HK1098792A1 - Base specific cleavage of methylation-specific amplification products in combination with mass analysis - Google Patents

Base specific cleavage of methylation-specific amplification products in combination with mass analysis

Info

Publication number
HK1098792A1
HK1098792A1 HK07105712.9A HK07105712A HK1098792A1 HK 1098792 A1 HK1098792 A1 HK 1098792A1 HK 07105712 A HK07105712 A HK 07105712A HK 1098792 A1 HK1098792 A1 HK 1098792A1
Authority
HK
Hong Kong
Prior art keywords
nucleic acid
region
methylation
target nucleic
specific amplification
Prior art date
Application number
HK07105712.9A
Other languages
English (en)
Inventor
Mathias Ehrich
Boom Dirk Johannes Van Den
Original Assignee
Sequenom Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sequenom Inc filed Critical Sequenom Inc
Publication of HK1098792A1 publication Critical patent/HK1098792A1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
HK07105712.9A 2004-03-26 2007-05-29 Base specific cleavage of methylation-specific amplification products in combination with mass analysis HK1098792A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55663204P 2004-03-26 2004-03-26
PCT/US2005/009929 WO2005098050A2 (en) 2004-03-26 2005-03-24 Base specific cleavage of methylation-specific amplification products in combination with mass analysis

Publications (1)

Publication Number Publication Date
HK1098792A1 true HK1098792A1 (en) 2007-07-27

Family

ID=35125689

Family Applications (1)

Application Number Title Priority Date Filing Date
HK07105712.9A HK1098792A1 (en) 2004-03-26 2007-05-29 Base specific cleavage of methylation-specific amplification products in combination with mass analysis

Country Status (7)

Country Link
US (1) US9249456B2 (xx)
EP (2) EP2395098B1 (xx)
CN (1) CN101233240A (xx)
AU (1) AU2005230936B2 (xx)
CA (1) CA2561381C (xx)
HK (1) HK1098792A1 (xx)
WO (1) WO2005098050A2 (xx)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6994969B1 (en) * 1999-04-30 2006-02-07 Methexis Genomics, N.V. Diagnostic sequencing by a combination of specific cleavage and mass spectrometry
CA2507189C (en) 2002-11-27 2018-06-12 Sequenom, Inc. Fragmentation-based methods and systems for sequence variation detection and discovery
WO2005024068A2 (en) 2003-09-05 2005-03-17 Sequenom, Inc. Allele-specific sequence variation analysis
EP2395098B1 (en) 2004-03-26 2015-07-15 Agena Bioscience, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
US20070037184A1 (en) * 2005-06-16 2007-02-15 Applera Corporation Methods and kits for evaluating dna methylation
WO2007147063A2 (en) * 2006-06-16 2007-12-21 Sequenom, Inc. Methods and compositions for the amplification, detection and quantification of nucleic acid from a sample
WO2008118988A1 (en) 2007-03-26 2008-10-02 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
CA2684217C (en) * 2007-04-13 2016-12-13 Sequenom, Inc. Comparative sequence analysis processes and systems
AU2009223671B2 (en) 2008-03-11 2014-11-27 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
AU2009228312B2 (en) 2008-03-26 2015-05-21 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
US20110091882A1 (en) * 2009-10-02 2011-04-21 Ibis Biosciences, Inc. Determination of methylation status of polynucleotides
EP3660165B1 (en) 2009-12-22 2023-01-04 Sequenom, Inc. Processes and kits for identifying aneuploidy
US8916344B2 (en) * 2010-11-15 2014-12-23 Exact Sciences Corporation Methylation assay
WO2012109500A2 (en) 2011-02-09 2012-08-16 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
AU2012249531B2 (en) 2011-04-29 2017-06-29 Sequenom, Inc. Quantification of a minority nucleic acid species
US11021703B2 (en) 2012-02-16 2021-06-01 Cornell University Methods and kit for characterizing the modified base status of a transcriptome
EP2820129A1 (en) 2012-03-02 2015-01-07 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20130309667A1 (en) * 2012-05-15 2013-11-21 Anthony P. Shuber Primers for analyzing methylated sequences and methods of use thereof
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US20140093873A1 (en) 2012-07-13 2014-04-03 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
EP2971100A1 (en) 2013-03-13 2016-01-20 Sequenom, Inc. Primers for dna methylation analysis
CN105555972B (zh) 2013-07-25 2020-07-31 伯乐生命医学产品有限公司 遗传测定
CA2938451C (en) * 2014-01-30 2023-10-17 The Regents Of The University Of California Methylation haplotyping for non-invasive diagnosis (monod)
EP3736344A1 (en) 2014-03-13 2020-11-11 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US11216442B2 (en) * 2015-08-13 2022-01-04 H. Lee Moffitt Cancer Center And Research Institute, Inc Large data set negative information storage model
AU2016343937B2 (en) 2015-10-30 2023-01-19 Exact Sciences Corporation Multiplex amplification detection assay and isolation and detection of DNA from plasma
CA3022911A1 (en) 2016-05-05 2017-11-09 Exact Sciences Development Company, Llc Detection of lung neoplasia by analysis of methylated dna
US11208680B2 (en) 2016-07-19 2021-12-28 Exact Sciences Development Company, Llc Nucleic acid control molecules from non-human organisms
EP3574110A4 (en) 2017-01-27 2021-01-13 Exact Sciences Development Company, LLC DETECTION OF COLUMN NEOPLASIA BY ANALYSIS OF METHYLATED DNA
CN111183145B (zh) * 2017-03-08 2024-01-09 芝加哥大学 高灵敏度dna甲基化分析方法
EP3612627A4 (en) * 2017-04-19 2020-12-30 Singlera Genomics, Inc. COMPOSITIONS AND METHODS FOR DETECTION OF GENOMIC VARIANCE AND OF DNA METHYLATION STATUS
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
CN109949866B (zh) * 2018-06-22 2021-02-02 深圳市达仁基因科技有限公司 病原体操作组的检测方法、装置、计算机设备和存储介质
US20210388415A1 (en) * 2018-10-24 2021-12-16 University Of Washington Methods and kits for depletion and enrichment of nucleic acid sequences
JP2022527612A (ja) * 2019-04-09 2022-06-02 エイアールシー バイオ リミテッド ライアビリティ カンパニー ヌクレオチド修飾に基づく枯渇のための組成物及び方法
US11795495B1 (en) * 2019-10-02 2023-10-24 FOXO Labs Inc. Machine learned epigenetic status estimator
CN113817822B (zh) * 2020-06-19 2024-02-13 中国医学科学院肿瘤医院 一种基于甲基化检测的肿瘤诊断试剂盒及其应用

