BR9713108A - Colagenase recombinante do tipo i de clostridium histolytucum e o uso da mesma para o isolamento de células e de grupos de células - Google Patents

Colagenase recombinante do tipo i de clostridium histolytucum e o uso da mesma para o isolamento de células e de grupos de células

Info

Publication number
BR9713108A
BR9713108A BR9713108-3A BR9713108A BR9713108A BR 9713108 A BR9713108 A BR 9713108A BR 9713108 A BR9713108 A BR 9713108A BR 9713108 A BR9713108 A BR 9713108A
Authority
BR
Brazil
Prior art keywords
cell
collagenase
histolytucum
type
recombinant clostridium
Prior art date
Application number
BR9713108-3A
Other languages
English (en)
Inventor
Helmut Burtscher
Dorothee Ambrosius
Friederike Hesse
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Publication of BR9713108A publication Critical patent/BR9713108A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

"COLAGENASE RECOMBINANTE DO TIPO I DE CLOSTRIDIUM HISTOLYTUCUM E O USO DA MESMA PARA O ISOLAMENTO DE CéLULAS E DE GRUPOS DE CéLULAS". Um polipeptídio com as propriedades da colagenase classe I do Clostridium histolyticum (CHC I) e a seq³ência de amino ácido SEQ ID NO: 2 que tem opcionalmente a terminação em N prolongada por um ou diversos amino ácidos da seq³ência SEQ ID NO: 3, é adequado de modo vantajoso para o isolamento de células a partir de tecidos de mamíferos e de tecidos de seres humanos.
BR9713108-3A 1996-11-19 1997-10-30 Colagenase recombinante do tipo i de clostridium histolytucum e o uso da mesma para o isolamento de células e de grupos de células BR9713108A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP96118490 1996-11-19
EP97101085 1997-01-24
PCT/EP1997/006002 WO1998022574A2 (de) 1996-11-19 1997-10-30 Rekombinante collagenase typ i aus clostridium histolyticum und ihre verwendung zur isolierung von zellen und zellverbänden

Publications (1)

Publication Number Publication Date
BR9713108A true BR9713108A (pt) 2004-06-22

Family

ID=26142307

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9713108-3A BR9713108A (pt) 1996-11-19 1997-10-30 Colagenase recombinante do tipo i de clostridium histolytucum e o uso da mesma para o isolamento de células e de grupos de células

Country Status (9)

Country Link
US (1) US6475764B1 (pt)
EP (1) EP0941311B1 (pt)
JP (1) JP2001510331A (pt)
AT (1) ATE272109T1 (pt)
AU (1) AU5222498A (pt)
BR (1) BR9713108A (pt)
CA (1) CA2272244A1 (pt)
DE (1) DE59711812D1 (pt)
WO (1) WO1998022574A2 (pt)

