Also Published As

Publication number Publication date
JP5649800B2 (ja) 2015-01-07
CN101684461A (zh) 2010-03-31
CA2668118A1 (en) 2009-12-02
CA2668118C (en) 2016-09-13
EP2130551B1 (en) 2012-06-06
CN101684461B (zh) 2014-08-20
EP2130551A1 (en) 2009-12-09
SG157346A1 (en) 2009-12-29
ES2388219T3 (es) 2012-10-10
US20110070622A1 (en) 2011-03-24
JP2009291195A (ja) 2009-12-17
US7956167B2 (en) 2011-06-07
KR20090125705A (ko) 2009-12-07

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