Family Cites Families (203)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US42112A (en) * 1864-03-29 Improvement in grain-drills
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
DE3312929A1 (de) 1982-06-02 1983-12-08 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Verfahren zur sequenzanalyse eines gegebenenfalls modifizierten oligoribonukleotids oder oligodesoxyribonukletids
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5173418A (en) 1985-05-10 1992-12-22 Benzon Pharma, A/S Production in Escherichia coli of extracellular Serratia spp. hydrolases
US4762823A (en) * 1985-10-16 1988-08-09 Sloan-Kettering Institute For Cancer Research Nucleosides of 5-monofluoromethyluracil and 5-difluoromethyluracil
US5079342A (en) * 1986-01-22 1992-01-07 Institut Pasteur Cloned DNA sequences related to the entire genomic RNA of human immunodeficiency virus II (HIV-2), polypeptides encoded by these DNA sequences and use of these DNA clones and polypeptides in diagnostic kits
US4826360A (en) * 1986-03-10 1989-05-02 Shimizu Construction Co., Ltd. Transfer system in a clean room
EP0269520A3 (fr) 1986-11-21 1988-08-24 Institut Pasteur Rétrovirus du type HIV-2 susceptible de provoquer le sida, et ses constituants antigéniques et nucléiques
FR2620049B2 (fr) * 1986-11-28 1989-11-24 Commissariat Energie Atomique Procede de traitement, stockage et/ou transfert d'un objet dans une atmosphere de haute proprete, et conteneur pour la mise en oeuvre de ce procede
US4837726A (en) 1987-06-19 1989-06-06 Applied Biosystems, Inc. Quantitation of chromatographic information
US4802102A (en) 1987-07-15 1989-01-31 Hewlett-Packard Company Baseline correction for chromatography
IE61148B1 (en) 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
DE68927025T2 (de) 1988-06-14 1997-03-27 Qiagen Gmbh HIV-2-Virusvarianten
US5003059A (en) * 1988-06-20 1991-03-26 Genomyx, Inc. Determining DNA sequences by mass spectrometry
EP0395481A3 (en) 1989-04-25 1991-03-20 Spectra-Physics, Inc. Method and apparatus for estimation of parameters describing chromatographic peaks
US5118937A (en) 1989-08-22 1992-06-02 Finnigan Mat Gmbh Process and device for the laser desorption of an analyte molecular ions, especially of biomolecules
US5122457A (en) * 1989-10-19 1992-06-16 Schering Corporation Expression systems utilizing bacteriophage t7 promoters, gene sequences, and t7 rna polymerase
AU7225591A (en) * 1990-01-12 1991-08-05 Scripps Clinic And Research Foundation Nucleic acid enzymes for cleaving dna
NZ236819A (en) * 1990-02-03 1993-07-27 Max Planck Gesellschaft Enzymatic cleavage of fusion proteins; fusion proteins; recombinant dna and pharmaceutical compositions
IT1239733B (it) 1990-02-23 1993-11-15 Eniricerche Spa Mutanti della proteasi neutra termostabili e mezzi e metodi per la loro preparazione
US5288644A (en) 1990-04-04 1994-02-22 The Rockefeller University Instrument and method for the sequencing of genome
CA2079229C (en) 1990-05-09 1999-09-14 Alexander J. Varshavsky Ubiquitin-specific protease
US5264563A (en) 1990-08-24 1993-11-23 Ixsys Inc. Process for synthesizing oligonucleotides with random codons
US5210412A (en) * 1991-01-31 1993-05-11 Wayne State University Method for analyzing an organic sample
US5364759B2 (en) 1991-01-31 1999-07-20 Baylor College Medicine Dna typing with short tandem repeat polymorphisms and identification of polymorphic short tandem repeats
WO1992013629A1 (en) 1991-01-31 1992-08-20 Wayne State University A method for analyzing an organic sample
US5578443A (en) 1991-03-06 1996-11-26 Regents Of The University Of Minnesota DNA sequence-based HLA typing method
CA2066556A1 (en) * 1991-04-26 1992-10-27 Toyoji Sawayanagi Alkaline protease, method for producing the same, use thereof and microorganism producing the same
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
DE69233719T2 (de) 1991-09-24 2009-01-02 Keygene N.V. Primer, Sätze und Restriktionsfragmente und deren Benutzung in selektiver Restriktionsfragmentenamplifikation
IT1260447B (it) 1992-01-27 1996-04-09 Procedimento e apparecchiatura per l'automazione integrale di un laboratorio di analisi