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US6280993B1 (en) * 1999-08-24 2001-08-28 Ichiro Yamato Gene encoding class I collagenase
US7771716B2 (en) 2001-12-07 2010-08-10 Cytori Therapeutics, Inc. Methods of using regenerative cells in the treatment of musculoskeletal disorders
US8105580B2 (en) 2001-12-07 2012-01-31 Cytori Therapeutics, Inc. Methods of using adipose derived stem cells to promote wound healing
US7651684B2 (en) 2001-12-07 2010-01-26 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in augmenting autologous fat transfer
US9597395B2 (en) 2001-12-07 2017-03-21 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in the treatment of cardiovascular conditions
US7585670B2 (en) 2001-12-07 2009-09-08 Cytori Therapeutics, Inc. Automated methods for isolating and using clinically safe adipose derived regenerative cells
US20050095228A1 (en) 2001-12-07 2005-05-05 Fraser John K. Methods of using regenerative cells in the treatment of peripheral vascular disease and related disorders
US20030161816A1 (en) 2001-12-07 2003-08-28 Fraser John K. Systems and methods for treating patients with processed lipoaspirate cells
EP1433845A1 (en) * 2002-12-23 2004-06-30 Georg-August-Universität Clostridium tetani collagenase and uses thereof
MXPA05009044A (es) 2003-02-20 2006-03-17 Macropore Biosurgery Inc Metodos para utilizar celulas derivadas de tejido adiposo en el tratamiento de condiciones cardiovasculares.
DE10331171A1 (de) * 2003-07-09 2005-01-27 Nordmark Arzneimittel Gmbh & Co. Kg Neutrale Protease (NP) und Produkt unter Verwendung von neutraler Protease für Gewebedissoziation sowie Verfahren zu dessen Herstellung
ES2403357T3 (es) 2003-12-11 2013-05-17 Isto Technologies Inc. Sistema de cartílago particulado
US20070231788A1 (en) * 2003-12-31 2007-10-04 Keyvan Behnam Method for In Vitro Assay of Demineralized Bone Matrix
JP5292533B2 (ja) 2005-08-26 2013-09-18 ジンマー・インコーポレイテッド インプラントおよび関節疾患の治療、置換および治療方法
US7811560B2 (en) * 2006-01-30 2010-10-12 Auxilium Us Holdings, Llc Compositions and methods for treating collagen-mediated diseases
US8323642B2 (en) * 2006-12-13 2012-12-04 Depuy Mitek, Inc. Tissue fusion method using collagenase for repair of soft tissue
US8163549B2 (en) 2006-12-20 2012-04-24 Zimmer Orthobiologics, Inc. Method of obtaining viable small tissue particles and use for tissue repair
US20080233614A1 (en) * 2007-02-13 2008-09-25 Cranenburgh Rocky M Production of recombinant collagenases colg and colh in escherichia coli
US20090012629A1 (en) 2007-04-12 2009-01-08 Isto Technologies, Inc. Compositions and methods for tissue repair
US20100159564A1 (en) * 2007-11-30 2010-06-24 Dwulet Francis E Protease resistant recombinant bacterial collagenases
EP2130551B1 (en) * 2008-06-02 2012-06-06 Roche Diagnostics GmbH Improved purification of collagenases from clostridium histolyticum liquid culture
WO2010021993A1 (en) 2008-08-19 2010-02-25 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in the treatment of the lymphatic system and malignant disease
KR101644534B1 (ko) 2009-05-01 2016-08-01 비미니 테크놀로지스 엘엘씨 조직 및 세포 부유화 이식편의 최적화 시스템, 방법 및 조성물
ES2968836T3 (es) 2012-01-12 2024-05-14 Endo Global Ventures Enzima del clostridium histolyticum
US20140178343A1 (en) 2012-12-21 2014-06-26 Jian Q. Yao Supports and methods for promoting integration of cartilage tissue explants
MX2016003127A (es) 2013-09-19 2016-10-28 Cytori Therapeutics Inc Metodos de uso de células derivadas de tejido adiposo en la modulación de dolor y/o fibrosis.
EP3115455A4 (en) * 2014-03-06 2017-09-13 Nippi, Incorporated Grimontia-hollisae-derived recombinant collagenase and enzyme agent for cell separation
IL301796A (en) 2017-03-01 2023-05-01 Endo Ventures Ltd A method for evaluating and treating cellulite
WO2018183582A2 (en) * 2017-03-28 2018-10-04 Endo Ventures Limited Improved method of producing collagenase
WO2020174005A1 (en) 2019-02-28 2020-09-03 The Regenerative Group Ltd. Regenerative combination of plasma and adipose tissue
WO2021200955A1 (ja) 2020-03-31 2021-10-07 天野エンザイム株式会社 コラゲナーゼ剤及びその用途
CN113025599B (zh) * 2021-04-02 2023-09-12 重庆科润生物医药研发有限公司 一种重组溶组织梭菌i型胶原酶及其制备方法和应用
CN112941058B (zh) * 2021-04-02 2023-12-05 重庆科润生物医药研发有限公司 一种重组溶组织梭菌ii型胶原酶及其制备方法和应用
CN118159651A (zh) 2021-09-28 2024-06-07 天野酶制品株式会社 用于制造胶原三肽或者分解软骨的酶剂

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US5801038A (en) * 1984-05-29 1998-09-01 Genencor International Inc. Modified subtilisins having amino acid alterations
US5177011A (en) 1988-08-18 1993-01-05 Calgene, Inc. Plant elongation factor promoters, coding sequences and uses
US5177017A (en) * 1990-03-22 1993-01-05 Trigen, Inc. Molecular cloning of the genes responsible for collagenase production from Clostridium histolyticum
WO1994000580A1 (en) * 1992-06-22 1994-01-06 Trigen, Inc. Molecular cloning of the genes reponsible for collagenase production from clostridium histolyticum
DE4408940A1 (de) * 1994-03-16 1995-10-05 Boehringer Mannheim Gmbh Rekombinante Collagenase Typ II aus Clostridium histolyticum und ihre Verwendung zur Isolierung von Zellen und Zellverbänden

Also Published As

Publication number Publication date
AU5222498A (en) 1998-06-10
WO1998022574A2 (de) 1998-05-28
ATE272109T1 (de) 2004-08-15
US6475764B1 (en) 2002-11-05
EP0941311B1 (de) 2004-07-28
CA2272244A1 (en) 1998-05-28
EP0941311A2 (de) 1999-09-15
DE59711812D1 (de) 2004-09-02
WO1998022574A3 (de) 1998-07-09
JP2001510331A (ja) 2001-07-31

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Legal Events

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 1905 DE 10/07/2007.