HK1137481A1 - A novel vector and expression cell line for mass production of recombinant protein and a process of producing recombinant protein using same - Google Patents

A novel vector and expression cell line for mass production of recombinant protein and a process of producing recombinant protein using same

Info

Publication number
HK1137481A1
HK1137481A1 HK10101256.5A HK10101256A HK1137481A1 HK 1137481 A1 HK1137481 A1 HK 1137481A1 HK 10101256 A HK10101256 A HK 10101256A HK 1137481 A1 HK1137481 A1 HK 1137481A1
Authority
HK
Hong Kong
Prior art keywords
recombinant protein
cell line
same
mass production
producing
Prior art date
Application number
HK10101256.5A
Other languages
English (en)
Inventor
In Young Choi
Chang Hwan Kim
Hyun Ji Lee
Seong Hee Park
Se Chang Kwon
Gwan Sun Lee
Original Assignee
Hanmi Holdings Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hanmi Holdings Co Ltd filed Critical Hanmi Holdings Co Ltd
Publication of HK1137481A1 publication Critical patent/HK1137481A1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C12N9/003—Dihydrofolate reductase [DHFR] (1.5.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
HK10101256.5A 2006-10-16 2010-02-04 A novel vector and expression cell line for mass production of recombinant protein and a process of producing recombinant protein using same HK1137481A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020060100507A KR100880509B1 (ko) 2006-10-16 2006-10-16 재조합 단백질의 대량 생산을 위한 신규한 벡터, 발현세포주 및 이를 이용한 재조합 단백질의 생산 방법
PCT/KR2007/005064 WO2008048037A1 (en) 2006-10-16 2007-10-16 A novel vector and expression cell line for mass production of recombinant protein and a process of producing recombinant protein using same

Publications (1)

Publication Number Publication Date
HK1137481A1 true HK1137481A1 (en) 2010-07-30

Family

ID=39314214

Family Applications (1)

Application Number Title Priority Date Filing Date
HK10101256.5A HK1137481A1 (en) 2006-10-16 2010-02-04 A novel vector and expression cell line for mass production of recombinant protein and a process of producing recombinant protein using same

Country Status (9)

Country Link
US (1) US8957196B2 (de)
EP (1) EP2087118B1 (de)
JP (1) JP5076084B2 (de)
KR (1) KR100880509B1 (de)
CN (1) CN101605899B (de)
AT (1) ATE512229T1 (de)
ES (1) ES2366061T3 (de)
HK (1) HK1137481A1 (de)
WO (1) WO2008048037A1 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101030978B1 (ko) * 2008-07-10 2011-04-28 (주) 에이프로젠 동물세포용 재조합 발현벡터
KR101681513B1 (ko) * 2010-02-04 2016-12-02 한미사이언스 주식회사 인간 난포자극 호르몬의 대량 생산 방법
WO2012057527A2 (en) 2010-10-26 2012-05-03 Hanmi Holdings Co., Ltd. Method for mass production of factor vii/viia
ES2882520T3 (es) 2011-06-17 2021-12-02 Hanmi Science Co Ltd Un conjugado que comprende oxintomodulina y un fragmento de inmunoglobulina y su uso
US9597378B2 (en) 2011-10-06 2017-03-21 Hanmi Science Co., Ltd. Blood coagulation factor VII and VIIa derivatives, conjugates and complexes comprising the same, and use thereof
ES2592263T3 (es) 2012-01-31 2016-11-29 Canvax Biotech, S.L. GPCR con expresión mejorada en superficie celular
TWI641382B (zh) 2013-01-31 2018-11-21 韓美藥品股份有限公司 於包含因子ｖｉｉ之組成物中使病毒失活之方法
KR20150026024A (ko) * 2013-08-30 2015-03-11 한미약품 주식회사 인간 혈액응고 7인자 유도체의 대량 생산 방법
EP3683314A1 (de) * 2013-10-07 2020-07-22 Prestige Biopharma Pte. Ltd. Bicistronischer expressionsvektor zur antikörperexpression und verfahren zur antikörperherstellung damit
RU2548806C1 (ru) * 2014-01-20 2015-04-20 Общество с ограниченной ответственностью "РД-БИОТЕХ" Синтетическая днк, кодирующая эритропоэтин человека, содержащий её вектор, способ получения штамма-продуцента эритропоэтина, штамм-продуцент эритропоэтина
WO2015113494A1 (zh) 2014-01-28 2015-08-06 北京韩美药品有限公司 双功能融合蛋白及其制备方法和用途
KR102418477B1 (ko) 2014-12-30 2022-07-08 한미약품 주식회사 글루카곤 유도체
CN106496302B (zh) * 2015-09-08 2021-12-10 三生国健药业（上海）股份有限公司 一种用离子交换层析纯化蛋白质的方法
CN108753819B (zh) * 2018-05-31 2022-05-20 暨南大学 真核表达载体、真核表达系统、二者的制备方法和应用及gdf11蛋白
EP4276466A1 (de) 2021-01-08 2023-11-15 Beijing Hanmi Pharmaceutical Co., Ltd. Spezifisch an pd-l1 bindender antikörper und antigenbindendes fragment des antikörpers
US20240052037A1 (en) 2021-01-08 2024-02-15 Beijing Hanmi Pharmaceutical Co., Ltd. Anti-pd-l1/anti-cd47 natural antibody structure-like heterodimeric form bispecific antibody and preparation thereof
KR20230129484A (ko) 2021-01-08 2023-09-08 베이징 한미 파마슈티컬 컴퍼니 리미티드 4-1bb와 특이적으로 결합하는 항체 및 그 항원 결합단편

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4399216A (en) 1980-02-25 1983-08-16 The Trustees Of Columbia University Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
KR0162021B1 (ko) * 1995-07-27 1998-11-16 김은영 에리트로포이에틴의 제조방법
KR100232640B1 (ko) * 1997-06-03 1999-12-01 박호군 에리트로포이에틴 (epo)을 생산하는 세포주, 그의 제조방법 및 이를 이용한 epo의 제조방법
PT986644E (pt) * 1997-07-23 2007-01-31 Roche Diagnostics Gmbh Preparação de eritropoietina por activação genética endógena com promotores virais

Also Published As

Publication number Publication date
JP5076084B2 (ja) 2012-11-21
CN101605899A (zh) 2009-12-16
EP2087118A4 (de) 2010-03-03
WO2008048037A1 (en) 2008-04-24
ES2366061T3 (es) 2011-10-14
JP2010506586A (ja) 2010-03-04
KR100880509B1 (ko) 2009-01-28
EP2087118B1 (de) 2011-06-08
KR20080034364A (ko) 2008-04-21
ATE512229T1 (de) 2011-06-15
US20100120089A1 (en) 2010-05-13
US8957196B2 (en) 2015-02-17
CN101605899B (zh) 2013-07-31
EP2087118A1 (de) 2009-08-12

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Legal Events

Date Code Title Description
PC Patent ceased (i.e. patent has lapsed due to the failure to pay the renewal fee)

Effective date: 20211016