US5436150A (en) * 1992-04-03 1995-07-25 The Johns Hopkins University Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
JPH08500898A (ja) 1992-04-16 1996-01-30 ザ ダウ ケミカル カンパニー 複合データを解釈して異常な器機または処理挙動を検出する改良法
US5646020A (en) 1992-05-14 1997-07-08 Ribozyme Pharmaceuticals, Inc. Hammerhead ribozymes for preferred targets
CA2136717A1 (en) 1992-05-29 1993-12-09 Brian T. Chait Method and product for the sequence determination of peptides using a mass spectrometer
US5792664A (en) 1992-05-29 1998-08-11 The Rockefeller University Methods for producing and analyzing biopolymer ladders
US5440119A (en) * 1992-06-02 1995-08-08 Labowsky; Michael J. Method for eliminating noise and artifact peaks in the deconvolution of multiply charged mass spectra
WO1994000562A1 (en) 1992-06-24 1994-01-06 The Mt. Sinai School Of Medicine Of The City University Of New York A novel human immunodeficiency virus
US5700672A (en) 1992-07-23 1997-12-23 Stratagene Purified thermostable pyrococcus furiousus DNA ligase
EP0596205A3 (en) 1992-11-03 1996-02-21 Hewlett Packard Co Bench supervisor system.
US5795714A (en) 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
US5503980A (en) * 1992-11-06 1996-04-02 Trustees Of Boston University Positional sequencing by hybridization
US6436635B1 (en) * 1992-11-06 2002-08-20 Boston University Solid phase sequencing of double-stranded nucleic acids
US5422253A (en) * 1992-12-07 1995-06-06 Wisconsin Alumni Research Foundation Method of site specific nucleic acid cleavage
WO1994015219A1 (en) 1992-12-23 1994-07-07 Board Of Regents Of The University Of Nebraska Method for automatic testing of laboratory specimens
US6194144B1 (en) * 1993-01-07 2001-02-27 Sequenom, Inc. DNA sequencing by mass spectrometry
DE69433811T2 (de) * 1993-01-07 2005-06-23 Sequenom, Inc., San Diego Dns - sequenzierung durch massenspektronomie
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
AU687801B2 (en) * 1993-03-19 1998-03-05 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
US6074823A (en) * 1993-03-19 2000-06-13 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
US5604098A (en) * 1993-03-24 1997-02-18 Molecular Biology Resources, Inc. Methods and materials for restriction endonuclease applications
WO1994021663A1 (en) 1993-03-24 1994-09-29 Molecular Biology Resources, Inc. Dinucleotide restriction endonuclease preparations and methods of use
US6096715A (en) 1993-05-07 2000-08-01 City Of Hope Chimeric DNA-RNA catalytic sequences
CA2122203C (en) * 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
US5837832A (en) 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
US5861242A (en) * 1993-06-25 1999-01-19 Affymetrix, Inc. Array of nucleic acid probes on biological chips for diagnosis of HIV and methods of using the same
WO1995014108A1 (en) 1993-11-17 1995-05-26 Amersham International Plc Primer extension mass spectroscopy nucleic acid sequencing method
US5908779A (en) * 1993-12-01 1999-06-01 University Of Connecticut Targeted RNA degradation using nuclear antisense RNA
US5538897A (en) 1994-03-14 1996-07-23 University Of Washington Use of mass spectrometry fragmentation patterns of peptides to identify amino acid sequences in databases
US5714330A (en) * 1994-04-04 1998-02-03 Lynx Therapeutics, Inc. DNA sequencing by stepwise ligation and cleavage
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5853979A (en) 1995-06-30 1998-12-29 Visible Genetics Inc. Method and system for DNA sequence determination and mutation detection with reference to a standard
US5498545A (en) * 1994-07-21 1996-03-12 Vestal; Marvin L. Mass spectrometer system and method for matrix-assisted laser desorption measurements
US5453613A (en) 1994-10-21 1995-09-26 Hewlett Packard Company Mass spectra interpretation system including spectra extraction
US5807718A (en) 1994-12-02 1998-09-15 The Scripps Research Institute Enzymatic DNA molecules
US6270962B1 (en) 1995-01-30 2001-08-07 The Regents Of The University Of California Methods for the elimination of DNA sequencing artifacts
US6428955B1 (en) 1995-03-17 2002-08-06 Sequenom, Inc. DNA diagnostics based on mass spectrometry
US5858705A (en) * 1995-06-05 1999-01-12 Human Genome Sciences, Inc. Polynucleotides encoding human DNA ligase III and methods of using these polynucleotides
AU758454B2 (en) 1995-04-11 2003-03-20 Sequenom, Inc. Solid phase sequencing of biopolymers
EP0830460A1 (en) 1995-04-11 1998-03-25 Trustees Of Boston University Solid phase sequencing of biopolymers
US5753439A (en) * 1995-05-19 1998-05-19 Trustees Of Boston University Nucleic acid detection methods
WO1996036986A1 (en) 1995-05-19 1996-11-21 Perseptive Biosystems, Inc. Methods and apparatus for sequencing polymers with a statistical certainty using mass spectrometry
US5869240A (en) * 1995-05-19 1999-02-09 Perseptive Biosystems, Inc. Methods and apparatus for sequencing polymers with a statistical certainty using mass spectrometry
US5874283A (en) 1995-05-30 1999-02-23 John Joseph Harrington Mammalian flap-specific endonuclease
WO1997003210A1 (en) 1995-07-11 1997-01-30 FORFÁS (trading as BIORESEARCH IRELAND) Glycosylase mediated detection of nucleotide sequences at candidate loci
AU6960396A (en) 1995-08-31 1997-03-19 General Hospital Corporation, The Batten disease gene
US6146854A (en) 1995-08-31 2000-11-14 Sequenom, Inc. Filtration processes, kits and devices for isolating plasmids
US5869242A (en) * 1995-09-18 1999-02-09 Myriad Genetics, Inc. Mass spectrometry to assess DNA sequence polymorphisms
US6190865B1 (en) * 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules
US6090549A (en) 1996-01-16 2000-07-18 University Of Chicago Use of continuous/contiguous stacking hybridization as a diagnostic tool
US6090606A (en) * 1996-01-24 2000-07-18 Third Wave Technologies, Inc. Cleavage agents
US5830712A (en) 1996-02-06 1998-11-03 Allelix Biopharmaceuticals Inc. Selective template deletion method
US5686656A (en) 1996-02-27 1997-11-11 Aviv Amirav Method and device for the introduction of a sample into a gas chromatograph
US6051378A (en) * 1996-03-04 2000-04-18 Genetrace Systems Inc. Methods of screening nucleic acids using mass spectrometry
AU2217597A (en) 1996-03-18 1997-10-22 Sequenom, Inc. Dna sequencing by mass spectrometry
DE19613082C2 (de) 1996-04-02 1999-10-21 Koenig & Bauer Ag Verfahren und Vorrichtung zur qualitativen Beurteilung von bearbeitetem Material
AU739256B2 (en) 1996-04-08 2001-10-04 Glaxo Group Limited Mass-based encoding and qualitative analysis of combinatorial libraries
JP2000508912A (ja) 1996-04-19 2000-07-18 スペクトラ バイオメディカル，インコーポレイテッド 多型形態と複数の表現型との相関付け
US5928906A (en) 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
US6022688A (en) 1996-05-13 2000-02-08 Sequenom, Inc. Method for dissociating biotin complexes
US5786146A (en) 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
US6017704A (en) * 1996-06-03 2000-01-25 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
EP0954611A1 (en) 1996-06-10 1999-11-10 University Of Utah Research Foundation Rapid, accurate identification of dna sequence variants by electrospray mass spectrometry
DE69734828T2 (de) * 1996-06-10 2006-10-26 Novozymes, Inc., Davis 5-aminolevulinsaure synthase aus aspergillus oryzae und dafuer kodierende nukleinsaeure
FR2749662B1 (fr) 1996-06-11 1998-08-28 Elf Aquitaine Laboratoire robotise d'analyses d'echantillons
WO1998003684A1 (en) 1996-07-19 1998-01-29 Hybridon, Inc. Method for sequencing nucleic acids using matrix-assisted laser desorption ionization time-of-flight mass spectrometry
US6008334A (en) 1996-07-24 1999-12-28 The Board Of Regents Of The University Of Oklahoma Base-protected nucleotide analogs with protected thiol groups
US5928870A (en) * 1997-06-16 1999-07-27 Exact Laboratories, Inc. Methods for the detection of loss of heterozygosity
GB9618960D0 (en) * 1996-09-11 1996-10-23 Medical Science Sys Inc Proteases
US5885841A (en) * 1996-09-11 1999-03-23 Eli Lilly And Company System and methods for qualitatively and quantitatively comparing complex admixtures using single ion chromatograms derived from spectroscopic analysis of such admixtures
US5849546A (en) 1996-09-13 1998-12-15 Epicentre Technologies Corporation Methods for using mutant RNA polymerases with reduced discrimination between non-canonical and canonical nucleoside triphosphates
US5965363A (en) * 1996-09-19 1999-10-12 Genetrace Systems Inc. Methods of preparing nucleic acids for mass spectrometric analysis
US5777324A (en) 1996-09-19 1998-07-07 Sequenom, Inc. Method and apparatus for maldi analysis
WO1998012355A1 (en) 1996-09-19 1998-03-26 Genetrace Systems Methods of preparing nucleic acids for mass spectrometric analysis
US5864137A (en) * 1996-10-01 1999-01-26 Genetrace Systems, Inc. Mass spectrometer
US6091424A (en) 1996-11-01 2000-07-18 Tom Sawyer Software Labeling graphical features of drawings
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US6140053A (en) 1996-11-06 2000-10-31 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
CA2267070A1 (en) 1996-11-06 1998-05-14 Sequenom, Inc. Compositions and methods for immobilizing nucleic acids to solid supports
ES2215241T3 (es) 1996-11-06 2004-10-01 Sequenom, Inc. Procedimiento de espectrometria de masa.
US6024925A (en) * 1997-01-23 2000-02-15 Sequenom, Inc. Systems and methods for preparing low volume analyte array elements
CA2270132A1 (en) 1996-11-06 1998-05-14 Sequenom, Inc. Dna diagnostics based on mass spectrometry
US5900481A (en) * 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
WO1998024935A1 (en) 1996-12-06 1998-06-11 Urocor, Inc. Diagnosis of disease state using mrna profiles
US6297006B1 (en) 1997-01-16 2001-10-02 Hyseq, Inc. Methods for sequencing repetitive sequences and for determining the order of sequence subfragments
CA2284463A1 (en) 1997-02-04 1998-08-06 Hubert Koster A reversible stoichiometric process for conjugating biomolecules
US6059724A (en) 1997-02-14 2000-05-09 Biosignal, Inc. System for predicting future health
AU6553498A (en) 1997-03-14 1998-09-29 Hybridon, Inc. Method for sequencing of modified nucleic acids using electrospray ionization-fourier transform mass spectrometry
WO1998054571A1 (en) 1997-05-28 1998-12-03 The Walter And Eliza Hall Institute Of Medical Research Nucleic acid diagnostics based on mass spectrometry or mass separation and base specific cleavage
US5976806A (en) 1997-06-25 1999-11-02 Pioneer Hi-Bred International, Inc. DNA ligase assay
US5975492A (en) 1997-07-14 1999-11-02 Brenes; Arthur Bellows driver slot valve
DE69823206T2 (de) 1997-07-25 2004-08-19 Affymetrix, Inc. (a Delaware Corp.), Santa Clara Verfahren zur herstellung einer bio-informatik-datenbank
US6207370B1 (en) 1997-09-02 2001-03-27 Sequenom, Inc. Diagnostics based on mass spectrometric detection of translated target polypeptides
US5888795A (en) * 1997-09-09 1999-03-30 Becton, Dickinson And Company Thermostable uracil DNA glycosylase and methods of use
US6112161A (en) 1997-09-17 2000-08-29 Hewlett-Packard Method, apparatus, and article of manufacture for enhanced intergration of signals
WO1999014375A2 (en) * 1997-09-19 1999-03-25 Genetrace Systems, Inc. Dna typing by mass spectrometry with polymorphic dna repeat markers
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US5932451A (en) 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
US5948902A (en) 1997-11-20 1999-09-07 South Alabama Medical Science Foundation Antisense oligonucleotides to human serine/threonine protein phosphatase genes
DE19754482A1 (de) * 1997-11-27 1999-07-01 Epigenomics Gmbh Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
US6537746B2 (en) * 1997-12-08 2003-03-25 Maxygen, Inc. Method for creating polynucleotide and polypeptide sequences
US6268131B1 (en) 1997-12-15 2001-07-31 Sequenom, Inc. Mass spectrometric methods for sequencing nucleic acids
DE19803309C1 (de) * 1998-01-29 1999-10-07 Bruker Daltonik Gmbh Massenspektrometrisches Verfahren zur genauen Massenbestimmung unbekannter Ionen
US6054276A (en) * 1998-02-23 2000-04-25 Macevicz; Stephen C. DNA restriction site mapping
WO1999050447A1 (de) 1998-03-27 1999-10-07 Mira Diagnostika Gmbh Verfahren und nucleinsäureverbindung zum abbau von in-vitro synthetisierten nucleinsäuremolekülen
JP3575295B2 (ja) 1998-04-15 2004-10-13 住友電装株式会社 電気接続用コネクタのプラグ
EP1071811B8 (en) 1998-04-22 2003-01-02 Enterprise Ireland (trading as Bioresearch Ireland) A method for the characterisation of nucleic acid molecules involving generation of extendible upstream dna fragments resulting from the cleavage of nucleic acid at an abasic site
US6723564B2 (en) * 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices
US20030017483A1 (en) * 1998-05-12 2003-01-23 Ecker David J. Modulation of molecular interaction sites on RNA and other biomolecules
US6099553A (en) 1998-05-21 2000-08-08 Applied Medical Resources Corporation Suture clinch
US6104028A (en) * 1998-05-29 2000-08-15 Genetrace Systems Inc. Volatile matrices for matrix-assisted laser desorption/ionization mass spectrometry
GB9827152D0 (en) 1998-07-03 1999-02-03 Devgen Nv Characterisation of gene function using double stranded rna inhibition
JP2000067805A (ja) * 1998-08-24 2000-03-03 Hitachi Ltd 質量分析装置
EP1117838B1 (en) 1998-10-01 2009-05-13 Variagenics, Inc. A method for analyzing polynucleotides
US6440705B1 (en) 1998-10-01 2002-08-27 Vincent P. Stanton, Jr. Method for analyzing polynucleotides
EP1953229A3 (en) 1998-10-15 2008-12-24 Novartis Vaccines and Diagnostics, Inc. Metastatic breast and colon cancer regulated genes
US6270835B1 (en) 1999-10-07 2001-08-07 Microcoating Technologies, Inc. Formation of this film capacitors
AU1739500A (en) 1998-11-24 2000-06-13 Johns Hopkins University, The Genotyping by mass spectrometric analysis of short dna fragments
GB9904585D0 (en) 1999-02-26 1999-04-21 Gemini Research Limited Clinical and diagnostic database
US6225061B1 (en) 1999-03-10 2001-05-01 Sequenom, Inc. Systems and methods for performing reactions in an unsealed environment
US6331427B1 (en) 1999-03-26 2001-12-18 Millennium Pharmaceuticals, Inc. Protease homologs
US20020009394A1 (en) 1999-04-02 2002-01-24 Hubert Koster Automated process line
US6309833B1 (en) 1999-04-12 2001-10-30 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures
JP5615997B2 (ja) 1999-04-30 2014-10-29 シークエノム・インコーポレーテツド 特異的切断および質量分析法の組合せによる診断的配列決定
US6994969B1 (en) 1999-04-30 2006-02-07 Methexis Genomics, N.V. Diagnostic sequencing by a combination of specific cleavage and mass spectrometry
EP1261932B1 (en) 1999-10-13 2009-09-30 Sequenom, Inc. Methods for identifying polymorphic genetic markers
US7917301B1 (en) 2000-09-19 2011-03-29 Sequenom, Inc. Method and device for identifying a biological sample
US7332275B2 (en) * 1999-10-13 2008-02-19 Sequenom, Inc. Methods for detecting methylated nucleotides
AU2001233176A1 (en) 2000-01-31 2001-08-07 Shengfang Jin Resistance sequences and uses thereof
US6475736B1 (en) 2000-05-23 2002-11-05 Variagenics, Inc. Methods for genetic analysis of DNA using biased amplification of polymorphic sites
GB0019499D0 (en) 2000-08-08 2000-09-27 Diamond Optical Tech Ltd system and method
EP1179589A1 (en) 2000-08-08 2002-02-13 F. Hoffmann-La Roche Ag MMX-1, a member of the family of human cancer/testis antigens, a protein encoded thereby and a process for determining whether a tumor sample has metastatic potential
US20030027169A1 (en) * 2000-10-27 2003-02-06 Sheng Zhang One-well assay for high throughput detection of single nucleotide polymorphisms
AU2002221800A1 (en) 2000-11-02 2002-05-15 Epigenomics Ag Systems, methods and computer program products for guiding the selection of therapeutic treatment regimens
DE10061348C2 (de) 2000-12-06 2002-10-24 Epigenomics Ag Verfahren zur Quantifizierung von Cytosin-Methylierungen in komplex amplifizierter genomischer DNA
DE10112515B4 (de) * 2001-03-09 2004-02-12 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität
US20030013099A1 (en) * 2001-03-19 2003-01-16 Lasek Amy K. W. Genes regulated by DNA methylation in colon tumors
US7056663B2 (en) * 2001-03-23 2006-06-06 California Pacific Medical Center Prognostic methods for breast cancer
US20040102905A1 (en) * 2001-03-26 2004-05-27 Epigenomics Ag Method for epigenetic feature selection
US6522477B2 (en) * 2001-04-17 2003-02-18 Karl Storz Imaging, Inc. Endoscopic video camera with magnetic drive focusing
US20020155587A1 (en) 2001-04-20 2002-10-24 Sequenom, Inc. System and method for testing a biological sample
WO2002086163A1 (en) 2001-04-20 2002-10-31 Karolinska Innovations Ab Methods for high throughput genome analysis using restriction site tagged microarrays
EP1402071A4 (en) 2001-06-08 2005-12-14 Us Genomics Inc METHOD AND PRODUCTS FOR THE ANALYSIS OF NUCLEIC ACIDS BASED ON THE METHYLATION STATUS
DE10130800B4 (de) 2001-06-22 2005-06-23 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung mit hoher Sensitivität
DE10132212B4 (de) 2001-06-27 2005-11-24 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung durch vergleichende Analyse der Einzelstränge von Amplifikaten
CA2492007A1 (en) 2001-07-15 2003-01-30 Keck Graduate Institute Amplification of nucleic acid fragments using nicking agents
DE10151055B4 (de) 2001-10-05 2005-05-25 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung in CpG Inseln
DE10154317B4 (de) 2001-10-26 2005-06-09 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben
WO2003038726A2 (en) 2001-10-31 2003-05-08 Epigenomics Ag A method for epigenetic knowledge generation
JP4381142B2 (ja) 2001-11-16 2009-12-09 ザ ジョーンズ ホプキンス ユニバーシティー スクール オブ メディシン 前立腺癌の検出法
JP2003245087A (ja) 2001-11-22 2003-09-02 Sankyo Co Ltd 遺伝子診断方法
DE10201138B4 (de) 2002-01-08 2005-03-10 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungsmustern durch exponentielle Ligation hybridisierter Sondenoligonukleotide (MLA)
WO2003078593A2 (en) 2002-03-15 2003-09-25 Epigenomics Ag Discovery and diagnostic methods using 5-methylcytosine dna glycosylase
EP1344832A1 (en) 2002-03-15 2003-09-17 Epigenomics AG Methods and nucleic acids for the analysis of methylation within the gene melastatin
WO2003080863A1 (en) * 2002-03-25 2003-10-02 Epigenomics Ag Method for the analysis of methylation patterns within nucleic acids by means of mass spectrometry
AU2003228514A1 (en) * 2002-04-11 2003-10-27 Sequenom, Inc. Methods and devices for performing chemical reactions on a solid support
US20040014101A1 (en) 2002-05-03 2004-01-22 Pel-Freez Clinical Systems, Inc. Separating and/or identifying polymorphic nucleic acids using universal bases
WO2004013284A2 (en) 2002-08-02 2004-02-12 Tufts University A method to assess genomic dna methylation using high-performance liquid chromatography-electospray ionization mass spectrometry
CA2507189C (en) * 2002-11-27 2018-06-12 Sequenom, Inc. Fragmentation-based methods and systems for sequence variation detection and discovery
AU2004235331B2 (en) * 2003-04-25 2008-12-18 Sequenom, Inc. Fragmentation-based methods and systems for De Novo sequencing
US20050009059A1 (en) 2003-05-07 2005-01-13 Affymetrix, Inc. Analysis of methylation status using oligonucleotide arrays
US8150626B2 (en) * 2003-05-15 2012-04-03 Illumina, Inc. Methods and compositions for diagnosing lung cancer with specific DNA methylation patterns
WO2004110246A2 (en) * 2003-05-15 2004-12-23 Illumina, Inc. Methods and compositions for diagnosing conditions associated with specific dna methylation patterns
US20040253141A1 (en) 2003-06-16 2004-12-16 Schembri Carol T. Apparatus and method for nucleic acid spatial ordering
WO2005024068A2 (en) * 2003-09-05 2005-03-17 Sequenom, Inc. Allele-specific sequence variation analysis
ES2383970T3 (es) 2003-10-21 2012-06-27 Orion Genomics, Llc Fragmentación enzimática diferencial
US20090075251A1 (en) 2004-03-24 2009-03-19 Dimo Dietrich Method for analysis of cytosine methylation
EP2395098B1 (en) 2004-03-26 2015-07-15 Agena Bioscience, Inc. Base specific cleavage of methylation-specific amplification products in combination with mass analysis
US7608394B2 (en) 2004-03-26 2009-10-27 Sequenom, Inc. Methods and compositions for phenotype identification based on nucleic acid methylation
CA2580070A1 (en) * 2004-09-10 2006-03-23 Sequenom, Inc. Methods for long-range sequence analysis of nucleic acids
WO2007016668A2 (en) 2005-08-02 2007-02-08 Sequenom, Inc. Methods and compositions for disease prognosis based on nucleic acid methylation

Also Published As

Publication number Publication date
CN101233240A (zh) 2008-07-30
EP2395098B1 (en) 2015-07-15
CA2561381A1 (en) 2005-10-20
US9249456B2 (en) 2016-02-02
EP1727911A4 (en) 2009-03-04
EP2395098A1 (en) 2011-12-14
AU2005230936B2 (en) 2010-08-05
WO2005098050A2 (en) 2005-10-20
EP1727911A2 (en) 2006-12-06
WO2005098050A3 (en) 2007-11-08
AU2005230936A1 (en) 2005-10-20
US20050272070A1 (en) 2005-12-08
EP1727911B1 (en) 2013-01-23
CA2561381C (en) 2015-05-12

Similar Documents

Publication Publication Date Title
HK1098792A1 (en) Base specific cleavage of methylation-specific amplification products in combination with mass analysis
Ammerpohl et al. Hunting for the 5th base: Techniques for analyzing DNA methylation
CY1118000T1 (el) Διαδικασια υψηλης παροχης για αναλυση μεθυλιωσης dna
WO2001098528A3 (de) Verfahren zum nachweis von cytosin-methylierungen
US20220145357A1 (en) Method of nucleic acid enrichment using site-specific nucleases followed by capture
DE69916877D1 (de) Ligationsassemblierung und detektion von polynucleotiden auf einem festträger
ATE395434T1 (de) Verfahren zur gleichzeitigen detektion von cytosin-methylierung und mutationen in dna proben
DE50212159D1 (de) Verfahren zum nachweis von cytosin-methylierung in cpg-inseln
ATE464397T1 (de) Cpg-amplicon und array-protokoll
AU757473B2 (en) Method for identifying cytosine methylation patterns in genomic DNA
WO2016034908A1 (en) Methods for detection of nucleotide modification
DE50112799D1 (de) Verfahren zum nachweis von cytosin-methylierungen
JP2023508795A (ja) Ｄｎａおよびｒｎａ修飾の濃縮および検出のための方法およびキット、ならびに機能モチーフ
DE602004004988D1 (de) Methylierungsstatus-Detektionsassays mittels methylierungsspezifischer Primerextension (MSPE)
WO2001062960A3 (de) Verfahren zur detektion von cytosin-methylierung in dna proben
NO20011607D0 (no) Fremgangsmåte for analysering av polynukleotider
WO2004003232A8 (en) Method for the characterisation of nucleic acid molecules
AU775085B2 (en) Method for distinguishing 5-position methylation changes
WO2001029262A3 (en) Genotyping reagents, kits and methods of use thereof
WO2008009365A3 (en) A method for determining the methylation rate of a nucleic acid
WO2003002752A3 (en) Methods of using nick translate libraries for snp analysis
US20060099581A1 (en) Method for analysis of methylated nucleic acids
WO2004067775A3 (en) Method for the detection of cytosine methylation patterns with high sensitivity
ATE455864T1 (de) Verfahren zum nachweis von in einer polymerase- kettenreaktion amplifizierten nukleinsäuremolekülen
EP1704255B1 (en) Method for investigating cytosine methylation in dna by means of dna repair enzymes

Legal Events

Date Code Title Description
PC Patent ceased (i.e. patent has lapsed due to the failure to pay the renewal fee)

Effective date: 20200323